1. 生物催化与蛋白质工程：a）生物信息学挖掘新型工业用酶；b）生物催化剂的分子定向进化改造；c）计算机(In Silico)定向进化与设计；d) 新酶新反应设计与催化机制研究。

2. 工业微生物代谢工程与合成生物学。

   

071005-微生物学
071010-生物化学与分子生物学

生物催化与定向进化
酶分子工程
代谢工程

2005-09--2012-03   中国科学院上海生命科学研究院   博士研究生
2003-09--2005-06   河南大学   工学学士

研究生

博士

2016-9～至今，中国科学院天津工业生物技术研究所，研究员

2013-10~2016-09,德国马普煤炭化学研究所, 博士后

2012-10~2013-09,新加坡南洋理工大学, 博士后研究员

2022-12-01-今,生物工程学报, 编委
2021-11-01-2026-11-01,中国生物发酵产业协会酶制剂分会, 理事
2021-10-01-今,中国微生物学会酶工程专业委员会, 委员
2021-06-29-2026-06-28,天津市化工学会, 常务理事
2020-11-14-2025-11-13,天津市青年科技工作者协会, 监事长
2020-07-02-今,生物学杂志, 副主任委员
2020-01-01-今,Green Synthesis & Catalysis, 编委
2020-01-01-今,Bioresources and Bioprocessing, 编委
2019-09-01-今,中国科学技术大学博导, 博士生导师
2019-08-10-今,中国生物化学与分子生物学会酶学专业分会, 委员
2019-03-15-今,南开大学生命科学学院, 硕士研究生校外指导老师

   

（1） 二氧化碳到淀粉的人工合成, 特等奖, 省级, 2022
（2） 中国微生物学会优秀青年酶工程学家奖, 其他, 2021
（3） 中国产学研合作创新成果奖, , 其他, 2014

[1] 刘涛, 毕慧萍, 孙周通, 曲戈, 王帅, 庄以彬, 马延和. 糖链延伸糖基转移酶突变体及其编码基因以及基因工程菌和它们的应用. CN: CN114032222A, 2022-02-11.
[2] 张武元, 张鹏鹏, 孙周通. 一种过氧化酶催化氧化芳香烃及其衍生物的方法. CN: CN113999879A, 2022-02-01.
[3] 张武元, 陈少航, 孙周通, 马延和. 一种由过氧化物酶催化制备β-卤代醚和β-卤代醇的方法. CN: CN113549659A, 2021-10-26.
[4] 张武元, 张鹏鹏, 孙周通, 马延和. 一种合成多官能团苯胺类化合物的光促酶催化方法. CN: CN113528594A, 2021-10-22.
[5] 孙周通, 王红月, 曲戈. 一种双酶级联催化合成(R)-3-氨基-1-丁醇的方法. CN: CN112852895A, 2021-05-28.
[6] 孙周通, 曲戈, 刘贝贝, 毕悦欣. 醇脱氢酶突变体及其在高效不对称还原制备手性双芳基醇化合物中的应用. CN: CN112852770A, 2021-05-28.
[7] 孙周通, 王红月, 曲戈, 蒋迎迎. 胺脱氢酶突变体及其在手性胺醇化合物合成中的应用. CN: CN112852894A, 2021-05-28.
[8] 孙周通, 杨大猛, 曲戈, 蒋迎迎, 李超, 刘保艳. 脱乙酰氧头孢菌素C合成酶突变体及其在β-内酰胺抗生素母核合成中的应用. CN: CN112852913A, 2021-05-28.
[9] 孙周通, 杨大猛. 一种合成7-氨基去乙酰氧基头孢烷酸的方法. CN: CN112852912A, 2021-05-28.
[10] 孙周通, 杨大猛. 一种头孢菌素C生产菌及其制备方法与应用. CN: CN112852907A, 2021-05-28.
[11] 孙周通, 王红月, 刘贝贝. 一种手性胺醇化合物的合成方法. CN: CN112779232A, 2021-05-11.
[12] 孙周通, 张锟, 梅泽龙, 涂然, 曲戈. 一种高通量筛选酮类化合物的检测方法及其在酶筛选中的应用. CN: CN112394050A, 2021-02-23.
[13] 孙周通, 刘贝贝, 曲戈, 刘保艳. 醇脱氢酶突变体及其在手性双芳基醇化合物合成中的应用. CN: CN111100851A, 2020-05-05.
[14] 孙周通, 赵强, 刘保艳, 刘贝贝, 闫豪杰. 一种手性3-氨基-1-丁醇的合成方法. CN: CN110551771A, 2019-12-10.
[15] 孙周通, 刘保艳, 刘贝贝, 曲戈. 一种手性双芳基醇化合物的合成方法. CN: CN109837317A, 2019-06-04.
[16] 孙周通, 赵强, 刘保艳, 曲戈. 一种手性2-氨基-1-丁醇的合成方法. CN: CN109825538A, 2019-05-31.
[17] 杨晟, 陶荣盛, 朱傅赟, 赵丽丽, 蒋宇, 杨俊杰, 孙周通, 沈正权, 黄鹤, 孙梁栋, 董枫, 刘映淼. 用于生产L-2-氨基丁酸的载体、工程菌株及方法. CN: CN103215291A, 2013-07-24.
[18] 杨晟, 黄鹤, 孙周通. 一种二氢吡啶二羧酸合成酶. CN: CN102191227A, 2011-09-21.

