基本信息
孙飞  男  博导  中国科学院生物物理研究所
电子邮件: feisun@ibp.ac.cn
通信地址: 北京朝阳区大屯路15号8212室
邮政编码: 100101

研究领域

综合运用冷冻电镜三维重构、X射线晶体学以及其他生物物理等研究技术和手段,开展生物大分子复合体和膜蛋白的结构与功能研究,研究内容主要集中在冷冻电镜三维重构技术方法学、重要生物超大分子复合体和膜蛋白的结构功能研究以及生物膜(如线粒体膜)动态的分子机制研究三个方向。同时,瞄准冷冻电镜三维重构技术的前沿,深入开展新技术、新方法的研究。

招生信息

   
招生专业

071010-生物化学与分子生物学

招生方向
线粒体动态的分子机制;生物超大分子复合体结构功能;
膜蛋白、蛋白质复合体、细胞/细胞器的结构与功能研究;
生物成像方法学研究。 

教育背景

2001-09--2006-07   清华大学   博士
1997-09--2001-07   南京大学   学士
学历
清华大学 -- 研究生
学位
清华大学 -- 博士

工作经历

   
工作简历
2006/7 - 至今,中国科学院生物物理研究所,研究员
2012/7 - 至今,中国科学院生物物理研究所,研究平台生物成像中心,主任
社会兼职
2009年至今,中国生物物理学会理事,生物超微结构显微成像专业委员会副主任,青年工作委员会主任
2010年至今,中国电子显微镜学会低温电子显微学专业委员会委员
2012年至今,北京电镜学会理事
2010 - 2014,《生物物理学报》常务编委
2015年至今,Biophysics Reports, Associate Editor

教授课程

细胞成像技术
生物大分子电子显微三维重构
结构生物学导论
生物物理学系列讲座
生物大分子电子显微分析
国家税收
中国科学院大学研究生课程
《生物大分子电子显微三维重构》(专业选修课)
《细胞成像原理和技术》
华中科技大学-生物物理所贝时璋英才班课程
《生物成像原理和技术》
中国科学院大学本科生课程

《生物物理学概论》

专利与奖励

   
奖励信息
(1) 贝时璋青年生物物理学家奖, , 其他, 2009
(2) 全国优秀博士论文奖, , 国家级, 2008
(3) 中央国家机关优秀青年, , 国家级, 2008
专利成果
(1) 季刚,李硕果,孙飞;专利名称:光镜电镜关联成像用光学真空冷台,专利授权号:2014 2 0419041.X
(2)季刚,李硕果,孙飞;专利名称:一种光镜电镜关联成像用光学真空冷台;专利申请号:201410363314.8
(3)孙飞,庞效云,翟宇佳,王刚刚,谢天;专利名称:用于在细菌表面呈递目的蛋白的DNA分子及其应用;专利申请号:201310750736.6
(4)黄小俊,季刚,孙飞;专利名称:透射电子显微镜载网制备方法;专利申请号:201510355339.8

发表论文

     在电镜图像处理方法学研究、生物膜变形分子机制研究、生物超大分子复合体和膜蛋白/膜结合蛋白的结构功能研究领域均取得突出成果,在国际国内重要学术刊物上发表成果性研究论文96篇,包括以第一作者或通讯作者在Cell,Developmental Cell,Nature Communications,Nat. Struct. Mol. Biol.,PLoS Pathogens,STRUCTURE等国际知名学术刊物上发表的论文。
      论文目录如下:

 

1.       Wang S., Li S., Ji G., Huang X. and Sun F.* (2017), Using integrated correlative cryo-light and electron microscopy to directly observe syntaphilin-immobilized neuronal mitochondria in situ. Biophysics Reports (on line). doi: 10.1007/s41048-017-0035-x

2.       Chen Y., Wang Z., Zhang J., Li L., Wan X., Sun F.*, and Zhang F.* (2017), Accelerating electron tomography reconstruction algorithm ICON with GPU. Biophysics Reports (in press).

3.       Zhou Y., Lou H., Liu Z., Yang M., Pang X., Sun F. and Wang G.* (2017), Structural insight into the specific DNA template binding to DnaG primase in Bacteria. Scientific Report, 7(1): 659. doi: 10.1038/s41598-017-00767-8.

