Post-harvest processing of sweetpoato

Fuel ethanol and butanol production

2009.06 Environmental sciences, Chengdu Institute of Biology, Chinese Academy of Sciences

Since 2016, researched on quality assessment of sweetpotato .

Since 2009, researched on butanol fermentation from wheat straw .

Since 2006, researched on fuel ethanol production from sweet potato.

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1. Ling Guo#, Yanling Jin#, Yao Xiao, Li Tan, Xueping Tian, Yanqiang Ding, Kaize He,Anping Du, Jinmeng Li, Zhuolin Yi, Songhu Wang, Yang Fang*, Hai Zhao*. Energy-efficient and environmentally friendly production of starch-rich duckweed biomass using nitrogen-limited cultivation. Journal of Cleaner Production, doi: https://doi.org/10.1016/j.jclepro.2019.119726

2. Barkat Ali#, Zhuolin Yi#, Yang Fang, Lanchai Chen, Kaize He, Dayu Liu, Huibo Luo, Dong Zhao, Hui He, Yanling Jin*, Hai Zhao*.Characterization of a fungal thermostable endoglucanase from Chinese Nong-flavor daqu by metatranscriptomic method .International Journal of Biological Macromolecules, 2019, 121:183-190

3. Ya-Liang Xu, Yang Fang, Qi Li, Gui-Li Yang, Ling Guo, Guo-Ke Chen, Li Tan, Kai-Ze He, Yan-ling Jin*, Hai Zhao*. Turion, an innovative duckweed-based starch production system for economical biofuel manufacture. Industrial Crops and Products, 2018(124): 108-114.

4. Zhuolin Yi, Yang Fang, Kaize He, Dayu Liu, Huibo Luo, Dong Zhao, Hui He, Yanling Jin*, Hai Zhao*.Directly mining a fungal thermostable α-amylase from Chinese Nong-flavor liquor starter.Microbial Cell Factories,2018 , 17 (1) :30

5. Yanling Jin, Yang Fang, Guohua Zhang, Lingling Zhou, Hai Zhao*. Comparison of ethanol production performance in ten varieties of sweet potato at different growth stages. Acta Oecologica,2012,44:33-37

6. Jin Y,Fang Y,Huang M,Sun J,Huang Y,Gao X,Li R,He K,Zhao H*. Combination of RNA sequencing and metabolite data to elucidate improved toxic compound tolerance and butanol fermentation of Clostridium acetobutylicum from wheat straw hydrolysate by supplying sodium sulfide. Bioresource Technology, 2015,198:77-86 

7. Qian Chen, Yang Fang, Hai Zhao, Guohua Zhang, Yanling Jin*. Transcriptional analysis of Saccharomyces cerevisiae during high-temperature fermentation. Annals Of Microbiology, 2013, 63（4）:1433–1440.

8. Xiaofeng Gao, Hai Zhao, Guohua Zhang, Kaize He,Yanling Jin*. Genome Shuffling of Clostridium acetobutylicum CICC 8012 for Improved Production of Acetone–Butanol–Ethanol (ABE). Current Microbiology, 2012, 65:128–132

9. Qing Zhang, Hai Zhao, Guohua Zhang, Kaize He, Zhirong Yang,Yanling Jin*. Transcriptome analysis of Saccharomyces cerevisiae at the late stage of very high gravity (VHG) fermentation.African Journal of Biotechnology,2012,11(40): 9641-9648 

10. Xinhui Wang, Yanling Jin*, Yang Fang, Yuhong Huang, Guohua Zhang,Xiaofeng Gao,Hai Zhao. The chloride ion responsible for filament formament and inhibibitory effect on cell division in zymomonas mobilis 232B. African journal of microbiology research. 2011，5(29)：5260-5265

11. Li Tan，Jia Zhong, Yan-Ling Jin, Zhao-Yong Sun⁎Yue-Qing Tang, Kenji Kida. Production of bioethanol from unwashed-pretreated rapeseed straw at high solid loading.Bioresource Technology,2020,303:122949

12. Guoke Chen, Jun Huang, Yang Fang*, Yonggui Zhao, Xueping Tian, Yanling Jin,Hai Zhao*.Microbial community succession and pollutants removal of a novel carriers enhanced duckweed treatment system for rural wastewater in Dianchi Lake basin.Bioresource Technology,2019,276:8-17

