071010-生物化学与分子生物学

纳米抗体，体外诊断，靶向药物

2011-09--2016-06   中国农业大学   博士研究生
2007-09--2011-06   山东农业大学   学士

   

2020-01~现在, 中国科学院青岛生物能源与过程研究所, 副研究员
2018-10~2020-01,中国科学院青岛生物能源与过程研究所, 助理研究员
2016-07~2018-10,中国科学院青岛生物能源与过程研究所, 博士后

   

[1] 年锐, 郭杨, 刘文帅, 于建立, 樊喜英. 一种可提高蛋白表达量的丝素蛋白序列标签及其应用. CN114085279A, 2022-02-25.
[2] 年锐, 王婷婷, 樊喜英, 刘文帅, 古一, 孙国川, 牟汝华. 一种纳米抗体介导的双重肿瘤靶向介孔二氧化硅纳米载药制剂及其制备方法. 2021114892733, 2021-12-08.
[3] 孙粤, 包子娴, 年锐, 莱东, 咸漠, 刘文帅, 樊喜英, 陈泉. 一种用于生产磺基化重组水蛭素的重组菌及其制备方法与应用. CN: CN113699086A, 2021-11-26.
[4] 年锐, 刘文帅, 郭杨, 于建立, 樊喜英. 一种提高纳米抗体表达量的方法. 2021112828836, 2021-11-01.
[5] 包子娴, 孙粤, 年锐, 咸漠, 樊喜英, 陈泉, 刘文帅, 彭馨莹. 一种强粘附性、高机械强度的PEGDA-贻贝粘附蛋白-胶原蛋白复合水凝胶的制备方法. CN: CN111995777A, 2020-11-27.
[6] 樊喜英, 刘文帅, 年锐, 孙粤, 包子娴, 咸漠. 一种用于蛋白沉淀的装置及从蛋白混合液中分离纯化目的蛋白的方法. CN: CN111471084A, 2020-07-31.
[7] 刘文帅, 樊喜英, 年锐, 孙粤, 包子娴, 咸漠. 一种纳米抗体分离纯化的方法. CN: CN111378030A, 2020-07-07.
[8] 包子娴, 孙粤, 年锐, 咸漠, 陈泉, 刘文帅, 彭馨莹, 樊喜英. 一种复用对流色谱系统及其用于纯化蛋白的方法. CN: CN111318077A, 2020-06-23.
[9] 包子娴, 孙粤, 年锐, 咸漠, 陈泉, 刘文帅, 彭馨莹, 樊喜英. 制备溶液渗透性目的蛋白沉淀物及从宿主细胞中分离纯化目的蛋白的方法. CN: CN111100183A, 2020-05-05.
[10] 包子娴, 年锐, 咸漠, 孙粤, 陈泉, 刘文帅, 彭馨莹. 一种高机械强度的蛛丝蛋白-胶原蛋白复合水凝胶的制备方法. CN: CN110684208A, 2020-01-14.
[11] 包子娴, 孙粤, 年锐, 咸漠, 陈泉, 刘文帅, 彭馨莹, 樊喜英. 一种复用对流色谱系统. CN: CN209362021U, 2019-09-10.
[12] 刘文帅, 年锐, 孙粤, 包子娴, 樊喜英, 咸漠. 一种蛋白分离纯化的方法. CN: CN109206476A, 2019-01-15.
[13] 孙粤, 年锐, 咸漠, 包子娴, 陈泉, 刘文帅, 樊喜英. 一种高质量可放大型鱼胶原蛋白提取纯化方法. CN: CN108642115A, 2018-10-12.