4.       Chan K.C., Lu L., Sun F., Fan J. (2017), Molecular details of the PH domain of ACAP1BAR-PH protein binding to PIP-containing membrane. J Phys Chem B. doi:10.1021/acs.jpcb.6b09563. [Epub ahead of print]

5.       Chen R., Gao B., Liu, X., Ruan F., Zhang Y., Lou J., Feng K., Wunsch C., Li S.M., Dai J.* and Sun F.* (2017), Molecular insights into the enzyme promiscuity of an aromatic prenyltransferase. Nature Chemical Biology (Advanced online at Dec. 19, 2016), 13(2): 226-234. doi: 10.1038/nchembio.2263.

6.      Wei R., Wang X., Zhang Y., Mukherjee S., Zhang L., Chen Q., Huang X., Jing S., Liu C., Li S., Wang G., Xu Y., Zhu S., Williams A., Sun F.* and Yin C.C.* (2016), Structural insights into Ca2+ -activated long-range allosteric channel gating of RyR1. Cell Research 26: 977-994 (Cover story). doi: 10.1038/cr.2016.99

7.       Wang S., Zhai Y., Pang X., Niu T., Ding Y.H., Dong, M.Q., Hsu W.V. Sun Z.* and Sun F.*(2016), Structural characterization of coatomer in its cytosolic state. Proten Cell 7(8): 586-600. doi: 10.1007/s13238-016-0296-z. [This research has been recommended In F1000Prime, 12 Dec 2016; DOI: 10.3410/f.726587512.793525970. F1000Prime.com/726587512#eval793525970]

8.       Chen Y., Zhang Y., Zhang K.*, Deng Y., Zhang F.* and Sun F.* (2016), FIRT: filtered iterative reconstruction technique with information restoration. Journal of Structural Biology 195(1) : 49-61. doi: 10.1016/j.jsb.2016.04.015.

9.       Deng Y., Chen Y., Zhang Y., Wang S., Zhang F.* and Sun F.* (2016), ICON: 3D reconstruction with ‘missing-information’ restoration in biological electron tomography. Journal of Structural Biology 195(1) : 100-112. doi: 10.1016/j.jsb.2016.04.004.

10.   Zhang J., Ji G., Huang X., Xu W.* and Sun F.* (2016), An improved cryo-FIB method for fabrication of frozen hydrated lamella. Journal of Structural Biology194(2): 218-223. doi: 10.1016/j.jsb.2016.02.013.

11.   Li W., Ding W., Ji G., Zhang J. and Sun F.* (2016), Three-dimensional visualization of arsenic stimulated mouse liver sinusoidal by FIB-SEM approach. Proten Cell 7(3) : 227-232. doi: 1007/s13238-016-0246-9.

12.   Shan H., Wang Z., Zhang F., Xiong Y., Yin C.C.* and Sun F.* (2016), A local-optimization refinement algorithm in single particle analysis for macromolecular complex with multiple rigid modules. Proten Cell 7(1): 46-62. doi:10.1007/s13238-015-0229-2.

13.   Mao G., Zhao Y., Kang X., Li Z., Zhang Y., Wang X., Sun F., Sankaran, K., and Zhang, X.C. (2016), Crystal structure of E. coli lipoprotein diacylglyceryl transferase. Nature communications 7, 10198.

14.   Cui Y., Zhao S., Wang J., Gao B., Fan Q., Sun F. and Zhou B. (2015), A novel mitochondrial carrier protein Mme1 acts as a yeast mitochondrial magnesium exporter. Biochim Biophys Acta. 1854(3): 724-32. doi:10.1016/j.bbamcr.2014.12.029.

15.   Ma D.K., Li Z., Lu A.Y., Sun F., Chen S., Rothe M., Menzel R., Sun F. and Horvitz HR.* (2015), Acyl-CoA Dehydrogenase Drives Heat Adaptation by Sequestering Fatty Acids. Cell, 161(5) : 1152-63. doi: 10.1016/j.cell.2015.04.026.

16.   Gao B., Chen R., Liu X., Dai J. and Sun F.* (2015), Expression, purification, crystallization and crystallographic study of the Aspergillus terreus acromatic prenyltransferase AtaPT. Acta Crystallogr F Struct Biol Commun. F71: 889-894. doi: 10.1107/S2053230X15008882.

17.   Sun F. (Sun Fang), Pang X., Xie T., Zhai Y., Wang G.G.* and Sun F.*  (2015), BrkAutoDisplay: functional display of multiple exogenous proteins on the surface of Escherichia coli by using BrkA autotransporter. Microbial Cell Factories. 14: 129. doi: 10.1186/s12934-015-0316-3.

