光合作用与胁迫信号

Ever since the endosymbiotic transfer of plastid genes to the host genome, the development, maintenance and metabolic activity of the plastid have been largely dependent on coordinated gene expression between the host and the endosymbiont, despite the fact that they are physically and biochemically distinct. As the crucial metabolic processes of photosynthesis and photorespiration as well as generation of myriad of molecules including amino acid, secondary metabolite, fatty acid, chemical energy and plant hormones take place in plastid, plastid-to-nucleus retrograde signaling has indispensably evolved. This retrograde communication is essential not only for the biogenesis of plastid but also to integrate a multitude of environmental signals into responses assisting in the fine-tuning of plant development.Hence, a rapid adjustment of plastid homeostasis is possible with the communication, which eventually becomes vital for plants to acclimate under the fluctuating environment. 

The primary research themes in the Kim laboratory are perception/responding mechanism of chloroplast to stress, chloroplast-to-nucleus signaling, and chloroplast-mediated stress response including growth inhibition, acclimation and cell death. In addition, we will try to understand the mechanism that underlies chloroplast-mitochondria communication and chloroplast-dependent epigenetic inheritance. In this context, we may eventually understand how chloroplast acts as sensor of multitude of environmental factors and affects plant growth and productivity. The research approach utilizes genetic screen, deep sequencing, proteomics, 540 chloroplast homozygous mutant lines, metabolomics, cell biology and biochemistry in conjunction with plant physiology. 

   

071010-生物化学与分子生物学

逆境—光合作用及信号抗逆

2000-05--2004-07   瑞士联邦理工学院，苏黎世（ETH Zurich, Swiss Federal Institute of Technology）   博士
1988-03--1998-02   韩国东国大学（Dongguk University, Korea）   学士和硕士

研究生

博士

2020.5-至今：中国科学院分子植物科学卓越创新中心，研究员

2014.8- 2020.4：  中国科学院上海生命科学研究院，上海植物逆境生物学中心，研究员

2008.4- 2014.7：博伊斯汤普森研究所，康奈尔大学（美国），研究助理

2006.7- 2008.3：瑞士苏黎世联邦理工学院，研究组长

2004.7- 2006.7：瑞士苏黎世联邦理工大学，博士后，导师：Klaus Apel教授

2020-05~现在, 中国科学院分子植物科学卓越创新中心, 研究员
2014-08~2020-04,中国科学院上海植物逆境生物学研究中心, 研究员
2008-04~2014-07,美国康奈尔大学—鲍依斯•汤普森植物研究所, 研究员
2006-08~2008-03,瑞士联邦理工学院，苏黎世（ETH Zurich, Swiss Federal Institute of Technology）, 研究课题组长
2004-08~2006-07,瑞士联邦理工学院，苏黎世（ETH Zurich, Swiss Federal Institute of Technology）, 博士后
1998-03~2000-02,浦项科技大学, 研究助理

2019-12-31-今,The Plant Cell Guest editor, Guest editor

植物生理与分子生物学
逆境生物学

   

