基本信息
Dimitri LAVILLETTE  男  博导  中国科学院上海巴斯德研究所
电子邮件: dlaville@ips.ac.cn
通信地址: 岳阳路320号新生命科学大楼B座A326
邮政编码: 200031

研究领域

近20年,爆发了一些可同时感染人和牲畜的新生、再生病毒性流行病,其中很大一部分是由吸血的节肢动物叮咬脊椎动物造成病毒(虫媒病毒)感染引起的。虫媒病毒分为黄病毒科(Flaviviridae)和披膜病毒科(Togaviridae)等。登革热病毒(Dengue Virus)就是由蚊虫传播的黄病毒科的虫媒病毒。最近的研究显示,每年约有4亿人感染登革热病毒,还有25亿人处于接触登革热病毒的高风险之中。大部分的登革热病毒感染表现为轻症并可以自愈,但仍有部分病例会发展成严重的登革出血热(DHF)/登革休克综合症(DSS)甚至死亡。另一种重要的虫媒病毒是披膜病毒科、甲病毒属(Alphavirus) 的基孔肯雅病毒(Chikungunya Virus)。从2005年起,基孔肯雅病毒通过新的载体,白纹伊蚊,扩大了其传播范围,甚至传播到了加勒比海地区和美洲大陆。这两种虫媒病毒给公共卫生和安全带来了巨大的威胁,但目前还没有针对性的疫苗和药物,关于这些病毒在细胞和分子水平上的细胞嗜性和致病机理更是存在很多未知。我们以这两种虫媒病毒为研究对象,感染哺乳动物细胞和蚊虫,通过体内外实验,揭示病毒致病因子、宿主促病毒因子或者限制性因子。特别关注i)在病毒进入细胞、跨种传播和易感性中发挥重要作用的宿主因子ii)蚊虫体内影响病毒复制和传播的细胞反应和信号通路。这些研究将有利于研发防治虫媒病毒的新策略。           

招生信息

   
招生专业
071005-微生物学
071009-细胞生物学
085238-生物工程
招生方向
登革热、基孔肯雅病毒和寨卡病毒致病的细胞机理
登革热、基孔肯雅病毒和寨卡病毒疫苗和特效药的开发
其他新发病毒Co-infection和virus-virus interaction相关研究

教育背景

2009-02--2009-12   里昂高等师范学院ENS de Lyon (Normal Superior School)   Habilitation to Direct Research (HDR)
1997-10--2000-11   法国国家医学与健康科学研究院 ENS de Lyon U412 INSERM, France   博士 PhD
1994-09--1997-10   法国里昂大学 Lyon University, France   硕士 Master
1991-09--1994-09   法国第戎大学 Dijon University, France   学士 Bachelor
学历
2009-12 里昂高等师范学院ENS de Lyon (Normal Superior School) Habilitation to Direct Research (HDR) 
2000-11 法国国家医学与健康科学研究院 ENS de Lyon U412 INSERM, France 博士 PhD
学位
2000-11 法国国家医学与健康科学研究院 ENS de Lyon U412 INSERM, France 博士 PhD

工作经历

   
工作简历
2014-11~现在, 中科院上海巴斯德研究所 Institut Pasteur of Shanghai, CAS, 研究员 Principal investigators
2012-11~2014-11,法国国家科学研究院 CNRS, France, 副教授 Tenure staff scientist position(CNRS CR1)
2011-01~2011-12,牛津大学Glycobiology Institute. Oxford (UK), 访问学者 Visiting scientist
2003-05~2012-11,法国国家医学与健康研究院 INSERM U758, ENS de Lyon, France, 副教授 Tenure staff scientist position(CNRS CR1)
2001-02~2003-05,美国俄勒冈卫生科学大学 OHSU, Portland,USA, 博士后 Post Doc
2000-11~2001-02,美国Mayo医学中心 Mayo Clinic, Minnesota, USA, 项目访问学者 Visiting scientist
社会兼职
2010-01-01-今,Treasurer and Active Member of the Advisory Council of the European Society for Virology , Treasurer and Active Member
2010-01-01-今,Member of the scientific council of FINOVI foundation , Member of the scientific council

教授课程

   