18.   Liu B., Xue Y., Zhao W., Chen Y., Fan C., Gu L., Zhang Y., Sun L., Huang X., Ding W., Sun F., Ji W.* and Xu T.* (2015), Three-dimensional super-resolution protein localization correlated with vitrified cellular context. Sci Rep5, 13017. doi: 10.1038/srep13017.

19.   Han R., Wang L., Liu Z., Sun F.* and Zhang F.* (2015), A novel fully automatic scheme for fiducial marker-based alignment in electron tomography. Journal of Structural Biology 192: 403-17. doi: 10.1016/j.jsb.2015.09.022.  (The program markerauto can be downloaded from http://ear.ict.ac.cn/)

20.   Pang X., Fan J., Zhang Y., Zhang K., Gao B., Ma J., Li J., Deng Y., Zhou Q., Egelman E.H., Hsu V.W.* and Sun F.* (2014), A PH Domain in ACAP1 Possesses Key Features of the BAR Domain in Promoting Membrane Curvature. Developmental Cell, 31(1) : 73-86. doi: 10.1016/j.devcel.2014.08.020. [CAS News] This paper was commented and previewed in the same issue by Haucke and  colleagues (Dev. Cell. 31(1) : 3-4.). [This research has been recommended In F1000Prime, 17 Nov 2014; DOI: 10.3410/f.719709901.793501476. F1000Prime.com/719709901#eval793501476]

21.   Xu Y., Li H., Jin YH., Fan J.* and Sun F.* (2014), Dimerization interface of 3-hydroxyacyl-CoA dehydrogenase tunes the formation of its catalytic intermediate. PLOS one. 9(4): e95965. doi:10.1371/journal.pone.0095965.

22.   Ma J. and Sun F.* (2014), Expression, purification, crystallization and preliminary crystallographic study of the cytoplasmic domain of the mitochondrial dynamics protein MiD51. Acta Crystallographica Section F. F70: 596-599. doi:10.1107/S2053230X14006827.

23.   Sun F., Li J. and Chen C.* (2014), Puzzle out the regulation mechanism of PI4KIIα activity. Sci China Life Sci. doi: 10.1007/s11427-014-4656-8.

24.   Zhou Q., Li J., Yu H., Zhai Y., Gao Z., Liu Y., Pang X., Zhang L., Schulten K., Sun F.* and Chen C.* (2014), Molecular insights into the membrane-associated phosphatidylinositol 4-kinase IIα. Nature Communications, 5:3552. doi:10.1038/ncomms4552. [CAS News] [This research has been recommended In F1000Prime, 18 Jul 2014; DOI: 10.3410/f.718326221.793497426. F1000Prime.com/718326221#eval793497426]

25.   Qiu B., Zhang K., Wang S., Sun F.* (2014). C-terminal motif within Sec7 domain regulates guanine nucleotide exchange activity via tuning protein conformation.Biochem Biophys Res Commun, 446(1) : 380-386. doi: 10.1016/j.bbrc.2014.02.125.

26.   Han R., Zhang F., Wan X., Fernandez J.J., Sun F. and Liu Z. (2014). A marker-free automatic alignment method based scale-invariant features. Journal of Structural Biology, 186 : 167-180. doi: 10.1016/j.jsb.2014.02.011.

27.   Cheng, L., Huang, X., Li, X., Xiong, W., Sun, W., Yang, C., Zhang, K., Wang, Y., Liu, H., Huang, X., Ji, G., Sun F., Zheng, C.* and Zhu, P.* (2014). Cryo-EM structures of two bovine adenovirus type 3 intermediates. Virology,450-451 :  174-181.

28.   Zhang, Y., and Sun F.* (2013). Electron Microscopy Reconstruction of Helical Assemblies. Acta Biophys Sin, 29: 879-898.

29.   http://192.168.1.20/LBEMSB/Media/transparent.gifhttp://192.168.1.20/LBEMSB/Media/transparent.gifhttp://192.168.1.20/LBEMSB/Media/transparent.gifhttp://192.168.1.20/LBEMSB/Media/transparent.gifhttp://192.168.1.20/LBEMSB/Media/transparent.gifhttp://192.168.1.20/LBEMSB/Media/transparent.gifSun F.*, Zhou Q., Pang X., Xu Y. and Rao Z. (2013), Revealing various coupling of electron transfer and proton pumping in mitochondrial respiratory chain. Current Opinion in Structural Biology, 23: 526-538. doi: 10.1016/j.sbi.2013.06.013.