[1] Keun Pyo Lee, Mengping Li, Mengshuang Li, Kaiwei Liu, Kim, Chanhong. Hierarchical regulatory module GENOMES UNCOUPLED1-GOLDEN2-LIKE1/2-WRKY18/40 modulates salicylic acid signaling. Plant Physiology[J]. 2023, [2] Liu, Kaiwei, Lee, Keun Pyo, Duan, Jianli, Kim, Eun Yu, Singh, Rahul Mohan, Di, Minghui, Meng, Zhuoling, Kim, Chanhong. Cooperative role of AtRsmD and AtRimM proteins in modification and maturation of 16S rRNA in plastids. PLANT JOURNAL. 2023, http://dx.doi.org/10.1111/tpj.16135.
[3] Fang, Jun, Li, Bingqi, Chen, LihJen, Dogra, Vivek, Luo, Shengji, Wu, Wangpin, Wang, Pengcheng, Hwang, Inhwan, Li, HsouMin, Kim, Chanhong. TIC236 gain-of-function mutations unveil the link between plastid division and plastid protein import. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA[J]. 2022, 119(11): http://dx.doi.org/10.1073/pnas.2123353119.
[4] Li, Mengping, Lee, Keun Pyo, Liu, Tong, Dogra, Vivek, Duan, Jianli, Li, Mengshuang, Xing, Weiman, Kim, Chanhong. Antagonistic modules regulate photosynthesis-associated nuclear genes via GOLDEN2-LIKE transcription factors. PLANT PHYSIOLOGY[J]. 2022, 188(4): 2308-2324, http://dx.doi.org/10.1093/plphys/kiab600.
[5] Dogra, Vivek, Singh, Rahul Mohan, Li, Mengping, Li, Mingyue, Singh, Somesh, Kim, Chanhong. EXECUTER2 modulates the EXECUTER1 signalosome through its singlet oxygen-dependent oxidation. MOLECULAR PLANT[J]. 2022, 15(3): 438-453, http://lib.cqvip.com/Qikan/Article/Detail?id=7107660069.
[6] Luo, Shengji, Kim, Chanhong. Current Understanding of Temperature Stress-Responsive Chloroplast FtsH Metalloproteases. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES[J]. 2021, 22(22): http://dx.doi.org/10.3390/ijms222212106.
[7] Qingfeng Niu, Zhe Song, Kai Tang, Lixian Chen, Lisi Wang, Ting Ban, Zhongxin Guo, Chanhong Kim, Heng Zhang, ChengGuo Duan, Huiming Zhang, JianKang Zhu, Jiamu Du, Zhaobo Lang. A histone H3K4me1-specific binding protein is required for siRNA accumulation and DNA methylation at a subset of loci targeted by RNA-directed DNA methylation. NATURE COMMUNICATIONS[J]. 2021, 12(1): http://dx.doi.org/10.1038/s41467-021-23637-4.
[8] Laura MedinaPuche, Chanhong Kim, Rosa LozanoDuran, Vivek Dogra. Protocol for evaluating protein relocalization from the plasma membrane to chloroplasts. STAR PROTOCOLS[J]. 2021, 2(4): 100816-, [9] Lv, Xinchen, Zeng, Xiaolin, Hu, Hongmiao, Chen, Lixian, Zhang, Fan, Liu, Rui, Liu, Yue, Zhou, Xuelin, Wang, Changshi, Wu, Zhe, Kim, Chanhong, He, Yuehui, Du, Jiamu. Structural insights into the multivalent binding of the Arabidopsis FLOWERING LOCUS T promoter by the CO-NF-Y master transcription factor complex. PLANT CELL[J]. 2021, 33(4): 1182-1195, http://dx.doi.org/10.1093/plcell/koab016.
[10] Mengping Li, Chanhong Kim. Chloroplast ROS and stress signaling. PLANT COMMUNICATIONS. 2021, 3(1): [11] Li, Bingqi, Fang, Jun, Singh, Rahul Mohan, Zi, Hailing, Lv, Shanshan, Liu, Renyi, Dogra, Vivek, Kim, Chanhong. FATTY ACID DESATURASE5 Is Required to Induce Autoimmune Responses in Gigantic Chloroplast Mutants of Arabidopsis. PLANT CELL[J]. 2020, 32(10): 3240-3255, http://dx.doi.org/10.1105/tpc.20.00016.
