基本信息

李德峰  男  博导  中国科学院微生物研究所
电子邮件: lidefeng@im.ac.cn
通信地址: 北京市朝阳区北辰西路1号院3号中国科学院微生物研究所
邮政编码: 100101

研究领域

基于结构生物学的微生物环境适应机制研究


招生信息

   
招生专业
071010-生物化学与分子生物学
071005-微生物学
招生方向
微生物适应环境的分子机理研究
结构生物学

教育背景

2001-09--2006-06   中国科学院生物物理研究所   理学博士
1997-09--2001-07   武汉大学   理学学士
学历
研究生

学位
博士

工作经历

   
工作简历
2018-02~现在, 中国科学院微生物研究所, 项目研究员
2006-07~2018-01,中国科学院生物物理研究所, 助理研究员/副研究员/项目研究员

专利与奖励

   
奖励信息
(1) 中国科学院青年创新促进会会员, 院级, 2014

出版信息

   
发表论文
[1] MengRu Wu, LiLi Miao, Ying Liu, XinXin Qian, TingTing Hou, GuoMin Ai, Lu Yu, Lan Ma, XiYan Gao, YaLing Qin, HaiZhen Zhu, Lei Du, ShengYing Li, ChangLin Tian, DeFeng Li, ZhiPei Liu, ShuangJiang Liu. Identification and characterization of a novel hydroxylamine oxidase, DnfA, that catalyzes the oxidation of hydroxylamine to N 2. THE JOURNAL OF BIOLOGICAL CHEMISTRY[J]. 2022, 298(9): https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9478400/.
[2] JiaWei Qian, XiaoYan Wang, Kai Deng, DeFeng Li, Lu Guo. Crystal structure of the chromosome partition protein MukE homodimer. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS. 2022, 589: 229-233, http://dx.doi.org/10.1016/j.bbrc.2021.12.032.
[3] Liu, Feixia, Ren, Jie, Guo, Lu, Liu, Yang, Li, DeFeng, Yu, Bo. Desymmetric hydrolysis of prochiral imide for S-pregabalin synthesis by rationally designed d-hydantoinase. GREEN CHEMISTRY[J]. 2022, 24(12): 4748-4753, http://dx.doi.org/10.1039/d2gc00728b.
[4] Ma, HongLin Ma, Ding, Mai, Guo, Lu, Li, DeFeng. Structural insights into the substrate specificity of 5-chloro-2-hydroxymuconate tautomerase CnbG. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS[J]. 2022, 620: 42-48, http://dx.doi.org/10.1016/j.bbrc.2022.06.058.
[5] Xu, Anming, Wang, Di, Wang, Yunhao, Zhang, Li, Xie, Zhensheng, Cui, Yifan, Bhamse, Pramod, Yu, Haiying, Zhang, XueXian, Li, Defeng, 马旅雁. Mutations in surface-sensing receptor WspA lock the Wsp signal transduction system into a constitutively active state. ENVIRONMENTAL MICROBIOLOGY[J]. 2022, 24(3): 1150-1165, http://dx.doi.org/10.1111/1462-2920.15763.
[6] Wang, Yunhao, Huang, Zhou, Zhou, Nan, Liu, Chang, Jiang, Chengying, Li, Defeng, Liu, Shuangjiang. The Response Regulator FlmD Regulates Biofilm Formation in Comamonas testosteroni through the Transcriptional Activator SoxR. MICROORGANISMS[J]. 2022, 10(2): http://dx.doi.org/10.3390/microorganisms10020356.
[7] Xu, SiQiong, Qian, XinXin, Jiang, YinHu, Qin, YaLing, Zhang, FuYin, Zhang, KaiYun, Hong, Qing, He, Jian, Miao, LiLi, Liu, ZhiPei, Li, DeFeng, Liu, ShuangJiang, Qiu, JiGuo. Genetic Foundations of Direct Ammonia Oxidation (Dirammox) to N-2 and MocR-Like Transcriptional Regulator DnfR in Alcaligenes faecalis Strain JQ135. APPLIED AND ENVIRONMENTAL MICROBIOLOGY[J]. 2022, 88(6): http://dx.doi.org/10.1128/aem.02261-21.
[8] Chen, Feilong, Liu, Beibei, Zeng, Jianwei, Guo, Lu, Ge, Xuan, Feng, Wei, Li, DeFeng, Zhou, Hao, Long, Jiafu. Crystal Structure of the Core Module of the Yeast Paf1 Complex. JOURNAL OF MOLECULAR BIOLOGY[J]. 2022, 434(2): 167369-, http://dx.doi.org/10.1016/j.jmb.2021.167369.
[9] Yang, ZhenNi, Liu, ZeShen, Wang, KeHuan, Liang, ZongLin, Abdugheni, Rashidin, Huang, Ye, Wang, RunHua, Ma, HongLin, Wang, XiaoKang, Yang, MeiLing, Zhang, BingGe, Li, DeFeng, Jiang, ChengYing, Corvini, Philippe FX, Liu, ShuangJiang. Soil microbiomes divergently respond to heavy metals and polycyclic aromatic hydrocarbons in contaminated industrial sites. ENVIRONMENTAL SCIENCE AND ECOTECHNOLOGY[J]. 2022, 10(2): 82-90, https://doaj.org/article/cff2f76e4ac64e09bd6dd77ad1362bf7.
[10] Wang, YunHao, Chen, HongHe, Huang, Zhou, Li, XiaoJing, Zhou, Nan, Liu, Chang, Jiang, ChengYing, Li, DeFeng, Liu, ShuangJiang. PapA, a peptidoglycan-associated protein, interacts with OmpC and maintains cell envelope integrity. ENVIRONMENTAL MICROBIOLOGY[J]. 2021, 23(2): 600-612, http://dx.doi.org/10.1111/1462-2920.15038.