专利与奖励

   
奖励信息
(1) 第五批上海“千人计划”引进人才, 一等奖, 省级, 2015
(2) 中国科学院2015年度第二批“百人计划”A类, 院级, 2015
专利成果
( 1 ) LENTIVIRAL VECTORS PSEUDOTYPED WITH MUTANT BaEV GLYCOPROTEINS, 发明, 2012, 第 3 作者, 专利号: EP2761010
( 2 ) Procedimiento de detección de la expresión de una proteína de cubierta de un retrovirus endógeno humano y utilizaciones de un gen que codifica para esta proteína, 发明, 2000, 第 4 作者, 专利号: es2444649
( 3 ) METHOD FOR DETECTING ENVELOPE PROTEIN EXPRESSION OF HUMAN ENDOGENOUS RETROVIRUS, AND USE OF GENE ENCODING THE SAME PROTEIN, 发明, 2008, 第 4 作者, 专利号: JP2009072194
( 4 ) Method for detecting the expression of an envelope protein of a human endogenous retrovirus and uses of a gene coding for said protein, 发明, 2000, 第 4 作者, 专利号: US7442550
( 5 ) METHODS FOR IDENTIFYING RECEPTOR UP- AND DOWNMODULATORS, 发明, 2002, 第 4 作者, 专利号: WO2002095400
( 6 ) METHOD FOR DETECTING THE EXPRESSION OF AN ENVELOPE PROTEIN OF A HUMAN ENDOGENOUS RETROVIRUS AND USES OF A GENE CODING FOR SAID PROTEIN, 发明, 2000, 第 4 作者, 专利号: EP1212359
( 7 ) METHOD FOR DETECTING THE EXPRESSION OF AN ENVELOPE PROTEIN OF A HUMAN ENDOGENOUS RETROVIRUS AND USES OF A GENE CODING FOR SAID PROTEIN, 发明, 2000, 第 5 作者, 专利号: WO2001016171
( 8 ) NEW POLYPEPTIDES AND THEIR USE FOR THE RESCUE OF FUSION DEFECTIVE VIRUS OR RETROVIRUS GLYCOPROTEINS, 发明, 2000, 第 3 作者, 专利号: WO2000071578
( 9 ) USE OF A PROLINE-RICH SEQUENCE TO IMPROVE THE FUSOGENIC CHARACTER OF RETROVIRAL ENVELOPES, 发明, 1999, 第 2 作者, 专利号: CA2317026
( 10 ) USE OF A PROLINE-RICH SEQUENCE TO IMPROVE THE FUSOGENIC CHARACTER OF RETROVIRAL ENVELOPES, 发明, 1999, 第 2 作者, 专利号: WO1999036561
( 11 ) CHIMERIC VIRUS -LIKE PARTICLES (VLP) CONTAINING FUNCTIONAL HMPV PROTEINS, 发明, 2013, 第 5 作者, 专利号: WO2013132107
( 12 ) Chimeric virus-like particles (VLP) containing functional hMPV proteins, 发明, 2012, 第 4 作者, 专利号: EP2636413
( 13 ) LENTIVIRAL VECTORS PSEUDOTYPED WITH MUTANT BaEV GLYCOPROTEINS, 发明, 2012, 第 3 作者, 专利号: WO2013045639
( 14 ) Procedimiento de detección de la expresión de una proteína de cubierta de un retrovirus endógeno humano y utilizaciones, 发明, 2012, 第 4 作者, 专利号: es2376726
( 15 ) Method for detecting the expression of a human endogenous retrovirus envelope protein and uses of a coding gene for said protein, 发明, 2000, 第 4 作者, 专利号: EP2385058
( 16 ) METHODS FOR ENHANCING THE POTENCY OF HCV NEUTRALIZING ANTIBODIES, 发明, 2000, 第 3 作者, 专利号: WO2006103493
( 17 ) USE OF A PROLINE-RICH SEQUENCE TO IMPROVE THE FUSOGENIC CHARACTER OF RETROVIRAL ENVELOPES, 发明, 1999, 第 2 作者, 专利号: EP1047791
( 18 ) LENTIVIRAL VECTORS PSEUDOTYPED WITH MUTANT BaEV GLYCOPROTEINS, 发明, 2012, 第 3 作者, 专利号: US20140235700
( 19 ) LENTIVIRAL VECTORS PSEUDOTYPED WITH MUTANT BaEV GLYCOPROTEINS, 发明, 2014, 第 3 作者, 专利号: US20140235700
( 20 ) LENTIVIRAL VECTORS PSEUDOTYPED WITH MUTANT BaEV GLYCOPROTEINS, 发明, 2014, 第 3 作者, 专利号: EP2761010
( 21 ) Procedimiento de detección de la expresión de una proteína de cubierta de un retrovirus endógeno humano y utilizaciones de un gen que codifica para esta proteína, 发明, 2014, 第 4 作者, 专利号: es2444649