   

[1] Qiang Chen, Ge Qu, Xu Li, Mingjian Feng, Fan Yang, Yanjie Li, Jincheng Li, Feifei Tong, Shiyi Song, Yujun Wang, Zhoutong Sun, Guangsheng Luo. Active and stable alcohol dehydrogenase-assembled hydrogels via synergistic bridging of triazoles and metal ions. NATURE COMMUNICATIONS[J]. 2023, 14(1): 1-12, https://doaj.org/article/8a23f4f93e4849218588d536eb254723.
[2] 王钦宏, 张以恒, 田朝光, 孙周通, 马延和. 低碳生物合成：机遇与挑战. 科学通报[J]. 2023, [3] Li, Yinuo, Bao, Cuiyu, Sun, Zhoutong, Zhang, Wuyuan, Yuan, Bo. Comparison between Chemoenzymatic and Bienzymatic Cascades Leading to Morita-Baylis-Hillman Adducts. CHEMCATCHEM. 2023, http://dx.doi.org/10.1002/cctc.202201621.
[4] Jun-Kuan Li, Ge Qu, Xu Li, Yuchen Tian, Chengsen Cui, Fa-Guang Zhang, Wuyuan Zhang, Jun-An Ma, Manfred T. Reetz, Zhoutong Sun. Rational enzyme design for enabling biocatalytic Baldwin cyclization and asymmetric synthesis of chiral heterocycles. NATURE COMMUNICATIONS[J]. 2022, 13(1): http://dx.doi.org/10.1038/s41467-022-35468-y.
[5] Zhang, Jun, Liao, Daohong, Chen, Rongchang, Zhu, Fangfang, Ma, Yaqing, Gao, Lei, Qu, Ge, Cui, Chengsen, Sun, Zhoutong, Lei, Xiaoguang, Gao, ShuShan. Tuning an Imine Reductase for the Asymmetric Synthesis of Azacycloalkylamines by Concise Structure-Guided Engineering. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION[J]. 2022, 61(24): http://dx.doi.org/10.1002/anie.202201908.
[6] Wang, Peipei, Han, Xiaofeng, Liu, Xinqi, Lin, Richen, Chen, Yongzheng, Sun, Zhoutong, Zhang, Wuyuan. Synthesis of Enantioenriched Sulfoxides by an Oxidation-Reduction Enzymatic Cascade. CHEMISTRY-A EUROPEAN JOURNAL[J]. 2022, 28(61): http://dx.doi.org/10.1002/chem.202201997.
[7] Ge, Ran, Zhang, Pengpeng, Dong, Xuetian, Li, Yuanying, Sun, Zhoutong, Zeng, Yongyi, Chen, Bishuang, Zhang, Wuyuan. Photobiocatalytic Decarboxylation for the Synthesis of Fatty Epoxides from Renewable Fatty Acids. CHEMSUSCHEM[J]. 2022, 15(20): http://dx.doi.org/10.1002/cssc.202201275.
[8] Yingying Jiang, Ge Qu, Xiang Sheng, Feifei Tong, Zhoutong Sun. Unraveling the mechanism of enantio-controlling switches of an alcohol dehydrogenase toward sterically small ketone. Catalysis Science & Technology[J]. 2022, 12(6): 1777-1787, https://pubs.rsc.org/en/content/articlelanding/2022/cy/d2cy00031h/unauth.
[9] Jiang, Yingying, Li, Xu, Liu, Beibei, Tong, Feifei, Qu, Ge, Sun, Zhoutong. Engineering the hydrogen transfer pathway of an alcohol dehydrogenase to increase activity by rational enzyme design. MOLECULAR CATALYSIS[J]. 2022, 530: http://dx.doi.org/10.1016/j.mcat.2022.112603.