[11] Hou, YanJie, Guo, Yuan, Li, DeFeng, Zhou, NingYi. Structural and Biochemical Analysis Reveals a Distinct Catalytic Site of Salicylate 5-Monooxygenase NagGH from Rieske Dioxygenases. APPLIED AND ENVIRONMENTAL MICROBIOLOGY[J]. 2021, 87(6): http://dx.doi.org/10.1128/AEM.01629-20.
[12] Guo, Yuan, Li, DeFeng, Zheng, Jianting, Xu, Ying, Zhou, NingYi. Single-Component and Two-Component para-Nitrophenol Monooxygenases: Structural Basis for Their Catalytic Difference. APPLIED AND ENVIRONMENTAL MICROBIOLOGY[J]. 2021, 87(22): [13] Wu, MengRu, Hou, TingTing, Liu, Ying, Miao, LiLi, Ai, GuoMin, Ma, Lan, Zhu, HaiZhen, Zhu, YaXin, Gao, XiYan, Herbold, Craig W, Wagner, Michael, Li, DeFeng, Liu, ZhiPei, Liu, ShuangJiang. Novel Alcaligenes ammonioxydans sp. nov. from wastewater treatment sludge oxidizes ammonia to N-2 with a previously unknown pathway. ENVIRONMENTAL MICROBIOLOGY[J]. 2021, 23(11): 6965-6980, http://apps.webofknowledge.com/CitedFullRecord.do?product=UA&colName=WOS&SID=5CCFccWmJJRAuMzNPjj&search_mode=CitedFullRecord&isickref=WOS:000700823100001.
[14] Guo, Yuan, Li, DeFeng, Ji, Huining, Zheng, Jianting, Zhou, NingYi. Hexachlorobenzene Monooxygenase Substrate Selectivity and Catalysis: Structural and Biochemical Insights. APPLIED AND ENVIRONMENTAL MICROBIOLOGY[J]. 2021, 87(1): https://www.webofscience.com/wos/woscc/full-record/WOS:000605458700015.
[15] Chen, Feilong, Liu, Beibei, Guo, Lu, Ge, Xuan, Feng, Wei, Li, De-Feng, Zhou, Hao, Long, Jiafu. Biochemical insights into Paf1 complex-induced stimulation of Rad6/Bre1-mediated H2B monoubiquitination. Proc. Natl. Acad. Sci. USA[J]. 2021, 118(33): e2025291118-, [16] Wang, Xiaoyan, Gu, Xien, Li, Jie, Yue, Lei, Li, Defeng, Dong, Xiuzhu. Characterization of the Methanomicrobial Archaeal RNase Zs for Processing the CCA-Containing tRNA Precursors. FRONTIERS IN MICROBIOLOGY[J]. 2020, 11: https://doaj.org/article/f5d7a31bf8394c90abb1dafabbe0465e.
[17] Wang XiaoYan, Qin YaLing, Han Qun, Gu XiEn, Yan Zi, Fu Kui, Li DeFeng, Deng Kai. A New State I-plus Observed for the L, D- transpeptidase LdtMt2-ertapenem Adduct. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS[J]. 2020, 47(9): 989-997, https://www.webofscience.com/wos/woscc/full-record/WOS:000573116600006.
[18] Hou, YanJie, Yang, WenSi, Hong, Yuan, Zhang, Ying, Wang, DaCheng, Li, DeFeng. Structural insights into the mechanism of c-di-GMP?bound YcgR regulating flagellar motility in Escherichia coli. JOURNAL OF BIOLOGICAL CHEMISTRY[J]. 2020, 295(3): 808-821, https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6970932/.
[19] Li, Jie, Hou, Yanjie, Gu, Xien, Yue, Lei, Guo, Lu, Li, Defeng, Dong, Xiuzhu. A newly identified duplex RNA unwinding activity of archaeal RNase J depends on processive exoribonucleolysis coupled steric occlusion by its structural archaeal loops. RNA BIOLOGY[J]. 2020, 17(10): 1480-1491, http://apps.webofknowledge.com/CitedFullRecord.do?product=UA&colName=WOS&SID=5CCFccWmJJRAuMzNPjj&search_mode=CitedFullRecord&isickref=WOS:000545799500001.
[20] Wu, Huijun, Qiao, Shuai, Li, Defeng, Guo, Lu, Zhu, Meijun, Ma, Luyan Z. Crystal structure of the glycoside hydrolase PssZ from Listeria monocytogenes. ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS[J]. 2019, 75(Pt 7): 501-506, https://www.webofscience.com/wos/woscc/full-record/WOS:000474470400005.
[21] Zhao, Fen, Hou, YanJie, Zhang, Ying, Wang, DaCheng, Li, DeFeng. The 1-beta-methyl group confers a lower affinity of L,D-transpeptidase Ldt(Mt2) for ertapenem than for imipenem. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS[J]. 2019, 510(2): 254-260, http://dx.doi.org/10.1016/j.bbrc.2019.01.082.
