基本信息
谢海侠  女  博导  中国科学院水生生物研究所
电子邮件: xieh@ihb.ac.cn
通信地址: 武汉市武昌区东湖南路7号
邮政编码: 430072

研究领域

​病原细菌与宿主的相互作用;毒力岛型分泌系统的分子致病机理,以及毒力岛型分泌系统的杀菌机理


招生信息

   
招生专业
071004-水生生物学
071005-微生物学
090801-水产养殖
招生方向
病原微生物学
病原细菌与宿主互作

教育背景

2000-09--2005-07   中科院水生生物研究所   理学博士
1995-09--1999-07   华中农业大学   农学学士
学历
研究生 

学位
博士

工作经历

   
工作简历
2019-01~现在, 中国科学院水生生物研究所, 研究员
2011-01~2019-01,中科院水生生物研究所, 副研究员
2010-03~2010-08,帝国理工学院, 访问学者
2008-09~2010-12,中科院水生生物研究所, 助理研究员
2005-07~2008-01,新加坡国立大学, 博士后
1999-10~2005-07,中科院水生生物研究所, 研习员

专利与奖励

   
专利成果
[1] 谢海侠, 刘莹莉, 贺甜甜, 聂品, 韩冬. 一种预防黄颡鱼“裂头病”浸泡疫苗株的构建和应用. CN: CN112481185A, 2021-03-12.
[2] 昌鸣先, 武小曼, 谢海侠, 李楠, 聂品. 一种抗菌蛋白及分离的核酸、抗菌药物和应用. 中国: CN107540735A, 2018-01-05.