30.   http://192.168.1.20/LBEMSB/Media/transparent.gifhttp://192.168.1.20/LBEMSB/Media/transparent.gifhttp://192.168.1.20/LBEMSB/Media/transparent.gifhttp://192.168.1.20/LBEMSB/Media/transparent.gifZhang K., Wang L., Liu Y., Chan K.Y., Pang X., Schulten K., Dong Z. and Sun F.* (2013), Flexible interweaved termini determine the thermal stability of thermosomes. Protein Cell, 4(6): 432-444. doi: 10.1007/s13238-013-3026-9.

31.   Zhang Y., Wang W., Chen J., Zhang K., Gao F., Gao B., Zhang S., Dong M., Besenbacher F., Gong W., Zhang M., Sun F.* and Feng W.* (2013), Structural insights into the intrinsic self-assembly of Par-3 N-terminal domain. Structure, 21(6):997-1006. doi: 10.1016/j.str.2013.04.004.

32.   Lu J., Zhai Y. andSun F.* (2013), Research of Mitochondrial Calcium Transportation.ACTA BIOPHYSICA SINICA, 29(3): 167-180.

33.   Xu Y. and Sun F.* (2013), Purification, crystallization and preliminary crystallographic analysis of 3-hydroxyacyl-CoA dehydrogenase from Caenorhabditis elegans. Acta Crystallographica Section F, F69: 515-519. doi:10.1107/S1744309113007045.

34.   Wu L., Zhai Y., Lu J., Wang Q. and Sun F.* (2013), Expression, purification and preliminary characterization of glucagon receptor extracellular domain. Protein Expression and Purification, 89: 232-240. doi: 10.1016/j.pep.2013.04.004.

35.   Quinlan R., Zhang Y., Lansbury A., Williamson I., Pohl E.* and Sun F.* (2013), Changes in the quaternary structure and function of MjHSP16.5 attributable to deletion of the I-X-I motif and introduction of the substitution, R107G in the alpha-crystallin domain. Philosophical Transactions of The Royal Society B, 368. doi:10.1098/rstb.2012.0327.

36.   Wang X., Xu F., Liu J., Gao B., Liu Y., Zhai Y., Ma J., Zhang K., Baker T.S., Schulten K., Zheng D.*, Pang H.* and Sun F.* (2013), Atomic model of rabbit hemorrhagic disease virus by cryo-electron microscopy and crystallography. PLoS Pathogens, 9(1): e1003132. doi: 10.1371/journal.ppat.1003132. [NSFC News][China Science Daily News][NCSA of UIUC News]

37.   Zhang S., Andreasen M., Nielsen J.T., Liu L., Nielsen E.H., Song J., Ji G., Sun F., Skrydstrup T., Besenbacher F., Nielsen N.C., Otzen D.E and Dong M.* (2013), Coexistence of ribbon and helical fibrils originating from hIAPP20-29 revealed by quantitative nanomechanical atomic force microscopy. PNAS, doi:10.1073/pnas.1209955110.

38.   Wang L., Li Q., Wu L., Liu S., Zhang Y., Yang X., Zhu P., Zhang H., Zhang K., Lou J., Liu P., Tong L.*, Sun F.* and Fan Z.* (2013), Identification of SERPINB1 as a physiological inhibitor of human granzyme H. The Journal of Immunology, 190(3):1319-30. doi:10.4049/jimmunol.1202542.

39.   Yang S., Liu X., Li, X., Sun S.F., Sun F., Fan B., and Zhao S.* (2013), MicroRNA-124 reduces caveolar density by targeting caveolin-1 in porcine kidney epithelial PK15 cells. Molecular and cellular biochemistry 384, 213-219.

40.   Wang G., Zhang F., Fan L., Sun F. and Liu, Z. (2013) Contrast Transfer Function Correction Model Based on Sine and Gaussian Modulation and Spline Interpolation. Journal of Computer Research and Development 50, 808-814.

41.   Wang G., Zhang F., Fan L., Sun F. and Liu, Z. (2013) A fast calculation strategy of density function in ISAF reconstruction algorithm. Scientia Sinica Informationis 43, 584-598.

42.   Zhao Y., Zhao H., Miao L., Wang L., Sun F. and Zhang H. (2012), The p53-induced gene Ei24 is an essential component of the basal autophagy pathway. Journal of Biological Chemistry, 287(50):42053-63.

43.   Wang S. and Sun F.* (2012), Dynactin is an indispensable helper for dynein’s function in intracellular motility. ACTA BIOPHYSICA SINICA, 28(10):785-793.

44.   Bai M., Pang X., Lou J., Zhou Q., Zhang K., Ma J., Li J., Sun F.* Hsu V.* (2012), Mechanistic insights into regulated cargo binding by ACAP1.  Journal of Biological Chemistry, 287: 28675-28685.