[22] Hong, Yuan, Huang, Zhou, Guo, Lu, Ni, Bin, Jiang, ChengYing, Li, XiaoJing, Hou, YanJie, Yang, WenSi, Wang, DaCheng, Zhulin, Igor B, Liu, ShuangJiang, Li, DeFeng. The ligand-binding domain of a chemoreceptor from Comamonas testosteroni has a previously unknown homotrimeric structure. MOLECULAR MICROBIOLOGY[J]. 2019, 112(3): 906-917, [23] 向华. Huang Z, Wang YH, Zhu HZ, Andrianova EP, Jiang CY, Li D, Ma L, Feng J, Liu ZP, Xiang H, Zhulin IB, Liu SJ.Cross Talk between Chemosensory Pathways That Modulate Chemotaxis and Biofilm Formation.. MBio. ;10(1). pii: e02876-18.. 2019, [24] Zhou Huang, YunHao Wang, HaiZhen Zhu, Ekaterina P Andrianova, ChengYing Jiang, Defeng Li, Luyan Ma, Jie Feng, ZhiPei Liu, Hua Xiang, Igor B Zhulin, ShuangJiang Liu. Cross Talk between Chemosensory Pathways That Modulate Chemotaxis and Biofilm Formation. MBIO[J]. 2019, 10(1): https://doaj.org/article/8de476d42ca74d7ca235f0d3d07dcf7c.
[25] Zheng, Xinxin, Cui, Yinglu, Li, Tao, Li, Ruifeng, Guo, Lu, Li, Defeng, Wu, Bian. Biochemical and structural characterization of a highly active branched-chain amino acid aminotransferase from Pseudomonas sp. for efficient biosynthesis of chiral amino acids. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY[J]. 2019, 103(19): 8051-8062, http://dx.doi.org/10.1007/s00253-019-10105-9.
[26] Yang Yan, Sun YuLing, Liu Pei, Qian LinNan, Guan DongLang, Jian TunYu, Zhu Chan, Yu Guang, Wang ChangMing, Zhu Jing, Li DeFeng, Tang ZongXiang. Study on Itching Substance and Mechanism in Chinese Yam (Dioscoreae Rhizoma). PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS[J]. 2019, 46(10): 1012-1019, [27] Feng, Na, Qi, Chao, Hou, YanJie, Zhang, Ying, Wang, DaCheng, Li, DeFeng. The C2 '- and C3 '-endo equilibrium for AMP molecules bound in the cystathionine-beta-synthase domain. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS[J]. 2018, 497(2): 646-651, http://dx.doi.org/10.1016/j.bbrc.2018.02.124.
[28] Yang, WenSi, Hong, Yuan, Zhang, Ying, Wang, DaCheng, Li, DeFeng, Hou, YanJie. A potential substrate binding pocket of BdcA plays a critical role in NADPH recognition and biofilm dispersal. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS[J]. 2018, 497(3): 863-868, http://dx.doi.org/10.1016/j.bbrc.2018.02.143.
[29] Gao, Rongsui, Li, Defeng, Lin, Yuan, Lin, Jingxia, Xia, Xiaoyun, Wang, Hui, Bi, Lijun, Zhu, Jun, Hassan, Bachar, Wang, Shihua, Feng, Youjun. Structural and Functional Characterization of the FadR Regulatory Protein from Vibrio alginolyticus. FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY[J]. 2017, 7: https://doaj.org/article/d1c4877482fd4c6ba9859f33c0fba3bc.
[30] YunRong Gao, DeFeng Li, Joy Fleming, YaFeng Zhou, Ying Liu, JiaoYu Deng, Lin Zhou, Jie Zhou, GuoFeng Zhu, XianEn Zhang, DaCheng Wang, LiJun Bi. Structural analysis of the regulatory mechanism of MarR protein Rv2887 in M-tuberculosis. SCIENTIFIC REPORTS[J]. 2017, 7(1): http://www.corc.org.cn/handle/1471x/2373365.
[31] Wu, Yujie, Hu, Kuan, Li, Defeng, Bai, Lin, Yang, Shaoqing, Jastrab, Jordan B, Xiao, Shuhao, Hu, Yonglin, Zhang, Susan, Darwin, K Heran, Wang, Tao, Li, Huilin. Mycobacterium tuberculosis proteasomal ATPase Mpa has a -grasp domain that hinders docking with the proteasome core protease. MOLECULAR MICROBIOLOGY[J]. 2017, 105(2): 227-241, https://www.webofscience.com/wos/woscc/full-record/WOS:000404871400004.
[32] Zheng, Xin, Feng, Na, Li, Defeng, Dong, Xiuzhu, Li, Jie. New molecular insights into an archaeal RNase J reveal a conserved processive exoribonucleolysis mechanism of the RNase J family. MOLECULAR MICROBIOLOGY[J]. 2017, 106(3): 351-366, http://www.corc.org.cn/handle/1471x/2176886.