出版信息

   
发表论文
[1] 张淑雅, 李端友, 刘莹莉, 贺甜甜, 聂品, 谢海侠. 一种利用λ Red重组技术在杀鱼爱德华氏菌基因组添加标签的方法. 水生生物学报[J]. 2023, 47(6): 895-902, https://doi.org/10.7541/2023.2022.0461.
[2] Zeng, Zhi Xiong, Liu, Lu Yi, Xiao, Shui Bing, Lu, Jin Fang, Liu, Ying Li, Li, Jing, Zhou, Yuan Ze, Liao, Li Jing, Li, Duan You, Zhou, Ying, Nie, Pin, Xie, Hai Xia. Secreted in a Type III Secretion System-Dependent Manner, EsaH and EscE Are the Cochaperones of the T3SS Needle Protein EsaG of Edwardsiella piscicida. MBIO[J]. 2022, 13(4): http://dx.doi.org/10.1128/mbio.01250-22.
[3] Ying Li Liu, Tian Tian He, Xiu Long Jiang, Shan Shan Sun, Long Kun Wang, Pin Nie, Hai Xia Xie. Development of a hyper-adhesive and attenuated Edwardsiella ictaluri strain as a novel immersion vaccine candidate in yellow catfish (Pelteobagrus fulvidraco). MICROBIAL PATHOGENESIS[J]. 2022, 167: [4] Long Kun Wang, Shan Shan Sun, Shu Ya Zhang, Pin Nie, Hai Xia Xie. Orf1B controls secretion of T3SS proteins and contributes to Edwardsiella piscicida adhesion to epithelial cells. Veterinary Research[J]. 2022, [5] Li, Jia, McLaughlin, Richard William, Liu, Yingli, Zhou, Junying, Hu, Xueying, Wan, Xiaoling, Xie, Haixia, Hao, Yujiang, Zheng, Jinsong. Biofilm formation, antimicrobial assay, and toxin-genotypes of Clostridium perfringens type C isolates cultured from a neonatal Yangtze finless porpoise. ARCHIVES OF MICROBIOLOGY[J]. 2022, 204(7): http://dx.doi.org/10.1007/s00203-022-02990-y.
[6] Li, Duan You, Liu, Ying Li, Liao, Xiao Jian, He, Tian Tian, Sun, Shan Shan, Nie, Pin, Xie, Hai Xia. Identification and Characterization of EvpQ, a Novel T6SS Effector Encoded on a Mobile Genetic Element in Edwardsiella piscicida. FRONTIERS IN MICROBIOLOGY[J]. 2021, 12: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7991086/.
[7] He, Tian Tian, Zhou, Ying, Liu, Ying Li, Li, Duan You, Nie, Pin, Li, Ai Hua, Xie, Hai Xia. Edwardsiella piscicida type III protein EseJ suppresses apoptosis through down regulating type 1 fimbriae, which stimulate the cleavage of caspase-8. CELLULAR MICROBIOLOGY[J]. 2020, 22(7): https://www.webofscience.com/wos/woscc/full-record/WOS:000540798600006.
[8] Wang, Ping, Jie, Li, He, Tian Tian, Li, Nan, Mo, Zhao Lan, Nie, Pin, Xie, Hai Xia. Pathogenic characterization ofAeromonas salmonicidasubsp.masoucidaturbot isolate from China. JOURNAL OF FISH DISEASES[J]. 2020, 43(10): 1145-1154, http://dx.doi.org/10.1111/jfd.13224.
[9] Zhang, Chang, Zhang, Qin, Wang, Junya, Tian, Jiayin, Song, Yunjie, Xie, Haixia, Chang, Mingxian, Nie, Pin, Gao, Qian, Zou, Jun. Transcriptomic responses of S100 family to bacterial and viral infection in zebrafish. FISH & SHELLFISH IMMUNOLOGY[J]. 2019, 94: 685-696, http://dx.doi.org/10.1016/j.fsi.2019.09.051.
[10] Jiang, Xinyu, Gao, Jingduo, Xue, Yujie, Qin, Yuting, Li, Xia, Sun, Zhaosheng, Xie, Haixia, Chang, Mingxian, Nie, Pin, Zou, Jun, Gao, Qian. Identification and expression analysis of IL-4/13 receptors in grass carp Ctenopharyngodon idella. FISH & SHELLFISH IMMUNOLOGY[J]. 2019, 87: 254-264, http://dx.doi.org/10.1016/j.fsi.2019.01.009.
[11] Qian Zhang, Tian Tian He, Duan You Li, Lu Yi Liu, Pin Nie, Haixia Xie. The Edwardsiella piscicida type III effector EseJ suppresses the expression of type 1 fimbriae leading to decreased bacterial adherence to host cells.. Infection & Immunity[J]. 2019, [12] Zhang, Qian, He, Tian Tian, Li, Duan You, Liu, Lu Yi, Nie, Pin, Xie, Hai Xia. The Edwardsiella piscicida Type III Effector EseJ Suppresses Expression of Type 1 Fimbriae, Leading to Decreased Bacterial Adherence to Host Cells. INFECTION AND IMMUNITY[J]. 2019, 87(7): [13] Edwards, Melissa S, McLaughlin, Richard William, Li, Jia, Wan, XiaoLing, Liu, Yingli, Xie, HaiXia, Hao, YuJiang, Zheng, JinSong. Putative virulence factors of Plesiomonas shigelloides. ANTONIE VAN LEEUWENHOEK INTERNATIONAL JOURNAL OF GENERAL AND MOLECULAR MICROBIOLOGY[J]. 2019, 112(12): 1815-1826, http://dx.doi.org/10.1007/s10482-019-01303-6.
[14] Gao, Jingduo, Jiang, Xinyu, Wang, Junya, Xue, Yujie, Li, Xia, Sun, Zhaosheng, Xie, Haixia, Nie, Pin, Zou, Jun, Gao, Qian. Phylogeny and expression modulation of interleukin 1 receptors in grass carp (Ctenopharyngodon idella). DEVELOPMENTAL AND COMPARATIVE IMMUNOLOGY[J]. 2019, 99(1): 103401-, http://dx.doi.org/10.1016/j.dci.2019.103401.