   

[1] Xiying Fan, Xinying Peng, Tingting Wang, Yi Gu, Guochuan Sun, Qinghui Shou, Haipeng Song, Rui Nian, Wenshuai Liu. Optimized silk fibroin nanoparticle functionalization with anti-CEA nanobody enhancing active targeting of colorectal cancer cells. Biomedical Materials[J]. 2023, [2] Xiying Fan, Tingting Wang, Min Han, Yi Gu, Guochuan Sun, Xinying Peng, Qinghui Shou, Haipeng Song, Wenshuai Liu, Rui Nian. Dual CEA/CD44 targeting to colorectal cancer cells using nanobody-conjugated hyaluronic acidmodified mesoporous silica nanoparticles with pH- and redox-sensitivity hyaluronic acidmodified mesoporous silica nanoparticles with pH- and redox-sensitivity. Materials Advances[J]. 2022, [3] Jianli Yu, Yang Guo, Yi Gu, Xiying Fan, Fei Li, Haipeng Song, Rui Nian, Wenshuai Liu. A novel silk fibroin protein–based fusion system for enhancing the expression of nanobodies in Escherichia coli. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY[J]. 2022, 1-11, [4] Jianli Yu, Yang Guo, Yi Gu, Fei Li, Haipeng Song, Rui Nian, Xiying Fan, Wenshuai Liu. Targeting and neutralizing human epididymis protein 4 by novel nanobodies to suppress ovarian cancer cells and attenuate cisplatin resistance. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES[J]. 2022, 199: 298-306, [5] Fan, Xiying, Liang, Yunlong, Li, Fei, Yu, Jianli, Song, Haipeng, Feng, Dongxiao, Xian, Mo, Liu, Wenshuai, Nian, Rui. Integrated purification of a nanobody using ammonium sulfate precipitation and Capto MMC. JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY[J]. 2020, 95(1): 246-254, https://www.webofscience.com/wos/woscc/full-record/WOS:000495333100001.
[6] Gu, Yi, Cao, Hui, Li, Fei, Yu, Jianli, Nian, Rui, Feng, Dongxiao, Lin, Jingtao, Song, Haipeng, Liu, Wenshuai. Production of functional human nerve growth factor from the submandibular glands of mice using a CRISPR/Cas9 genome editing system. WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY[J]. 2020, 36(12): http://dx.doi.org/10.1007/s11274-020-02951-x.
[7] Zhou, Dongyan, Sun, Yue, Bao, Zixian, Liu, Wenshuai, Xian, Mo, Nian, Rui, Xu, Fei. Improved Cell Viability and Biocompatibility of Bacterial Cellulose through in Situ Carboxymethylation. MACROMOLECULAR BIOSCIENCE[J]. 2019, 19(5): https://www.webofscience.com/wos/woscc/full-record/WOS:000471340300004.
[8] Chen, Quan, Zhou, Yuhang, Yu, Jianli, Liu, Wenshuai, Li, Fei, Xian, Mo, Nian, Rui, Song, Haipeng, Feng, Dongxiao. An efficient constitutive expression system for Anti-CEACAM5 nanobody production in the yeast Pichia pastoris. PROTEIN EXPRESSION AND PURIFICATION[J]. 2019, 155: 43-47, http://dx.doi.org/10.1016/j.pep.2018.11.001.
[9] Liu, Wenshuai, Fan, Xiying, Wang, Xingang, Bao, Zixian, Sun, Yue, Rai, Kamal, ShaliutinaKolesova, Anna, Su, Jianhua, Xian, Mo, Nian, Rui. Salt-enhanced permeabilization for monoclonal antibody precipitation and purification in a tubular reactor with a depth filtration membrane with advanced chromatin extraction. BIOCHEMICAL ENGINEERING JOURNAL[J]. 2019, 151: http://dx.doi.org/10.1016/j.bej.2019.107332.