出版信息

   
发表论文
(1) A protein coevolution method uncovers critical features of the Hepatitis C Virus fusion mechanism., PLoS Pathog, 2018, 通讯作者
(2) Chronic infection of mosquito by Chikungunya virus increases its susceptibility to Zika, Dengue and Yellow fever viruses during coinfection., Int.J.Mol.Sci, 2017, 通讯作者
(3) Specialization of hepatitis C virus envelope glycoproteins for B-lymphocytes in chronically infected patients., J Virol., 2016, 通讯作者
(4) New insights into the understanding of hepatitis C virus entry and cell-to-cell transmission by using the ionophore Monensin A, J VIROL (IF2014 4.43), 2015, 通讯作者
(5) The sheep tetherin paralog oBST2B blocks envelope glycoprotein incorporation into nascent retroviral virions., J VIROL (IF2014 4.43), 2015, 通讯作者
(6) Low cross-neutralization of hepatitis C correlates with liver disease in immunocompromized patients,  AIDS (IF2013 6.55), 2015, 通讯作者
(7) The mechanism of HCV entry into host cells., PROG MOL BIOL TRANSL SCI. (IF2013 3.11), 2015, 第 2 作者
(8) A Critical Interaction Between E1 and E2 Glycoproteins Determines Binding and Fusion Properties of Hepatitis C Virus During Cell Entry, HEPATOLOGY (IF2012 12.03), 2014, 通讯作者
(9) Baboon envelope pseudotyped lentiviral vectors outperform VSV-G pseudotyped lentiviral vectors for gene transfer into cytokine-stimulated and resting hematopoietic stem cells, BLOOD(IF2012 9), 2014, 通讯作者
(10) The Expression of the Hepatocyte SLAMF3 (CD229) Receptor Enhances the Hepatitis C Virus Infection, PLOS ONE(IF2013 3.53), 2014, 通讯作者
(11) Up-Regulation of the ATP-Binding Cassette Transporter A1 Inhibits Hepatitis C Virus Infection., PLOS ONE(IF2013 3.53), 2014, 通讯作者
(12) Virus-like particle vaccine induces cross-protection against human metapneumovirus infections in mice., VACCINE (IF2011 3.458), 2013, 通讯作者
(13) The antimalarial ferroquine is an inhibitor of hepatitis C virus, HEPATOLOGY (IF2012 12.00), 2013, 通讯作者
(14) Characterization of hepatitis C virus particle sub-populations reveals multiple usage of the scavenger receptor BI for entry steps, J BIOL CHEM (IF2011 5.11), 2012, 通讯作者
(15) Matrigel-embedded 3D culture of Huh-7 cells as a hepatocyte-like polarized system to study hepatitis C virus cycle. , VOROLOGY (IF2011 3.35), 2012, 通讯作者
(16) Distinct roles in folding, CD81 receptor binding and viral entry for conserved histidine residues of hepatitis C virus glycoprotein E1 and E2. , BIOCHEMICAL JOURNAL (IF2011 4.89), 2012, 通讯作者
(17) A Prime-Boost Strategy Using Virus-Like Particles Pseudotyped for HCV Proteins Triggers Broadly Neutralizing Antibodies in Macaques., SCIENCE TRANSLATIONAL MEDICINE (IF2010 7.18), 2011, 通讯作者
(18) EGFR and EphA2 are host factors for hepatitis C virus entry and possible targets for antiviral therapy. , NATURE MEDICINE (IF2010 25.43), 2011, 通讯作者
(19) Mechanism of Inhibition of Enveloped Virus Membrane Fusion by the Antiviral Drug Arbidol., PLOS ONE (IF2010 4.41), 2011, 第 4 作者
(20) Cell Entry of Enveloped Viruses, ADVANCES IN GENETICS (IF2011 3.77), 2011, 第 2 作者
(21) Kinases required in hepatitis C virus entry and replication highlighted by small interference RNA screening, FASEB J. (IF2009 6.40), 2009, 通讯作者
(22) Characterization of fusion determinants points to the involvement of three discrete regions of both E1 and E2 glycoproteins in the membrane fusion process of hepatitis C virus, J VIROL. (IF2006 5.34), 2007, 第 1 作者
(23) Human serum facilitates hepatitis C virus infection, and neutralizing responses inversely correlate with viral replication kinetics at the acute phase of hepatitis C virus infection, J VIROL. (IF2005 5.1), 2005, 第 1 作者
(24) Characterization of host-range and cell entry properties of the major genotypes and subtypes of hepatitis C virus, HEPATOLOGY (IF2005 9.8), 2005, 第 1 作者
发表著作
(1) 细胞融合:调节和控制 http://www.amazon.cn/Cell-Fusions-Regulation-and-Control/dp/9048197716, Cell Fusions: Regulation and Control .A Comparative Portrait of Retroviral Fusogens and Syncytins. ,  Springer; 1 , 2010-11, 第 3 作者
(2) HCV进入宿主细胞的机制, The mechanism of HCV entry into host cells. , Progress in Molecular Biology and Translational Science--Elsevier, 2014-12, 第 2 作者