[12] Bingqi Li, Jun Fang, Rahul Mohan Singh, Hailing Zi, Shanshan Lv, Renyi Liu, Vivek Dogra, Chanhong Kim. FATTY ACID DESATURASE5 Is Required to Induce Autoimmune Responses in Gigantic Chloroplast Mutants of Arabidopsis. THE PLANT CELL. 2020, 32(10): 3240-3255, [13] CHAN HONG KIM. ROS-Driven Oxidative Modification: Its Impact on Chloroplast-Nucleus Communication.. Front Plant Sci.. 2020, [14] MedinaPuche, Laura, Tan, Huang, Dogra, Vivek, Wu, Mengshi, RosasDiaz, Tabata, Wang, Liping, Ding, Xue, Zhang, Dan, Fu, Xing, Kim, Chanhong, LozanoDuran, Rosa. A Defense Pathway Linking Plasma Membrane and Chloroplasts and Co-opted by Pathogens. CELL[J]. 2020, 182(5): 1109-+, http://dx.doi.org/10.1016/j.cell.2020.07.020.
[15] Wang, Fangfang, Fang, Jun, Guan, Kaoling, Luo, Shengji, Dogra, Vivek, Li, Bingqi, Ma, Demin, Zhao, Xinyan, Pyo Lee, Keun, Sun, Pengkai, Xin, Jian, Liu, Tong, Xing, Weiman, Kim, Chanhong. The Arabidopsis CRUMPLED LEAF protein, a homolog of the cyanobacterial bilin lyase, retains the bilin-binding pocket for a yet unknown function. PLANT JOURNAL[J]. 2020, 104(4): 964-978, http://dx.doi.org/10.1111/tpj.14974.
[16] Keun Pyo Lee, Kaiwei Liu, Eun Yu Kim, Laura MedinaPuche, Haihong Dong, Jianli Duan, Mengping Li, Vivek Dogra, Yingrui Li, Ruiqing Lv, Zihao Li, Rosa LozanoDuran, Chanhong Kim. PLANT NATRIURETIC PEPTIDE A and Its Putative Receptor PNP-R2 Antagonize Salicylic Acid–Mediated Signaling and Cell Death. THE PLANT CELL. 2020, 32(7): 2237-2250, https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7346577/.
[17] Dogra, Vivek, Kim, Chanhong. Singlet Oxygen Metabolism: From Genesis to Signaling. FRONTIERS IN PLANT SCIENCE[J]. 2020, 10: https://doaj.org/article/da50d35da26d49a8802c7c57f05ad3c6.
[18] CHAN HONG KIM. N-terminal acetylation stabilizes SIGMA FACTOR BINDING PROTEIN 1 involved in salicylic acid-primed cell death.. Plant Physiology. 2020, [19] Duan, Jianli, Lee, Keun Pyo, Dogra, Vivek, Zhang, Siyuan, Liu, Kaiwei, CaceresMoreno, Carlos, Lv, Shanshan, Xing, Weiman, Kato, Yusuke, Sakamoto, Wataru, Liu, Renyi, Macho, Alberto P, Kim, Chanhong. Impaired PSII Proteostasis Promotes Retrograde Signaling via Salicylic Acid. PLANT PHYSIOLOGY[J]. 2019, 180(4): 2182-2197, https://www.webofscience.com/wos/woscc/full-record/WOS:000477951400035.
[20] Lv, Ruiqing, Li, Zihao, Li, Mengping, Dogra, Vivek, Lv, Shanshan, Liu, Renyi, Lee, Keun Pyo, Kim, Chanhong. Uncoupled Expression of Nuclear and Plastid Photosynthesis-Associated Genes Contributes to Cell Death in a Lesion Mimic Mutant. PLANT CELL[J]. 2019, 31(1): 210-230, https://www.webofscience.com/wos/woscc/full-record/WOS:000459774700020.
[21] CHAN HONG KIM. Impaired PSII proteostasis triggers an UPR-like response in the var2 mutant of Arabidopsis thaliana.. Journal of Experimental Botany. 2019, [22] Vivek Dogra, Mingyue Li, Somesh Singh, Mengping Li, Chanhong Kim. Oxidative post-translational modification of EXECUTER1 is required for singlet oxygen sensing in plastids. NATURE COMMUNICATIONS[J]. 2019, 10(1): 1-12, http://dx.doi.org/10.1038/s41467-019-10760-6.
[23] Pesaresi, Paolo, Kim, Chanhong. Current understanding of GUN1: a key mediator involved in biogenic retrograde signaling. PLANT CELL REPORTSnull. 2019, 38(7): 819-823, [24] Dogra, Vivek, Kim, Chanhong. Chloroplast protein homeostasis is coupled with retrograde signaling. PLANT SIGNALING & BEHAVIORnull. 2019, 14(11): https://www.webofscience.com/wos/woscc/full-record/WOS:000482846800001.
[25] ShangGuan, Keke, Wang, Min, Htwe, Nang Myint Phyu Sin, Li, Ping, Li, Yaoshen, Qi, Fan, Zhang, Dawei, Cao, Min, Kim, Chanhong, Weng, Haiyong, Cen, Haiyan, Black, Ian M, Azadi, Parastoo, Carlson, Russell W, Stacey, Gary, Liang, Yan. Lipopolysaccharides Trigger Two Successive Bursts of Reactive Oxygen Species at Distinct Cellular Locations. PLANT PHYSIOLOGY[J]. 2018, 176(3): 2543-2556, https://www.webofscience.com/wos/woscc/full-record/WOS:000426848300049.
