基本信息
袁辉明  男  硕导  中国科学院大连化学物理研究所
电子邮件: huimingyuan@dicp.ac.cn
通信地址: 大连市中山路457号
邮政编码:

招生信息

   
招生专业
070302-分析化学
招生方向
单细胞蛋白质组学分析
外泌体纯化制备与质量控制评价
蛋白质组样品处理仪器创制

教育背景

2003-09--2010-01 中国科学院大连化学物理研究所 博士
1999-09--2003-07 江西师范大学 学士

工作经历

   
工作简历
2011-10--今 中国科学院大连化学物理研究所 副研究员
2010-01--2011-09 中国科学院大连化学物理研究所 助理研究员

专利与奖励

   
专利成果
( 1 ) 集蛋白质收集和酶解于一体的样品预处理装置及其制备, 发明, 2012, 第 2 作者, 专利号: CN101470052B
( 2 ) 一种微液相分离系统与质谱联用的多通道电喷雾离子源, 发明, 2012, 第 4 作者, 专利号: CN101206207B
( 3 ) 低残留无机-有机杂化整体基质固定化酶反应器及其制备, 发明, 2012, 第 2 作者, 专利号: 201210555716.9
( 4 ) 一种生物样品溶液中外泌体富集方法, 发明, 2019, 第 3 作者, 专利号: 201911226414.5
( 5 ) 一种利用滤膜实现外泌体富集的装置和外泌体富集方法, 发明, 2019, 第 4 作者, 专利号: 201911226412.6
( 6 ) 一种烟气体液代谢糖苷衍生物集成化样品处理系统及应用, 发明, 2019, 第 2 作者, 专利号: 201911229401.3
( 7 ) METHOD FOR PRETREATING PROTEIN IN EX VIVO BODY FLUID, 发明, 2019, 第 2 作者, 专利号: 16462464
( 8 ) HIGH THROUGHPUT BODY FLUID PROTEIN SAMPLE PREPARATION DEVICE AND APPLICATIONS THEREOF, 发明, 2019, 第 2 作者, 专利号: 16462451
( 9 ) 一种外泌体纯化的装置及应用, 发明, 2020, 第 3 作者, 专利号: 202011373189.0
( 10 ) 一种海洋微生物蛋白质组的预处理方法, 发明, 2020, 第 5 作者, 专利号: 202011377686.8
( 11 ) 一种巯基化多肽的富集方法, 发明, 2020, 第 2 作者, 专利号: 201510902151.0
( 12 ) 一种硫巯化翻译后修饰肽段的定性与定量方法, 发明, 2020, 第 3 作者, 专利号: 201611020027.2
( 13 ) 富集材料及制备和生物样品溶液中细胞外囊泡的富集方法, 发明, 2021, 第 3 作者, 专利号: 202110670403.7
( 14 ) 一种蛋白质组样品处理方法, 发明, 2021, 第 2 作者, 专利号: 202120664500.5