[10] Chen, Qiang, An, Yuchao, Feng, Mingjian, Li, Jincheng, Li, Yanjie, Tong, Feifei, Qu, Ge, Sun, Zhoutong, Wang, Yujun, Luo, Guangsheng. An enzyme-assembled gel monolithic microreactor for continuous flow asymmetric synthesis of aryl alcohols. GREEN CHEMISTRY[J]. 2022, 24(24): 9508-9518, http://dx.doi.org/10.1039/d2gc03082a.
[11] 毕悦欣, 蒋迎迎, 覃宗敏, 曲戈, 孙周通. 醇脱氢酶金属离子绑定位点的可替换性. 生物工程学报[J]. 2022, 38(4): 1518-1526, http://lib.cqvip.com/Qikan/Article/Detail?id=7107164588.
[12] Bi, Huiping, Qu, Ge, Wang, Shuai, Zhuang, Yibin, Sun, Zhoutong, Liu, Tao, Ma, Yanhe. Biosynthesis of a rosavin natural product in Escherichia coli by glycosyltransferase rational design and artificial pathway construction. METABOLIC ENGINEERING[J]. 2022, 69: 15-25, [13] Dong, Chen, Qu, Ge, Guo, Jinggong, Wei, Fang, Gao, Shuwen, Sun, Zhoutong, Jin, Lifeng, Sun, Xuwu, Rochaix, JeanDavid, Miao, Yuchen, Wang, Ran. Rational design of geranylgeranyl diphosphate synthase enhances carotenoid production and improves photosynthetic efficiency in Nicotiana tabacum. SCIENCE BULLETIN[J]. 2022, 67(3): 315-327, https://www.sciengine.com/doi/10.1016/j.scib.2021.07.003.
[14] Huang, Wansheng, Huang, Shengtang, Sun, Zhoutong, Zhang, Wuyuan, Zeng, Zhigang, Yuan, Bo. Chemoenzymatic Synthesis of Sterically Hindered Biaryls by Suzuki Coupling and Vanadium Chloroperoxidase Catalyzed Halogenations. CHEMBIOCHEM. 2022, http://dx.doi.org/10.1002/cbic.202200610.
[15] Li, Yuanying, Zhang, Pengpeng, Sun, Zhoutong, Li, Huanhuan, Ge, Ran, Sheng, Xiang, Zhang, Wuyuan. Peroxygenase-Catalyzed Selective Synthesis of Calcitriol Starting from Alfacalcidol. ANTIOXIDANTS[J]. 2022, 11(6): http://apps.webofknowledge.com/CitedFullRecord.do?product=UA&colName=WOS&SID=5CCFccWmJJRAuMzNPjj&search_mode=CitedFullRecord&isickref=WOS:000817441100001.
[16] Wu, Xiaowen, Jiang, Yingying, Wang, Zengyu, Yu, Xiaobin, Sun, Zhoutong, Luo, Wei. Enhanced thermostability of formate dehydrogenase via semi-rational design. MOLECULAR CATALYSIS[J]. 2022, 530: http://dx.doi.org/10.1016/j.mcat.2022.112628.
[17] Ming, Hui, Yuan, Bo, Qu, Ge, Sun, Zhoutong. Engineering the activity of amine dehydrogenase in the asymmetric reductive amination of hydroxyl ketones. CATALYSIS SCIENCE & TECHNOLOGY[J]. 2022, 12(19): 5952-5960, http://dx.doi.org/10.1039/d2cy00391k.