[26] Rochaix, JeanDavid, Kim, Chanhong, Apel, Wiebke. Klaus Apel (1942-2017): a pioneer of photosynthesis research. PHOTOSYNTHESIS RESEARCHnull. 2018, 137(2): 153-159, https://www.webofscience.com/wos/woscc/full-record/WOS:000437397700001.
[27] CHAN HONG KIM. Singlet oxygen‐triggered chloroplast‐to‐nucleus retrograde signaling pathways: an emerging perspective. Plant, cell & environment. 2018, [28] Dogra, Vivek, Duan, Jianli, Lee, Keun Pyo, Lv, Shanshan, Liu, Renyi, Kim, Chanhong. FtsH2-Dependent Proteolysis of EXECUTER1 Is Essential in Mediating Singlet Oxygen-Triggered Retrograde Signaling in Arabidopsis thaliana. FRONTIERS IN PLANT SCIENCE[J]. 2017, 8: https://doaj.org/article/b2501b7b306b4b11a56b2d59fcddf5cc.
[29] Wang, Liangsheng, Kim, Chanhong, Xu, Xia, Piskurewicz, Urszula, Dogra, Vivek, Singh, Somesh, Mahler, Hanno, Apel, Klaus. Singlet oxygen- and EXECUTER1-mediated signaling is initiated in grana margins and depends on the protease FtsH2. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA[J]. 2016, 113(26): E3792-E3800, http://dx.doi.org/10.1073/pnas.1603562113.
[30] Chen, Shiguo, Kim, Chanhong, Lee, Je Min, Lee, HyunAh, Fei, Zhangjun, Wang, Liangsheng, Apel, Klaus. Blocking the Q(B)-binding site of photosystem II by tenuazonic acid, a non-host-specific toxin of Alternaria alternata, activates singlet oxygen-mediated and EXECUTER-dependent signalling in Arabidopsis. PLANT CELL AND ENVIRONMENT[J]. 2015, 38(6): 1069-1080, http://dx.doi.org/10.1111/pce.12462.
[31] Zhang, Shengrui, Apel, Klaus, Kim, Chanhong. Singlet oxygen-mediated and EXECUTER-dependent signalling and acclimation of Arabidopsis thaliana exposed to light stress. PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCESnull. 2014, 369(1640): http://dx.doi.org/10.1098/rstb.2013.0227.
[32] Kim, Chanhong, Apel, Klaus. Singlet oxygen-mediated signaling in plants: moving from flu to wild type reveals an increasing complexity. PHOTOSYNTHESIS RESEARCHnull. 2013, 116(2-3): 455-464, http://dx.doi.org/10.1007/s11120-013-9876-4.
[33] Chanhong Kim Klaus Apel. 1O2-Mediated and EXECUTER-Dependent Retrograde Plastid-to-Nucleus Signaling in Norflurazon-Treated Seedlings of Arabidopsis thaliana. 分子植物：英文版[J]. 2013, 1580-1591, http://lib.cqvip.com/Qikan/Article/Detail?id=47523360.
[34] Kim, Chanhong, Apel, Klaus. Arabidopsis light-dependent NADPH: protochlorophyllide oxidoreductase A (PORA) is essential for normal plant growth and development: an addendum. PLANT MOLECULAR BIOLOGY[J]. 2012, 80(2): 237-240, https://www.webofscience.com/wos/woscc/full-record/WOS:000307964400008.
[35] Kim, Chanhong, Meskauskiene, Rasa, Zhang, Shengrui, Lee, Keun Pyo, Ashok, Munusamy Lakshmanan, Blajecka, Karolina, Herrfurth, Cornelia, Feussner, Ivo, Apel, Klaus. Chloroplasts of Arabidopsis Are the Source and a Primary Target of a Plant-Specific Programmed Cell Death Signaling Pathway. PLANT CELL[J]. 2012, 24(7): 3026-3039, https://www.webofscience.com/wos/woscc/full-record/WOS:000308352800025.
[36] Klra imkov, Chanhong Kim, Katarzyna Gacek, Aiswarya Baruah, Christophe Laloi, Klaus Apel. The chloroplast division mutant caa33 of Arabidopsis thaliana reveals a crucial impact of chloroplast homeostasis on stress acclimation and retrograde plastid-to-nucleus signaling. THE PLANT JOURNAL. 2011, 69(4): 701-712, https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3274639/.