[33] Jian Sun, Yongchang Xu, Rongsui Gao, Jingxia Lin, Wenhui Wei, Swaminath Srinivas, Defeng Li, RunShi Yang, XingPing Li, XiaoPing Liao, YaHong Liu, Youjun Feng. Deciphering MCR-2 Colistin Resistance. MBIO[J]. 2017, 8(3): https://doaj.org/article/657b871a90764a21a6c5aad19ecf31d0.
[34] Gao, Rongsui, Hu, Yongfei, Li, Zhencui, Sun, Jian, Wang, Qingjing, Lin, Jingxia, Ye, Huiyan, Liu, Fei, Srinivas, Swaminath, Li, Defeng, Zhu, Baoli, Liu, YaHong, Tian, GuoBao, Feng, Youjun. Dissemination and Mechanism for the MCR-1 Colistin Resistance. PLOS PATHOGENS[J]. 2016, 12(11): http://dx.doi.org/10.1371/journal.ppat.1005957.
[35] Zhang, Huimin, Srinivas, Swaminath, Li, Defeng, Feng, Youjun. Origin, dissemination and entry of the pandemic Zika viruses. SCIENCE BULLETIN[J]. 2016, 61(10): 749-751, http://dx.doi.org/10.1007/s11434-016-1068-0.
[36] LiLi Miao, YanJie Hou, HongXia Fan, Jie Qu, Chao Qi, Ying Liu, DeFeng Li, ZhiPei Liu, J L Schottel. Molecular Structural Basis for the Cold Adaptedness of the Psychrophilic β-Glucosidase BglU in Micrococcus antarcticus. APPLIED AND ENVIRONMENTAL MICROBIOLOGY. 2016, 82(7): 2021-2030, [37] Yang, Kai, Li, DeFeng, Wang, Xie, Liang, Jinzhao, Sitia, Roberto, Wang, Chihchen, Wang, Xi. Crystal Structure of the ERp44-Peroxiredoxin 4 Complex Reveals the Molecular Mechanisms of Thiol-Mediated Protein Retention. STRUCTURE[J]. 2016, 24(10): 1755-1765, http://dx.doi.org/10.1016/j.str.2016.08.002.
[38] Miao, LiLi, Hou, YanJie, Fan, HongXia, Qu, Jie, Qi, Chao, Liu, Ying, Li, DeFeng, Liu, ZhiPei. Molecular Structural Basis for the Cold Adaptedness of the Psychrophilic beta-Glucosidase BglU in Micrococcus antarcticus. APPLIED AND ENVIRONMENTAL MICROBIOLOGY[J]. 2016, 82(7): 2021-2030, https://www.webofscience.com/wos/woscc/full-record/WOS:000373342400005.
[39] Liu, Yingying, Gao, Yunrong, Li, Defeng, Fleming, Joy, Li, Honglin, Bi, Lijun. Crystal structure of Rv3899c(184-410), a hypothetical protein from Mycobacterium tuberculosis. ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS[J]. 2016, 72(Pt 8): 642-645, https://www.webofscience.com/wos/woscc/full-record/WOS:000380772600010.
[40] Xie, XiaoQian, Zhang, XiaoLi, Qi, Chao, Li, DeFeng, Fleming, Joy, Wang, DaCheng, Bi, LiJun. Crystallographic observation of the movement of the membrane-distal domain of the T7SS core component EccB1 from Mycobacterium tuberculosis. ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS[J]. 2016, 72(Pt 2): 139-144, https://www.webofscience.com/wos/woscc/full-record/WOS:000369384100008.
[41] Gao, YunRong, Feng, Na, Chen, Tao, Li, DeFeng, Bi, LiJun. Structure of the MarR family protein Rv0880 from Mycobacterium tuberculosis. ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS[J]. 2015, 71(Pt 6): 741-745, http://www.corc.org.cn/handle/1471x/2376736.
[42] 王大成. Structure and function of Mycobacterium smegmatis 7-keto-8-aminopelargonic acid (KAPA) synthase. INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY[J]. 2015, 58: 71-80, http://dx.doi.org/10.1016/j.biocel.2014.11.006.
[43] Ren, XiaoMing, Li, DeFeng, Jiang, Shuai, Lan, XianQing, Hu, Yonglin, Sun, Hui, Wang, DaCheng. Structural Basis of Specific Recognition of Non-Reducing Terminal N-Acetylglucosamine by an Agrocybe aegerita Lectin. PLOS ONE[J]. 2015, 10(6): https://www.webofscience.com/wos/woscc/full-record/WOS:000358147500031.
[44] Qi, Chao, Li, DeFeng, Feng, Lei, Hou, Yanjie, Sun, Hui, Wang, DaCheng, Liu, Wei. Biochemical and structural characterization of a novel ubiquitin-conjugating enzyme E2 from Agrocybe aegeria reveals Ube2w family-specific properties. SCIENTIFIC REPORTS[J]. 2015, 5: https://www.webofscience.com/wos/woscc/full-record/WOS:000363961700001.