13. Ya-Liang Xu, Li Tan, Ling Guo, Gui-Li Yang, Qi Li, Fan Lai, Kai-Ze He,Yan-ling Jin, Anping Du, Yang Fang*, Hai Zhao*.Increasing starch productivity of Spirodela polyrhiza by precisely control the spectral composition and nutrients status.Industrial Crops & Products,2019,134:284-291.（Qing Zhang, Yan-Ling Jin, Yang Fang, Hai Zhao*. Adaptive evolution and selection of stress-resistant Saccharomyces cerevisiae for very high gravity bioethanol fermentation, Electronic Journal of Biotechnology, https://doi.org/10.1016/j.ejbt.2019.06.003

14. Zhuolin Yi, Yanling Jin, Yao Xiao, Lanchai Chen,  Li Tan, Anping Du, Kaize He,  Dayu Liu, Huibo Luo, Yang Fang*, Hai Zhao*. Unraveling the Contribution of High Temperature Stage to Jiang-Flavor Daqu, a Liquor Starter for Production of Chinese Jiang-Flavor Baijiu, With Special Reference to Metatranscriptomics. Front. Microbiol.，2019，10:472 | doi: 10.3389/fmicb.2019.00472.

15. Guoke Chen,Yang Fang,Jun Huang,  Yonggui Zhao,Qi Li, Fan Lai,Yaliang Xu,Xueping Tian,Kaize He,Yanling Jin, Li Tan and Hai Zhao*.Duckweed systems for eutrophic water purification through converting wastewater nutrients to high-starch biomass: comparative evaluation of three different genera (Spirodela polyrhiza, Lemna minor and Landoltia punctata) in monoculture or polyculture,Rsc Advances , 2018 , 8

16. Gui-Li Yang, Yang Fang, Ya-Liang Xu, Li Tan, Qi Li, Yang Liu, Fan Lai, Yan-Ling Jin, An-Ping Du, Kai-Ze He, Xin-Rong Ma*, Hai Zhao*. Frond transformation system mediated by Agrobacterium tumefaciens for Lemna minor. Plant Molecular Biology. 2018，98（4-5）:319-331.

17. Guo, Ling; Ding, Yanqiang; Xu, Yaliang; Li Zhidan; Jin Yanling; He Kaize; Zhao Hai*.Responses of Landoltia punctata to cobalt and nickel: Removal, growth, photosynthesis, antioxidant system and starch metabolism.Aquatic Toxicology, 2017,190: 87-939

18. Yuhong huang†, zhuolin yi†, yanling jin, yonggui zhao, Dong Zhao, hui he, dayu liu, Huibo Luo, Wenxue Zhang, Yang Fang*, and Hai Zhao*.New microbial resource: microbial diversity, function and dynamics in Chinese liquor starter. Scientific reports,2017 Nov 6;7(1):14577. doi: 10.1038/s41598-017-14968-8. 

19. Huang YH*, Yi ZL*, Jin YL, Huang MJ, He KZ, Liu DY, Luo HB, Zhao D, He H, Fang Y#, Zhao H#. Metatranscriptomics Reveals the Functions and Enzyme Profiles of the Microbial Community in Chinese Nong-Flavor Liquor Starter. Front Microbiol. 2017 Sep 12;8:1747. doi: 10.3389/fmicb.2017.01747.Yuhong Huang, William G Willats, Lene Lange, Yanling Jin, Yang Fang, Armando A Salmeán, Henriette L Pedersen, Peter Kamp Busk, Hai Zhao*.High-throughput microarray mapping of cell wall polymers in roots and tubers during the viscosity reducing process. Biotechnology and Applied Biochemstry，2016 ，63(2):178-89 

20. Shen, N; Wang, QY; Zhu, J; Qin, Y; Liao, SM; Li, Y; Zhu, QX; Jin, YL; Du, LQ; Huang, RB. Succinic acid production from duckweed (Landoltia punctata) hydrolysate by batch fermentation of Actinobacillus succinogenes GXAS137. Bioresource Technology , 2016 , 211 :307-312 Bioresource Technology, 2015,198:77-86

21. Huang†, MJ; Fang, Y†; Liu, Y; Jin, YL; Sun, JL; Tao, X; Ma, XR; He, KZ; Zhao, H*. Using proteomic analysis to investigate uniconazole-induced phytohormone variation and starch accumulation in duckweed (Landoltia punctata).BMC Biotechnology,2015,15:81-93

22. Yang Liu, Yang Fang, Mengjun Huang, Yanling Jin, Jiaolong Sun Xiang Tao, Guohua Zhang, Kaize He,Yun Zhao, Hai Zhao*.Uniconazole- 

 induced starch accumulation in the bioenergy crop duckweed (Landoltia punctata) I: transcriptome analysis of the effects of uniconazole on chlorophyll and endogenous hormones biosynthesis, Biotechnology for Biofuels, 2015, 8:57-63.

