General


Ya-Jun Liu, Ph.D
​Professor, Metabolomics Group
Qingdao Institute of Bioenergy and Bioprocess Technology
Chinese Academy of Sciences
Address: 189 Songling Rd., Qingdao 266101, P. R. China
Phone: +86-532-80662705
Email: liuyj@qibebt.ac.cn

Research Areas

  • Genetic engineering of cellulolytic bacteria
  • Cellulosome function
  • Lignocellulose saccharification process
  • Plastic bio-recycling

Education

2001.09-2005.07       B.S. 

College of Marine Life Science, Ocean University of China, P.R.China (Biology Science)
2005.08-2011.01       Ph.D. (M.A.-Ph.D. Program)
State Key Laboratory of Microbial Resource at Institute of Microbiology, Institute of Microbiology, Chinese Academy of Sciences, P.R.China (Microbiology)
2007.12-2010.02       Co-Education (DAAD-CAS Joint Scholarship)
Department of Ecological Microbiology, University of Bayreuth, Germany (Ecological Microbiology)

Experience

2009-2010 Student research assistant 
Department of Ecological Microbiology, University of Bayreuth, Germany 
2011-2013 Assistant professor
Metabolomics Group, Qingdao Institute of Bioenergy and Bioprocess Technology (QIBEBT, CAS)
2014-2020 Associate professor
Metabolomics Group, Qingdao Institute of Bioenergy and Bioprocess Technology (QIBEBT, CAS)

2020-now Professor

Metabolomics Group, Qingdao Institute of Bioenergy and Bioprocess Technology (QIBEBT, CAS)

Publications

(1) Coordinated β-glucosidase activity with the cellulosome is effective for enhanced lignocellulose saccharification, Bioresource Technology, 2022, 通讯作者
(2) Dissolved xylan inhibits cellulosome-based saccharification by binding to the key cellulosomal component of Clostridium thermocellum, International Journal of Biological Macromolecules, 2022, 通讯作者
(3) Biological cellulose saccharification using a coculture of Clostridium thermocellum and Thermobrachium celere strain A9, Applied Microbiology and Biotechnology, 2022, 
(4) Structural insight into a GH1 β-glucosidase from the oleaginous microalga, Nannochloropsis oceanica, International Journal of Biological Macromolecules, 2021, 第 2 作者
(5) Thermophilic whole-cell degradation of polyethylene terephthalate using engineered Clostridium thermocellum, MICROBIAL BIOTECHNOLOGY, 2021, 通讯作者
(6) Response characteristics of the membrane integrity and physiological activities of the mutant strain Y217 under exogenous butanol stress, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2021, 第 4 作者
(7) NMR chemical shift assignments of a module of unknown function in the cellulosomal secondary scaffoldin ScaF from Clostridium thermocellum, BIOMOLECULAR NMR ASSIGNMENTS, 2021, 第 3 作者
(8) Discovery and mechanism of a pH-dependent dual-binding-site switch in the interaction of a pair of protein modules, SCIENCE ADVANCES, 2020, 第 5 作者
(9) Consolidated bio-saccharification: Leading lignocellulose bioconversion into the real world, BIOTECHNOLOGY ADVANCES, 2020, 通讯作者
(10) Impact of ammonium sulfite-based sequential pretreatment combinations on two distinct saccharifications of wheat straw, RSC ADVANCES, 2020, 通讯作者
(11) Robust production of pigment-free pullulan from lignocellulosic hydrolysate by a new fungus co-utilizing glucose and xylose, CARBOHYDRATE POLYMERS, 2020, 通讯作者
(12) Solution structure of a unicellular microalgae-derived translationally controlled tumor protein revealed both conserved features and structural diversity, ARCHIVES OF BIOCHEMISTRY AND BIOPHYSICS, 2019, 第 2 作者
(13) Construction of consolidated bio-saccharification biocatalyst and process optimization for highly efficient lignocellulose solubilization, BIOTECHNOLOGY FOR BIOFUELS, 2019, 第 2 作者
(14) Alternative sigma(I)/anti-sigma(I) factors represent a unique form of bacterial sigma/anti-sigma complex, NUCLEIC ACIDS RESEARCH, 2019, 第 3 作者
(15) Enhancing tricarboxylate transportation-related NADPH generation to improve biodiesel production by Aurantiochytrium, ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS, 2019, 第 4 作者
(16) Determination of the native features of the exoglucanase Cel48S from Clostridium thermocellum, BIOTECHNOLOGY FOR BIOFUELS, 2018, 通讯作者
(17) Firmicutes-enriched IS1447 represents a group of IS3-family insertion sequences exhibiting unique+1 transcriptional slippage, BIOTECHNOLOGY FOR BIOFUELS, 2018, 第 1 作者
(18) Regulation of biomass degradation by alternative sigma factors in cellulolytic clostridia, SCIENTIFIC REPORTS, 2018, 第 3 作者
(19) Efficient whole-cell-catalyzing cellulose saccharification using engineered Clostridium thermocellum, BIOTECHNOLOGY FOR BIOFUELS, 2017, 通讯作者
(20) Low stability of the reduced state of Mycobacterium tuberculosis NrdH redoxin, FEBS LETTERS, 2016, 第 1 作者
(21) Overexpression of glucose-6-phosphate dehydrogenase enhanced the polyunsaturated fatty acid composition of Aurantiochytrium sp SD116, ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS, 2016, 第 3 作者
(22) Integration of bacterial expansin-like proteins into cellulosome promotes the cellulose degradation, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2016, 第 4 作者
(23) A novel arabinose-inducible genetic operation system developed for Clostridium cellulolyticum, BIOTECHNOLOGY FOR BIOFUELS, 2015, 通讯作者
(24) Current progress of targetron technology: Development, improvement and application in metabolic engineering, BIOTECHNOLOGY JOURNAL, 2015, 第 1 作者
(25) 典型产纤维小体梭菌的遗传改造及其在纤维素乙醇中的应用研究进展, Genetic engineering of cellulosome-producing Clostridium strains for cellulosic ethanol production, 生物加工过程, 2014, 第 1 作者
(26) Improvement of ClosTron for successive gene disruption in Clostridium cellulolyticum using a pyrF-based screening system, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2014, 第 7 作者
(27) The contribution of cellulosomal scaffoldins to cellulose hydrolysis by Clostridium thermocellum analyzed by using thermotargetrons, BIOTECHNOLOGY FOR BIOFUELS, 2014, 通讯作者
(28) Different Impacts of Short-Chain Fatty Acids on Saturated and Polyunsaturated Fatty Acid Biosynthesis in Aurantiochytrium sp SD116, JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2013, 第 3 作者
(29) A Targetron System for Gene Targeting in Thermophiles and Its Application in Clostridium thermocellum, PLOS ONE, 2013, 通讯作者
(30) Targeted gene engineering in Clostridium cellulolyticum H10 without methylation, JOURNAL OF MICROBIOLOGICAL METHODS, 2012, 通讯作者