[45] Zhang, XiaoLi, Li, DeFeng, Fleming, Joy, Wang, LiWei, Zhou, Ying, Wang, DaCheng, Zhang, XianEn, Bi, LiJun. Core component EccB1 of the Mycobacterium tuberculosis type VII secretion system is a periplasmic ATPase. FASEB JOURNAL[J]. 2015, 29(12): 4804-4814, https://www.webofscience.com/wos/woscc/full-record/WOS:000365523600008.
[46] Shanghua Fan, De-Feng Li, Da-Cheng Wang, Joy Fleming, Hongtai Zhang, Ying Zhou, Lin Zhou, Jie Zhou, Tao Chen, Guanjun Chen, Xian-En Zhang, Lijun Bi. Structure and function of Mycobacterium smegmatis 7-keto-8-aminopelargonic acid (KAPA) synthase. INTERNATIONAL JOURNAL OF BIOCHEMISTRY AND CELL BIOLOGY. 2015, 58: 71-80, http://dx.doi.org/10.1016/j.biocel.2014.11.006.
[47] 王大成. Purification, crystallization and preliminary X-ray crystallographic studies of Rv3899c from Mycobacterium tuberculosis. ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS[J]. 2015, 71(Pt 1): 107-109, https://www.webofscience.com/wos/woscc/full-record/WOS:000349302700021.
[48] Xie, Ting, Hou, Yanjie, Li, Defeng, Yue, Yang, Qian, Shijun, Chao, Yapeng. Structural basis of a mutant Y195I alpha-cyclodextrin glycosyltransferase with switched product specificity from alpha-cyclodextrin to beta-/gamma-cyclodextrin. JOURNAL OF BIOTECHNOLOGY[J]. 2014, 182: 92-96, https://www.webofscience.com/wos/woscc/full-record/WOS:000338812100012.
[49] 王大成. Purification and X-ray crystallographic analysis of 7-keto-8-aminopelargonic acid (KAPA) synthase from Mycobacterium smegmatis. ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS[J]. 2014, 70(Pt 10): 1372-1375, https://www.webofscience.com/wos/woscc/full-record/WOS:000343060200013.
[50] Ren, Xiaoming, Jiang, Shuai, Li, Defeng, Sun, Hui, Wang, Dacheng. Crystallization and preliminary crystallographic studies of AAL-2, a novel lectin from Agrocybe aegerita that binds nonreducing terminal N-acetylglucosamine. ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS[J]. 2013, 69: 650-652, https://www.webofscience.com/wos/woscc/full-record/WOS:000319736000013.
[51] Li, PanPan, Li, DeFeng, Liu, Di, Liu, YiMing, Liu, Chang, Liu, ShuangJiang. Interaction between DAHP synthase and chorismate mutase endows new regulation on DAHP synthase activity in Corynebacterium glutamicum. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY[J]. 2013, 97(24): 10373-10380, https://www.webofscience.com/wos/woscc/full-record/WOS:000327495000012.
[52] Ren BaiGuang, Li DeFeng, Zheng CaiXia, Gai Ying, Jiang XiangNing, Hu YongLin. His-234 is an Essential Residue for Populus tomentosa 4-coumarate : CoA Ligase Enzymatic Mechanisms. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS[J]. 2013, 40(11): 1165-1172, https://www.webofscience.com/wos/woscc/full-record/WOS:000327576500011.
[53] Li, WenJuan, Li, DeFeng, Hu, YongLin, Zhang, XianEn, Bi, LiJun, Wang, DaCheng. Crystal structure of L,D-transpeptidase Ldt(Mt2) in complex with meropenem reveals the mechanism of carbapenem against Mycobacterium tuberculosis. CELL RESEARCH[J]. 2013, 23(5): 728-731, http://www.irgrid.ac.cn/handle/1471x/756672.
[54] Zhang, Xiaoli, Zhang, Jinyong, Zhang, Rui, Guo, Ying, Wu, Chao, Mao, Xuhu, Guo, Gang, Zhang, Ying, Li, Defeng, Zou, Quanming. Structural, enzymatic and biochemical studies on Helicobacter pylori arginase. INTERNATIONALJOURNALOFBIOCHEMISTRYCELLBIOLOGY[J]. 2013, 45(5): 995-1002, http://dx.doi.org/10.1016/j.biocel.2013.02.008.
[55] Liu, Qingbo, Li, Defeng, Hu, Yonglin, Wang, DaCheng. Expression, crystallization and preliminary crystallographic study of GluB from Corynebacterium glutamicum. ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS[J]. 2013, 69: 657-659, https://www.webofscience.com/wos/woscc/full-record/WOS:000319736000015.