[15] Liu, Ying Li, He, Tian Tian, Liu, Lu Yi, Yi, Jia, Nie, Pin, Yu, Hong Bing, Xie, Hai Xia. The Edwardsiella piscicida Type III Translocon Protein EseC Inhibits Biofilm Formation by Sequestering EseE. APPLIED AND ENVIRONMENTAL MICROBIOLOGY[J]. 2019, 85(8): https://www.webofscience.com/wos/woscc/full-record/WOS:000463407200006.
[16] Wang, Gai Ling, Wang, Ming Cheng, Liu, Ying Li, Zhang, Qian, Li, Chuan Feng, Liu, Pan Ting, Li, En Zhong, Nie, Pin, Xie, Hai Xia. Identification, expression analysis, and antibacterial activity of NK-lysin from common carp Cyprinus carpio. FISH & SHELLFISH IMMUNOLOGY[J]. 2018, 73(1): 11-21, http://dx.doi.org/10.1016/j.fsi.2017.11.030.
[17] Wang, Gai Ling, Wang, Ming Cheng, Zhang, Xiao Wen, Chang, Ming Xian, Xie, Hai Xia, Nie, Pin. Molecular cloning, biological effect, and tissue distribution of interleukin-8 protein in mandarin fish (Siniperca chuasti) upon Flavobacterium columnare infection. FISH & SHELLFISH IMMUNOLOGY[J]. 2017, 66(1): 112-119, http://dx.doi.org/10.1016/j.fsi.2017.05.016.
[18] Wang Gai Ling, Wang Ming Cheng, Zhang Xiao Wen, Chang Ming Xian, Xie Hai Xia, Nie Pin. Molecular cloning, biological effect, and tissue distribution of interleukin-8 protein in mandarin fish ( Siniperca chuasti ) upon Flavobacterium columnare infection. FISH AND SHELLFISH IMMUNOLOGY[J]. 2017, [19] Yi, Lu, Nie, Pin, Yu, Hong Bing, Xie, Hai Xia. Regulation of Type III Secretion of Translocon and Effector Proteins by the EsaB/EsaL/EsaM Complex in Edwardsiella tarda. INFECTION AND IMMUNITY[J]. 2017, 85(9): https://www.webofscience.com/wos/woscc/full-record/WOS:000412696300017.
[20] Zhou, Ying, Liu, Lu Yi, He, Tian Tian, Laghari, Zubair Ahmed, Nie, Pin, Gao, Qian, Xie, Hai Xia. Edwardsiella tarda EsaE (Orf19 protein) is required for the secretion of type III substrates, and pathogenesis in fish. VETERINARY MICROBIOLOGY[J]. 2016, 190: 12-18, http://dx.doi.org/10.1016/j.vetmic.2016.05.003.
[21] 魏朝辉, 丁桂珍, 张立强, 艾桃山, 贺甜甜, 谢海侠. 嗜水气单胞菌asd-1基因功能研究. 湖北农业科学[J]. 2016, 55(10): 2667-2670, http://lib.cqvip.com/Qikan/Article/Detail?id=669444989.
[22] Yi, Jia, Xiao, Shui Bing, Zeng, Zhi Xiong, Lu, Jin Fang, Liu, Lu Yi, Laghari, Zubair Ahmed, Nie, Pin, Yu, Hong Bing, Xie, Hai Xia. EseE of Edwardsiella tarda Augments Secretion of Translocon Protein EseC and Expression of the escC-eseE Operon. INFECTION AND IMMUNITY[J]. 2016, 84(8): 2336-2344, https://www.webofscience.com/wos/woscc/full-record/WOS:000380746400017.
[23] Jia Yi, Shui Bing Xiao, Zhi Xiong Zeng, Jin Fang Lu, Lu Yi Liu, Zubair Ahmed Laghari, Pin Nie, Hong Bing Yu, Haixia Xie. EseE of Edwardsiella tarda augments secretion of translocon protein EseC and expression of escC~eseE operon.. Infection & Immunity[J]. 2016, [24] Lu, Jin Fang, Wang, Wei Na, Wang, Gai Ling, Zhang, He, Zhou, Ying, Gao, Zhi Peng, Nie, Pin, Xie, Hai Xia. Edwardsiella tarda EscE (Orf13 Protein) Is a Type III Secretion System-Secreted Protein That Is Required for the Injection of Effectors, Secretion of Translocators, and Pathogenesis in Fish. INFECTION AND IMMUNITY[J]. 2016, 84(1): 2-10, http://www.irgrid.ac.cn/handle/1471x/1012344.
[25] Xie, HaiXia, Lu, JinFang, Zhou, Ying, Yi, Jia, Yu, XiuJun, Leung, Ka Yin, Nie, Pin. Identification and Functional Characterization of the Novel Edwardsiella tarda Effector EseJ. INFECTION AND IMMUNITY[J]. 2015, 83(4): 1650-1660, http://www.irgrid.ac.cn/handle/1471x/978714.
[26] Li, Nan, Qin, Ting, Zhang, Xiao Lin, Huang, Bei, Liu, Zhi Xin, Xie, Hai Xia, Zhang, Jin, McBride, Mark J, Nie, Pin. Gene Deletion Strategy To Examine the Involvement of the Two Chondroitin Lyases in Flavobacterium columnare Virulence. APPLIED AND ENVIRONMENTAL MICROBIOLOGY[J]. 2015, 81(21): 7394-7402, http://www.irgrid.ac.cn/handle/1471x/994838.
[27] Gao, Zhi Peng, Nie, Pin, Lu, Jin Fang, Liu, Lu Yi, Xiao, Tiao Yi, Liu, Wei, Liu, Jia Shou, Xie, Hai Xia. Type III Secretion System Translocon Component EseB Forms Filaments on and Mediates Autoaggregation of and Biofilm Formation by Edwardsiella tarda. APPLIED AND ENVIRONMENTAL MICROBIOLOGY[J]. 2015, 81(17): 6078-6087, http://www.irgrid.ac.cn/handle/1471x/981954.