23. Yang Liu, Yang Fang, Mengjun Huang, Yanling Jin, Jiaolong Sun, Xiang Tao, Guohua Zhang, Kaize He, Yun Zhao, Hai Zhao*.Uniconazole-induced starch accumulation in the bioenergy crop duckweed (Landoltia punctata) II: transcriptome alterations of pathways involved in carbohydrate metabolism and endogenous hormones crosstalk, Biotechnology for Biofuels, 2015, 8:64-70 .

24. Yonggui Zhao, Yang Fang, Yanling Jin, Jun Huang, Xinrong Ma, Kaize He,Zhiming He, Feng Wang, Hai Zhao*.Microbial community and removal of nitrogen via the addition of a carrier in a pilot-scale duckweed-based wastewater treatment system. Bioresource Technology,2015,179:549-558

25. Lanchai Chen, Yang Fang, Yanling Jin, Yao Xiao, Yonggui Zhao, Hai Zhao*. Biosorption of Cd2+ by untreated dried powder of duckweed Lemna aequinoctialis. Desalination and Water Treatment. 2015,53(1): 183-194

26. Y. Zhao, Y. Fang, Y. Jin, J. Huang, S. Bao, T. Fu, Z. He, F. Wang, M. Wang & H. Zhao*. Pilot-scale comparison of four duckweed strains from different genera for potential application in nutrient recovery from wastewater and valuable biomass production. Plant Biology. 2015, 1:82-90.

27. Yonggui Zhao, Yang Fang, Yanling Jin, Jun Huang, Shu Bao, Zhiming He, Feng Wang, Hai Zhao*. Effects of operation parameters on nutrient removal from wastewater and high-protein biomass production in a duckweed-based (Lemma aequinoctialis) pilot-scale system. Water Science and Technology, 2014,70(7):1195-1204

28. Mengjun Huang, Yang Fang, Yao Xiao, Jiaolong Sun, Yanling Jin, Xiang Tao, Xinrong Ma, Kaize He, Hai Zhao*. Proteomic analysis to investigate the high starch accumulation of duckweed (Landoltia punctata) under nutrient starvation. Industrial Crops and Products, 2014,59: 299–308.

29. Yonggui Zhao, Yang Fang, Yanling Jin, Jun Huang, Shu Bao, Tian Fu, Zhiming He,Feng Wang, Hai Zhao*. Potential of duckweed in the conversion of wastewater nutrients to valuable biomass: A pilot-scale comparison with water hyacinth.Bioresource Technology，2014，163：82–91

30. Yuhong Huang, Yanling Jin, Weiliang Shen,Yang Fang, Guohua Zhang, Hai Zhao*. The use of plant cell wall-degrading enzymes from a newly isolated Penicillium ochrochloron Biourge for viscosity reduction in ethanol production with fresh sweet potato tubers as feedstock .Biotechnology and Applied Biochemistry, 2014, 61(4):480–491 

31. Yuhong Huang, Yanling Jin, Yang Fang, Yuhao Li, Hai Zhao*. Simultaneous utilization of non-starch polysaccharides and starch and viscosity reduction for bioethanol fermentation from fresh Canna edulis Ker. tubers. Bioresource Technology，2013，128：560–564.

32. Xiang Tao, Yang Fang, Yao Xiao, Yanling Jin, Xinrong Ma, Yun Zhao, Kaize He, Hai Zhao*,Haiyan Wang*. Comparative transcriptome analysis to investigate the high starch accumulation of duckweed ( Landoltia punctata) under nutrient starvation. Biotechnology for Biofuels, 2013, 6:72.

33. Yao Xiao, Yang Fang, Yanling Jin, Guohua Zhang, Hai Zhao*. Culturing duckweed in the field for starch accumulation. Industrial crops and products. Industrial Crops and Products, 2013，48：183– 190.

34. Yuhong Huang, Yanling Jin, Yang Fang, Yuhao Li, Guohua Zhang, Qian Chen, Hai Zhao*. Simultaneous saccharification and fermentation (SSF) of non-starch polysaccharides and starch from fresh tuber of Canna edulis ker at a high solid content for ethanol production. Biomass & bioenergy, 2013，52: 8-14.