[56] 王大成. Structures of aminophenol dioxygenase in complex with intermediate, product and inhibitor. ACTA CRYSTALLOGRAPHICA SECTION D-BIOLOGICAL CRYSTALLOGRAPHY[J]. 2013, 69: 32-43, http://www.irgrid.ac.cn/handle/1471x/756639.
[57] Li, DeFeng, Feng, Lei, Hou, YanJie, Liu, Wei. The expression, purification and crystallization of a ubiquitin-conjugating enzyme E2 from Agrocybe aegerita underscore the impact of His-tag location on recombinant protein properties. ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS[J]. 2013, 69: 153-157, https://www.webofscience.com/wos/woscc/full-record/WOS:000314467200016.
[58] Hou, Yanjie, Li, DeFeng, Wang, DaCheng. Crystallization and preliminary X-ray analysis of the flagellar motor 'brake' molecule YcgR with c-di-GMP from Escherichia coli. ACTACRYSTALLOGRAPHICASECTIONFSTRUCTURALBIOLOGYCOMMUNICATIONS[J]. 2013, 69: 663-665, https://www.webofscience.com/wos/woscc/full-record/WOS:000319736000017.
[59] Niu, Siqiang, Luo, Miao, Tang, Jian, Zhou, Hua, Zhang, Yangli, Min, Xun, Cai, Xuefei, Zhang, Wenlu, Xu, Wenchu, Li, Defeng, Ding, Jingjin, Hu, Yonglin, Wang, Dacheng, Huang, Ailong, Yin, Yibin, Wang, Deqiang. Structural Basis of the Novel S. pneumoniae Virulence Factor, GHIP, a Glycosyl Hydrolase 25 Participating in Host-Cell Invasion. PLOS ONE[J]. 2013, 8(7): http://dx.doi.org/10.1371/journal.pone.0068647.
[60] Li, DeFeng, Zhang, JiaYue, Hou, Yanjie, Liu, Lei, Liu, ShuangJiang, Liu, Wei. Crystallization and preliminary crystallographic analysis of 2-aminophenol 1,6-dioxygenase complexed with substrate and with an inhibitor. ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS[J]. 2012, 68: 1337-1340, http://apps.webofknowledge.com/CitedFullRecord.do?product=UA&colName=WOS&SID=5CCFccWmJJRAuMzNPjj&search_mode=CitedFullRecord&isickref=WOS:000310882500015.
[61] 王大成. Modeling studies with Helicobacter pylori octaprenyl pyrophosphate synthase reveal the enzymatic mechanism of trans-prenyltransferases. INTERNATIONAL JOURNAL OF BIOCHEMISTRY & CELL BIOLOGY[J]. 2012, 44(12): 2116-2123, http://dx.doi.org/10.1016/j.biocel.2012.09.002.
[62] Li, Tang, Zhao, Kexin, Huang, Van, Li, Defeng, Jiang, ChengYing, Zhou, Nan, Fan, Zheng, Liu, ShuangJiang. The TetR-Type Transcriptional Repressor RolR from Corynebacterium glutamicum Regulates Resorcinol Catabolism by Binding to a Unique Operator, rolO. APPLIED AND ENVIRONMENTAL MICROBIOLOGY[J]. 2012, 78(17): 6009-6016, http://dx.doi.org/10.1128/AEM.01304-12.
[63] Gao, Mingming, Li, Defeng, Hu, Yonglin, Zhang, Ying, Zou, Quanming, Wang, DaCheng. Crystal Structure of TNF-alpha-Inducing Protein from Helicobacter Pylori in Active Form Reveals the Intrinsic Molecular Flexibility for Unique DNA-Binding. PLOS ONE[J]. 2012, 7(7): http://www.irgrid.ac.cn/handle/1471x/756757.
[64] Li DeFeng, Zhang JiaYue, Hou Yanjie, Liu Lei, Liu ShuangJiang, Liu Wei. Crystallization and preliminary crystallographic analysis of 2-aminophenol 1,6-dioxygenase complexed with substrate and with an inhibitor. ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY AND CRYSTALLIZATION COMMUNICATIONS[J]. 2012, 68(Pt 11): 1337-1340, http://www.irgrid.ac.cn/handle/1471x/756758.
[65] Zhang, Jinyong, Zhang, Xiaoli, Wu, Chao, Lu, Dongshui, Guo, Gang, Mao, Xuhu, Zhang, Ying, Wang, DaCheng, Li, Defeng, Zou, Quanming. Expression, Purification and Characterization of Arginase from Helicobacter pylori in Its Apo Form. PLOS ONE[J]. 2011, 6(10): http://www.irgrid.ac.cn/handle/1471x/757515.
[66] 王大成. A Novel Binding Pattern Unique in Two Ligands for One Carbohydrate Recognition Domain in Galectins. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS[J]. 2011, 38(9): 810-815, http://www.irgrid.ac.cn/handle/1471x/757471.