[28] Hai Xia Xie, Jin Fang Lu, Ying Zhou, Jia Yi, Xiu Jun Yu, Ka Yin Leung, Pin Nie. Idendification and functional characterization of the novel Edwardsiella tarda effector EseJ. INFECTION & IMMUNITY[J]. 2015, [29] Xie, HaiXia, Lu, JinFang, Rolhion, Nathalie, Holden, David W, Nie, Pin, Zhou, Ying, Yu, XiuJun. Edwardsiella tarda-Induced Cytotoxicity Depends on Its Type III Secretion System and Flagellin. INFECTION AND IMMUNITY[J]. 2014, 82(8): 3436-3445, http://dx.doi.org/10.1128/IAI.01065-13.
[30] Zhao, Li Juan, Lu, Jin Fang, Nie, P, Li, Ai Hua, Xiong, Bang Xi, Xie, Hai Xia. Roles of plasmid-encoded proteins, EseH, EseI and EscD in invasion, replication and virulence of Edwardsiella ictaluri. VETERINARY MICROBIOLOGY[J]. 2013, 166(1-2): 233-241, http://dx.doi.org/10.1016/j.vetmic.2013.05.023.
[31] Wang, Gailing, Li, Junhua, Zou, Pengfei, Xie, Haixia, Huang, Bei, Nie, Pin, Chang, Mingxian. Expression pattern, promoter activity and bactericidal property of beta-defensin from the mandarin fish Siniperca chuatsi. FISH & SHELLFISH IMMUNOLOGY[J]. 2012, 33(3): 522-531, http://dx.doi.org/10.1016/j.fsi.2012.06.003.
[32] Gailing Wang, Junhua Li, Pengfei Zou, Haixia Xie, Bei Huang, Pin Nie, Mingxian Chang. Expression pattern, promoter activity and bactericidal property of β-defensin from the mandarin fish Siniperca chuatsi. FISH AND SHELLFISH IMMUNOLOGY. 2012, 33(3): 522-531, http://dx.doi.org/10.1016/j.fsi.2012.06.003.
[33] 王良发, 谢海侠, 张金, 李楠, 姚卫建, 张立强, 熊传喜, 聂品. 我国淡水鱼类柱形病病原菌柱状黄杆菌的遗传多样性. 水生生物学报[J]. 2010, 367-377, http://lib.cqvip.com/Qikan/Article/Detail?id=33426566.
[34] Xie, Hai Xia, Yu, Hong Bing, Zheng, Jun, Nie, Pin, Foster, Leonard J, Mok, YuKeung, Finlay, B Brett, Leung, Ka Yin. EseG, an Effector of the Type III Secretion System of Edwardsiella tarda, Triggers Microtubule Destabilization. INFECTION AND IMMUNITY[J]. 2010, 78(12): 5011-5021, http://dx.doi.org/10.1128/IAI.00152-10.
[35] 张立强, 谢海侠, 李楠, 聂品. 柱状黄杆菌胶原蛋白酶的克隆与表达. 中国水产科学[J]. 2009, 786-790, http://lib.cqvip.com/Qikan/Article/Detail?id=31724148.
[36] Tian, J Y, Xie, H X, Zhang, Y A, Xu, Z, Yao, W J, Nie, P. Ontogeny of IgM-producing cells in the mandarin fish Siniperca chuatsi identified by in situ hybridisation. VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY[J]. 2009, 132(2-4): 146-152, http://dx.doi.org/10.1016/j.vetimm.2009.05.018.
[37] 刘毅, 谢海侠, 昌鸣先, 隗黎丽, 姚卫建, 聂品. 柱状黄杆菌胞外多糖对草鱼的免疫促进作用. 水产学报[J]. 2006, 30(5): 683-689, http://ir.ihb.ac.cn/handle/152342/1380.
[38] 田敬云, 谢海侠, 姚卫建, 聂品. 鳜鱼头肾的组织发生及成鱼头肾B淋巴细胞的分布. 动物学报[J]. 2005, http://ir.ihb.ac.cn/handle/152342/1796.
[39] 谢海侠. 柱状黄杆菌毒力相关因子的研究. 2005, 168-, http://www.irgrid.ac.cn/handle/1471x/263561.
[40] Xie Hai-Xia, Gao Qian, Nie Pin. Intestinal pathology of the mandarin fish Siniperca chuatsi infected naturally with the acanthocephalan Hebesomea violentum. ACTA HYDROBIOLOGICA SINICA[J]. 2005, 29(2): 137-141, http://www.irgrid.ac.cn/handle/1471x/543583.
[41] Tian Jing-Yun, Xie Hai-Xia, Yao Wei-Jian, Nie Pin. Ontogenetic development of the pronephros and the distribution of B-lymphocytes in adult pronephros of the mandarin fish Siniperca chuatsi. ACTA ZOOLOGICA SINICA[J]. 2005, 51(3): 440-446, http://www.irgrid.ac.cn/handle/1471x/543570.
[42] 谢海侠, 高谦, 聂品. 强壮粗体虫寄生引起的鳜肠道病理. 水生生物学报[J]. 2005, http://lib.cqvip.com/Qikan/Article/Detail?id=15161694.
[43] Xie HaiXia, Nie Pin, Wang Guitang. Genetic vaccines for prevention of fish diseases: Perspectives and prospects. ACTA HYDROBIOLOGICA SINICA[J]. 2004, 28(1): 90-95, http://ir.ihb.ac.cn/handle/342005/18167.
[44] 谢海侠, 聂品, 王桂堂. 鱼用核酸疫苗研究进展. 水生生物学报[J]. 2004, http://lib.cqvip.com/Qikan/Article/Detail?id=9017561.
[45] 谢海侠, 聂品. 鱼类胸腺研究进展. 水产学报[J]. 2003, 27(1): 90-96, http://lib.cqvip.com/Qikan/Article/Detail?id=7497663.
[46] 谢海侠. 寄生鳜鱼肠道的强壮粗体虫的形态、病理及免疫原性的初步研究. 中国动物学会第八次全国寄生虫学学术讨论会论文摘要汇编null. 2002, http://ir.ihb.ac.cn/handle/342005/15108.
[47] 谢海侠. 鳜鱼消化道内寄生强壮粗体虫的形态学及致病机理的初步观察. 鱼类病害研究[J]. 2001, 23(2): 7-10, http://lib.cqvip.com/Qikan/Article/Detail?id=5288017.