45.   Hu J., Sun L., Shen F., Chen Y., Hua Y., Liu Y., Zhang M., Hu Y., Wang Q., Xu W., Sun F., Ji J., Murray J., Carr A., Kong D.* (2012), The Intra-S phase checkpoint targets Dna2 to prevent stalled replication forks from reversing. Cell, 149: 1221-1232.

46.   Yang C., Ji G., Liu H., Zhang K., Liu G., Sun F., Zhu P.*, Cheng L*. (2012), Cryo-EM structure of a transcribing cypovirus. PNAS, 109(16): 6118-6123.

47.   Wang G., Zhang F.,Sun F., Liu Z. (2012), An accelerated ISAF algorithm with the fast mapping strategy. Journal of Computers, 7(2): 528-533.

48.   Guo H., Yuan X., Liu J., Shan G., Chi X., Sun F. (2012), Interference microscopy volume illustration for biomedical data. Pacific Visualization Symposium (PacificVis), 2012 IEEE. 177-184. doi: 10.1109/PacificVis.2012.6183589.

49.   Wang L., Zhang K., Wu L., Liu S., Zhang H., Zhou Q., Tong L., Sun F.* and Fan Z.*. (2012), Structural Insights into the substrate specificity of human granzyme H - the functial roles of a novel RKR motif. The Journal of Immunology, 188(2): 765-73.

50.   Wang X., Wang L., Wang Xi., Sun F.* and Wang C.C.*.(2012), Structural insights into the peroxidase activity and inactivation of human peroxiredoxin 4. Biochem. J.,441: 113-118.

51.    Cheng L., Sun J., Zhang K., Mou Z., Huang X., Ji G., Sun F., Zhang J.*, Zhu P.* (2011), Atomic model of a cypovirus built from cryo-EM structure provides insight into the mechanism of mRNA capping. PNAS, 108(4):1373-1378.

52.    Wang L., Zhang K., Fan Z., Dong Z., Sun F.* (2011), Substrate binding properties of thermosome ATcpn-beta from Acidianus Tengchongensis. Progress in Biochemistry and Biophysics, 38(2):151-158.

53.    Zhai Y., Zhang K., Huo Y., Zhu Y., Zhou Q., Lu J., Black I., Pang X., Roszk A.W., Zhang X., Isaacs N.W., Sun F.* (2011), Autotransporter passenger domain secretion requires a hydrophobic cavity at the extracellular entrance of the beta-domain pore. Biochem. J. , 435:577-587. (This paper was featured as a key scientific article by Global Medial Discovery.))

54.    Chu Q., Zhang K., Wan X., Zhang C., Zhang Y., Zhang G., Shen B., Lu X., Zhao K.,Chu X., Zhang F.*, Sun F.* (2011), ATOM1.0 : A GPU Powered Package for Electron Tomography Reconstruction. ACTA BIOPHYSICA SINICA, 27(3):231-241. (The software ATOM1.0 could be found here.)

55.    Zheng X., Guo J., Xu L., Li H., Zhang D., Zhang K.,Sun F., Wen T., Liu S., Pang H.* (2011), Crystal Structure of a Novel Esterase Rv0045c from Mycobacterium tuberculosis. PLoS ONE, 6(5): e20506. doi:10.1371/journal.pone.0020506

56.    Wan X., Zhang F., Chu Q., Zhang K., Sun F., Yuan B., Liu Z.* (2011), Three-dimensional reconstruction using an adaptive simultaneous algebraic reconstruction technique in electron tomography. Journal of Structural Biology , 175(3):277-87. doi:10.1016/j.jsb.2011.06.002

57.    Zhou Q., Zhai Y., Lou J., Liu M., Pang X. and Sun F.* (2011), Thiabendazole inhibits ubiquinone reduction activity of mitochondrial respiratory complex II via a water molecule mediated binding feature. Protein & Cell, 2(7):531-542, doi:10.1007/s13238-011-1079-1 (cover story).

58.    Li M., Xu G.*, Sorzano, O.S.C., Sun F. and Bajaj L.C. (2011), Single-particle reconstruction using L2-gradient flow. Journal of Structural Biology,176:259-267. doi:10.1016/j.jsb.2011.08.005.

59.    Sun Fei* and Wang Xue. (2011), Application and aspect of cryo-electron microscopy for the structural study of membrane proteins. Chinese Bulletin of Life Sciences, 23(11):1130-1139.