出版信息

   
发表论文
(1) Fully integrated protein absolute quantification platform for analysis of multiple tumor markers in human plasma, Talanta, 2021, 通讯作者
(2) Integrated proteomic sample preparation with combination of on-line high-abundance protein depletion, denaturation, reduction, desalting and digestion to achieve high throughput plasma proteome quantification, Analytica Chimica Acta, 2021, 通讯作者
(3) Fully automated sample treatment method for high throughput proteome analysis, Science China-Chemistry, 2021, 第 1 作者
(4) Comprehensive Analysis of Protein N-Terminome by Guanidination of Terminal Amines, Analytical Chemistry, 2020, 第 6 作者
(5) Advances in Exosome Isolation Methods and Their Applications in Proteomic Analysis of Biological Samples, Analytical and bioanalytical chemistry, 2019, 通讯作者
(6) Recent Advances in Multidimensional Separation for Proteome Analysis, Analytical chemistry, 2019, 第 1 作者
(7) Site-Specific Quantification of Persulfidome by Combining an Isotope-Coded Affinity Tag With Strong Cation-Exchange-Based Fractionation, Analytical chemistry, 2019, 通讯作者
(8) Integrated Platform With Combination of On-Line Protein Digestion, Isotope Dimethyl Labeling and Multidimensional Peptide Separation for High-Throughput Proteome Quantification, Analytica Chimica Acta, 2018, 第 2 作者
(9) Enzymatic Reactor with Trypsin Immobilized on Graphene Oxide Modified Polymer Microspheres To Achieve Automated Proteome Quantification, Analytical chemistry, 2017, 第 1 作者
(10) Depletion of internal peptides by site-selective blocking, phosphate labeling, and TiO2 adsorption for in-depth analysis of C-terminome, Analytical and bioanalytical chemistry, 2016, 第 4 作者
(11) Preparation of hydrophilic monolithic capillary column by in situ photo-polymerization of N-vinyl-2-pyrrolidinone and acrylamide for highly selective and sensitive enrichment of N-linked glycopeptides, Talanta, 2016, 第 2 作者
(12) Particulate capillary precolumns with double-end polymer monolithic frits for on-line peptide trapping and preconcentration, CHINESE CHEMICAL LETTERS, 2015, 第 2 作者
(13) Integrated SDS removal and protein digestion by hollow fiber membrane based device for SDS-assisted proteome analysis, Talanta, 2015, 第 2 作者
(14) Integrated platform with a combination of online digestion and O-18 labeling for proteome quantification via an immobilized trypsin microreactor, Analyst, 2015, 第 2 作者
(15) Preparation of high efficiency and low carry-over immobilized enzymatic reactor with methacrylic acid-silica hybrid monolith as matrix for on-line protein digestion, Journal of Chromatography A, 2014, 第 1 作者
(16) 1-Dodecyl-3-Methylimidazolium Chloride-Assisted Sample Preparation Method for Efficient Integral Membrane Proteome Analysis, Analytical chemistry, 2014, 第 4 作者
(17) An integrated sample pretreatment platform for quantitative N-glycoproteome analysis with combination of on-line glycopeptide enrichment, deglycosylation and dimethyl labeling, Analytica Chimica Acta, 2014, 第 6 作者
(18) Improved accuracy for label-free absolute quantification of proteome by combining the absolute protein expression profiling algorithm and summed tandem mass spectrometric total ion current, Analyst, 2014, 第 5 作者
(19) Biphasic Microreactor for Efficient Membrane Protein Pretreatment with a Combination of Formic Acid Assisted Solubilization, On-Column pH Adjustment, Reduction, Alkylation, and Tryptic Digestion, Analytical chemistry, 2013, 第 3 作者
(20) An activity-maintaining sequential protein extraction method for bioactive assay and proteome analysis of velvet antlers, Talanta, 2013, 第 2 作者
(21) A hydrophilic immobilized trypsin reactor with N-vinyl-2-pyrrolidinone modified polymer microparticles as matrix for highly efficient protein digestion with low nonspecific adsorption, Journal of Chromatography A, 2012, 第 2 作者
(22) Integrated Platform for ProteomeProfiling with Combination of Microreversed Phase Based Protein and Peptide Separation via Online Solvent Exchange and Protein Digestion, Analytical Chemistry, 2012, 第 1 作者
(23) Recent advances on multidimensional liquid chromatography-mass spectrometry for proteomics: From qualitative to quantitative analysis-A review, Analytica Chimica Acta, 2012, 第 2 作者
(24) Integrated Sample Pretreatment System for N-Linked Glycosylation Site Profiling with Combination of Hydrophilic Interaction Chromatography and PNGase F Immobilized Enzymatic Reactor via a Strong Cation Exchange Precolumn , Analytical Chemistry, 2011, 第 3 作者
(25) Integrated platform of capillary isoelectric focusing, trypsin immobilized enzyme microreactor and nanoreversed-phase liquid chromatography with mass spectrometry for online protein profiling , Electrophoresis, 2011, 第 4 作者
(26) On-line combination of monolithic immobilized pH gradient-based capillary isoelectric focusing and capillary zone electrophoresis via a partially etched porous interface for protein analysis, Journal of Chromatography B, 2011, 第 5 作者
(27) Weak anion and cation exchange mixed-bed microcolumn for protein separation , Journal of Separation Science, 2010, 第 2 作者
(28) Integrated Platform for Proteome Analysis with Combination of Protein and Peptide Separation via Online Digestion , Analytical Chemistry, 2009, 第 1 作者
(29) Integrated protein analysis platform based on column switch recycling size exclusion chromatography, microenzymatic reactor and mu RPLC-ESI-MS/MS, Journal of Chromatography A, 2009, 第 1 作者
(30) Columns switch recycling size exclusion chromatography for high resolution protein separation , Journal of Chromatography A, 2009, 第 1 作者
(31) 
发表著作
(1) 2.18 整体柱,样品预处理的原理,Comprehensive Sampling and Sample Preparation:2.18 Monoliths, fundamentals for sample preparation,Elsevier,1900-01,第1作者

科研活动

   
科研项目
( 1 ) 用于膜蛋白质组样品高通量预处理的串级膜接口与平行酶反应器的集成化方法研究, 主持, 国家级, 2012-01--2014-12
( 2 ) 膜蛋白质样品高通量分析方法的建立及应用研究, 主持, 市地级, 2011-01--2013-12
( 3 ) 肿瘤转移预警的蛋白质组分离鉴定新技术新方法研究, 参与, 国家级, 2010-01--2013-12
( 4 ) 蛋白质定量新方法及相关技术研究, 参与, 国家级, 2013-01--2017-12
( 5 ) 在线蛋白质预处理系统, 主持, 国家级, 2012-07--2017-03
( 6 ) 基于液质联用的单细胞分泌蛋白质组定量分析新方法, 主持, 国家级, 2020-01--2023-12
( 7 ) 深度覆盖的蛋白质组精准鉴定与定量新技术, 参与, 国家级, 2017-07--2022-06
( 8 ) 医学生命组学数据质量控制关键技术研发与应用示范, 参与, 国家级, 2018-07--2020-12
( 9 ) 细胞中重要调控蛋白质的发现和原位相互作用的分析新方法, 参与, 国家级, 2020-01--2024-12
参与会议
(1)Preparation of methacrylic acid modified silica hybrid monolith based immobilized enzymatic reactor with “one-pot” approach for on line protein digestion with low protein carry-over   Yuan Huiming   2012-02-11
(2)An Integrated Protein Pretreatment Device as the Interface of Two-dimensional High Performance Liquid Chromatography System for High Throughput Human Serum Proteome Analysis   Yuan Huiming   2011-10-08
(3)An integrated device with combination of protein denaturation, reduction, desalting and on-column digestion for high throughput pretreatment of proteins    Yuan Huiming   2011-04-15