[18] Sang, Xianke, Tong, Feifei, Zeng, Zhigang, Wu, Minghu, Yuan, Bo, Sun, Zhoutong, Sheng, Xiang, Qu, Ge, Alcalde, Miguel, Hollmann, Frank, Zhang, Wuyuan. A Biocatalytic Platform for the Synthesis of Enantiopure Propargylic Alcohols and Amines. ORGANIC LETTERS[J]. 2022, 24(23): 4252-4257, http://dx.doi.org/10.1021/acs.orglett.2c01547.
[19] Shiyi Song, Yingying Jiang, Ruidong Chen, Wencheng Su, Weinan Liang, Dameng Yang, Jincheng Li, Wuyuan Zhang, Shushan Gao, Bo Yuan, Ge Qu, Zhoutong Sun. Whole-cell Biotransformation of Penicillin G by a Three-enzyme Co-expression System with Engineered Deacetoxycephalosporin C Synthase. ChemBioChem[J]. 2022, e202200179-, https://chemistry-europe.onlinelibrary.wiley.com/doi/10.1002/cbic.202200179.
[20] Ge Qu, Yuexin Bi, Beibei Liu, Junkuan Li, Xu Han, Weidong Liu, Yingying Jiang, Zongmin Qin, Zhoutong Sun. Unlocking the stereoselectivity and substrate acceptance of enzymes: Proline induced loop engineering test. Angewandte Chemie International Edition[J]. 2022, 61(1): e202110793-, https://onlinelibrary.wiley.com/doi/10.1002/anie.202110793.
[21] 曲戈, 袁波, 孙周通. 工业蛋白质理性设计与应用. 生物工程学报[J]. 2022, 38(11): 4068-4080, http://lib.cqvip.com/Qikan/Article/Detail?id=7108545584.
[22] Yu Zhipu, An Chunyan, Yao Yongpeng, Wang Changyun, Sun Zhoutong, Cui Chengsen, Liu Ling, Gao Shushan. A combined strategy for the overproduction of complex ergot alkaloid agroclavine. SYNTHETIC AND SYSTEMS BIOTECHNOLOGY[J]. 2022, 7(4): 1126-1132, http://sciencechina.cn/gw.jsp?action=detail.jsp&internal_id=7414703&detailType=1.
[23] Dameng Yang, Wencheng Su, Yingying Jiang, Shushan Gao, Xiangying Li, Ge Qu, Zhoutong Sun. Biosynthesis of β-lactam nuclei in yeast. Metabolic Engineering[J]. 2022, 72: 56-65, https://www.sciencedirect.com/science/article/pii/S1096717622000350?via%3Dihub.
[24] Feifei Tong, Zongmin Qin, Hongyue Wang, Yingying Jiang, Junkuan Li, Hui Ming, Ge Qu, Yazhong Xiao, Zhoutong Sun. Biosynthesis of Chiral Amino Alcohols via an Engineered Amine Dehydrogenase in E. coli. FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY[J]. 2022, 9: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8766677/.
[25] Wang, Juan, Qu, Ge, Xie, Leipeng, Gao, Chao, Jiang, Yingying, Zhang, YiHeng P Job, Sun, Zhoutong, You, Chun. Engineering of a thermophilic dihydroxy-acid dehydratase toward glycerate dehydration for in vitro biosystems. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY[J]. 2022, 106(9-10): 3625-3637, http://dx.doi.org/10.1007/s00253-022-11936-9.