科研活动

   
科研项目
( 1 ) 中国科学院百人计划A类:Virus-host interactions at the cell level, 主持, 部委级, 2016-01--2018-12
( 2 ) 寨卡病毒感染致病机制及综合防控, 主持, 国家级, 2017-10--2019-12
参与会议
(1)“Interaction between Dengue, Zika and Chikungunya Viruses during Coinfection in Mosquito”    2017-09-26
(2) “The evolution and consequences of Zika virus post translational modifications”   2017-06-16
(3)Zika in China, infection cell entrance and molecular structures   2004年中法防治新发传染病合作协议的指导委员会会议   2016-07-01
(4)Modulation of Zika tropism in insect and mammal cells, Zika   国际Zika 交流会议   2016-06-01
(5)Strong specialization of HCV envelope glycoproteins is associated with the establishment of B-lymphocyte reservoirs in chronically infected patients.   第十四次全国肝炎会仪   F Douam, LM Bobay, G Maurin, J Fresquet, FL Cosset, C Féray et D Lavillette   2014-01-23
(6)Identification of functional networks in E1E2 glycoproteins unveils Hepatitis C virus fusion mechanisms and new therapeutic opportunities.    第五届欧洲国会病毒学会议   F Douam, L Dib, VL Dao Thi, G Maurin, L Schwaller, J Fresquet, D Mompelat, FL Cosset, A Carbone, D Lavillette.   2013-09-11
(7)Cell entry process of in vitro and in vivo produced hepatitis C virus (HCV)   第五届欧洲国会病毒学会议    S.CALATTINI, L.DAO-THI, F. FUSIL, J. MANCIP, D. LAVILLETTE, F.L. COSSET*, M. DREUX*   2013-09-11
(8)Baboon retrovirus envelope pseudotyped LVs allow efficient transduction of progenitor T cells, thymocytes and adult T and B cells.   第五届欧洲国会病毒学会议    Anais GIRARD, Fouzia AMIRACHE, Caroline COSTA, Camille LEVY, Dimitri LAVILLETTE, Didier NEGRE, Francois-Lo?c COSSET and Els VERHOEYEN.   2013-09-11
(9)Association d’approches expérimentales et bioinformatiques pour déterminer le r?le fonctionnel de domaines qui co-évoluent au sein de protéines virales: application aux glycoprotéines d’enveloppe du virus de l’hépatite C.   第十五届全法语病毒会议   F Douam, VL Dao Thi, G Maurin, J Fresquet, D Mompelat, FL Cosset and D Lavillette   2013-04-18
(10)Active role of E1 in the conformation of E1E2 and in the exposition of receptor binding domains.    第十二次全国肝炎会议   Florian Douam, Guillemette Maurin, Judith Fresquet, Fran?ois-Lo?c Cosset and Dimitri Lavillette.    2013-01-24
(11)Prevention from Chikungunya virus experimental infection in macaques by live attenuated and replicating vaccine.   第六届欧洲动物传播病毒会议   Lavillette D, Dereuddre-Bosquet N, Lotteau V, Cosset FL, Roques P, Le Grand R.   2011-10-01
(12)Active role of E1 in the conformation of E1E2 and in the exposition of receptor binding domains.    第十八届丙肝及相关病毒的国际研讨会   F Douam, G Maurin, J Fresquet, FL Cosset and D Lavillette   2011-09-08
(13)Identification of an interaction between the N-terminal and the transmembrane domains of E1 envelope glycoprotein important for HCV cell entry process. Mutations in SR-BI or in HCV-E2 that abrogate SR-BI/H    第十七届丙肝及相关病毒的国际研讨会   Guillemette Maurin, Judith Fresquet, Ophelia Granio, Fran?ois-Lo?c Cosset, Dimitri Lavillette   2010-09-10

合作情况

   
项目协作单位
   

指导学生

现指导学生

黄鹂  硕士研究生  085238-生物工程  

段博文  博士研究生  071005-微生物学