[37] Chanhong Kim, Keun Pyo Lee, Aiswarya Baruah, Mena Nater, Cornelia Gbel, Ivo Feussner, Klaus Apel. 1 O 2 -mediated retrograde signaling during late embryogenesis predetermines plastid differentiation in seedlings by recruiting abscisic acid. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 2009, 106(24): 9920-9924, [38] CHAN HONG KIM. No single way to understand singlet oxygen signaling in plants. EMBO reports. 2008, [39] Lee, Keun Pyo, Kim, Chanhong, Landgraf, Frank, Apel, Klaus. EXECUTER1- and EXECUTER2-dependent transfer of stress-related signals from the plastid to the nucleus of Arabidopsis thaliana. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA[J]. 2007, 104(24): 10270-10275, https://www.webofscience.com/wos/woscc/full-record/WOS:000247363000063.
[40] CHAN HONG KIM. Multiplicity of different cell and organs- specific import routes for the NADPH protochlorophyllide oxidoreductase A and B in plastids of Arabidopsis seedlings. Plant Journal. 2005, [41] Huq, E, AlSady, B, Hudson, M, Kim, CH, Apel, M, Quail, PH. PHYTOCHROME-INTERACTING FACTOR 1 is a critical bHLH regulator of chlorophyll biosynthesis. SCIENCE[J]. 2004, 305(5692): 1937-1941, https://www.webofscience.com/wos/woscc/full-record/WOS:000224136000040.
[42] CHAN HONG KIM. Substrate-dependent and organ specific chloroplast protein import in plants. Plant Cell. 2004, [43] CHAN HONG KIM. Concurret interactions of heme and FLU with Glu tRNA reductase, the target of metabolic feedback inhibition of tetrapyrrole biosynthesis in higher plants. Plant Journal. 2004, [44] Wagner, D, Przybyla, D, Camp, ROD, Kim, C, Landgraf, F, Lee, KP, Wursch, M, Laloi, C, Nater, M, Hideg, E, Apel, K. The genetic basis of singlet oxygen-induced stress responses of Arabidopsis thaliana. SCIENCE[J]. 2004, 306(5699): 1183-1185, https://www.webofscience.com/wos/woscc/full-record/WOS:000225193100049.
[45] Jang, SH, Lee, B, Kim, C, Kim, SJ, Yim, J, Han, JJ, Lee, S, Kim, SR, An, G. The OsFOR1 gene encodes a polygalacturonase-inhibiting protein (PGIP) that regulates floral organ number in rice. PLANT MOLECULAR BIOLOGY[J]. 2003, 53(3): 357-369, https://www.webofscience.com/wos/woscc/full-record/WOS:000186986400009.
[46] Lee, KP, Kim, C, Lee, DW, Apel, K. TIGRINA d, required for regulating the biosynthesis of tetrapyrroles in barley, is an ortholog of the FLU gene of Arabidopsis thaliana. FEBS LETTERS[J]. 2003, 553(1-2): 119-124, http://dx.doi.org/10.1016/S0014-5793(03)00983-9.
[47] CHAN HONG KIM. Effect of cultural conditions on the biosynthesis of gagaminine, a potent antioxidant from the roots of Cynanchumwilfordii. Biological & Pharmaceutical Bulletin. 2001, [48] Kim, CH, Jeong, DH, An, GH. Molecular cloning and characterization of OsLRK1 encoding a putative receptor-like protein kinase from Oryza sativa. PLANT SCIENCE[J]. 2000, 152(1): 17-26, http://dx.doi.org/10.1016/S0168-9452(99)00216-2.

   

（ 1 ） 解析在应对环境胁迫时叶绿体作为感应器和触发器的新兴角色, 主持, 国家级, 2016-01--2019-12

（1）2   第五届生物植物相互作用国际会议（ICBPI）   2017-08-17
（2）1   2017泰山植物逆境生物学国际论坛   2017-06-02
（3）3   第十届国际植物线粒体生物学大会2017（ICPMB2017）   2017-05-22
（4）Battles in Chloroplasts: to live or to die   2015年第26届拟南芥研究国际大会   2015-07-05

现指导学生

李明月  博士研究生  071010-生物化学与分子生物学  

吕瑞清  博士研究生  071010-生物化学与分子生物学  

李子豪  博士研究生  071010-生物化学与分子生物学  

方君  博士研究生  071010-生物化学与分子生物学  

刘开玮  硕士研究生  071010-生物化学与分子生物学  

李梦萍  博士研究生  071010-生物化学与分子生物学  

张思远  博士研究生  071010-生物化学与分子生物学  

李炳圻  博士研究生  071010-生物化学与分子生物学  

崔超军  硕士研究生  071007-遗传学