[67] Li, DeFeng, Zhang, Ning, Hou, YanJie, Huang, Yan, Hu, Yonglin, Zhang, Ying, Liu, ShuangJiang, Wang, DaCheng. Crystal Structures of the Transcriptional Repressor RoIR Reveals a Novel Recognition Mechanism between Inducer and Regulator. PLOS ONE[J]. 2011, 6(5): http://124.16.144.217/handle/311023/2390.
[68] Fang, Ti, Li, DeFeng, Zhou, NingYi. Identification and clarification of the role of key active site residues in bacterial glutathione S-transferase zeta/maleylpyruvate isomerase. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS[J]. 2011, 410(3): 452-456, http://www.irgrid.ac.cn/handle/1471x/757318.
[69] Zhang, Jinyong, Zhang, Xiaoli, Mao, Xuhu, Zou, Quanming, Li, Defeng. Expression, crystallization and preliminary crystallographic study of octaprenyl pyrophosphate synthase from Helicobacter pylori. ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS[J]. 2011, 67: 263-265, https://www.webofscience.com/wos/woscc/full-record/WOS:000287030600020.
[70] Zhang, Jinyong, Zhang, Xiaoli, Mao, Xuhu, Zou, Quanming, Li, Defeng. Crystallization and preliminary crystallographic studies of Helicobacter pylori arginase. ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS[J]. 2011, 67: 707-709, https://www.webofscience.com/wos/woscc/full-record/WOS:000291216800018.
[71] BIAN ChengFeng, ZHANG Ying, LI DeFeng, WANG DaCheng. 半乳糖凝集素糖结合的新模式:一个糖结合域的双重配体结合. 生物化学与生物物理进展[J]. 2011, 38(9): 810-815, http://lib.cqvip.com/Qikan/Article/Detail?id=39403857.
[72] Bian, ChengFeng, Zhang, Ying, Sun, Hui, Li, DeFeng, Wang, DaCheng. Structural Basis for Distinct Binding Properties of the Human Galectins to Thomsen-Friedenreich Antigen. PLOS ONE[J]. 2011, 6(9): http://www.irgrid.ac.cn/handle/1471x/757465.
[73] 王大成. Crystal Structures of a Populus tomentosa 4-Coumarate:CoA Ligase Shed Light on Its Enzymatic Mechanisms. PLANT CELL[J]. 2010, 22(9): 3093-3104, http://www.irgrid.ac.cn/handle/1471x/757174.
[74] Zhang, Ning, Ren, Xiaoming, Zhu, Deyu, Li, Defeng, Wang, Dacheng. Crystallization and preliminary crystallographic studies of CorC, a magnesium-ion transporter. ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS[J]. 2010, 66: 681-683, https://www.webofscience.com/wos/woscc/full-record/WOS:000278165900013.
[75] 王大成. Crystal structure of human programmed cell death 10 complexed with inositol-(1,3,4,5)-tetrakisphosphate: A novel adaptor protein involved in human cerebral cavernous malformation. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS[J]. 2010, 399(4): 587-592, http://dx.doi.org/10.1016/j.bbrc.2010.07.119.
[76] 王大成. A nuclear ligand MRG15 involved in the proapoptotic activity of medicinal fungal galectin AAL (Agrocybe aegerita lectin). BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS[J]. 2010, 1800(4): 474-480, http://dx.doi.org/10.1016/j.bbagen.2010.01.010.
[77] 王大成. Structural insights into the recognition mechanism between an antitumor galectin AAL and the Thomsen-Friedenreich antigen. FASEB JOURNAL[J]. 2010, 24(10): 3861-3868, http://www.irgrid.ac.cn/handle/1471x/757195.
[78] 王大成. Structural Basis for the Tumor Cell Apoptosis-Inducing Activity of an Antitumor Lectin from the Edible Mushroom Agrocybe aegerita. JOURNAL OF MOLECULAR BIOLOGY[J]. 2009, 387(3): 694-705, http://dx.doi.org/10.1016/j.jmb.2009.02.002.
[79] 王大成. Importance of nuclear localization for the apoptosis-induced activity of a fungal galectin AAL (Agrocybe aegerita lectin). BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS[J]. 2009, 386(3): 437-442, http://www.irgrid.ac.cn/handle/1471x/757814.
[80] 王大成. Crystal structure of Mabinlin II: A novel structural type of sweet proteins and the main structural basis for its sweetness. JOURNAL OF STRUCTURAL BIOLOGY[J]. 2008, 162(1): 50-62, http://dx.doi.org/10.1016/j.jsb.2007.12.007.