科研活动

   
科研项目
( 1 ) 鱼类烂鳃病病原的致病机理与免疫防治的基础研究, 参与, 国家级, 2009-01--2013-12
( 2 ) 鲶爱德华氏菌多拷贝质粒pEI1 和pEI2编码的毒力蛋白的分子致病机理研究, 主持, 国家级, 2012-01--2015-12
( 3 ) 杀鱼爱德华氏菌Ⅲ型分泌系统输送器蛋白的分子致病机理研究, 主持, 国家级, 2016-01--2019-12
( 4 ) 鲶爱德华氏菌Ⅲ型与Ⅵ型分泌系统致病的分子机制研究, 主持, 国家级, 2010-01--2012-12
( 5 ) 鱼类病原与宿主的相互作用和病原免疫逃逸新机制研究, 主持, 国家级, 2019-01--2022-12
( 6 ) 杀鱼爱德华氏菌EseJ蛋白调控I型菌毛和宿主细胞凋亡的研究, 主持, 国家级, 2021-01--2024-12

指导学生

已指导学生

易佳  硕士研究生  085238-生物工程  

周瑛  硕士研究生  071004-水生生物学  

张倩  硕士研究生  071004-水生生物学  

刘莹莉  硕士研究生  085238-生物工程  

李端友  硕士研究生  071004-水生生物学  

现指导学生

江修龙  硕士研究生  071004-水生生物学  

孙珊珊  博士研究生  071004-水生生物学  

汤璞玉  硕士研究生  071004-水生生物学  

张淑雅  博士研究生  071004-水生生物学