Research Interests

  • Genetic manipulation system construction for non-model microorganisms.
  • Metabolic engineering of Clostridia for lignocellulose bioconversion.
  • Study on the function and regulation mechanism of cellulosome
  • Development of whole-cell biocatalysts for plastic waste bioremediation and bio-recycling.

    Conferences

    (1)研究生论坛点评专家   第七届生物质能源国际会议暨第十五届全国研究生生物质能研讨会   2022-04-08
    (2)From Straw to Feed: A Novel Bioconversion Strategy via Consolidated Bio-Saccharification   2022-02-14
    (3)全新木质纤维素整合生物糖化体系 的建立与解析   2021年中国微生物学会学术年会   2021-10-29
    (4)Development of thermophilic Clostridium chassis for lignocellulose biodegradation   2021第三届国际合成生物学论坛   2021-06-21
    (5)全新的高温全菌催化PET塑料生物降解策略   2020年中国微生物学会学术年会   2020-10-23
    (6)From Lignocellulose to Single-cell Oil based on Consolidated Bio-Saccharification   2020-06-29
    (7)基于高效全菌催化剂的木质纤维素整合生物糖化   2019年中国微生物学会学术年会   2019-10-13
    (8)Efficient consolidated biosaccharification of lignocellulose using engineered whole-cell catalyst   第五届国际纸浆造纸与生物技术会议   2018-11-12
    (9)Efficient consolidated bio-saccharification of lignocellulose using engineered whole-cell catalysts   第十五届国际梭菌会议   2018-09-18
    (10)基于全菌催化剂的整合生物糖化及CelS天然性质解析   2017年微生物学会年会   2017-10-20
    (11)Efficient whole-cell catalyst for cellulose saccharification   中英梭菌会议   2016-12-05
    (12)The Contribution of Cellulosomal Scaffoldins to Cellulose Hydrolysis by Clostridium thermocellum    第十三届国际梭菌会议   刘亚君   2014-09-19
    (13)热纤梭菌纤维小体的功能分析,   2014年微生物学会年会   刘亚君   2014-08-16
    (14)The genetic manipulation of Clostridium thermocellum for cellulosic ethanol production   2013年中国微生物学会学术年会   刘亚君,洪伟,张杰,崔古贞,崔球   2013-10-25


    Collaboration

    Professor Edward A. Bayer,Weizmann Institute of Science, Israel
    Professor Alan Lambowitz, University of Texas at Austin, USA
    Professor Akihiko Kosugi, Japan International Research Center for Agricultural Sciences, Japan

    Dr. Ren Wei, University of Greifswald, Germany