科研活动

   
科研项目
( 1 ) 镁离子转运蛋白CorC受腺苷类配体以及镁离子调控的分子机理的生物化学和结构生物学研究, 主持, 国家级, 2013-01--2016-12
( 2 ) 芳香烃降解相关的调控蛋白NCgl1110以及其与芳香烃配体、核酸复合物的晶体结构和功能研究, 主持, 国家级, 2009-01--2011-12
( 3 ) 重要病原微生物感染与耐药性相关的膜蛋白结构功能研究, 主持, 国家级, 2013-01--2017-12
( 4 ) 结核病耐药相关的基础和应用研究, 主持, 部委级, 2013-01--2015-12
( 5 ) 重要生命活动中关键膜蛋白及蛋白质复合物的结构与功能研究, 参与, 国家级, 2011-01--2015-12
( 6 ) 微生物结构生物学, 主持, 市地级, 2018-02--2020-02
( 7 ) 微生物趋化运动和运动调控的分子机制, 主持, 市地级, 2018-01--2020-12
( 8 ) 趋化受体MCP2201介导的趋化激活和抑制的机理研究, 主持, 国家级, 2019-01--2022-12
参与会议
(1)邻氨基苯酚-1,6-双加氧酶APD和水杨酸-5-加羟酶NagGH的比较研究   第二十一次全国环境微生物学学术研讨会   李德峰   2018-12-07
(2)Structural studies of bacterial transmembrane chemoreceptor MCP2201   中德微生物趋化、环境污染物的生物降解与污染环境修复双边研讨会   2016-10-16

指导学生

现指导学生

韩群  硕士研究生  085238-生物工程  

秦亚玲  博士研究生  071010-生物化学与分子生物学