35. Qian Chen, Yanling Jin, Guohua Zhang,Yang Fang,Yao Xiao,Hai Zhao*. Improving Production of Bioethanol from Duckweed (Landoltia punctata) by Pectinase Pretreatment.Energies,2012, 5, 3019-3032

36. Huiling Xue , Yao Xiao , Yanling Jin, Xinbo Li, Yang Fang, Hai Zhao*.Genetic diversity and geographic differentiation analysis of duckweed using inter-simple sequence repeat markers. Molecular Biology Reports, 2012,39（1）:547–554

37. Liang Zhang, Hai Zhao*, Mingzhe Gan, Yanling Jin , Xiaofeng Gao , Qian Chen,Jiafa Guan, Zhongyan Wang. Application of simultaneous saccharification and fermentation (SSF)from viscosity reducing of raw sweet potato for bioethanol production at laboratory, pilot and industrial scales.Bioresource Technology, 2011, 102（6）：4573–4579

38. Xinhui Wang, Yuhong Huang, Yang Fang, Yanling Jin, Guohua Zhang, Xiao feng Gao,Hai Zhao*.Respective effects of sodium and chloride ion on physiological property of Zymomonas mobilis 232B. African Journal of Biotechnology,2011,11(36):8880-8888

39. Liang Zhang, Qian Chen,Yanling Jin, Huilin Xue, Jiafa Guan, Zhongyan Wang, Hai Zhao*. Energy-saving direct ethanol production from viscosity reduction mash of sweet potato at very high gravity. Fuel Processing Technology,2010 , 1845-1850

40. 靳艳玲，沈维亮，丁凡，余金龙，方扬，谭力，易卓林，何开泽，赵海*.甘薯淀粉加工废渣生产蛋白饲料的技术.连城地瓜产业发展大会论文集[C].2019:157-168

41. 靳艳玲，杨林，丁凡，方扬，谭力，易卓林，何开泽，赵海.*不同品种甘薯淀粉加工特性及其与磷含量的相关性研究，食品工业科技，2019，13:46-51

42. 靳艳玲，谭力，杨林，丁彦强，何素兰，李育明，何开泽，赵海*.不同品种甘薯不同部位的抗氧化活性研究，食品研究与开发，2019，40（12）:51-58

43. Yanling Jin, Lin Yang,Yang Fang, Zhuolin Yi, Li Tan, Kaize He, Hai Zhao* .Investigation and evaluation of the agricultural chemicals and heavy metal residue in sweeetpotato[C]. 8^th international sweetpotato symposium.Korea,2018.9.5-8,35

44. 靳艳玲，方扬, 黄孟军, 孙蛟龙, 黄玉红, 郜晓峰,李仁强, 何开泽, 赵海*.硫化钠促进Clostridium acetobutylicum CICC8012 发酵麦秸水解液产丁醇的机制研究[D].2018中国可再生能源学术大会论文摘要集.北京，2018.8.21-23,299.

45. 靳艳玲，何素兰，李育明，沈维亮，方扬，谭力，易卓林，何开泽，赵海*.不同品种甘薯淀粉产量及糊化特性的比较研究. 江苏师范大学报（自然科学版），2018，36，1:17-20

46. 靳艳玲，丁凡，余金龙，沈维亮，方扬，谭力，易卓林，何开泽，赵海*.甘薯生化转化技术研究. 中国粮油学报，2017,32(9): 193-196

47. 靳艳玲 赵海 方扬.高粘度快速发酵生产燃料乙醇技术研究最终报告.科技创新导报，2016.1:170-171.

48. Yanling Jin, Yuhong Huang , Yang Fang, Weiliang Shen, Zhuolin Yi, Li Tan, Hai Zhao*. High-throughput microarray mapping of cell wall polymers in roots and tubers during the viscosity reducing process. Proceedings of 7th China-Japan-Korea International Sweetpotato Workshop,October 10-14,2016,Jinan,China,157-158.

49. Yanling Jin, Sulan He,Yuming Li, Yanqiang Ding, Zhuolin Yi,Yang Fang, Weiliang Shen, Hai Zhao*.Rhizosphere microbial communities of sweetpotato and their role in assisting sweetpotato to be a pioneer crop in the cultivation of virgin land. Proceedings of 7th China-Japan-Korea International Sweetpotato Workshop, October 10-14,2016,Jinan,China,94-95.