60.    Chen Shaohong, Pang Xiaoyun and Sun Fei*. (2011), Mitochondrial movement and its related cytoskeletons and proteins. ACTA BIOPHYSICA SINICA, 27(12):1019-1029.

61.    Wang G., Zhang F., Fan L., Sun F., Liu Y. (2011), Analysis and solution of complexity of basis function in ISAF reconstruction algorithm. Journal of Computer-Aided Design & Computer Graphics. 23 (7) : 1148-1158.

62.    Li Z., Zhai Y., Fang J., Zhou Q., Geng Y., Sun F.* (2010), Purification, crystallization and preliminary crystallographic analysis of very long chain Acyl-CoA dehydrogenase from Caenorhadbitis elegans. Acta Crystallographica Section F, F66: 426-430.

63.    Wang L., Hu Z., Luo Y., Huo Y., Ma Q., He Y., Zhang Y., Sun F.*, Dong Z.* (2010), Distinct symmetry and limited peptide refolding activity of the thermosomes from the acidothermophilic archaea Acidianus tengchongensis S5T, Biochem Biophys Res Commun, 393: 228-234.

64.    Li Z., Zhang K., Zhai Y., Zhou Q., Geng Y., Sun F.* (2010), Cloning, Expression, Purification and Preliminary Crystallographic Analysis of Caenorhabditis elegnas Enoyl-CoA Hydratase. Acta Biophysica Sinica, 26(1):37-48.

66.    Hu Z., Tian X., Zhai Y., Xu W., Zheng D., Sun F.* (2010), Cryo-Electron Microscopy Reconstructions of Two Types of Wild Rabbit Hemorrhagic Disease Viruses Characterized the Structural Features of Lagovirus. Protein & Cell, 1(1):48-58. (Cover story).

67.    Zhou Q., Sun J., Zhai Y., Sun F.* (2010). Prokaryotic Expression of Active Mitochondrial Uncoupling Protein 1. Progress in Biochemistry and Biophysics, 37(1): 56~62.

68.    Li S., Zhao Q., Zhou Q., Unno H., Zhai Y., Sun F.*(2010). The Role and Structure of Carboxyl-terminal Domain of the Human Voltage-gated Proton Channel HV1. Journal of Biological Chemistry, 285 (16): 12047-12054.

69.    hang S., Zhang K., Chen X., Chu X., Sun F.*, Dong Z. (2010), Five mutations in N-terminus confer thermostability on mesophilic xylanase, BBRC, 395: 200-206.

70.    Lu B., Zhai Y., Wu C., Pang X., Xu Z. and Sun F.* (2010), Expression, purification and preliminary biochemical studies of the N-terminal domain of leucine-rich repeat kinase 2, Biochimica et Biophysica Acta, 1804: 1780-1784.

71.    Zhang K., Zhang Y., Hu Z., Gang J. and Sun F.* (2010), Development and Frontier of Electron Microscopy 3D Reconstruction. ACTA BIOPHYSICA SINICA, 26(7): 533-559. (cover story)

72.    71.Zhang Y, Liu D, Chen X, Li J, Li L, Bian Z, Sun F., Lu J, Yin Y, Cai X, Sun Q, Wang K, Ba Y, Wang Q, Wang D, Yang J, Liu P, Xu T, Yan Q, Zhang J, Zen K, Zhang CY. (2010). Secreted monocytic miR-150 enhances targeted endothelial cell migration. Mol. Cell, 39(1):133-44.

73.    Hu YW, Hu ZJ, Zhang K, Wang L, Zhai Y, Zhou QJ, Lander G, Zhu J, He YZ, Pang XY, Xu W, Bartlam M, Dong ZY, Sun F.*. (2010). Crystal structure of group II chaperonin in the open state. Structure, 18(10): 1270-1279. (cover story)

74.    Ma J, Sun F.*(2010). Translocation of Mitochondrial Proteins. ACTA BIOPHYSICA SINICA, 26(10): 880-893.

75.    Xu L., Guo J., Zheng X., Wen T., Sun F., Liu, S., and Pang, H.* (2010). Crystallization and preliminary X-ray analysis of a novel esterase Rv0045c from Mycobacterium tuberculosis. Acta Crystallogr Sect F Struct Biol Cryst Commun 66, 1579-1582.

76.    Zhang K,, Zhang C,, Sun F*. (2009) Disclosing the Structure and Function of Translation Machine - Introduction of the Noble Prize in Chemistry 2009. Acta Biophysica Sinica, 25(5):325-334.