[10] Wang, Zhenyu, Wang, Chengdong, Hou, Hongbin, Liu, Wenshuai, Nian, Rui, Sun, Hongguang, Chen, Xiao, Cui, Guanglei. A facile fabrication of stimulus-responsive amorphous photonic crystals in the near-infrared region. APPLIED SURFACE SCIENCE[J]. 2019, 479: 1014-1020, http://dx.doi.org/10.1016/j.apsusc.2019.02.143.
[11] Liu, Wenshuai, Zhou, Dongyan, Sun, Yue, Yu, Jianli, Chen, Quan, Bao, Zixian, Fan, Xiying, Liang, Yunlong, Peng, Xinying, Xian, Mo, Nian, Rui. Reduction of chromatin heteroaggregates by acid precipitation of mammalian cell culture and ramification in protein A chromatography for recombinant immunoglobulin G purification. BIOCHEMICAL ENGINEERING JOURNAL[J]. 2019, 143: 65-74, http://dx.doi.org/10.1016/j.bej.2018.12.012.
[12] Liu, Wenshuai, Sun, Yue, Yu, Jianli, Chen, Quan, Bao, Zixian, Fan, Xiying, Liang, Yunlong, Peng, Xinying, Xian, Mo, Nian, Rui. Advance chromatin extraction improves the performance of electropositive mixed-mode chromatography as a capture step and enables its integration with void-exclusion anion exchange chromatography as a two-column-step purification platform for monoclonal antibody production. BIOCHEMICAL ENGINEERING JOURNAL[J]. 2019, 142: 145-152, http://dx.doi.org/10.1016/j.bej.2018.11.018.
[13] Fan, Xiying, Zhou, Yuhang, Yu, Jianli, Li, Fei, Chen, Quan, Song, Haipeng, Feng, Dongxiao, Liu, Wenshuai, Nian, Rui, Xian, Mo. Non-affinity purification of a nanobody by void-exclusion anion exchange chromatography and multimodal weak cation exchange chromatography. SEPARATION AND PURIFICATION TECHNOLOGY[J]. 2019, 225: 88-96, http://dx.doi.org/10.1016/j.seppur.2019.05.068.
[14] Liu, Wenshuai, Fan, Xiying, Wang, Xingang, Rai, Kamal, Su, Jianhua, Xian, Mo, Nian, Rui. Chromatin-directed clarification in cell culture fluid enables non-protein affinity antibody purification by tangential flow filtration integrated with high-capacity cation exchange chromatography. BIOCHEMICAL ENGINEERING JOURNAL[J]. 2019, 151: http://dx.doi.org/10.1016/j.bej.2019.107315.
[15] Liu, Wenshuai, Chen, Quan, Fan, Xiying, Liang, Yunlong, Sun, Yue, Yu, Jianli, Bao, Zixian, Peng, Xinying, Xian, Mo, Nian, Rui. A seamless and nonprotein A-based monoclonal antibody purification platform using void-exclusion anion exchange chromatography and electropositive multimodal chromatography enhanced by advance chromatin extraction. JOURNAL OF CHEMICAL TECHNOLOGY AND BIOTECHNOLOGY[J]. 2019, 94(10): 3344-3355, [16] Liu, Wenshuai, Song, Haipeng, Chen, Quan, Yu, Jianli, Xian, Mo, Nian, Rui, Feng, Dongxiao. Recent advances in the selection and identification of antigen-specific nanobodies. MOLECULAR IMMUNOLOGY[J]. 2018, 96: 37-47, http://dx.doi.org/10.1016/j.molimm.2018.02.012.
[17] Liu, Wenshuai, Zhang, Qinling, Guo, Jia, Chen, Zhi, Li, Jilun, Wen, Ying. Increasing Avermectin Production in Streptomyces avermitilis by Manipulating the Expression of a Novel TetR-Family Regulator and Its Target Gene Product. APPLIED AND ENVIRONMENTAL MICROBIOLOGY[J]. 2015, 81(15): 5157-5173, https://www.webofscience.com/wos/woscc/full-record/WOS:000357668600030.

   

（ 1 ） 人附睾蛋白4（HE4）纳米抗体制备, 主持, 院级, 2021-04--2022-04