50. 靳艳玲，刘玉婷，沈维亮，方扬，赵海*.甘薯淀粉发酵废渣乳酸活性益生菌饲料发酵技术.甘薯青年学术讨论会摘要集，2015，成都，P77

51. Yan-Ling Jin, Yang Fang, Wei-Liang Shen, Guo-Hua Zhang, Hai Zhao*.Lactic acid production by Lactobacillus rhamnosus from sweet potato residue, New Era of Sweetpotato Research in East Asia,2014,Japan,P49-50

52. 靳艳玲，方扬，沈维亮，赵海*. 清洁高效甘薯淀粉加工技术体系，农业工程技术:农产品加工业，2014，4（4）：18-21

53. Jin Yan ling, Zhang Liang, Fang Yang, Zhao Hai *. Application of Enzyme Systems for Viscosity Reduction in Bioethanol  Production from Fresh Sweet Potato[C].Proceedings of China xuzhou international sweetpotato symposium (ISBN978-7-5655-0125-8). 2010, Xuzhou, P599-606

54. 靳艳玲，方扬，赵海*.高效甘薯燃料乙醇生产技术体系[C].第十二届中国科协年会非粮生物质能源与高技术产业化研讨会论文集，2010，福州，P39－41

55. 靳艳玲，郜晓峰，方扬，赵海*.鲜芭蕉芋全原料快速酒精发酵工艺初探[C].全国博士生学术会议，生物能源－地球母亲的绿色能源论文集,2010：127~133

56. Yanling Jin, Hai Zhao*.High Performance Technologies for Ethanol Production from Sweet Potato. International conference on biomass energy technologies(ICBT2010), August 21-23, 2010, Beijing, P257

57. 靳艳玲，甘明哲，方扬，赵海*. 鲜甘薯发酵生产高浓度乙醇的技[J].应用与环境生物学报，2009，15(3)410～413

58. 靳艳玲，甘明哲，周玲玲，赵海*. 4个品种甘薯不同生育期的乙醇发酵比较研究[J].应用与环境生物学报，2009,15（2）：267～269

59. Jin Yanling, Fang Yang, Zhao Hai*.Relative characteristics on ethanol production from sweet potato[C].Biomass and organic waste as sustainable resources international conference,2009:33 

60. 靳艳玲，方扬，赵海*.以保存甘薯和腐烂甘薯为原料发酵产燃料乙醇的研究.2009全国生物质能源大会会议论文集：160~166

61. Yanling Jin,Hai Zhao*.Relative Characteristics of Ethanol Production of Sweet Potato. Proceedings of 3rd China-Japan-Korea Workshop on Sweetpotato，Beijing: China Agricultural University Press,2008.427～4322

1. A method to improve the yield of duckweed starch by optimizing spectral composition. Cn 108323427b

2. A method to improve the total starch yield of duckweed by promoting the formation of dormancy body. Cn 107548982 b)

3. Drum type potato starch extractor and water-saving starch extractor. Zl201410500105.3

4. A rapid extraction method of anthocyanin from purple sweet potato. Zl201410317273.9

5. Methods to improve the yield of starches and crude protein of duckweed and the removal rate of nitrogen and phosphorus from sewage at the same time. ZL 201410198497.2

6. A method for preparing visbreaking enzyme from Mucor twiggy and its application. ZL 201310492417. X

7. A common Fusarium, a method of preparing visbreaking enzyme and its application. ZL 201310451257.4

8. A rapid method to increase the starch content of duckweed. Zl201310603781.9

9. Duckweed culture wave eliminator and its application in duckweed culture. ZL 201310704120.5

10 Drum type potato starch extractor and water-saving starch extractor. Zl201420557600.3

11. A strain with high yield and viscosity reducing enzyme system and its application. Zl201210234738.5

12. A method for the fermentation of acetone, ethanol and butanol from straw. Zl201210259521x

13. A method for the fermentation of acetone, ethanol and butanol from straw. Zl2012102600152

14. A method of producing fuel ethanol by duckweed fermentation. Zl201110169414.3

15. A method for the efficient production of fuel butanol from duckweed. Zl201110122135.1

16. A method for producing butanol by fermentation with cellulose as raw material. Zl201010213076.4

17. A quick method to reduce the viscosity of potato. Zl201110165042.7

18. A method for the determination of total sugar content in potato. Zl201110213410.0

19. A method for preservation of industrial sweet potato. Invention patent : ZL200610021316.4

20. A method for very high gravity ethanol fermentation. Invention patent :200610022209.3.

21.  A  method for rapid ethanol fermentation. Invention patent :200710049077.8.

22.A method for rapid ethanol fermentation by zymomonas mobilis. Invention patent :201010237753.6

1 Post-harvest processing from sweet potato;

2  Bio-liquid fuel production and environment protection

已指导学生

刘国强  硕士研究生  085238-生物工程  

李星  硕士研究生  085238-生物工程  

现指导学生

吴冬梅  硕士研究生  086000-生物与医药  

高雨  硕士研究生  086000-生物与医药