77.    Wu L., Wang L., Hua G., Liu K., Yang X., Zhai Y., Bartlam M., Sun F*. and Fan Z.* (2009) Structural Basis for Proteolytic Specificity of the Human Apoptosis-Inducing Granzyme M, The Journal of Immunology, 183, 421-9.

78.    Sun S.Zhang K., Xu W.Wang G.Chen J. and Sun F*. (2009) 3D Structural Investigation of Caveolae From Porcine Aorta Endothelial Cell by Electron Tomography, Progress in Biochemistry and Biophysis, 36(6), 729-35.

79.    Sun, F.*#, Zhou, Q. J., Sun, J., Zhai, Y. J. & Rao, Z. H. (2008) Structure of mitochondrial respiratory membrane protein complexes, Chinese Bulletin of Life Sciences.20.

80.    Wang, L., Wang, L., Vavassori, S., Li, S., Ke, H., Anelli, T., Degano, M., Ronzoni, R., Sitia, R., Sun, F.* & Wang, C. C.* (2008) Crystal structure of human ERp44 shows a dynamic functional modulation by its carboxy-terminal tail, EMBO Rep. 9, 642-7.

81.    Chen, J., Li, W., Wang, M., Zhu, G., Liu, D., Sun, F., Hao, N., Li, X., Rao, Z. & Zhang, X. C. (2008) Crystal structure and mutagenic analysis of GDOsp, a gentisate 1,2-dioxygenase from Silicibacter pomeroyi, Protein Sci. 17, 1362-73.

82.    Huo X, Su D, Wang A, Zhai Y, Xu J, Li X, Bartlam M, Sun F*, Rao Z. 2007. Preliminary molecular characterization and crystallization of mitochondrial respiratory complex II from porcine heart , FEBS JOURNAL, 274(6): 1524-1529

83.    Zheng, W., Sun, F., Bartlam, M., Li, X., Li, R. & Rao, Z. (2007) The crystal structure of human isopentenyl diphosphate isomerase at 1.7 a resolution reveals its catalytic mechanism in isoprenoid biosynthesis, J Mol Biol.366, 1447-58.

84.    Su D, Lou Z, Sun F, Zhai Y, Yang H, Zhang R, Joachimiak A, Zhang XC, Bartlam M, Rao Z. 2006. Dodecamer Structure of Severe Acute Respiratory Syndrome Coronavirus Nonstructural Protein nsp10. J Virol. 80(16):7902-8.

85.    Xu X, Zhai Y, Sun F, Lou Z, Su D, Xu Y, Zhang R, Joachimiak A, Zhang XC, Bartlam M, Rao Z. 2006. New Antiviral Target Revealed by the Hexameric Structure of Mouse Hepatitis Virus Nonstructural Protein nsp15. J Virol.80(16):7909-17.

86.    Li X, Liu X, Sun F, Gao J, Zhou H, Gao GF, Bartlam M, Rao Z. 2006. Crystal structure of the N-terminal SH3 domain of mouse betaPIX, p21-activated kinase-interacting exchange factor. Biochem Biophys Res Commun. 339(1):407-14.

87.    孙飞#,徐建兴,饶子和.2005.膜蛋白三维结构研究的新突破-线粒体呼吸链膜蛋白复合物II结构解析.中国科学院院刊. 20055):381384

88.    Zhai Y#, Sun F#, Li X, Pang H, Xu X, Bartlam M & Rao Z. 2005. Insights into SARS-CoV transcription and replication from the structure of the nsp7-nsp8 hexadecamer. Nat. Struct. Mol. Biol. 12(11): 980986

89.    Sun F#, Huo X, Zhai Y, Wang A, Xu J, Su D, Bartlam M& Rao Z. 2005. Crystal structure of mitochondrial respiratory membrane protein complex II. Cell, 121: 1043-1057.

90.    Shi N, Ye S, Bartlam M, Yang M, Wu J, Liu Y, Sun F, Han X, Peng X, Qiang B, Yuan J & Rao Z. 2004. Structural Basis for the Specific Recognition of RET by the Dok1 Phosphotyrosine Binding Domain. J Biol Chem.,279(6): 4962-9

91.    Shi N, Ni M, Yang M, Wu J, Peng Y, Gao F, Sun F, Peng X, Qiang B, Rao Z & Yuan J. 2004. Expression, crystallization and preliminary X-ray studies of the recombinant PTB domain of mouse dok-1 protein. Acta Cryst., D60(2): 334-336

92.    Sun F#, Li P, Ding Y, Wang L, M Bartlam, Shu C, Shen B, Jiang H, Li S & Rao Z. 2003. Desian and Structure Based Study of New Potential FKBP12 Inhibitors. Biophysical Journal, 85 (5): 3194-3201.  

93.    Ding Y, Li S, Li XF, Sun F, Liu JY, Zhao NM & Rao Z. 2003. Site-directed Mutagenesis and Preliminary X-ray Crystallographic Studies of the Tabtoxin Resistance Protein. Protein and Peptide Letters, 10(3): 255-263

94.    Lin W, Sun F& Rao Z 2003. A Statistical Analysis of Protein-Protein Interaction with Knowledge-Based Potential at Residue Level. Tsinghua Science and Technology, 8(4): 402-406

95.    Li T, Sun F, Ji X, Feng Y & Rao Z. 2003. Structure Based Hyperthermostability of Archaeal Histone HPhA from Pyrococcus horikoshii. J Mol Biol., 325: 1031-1037

96.    He H, Ding Y, Bartlam M, Sun F, Le Y, Qin X, Tang H, Zhang R, Joachimiak A, Liu J, Zhao N&Rao Z. 2003. Crystal Structure of Tabtoxin Resistance Protein Complexed with Acetyl Coenzyme A Reveals the Mechanism for β­Lactam Acetylation. J Mol Biol., 325(5): 1019-1030

科研活动

   
科研项目(按时间倒序排列)

1. 国家973计划“蛋白质机器及分子机制”(2006CB806500),2006/09-2010/08,已结题,子课题负责人。
2. 国家863计划“富亮氨酸重复激酶2(LRRK2)突变的致病机制及其作为帕金森氏病药物靶标的可行性研究”(2006AA02Z173),2006/12-2008/12,已结题,参加。
3. 国家重大研究计划“蛋白质研究计划”“膜蛋白和蛋白质复合体的功能与结构研究”(2006CB911000),2006/12-2010/12,已结题,参加。
4. 国家重点实验室优秀基金(基金委重点项目)“通过基因敲除和三维结构来揭示重要蛋白复活物的体内功能”(30623005 ),2007/01-2010/12,已结题,参加。
5. 自然科学基金委面上项目“应用低温电镜和晶体学方法对古菌II类分子伴侣的结构解析”(30770496),2008/01-2010/12,已结题,主持。
6. 中国科学院知识创新工程重要方向项目“冷冻电子断层三维成像技术建立及其应用”(KSCX2-YW-R-126),2007/10-2010/09,已结题,主持。
7. 中国科学院知识创新工程重大项目“面向蛋白质科学的高性能计算研究”(KGGX1-YW-13),2007/12-2010/12,已结题,子课题负责人。
8. 自然科学基金委创新群体“膜蛋白与蛋白质复合体的结构生物学研究”(30721003),2008/01-2010/12,已结题,参加。
9. 自然科学基金委创新群体“膜蛋白与蛋白质复合体的结构生物学研究”(31021062),2011/01-2013/12,已结题,参加。
10. 国家973计划“重要生命活动中关键膜蛋白及蛋白质复合物结构与功能研究”(2011CB910300),2011/01-2015/12,在研(2015年结题),参加(学术骨干)。
11. 自然科学基金委面上项目“二型分子伴侣素的动态结构与协同性”(31170700),2012/01-2015/12,在研(2015年结题),主持。
12. 中组部“万人计划”青年拔尖人才,2013/01-2015/12,在研,主持。
13. 国家973计划“青年科学家项目”“复杂生物大分子复合体的低温电镜高分辨三维重构及功能研究”(2014CB910700),2014/01-2018/12,在研,项目首席科学家。
14. 中国科学院战略性先导科技专项(B类)“超大分子复合体前沿技术”(XDB08030200),2014/01-2018/12,在研,项目首席科学家。

合作情况

   
项目协作单位
   

指导学生

已指导学生

欧阳珠清  01  19183  

胡仲军  02  19182  

霍艳武  01  19183  

周强军  01  19183  

徐颖之  01  19184  

马军  01  19184  

邱彪  01  19184  

张凯  01  19184  

庞效云  01  19184  

高丙全  01  19183  

陆久维  01  19183  

王圣柳  01  19183  

陈文博  02  19183  

邓雨辰  01  19183  

王雪  01  19183  

现指导学生

牛彤欣  02  19184  

朱春梅  02  19184  

师扬  01  19184  

玉蕾叶  01  19183  

张丹阳  01  19183  

台林华  02  19183  

杨静媛   02  19179  

张双博   02  63228  

孙芳  01  19184