[26] Zongmin Qin, Xiaohui Zhang, Xianke Sang, Wuyuan Zhang, Ge Qu, Zhoutong Sun. Carboxylic acid reductases enable intramolecular lactamization reactions. GREEN SYNTHESIS AND CATALYSIS[J]. 2022, 3(3): 294-297, http://dx.doi.org/10.1016/j.gresc.2022.05.009.
[27] Chen, Qiang, Guo, Mingzhao, Bi, Yuexin, Qu, Ge, Sun, Zhoutong, Wang, Yujun, Luo, Guangsheng. Whole-cell biocatalytic synthesis of S-(4-chlorophenyl)-(pyridin-2-yl) methanol in a liquid?liquid biphasic microreaction system. BIORESOURCE TECHNOLOGY[J]. 2021, 330: http://dx.doi.org/10.1016/j.biortech.2021.125022.
[28] Jiao Li, Ge Qu, Na Shang, Peng Chen, Yan Men, Weidong Liu, Zelong Mei, Yuanxia Sun, Zhoutong Sun. Near-perfect control of the regioselective glucosylation enabled by rational design of glycosyltransferases. GREEN SYNTHESIS AND CATALYSIS[J]. 2021, 2(1): 45-53, http://dx.doi.org/10.1016/j.gresc.2021.01.005.
[29] AcevedoRocha, Carlos G, Hollmann, Frank, Sanchis, Joaquin, Sun, Zhoutong. A Pioneering Career in Catalysis: Manfred T. Reetz. ACS CATALYSIS[J]. 2020, 10(24): 15123-15139, [30] Zelong Mei, Kun Zhang, Ge Qu, JunKuan Li, Baoyan Liu, JunAn Ma, Ran Tu, Zhoutong Sun. High-Throughput Fluorescence Assay for Ketone Detection and Its Applications in Enzyme Mining and Protein Engineering. ACS OMEGA[J]. 2020, 5(23): 13588-13594, https://www.webofscience.com/wos/woscc/full-record/WOS:000542223300013.
[31] Chen, Jing, Fan, Feiyu, Qu, Ge, Tang, Jinlei, Xi, Yongyan, Bi, Changhao, Sun, Zhoutong, Zhang, Xueli. Identification of Absidia orchidis steroid 11 beta-hydroxylation system and its application in engineering Saccharomyces cerevisiae for one-step biotransformation to produce hydrocortisone. METABOLIC ENGINEERING[J]. 2020, 57: 31-42, https://www.webofscience.com/wos/woscc/full-record/WOS:000506206200004.
[32] Jing Chen, Feiyu Fan, Ge Qu, Jinlei Tang, Yongyan Xi, Changhao Bi, Zhoutong Sun, Xueli Zhang. Identification of Absidia orchidis steroid 11β-hydroxylation system and its application in engineering Saccharomyces cerevisiae for one-step biotransformation to produce hydrocortisone. METABOLIC ENGINEERING. 2020, 57: 31-42, http://dx.doi.org/10.1016/j.ymben.2019.10.006.
[33] Qu, Ge, Li, Aitao, AcevedoRocha, Carlos G, Sun, Zhoutong, Reetz, Manfred T. The Crucial Role of Methodology Development in Directed Evolution of Selective Enzymes. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION[J]. 2020, 59(32): 13204-13231, https://www.webofscience.com/wos/woscc/full-record/WOS:000521629200001.
[34] Wang, Hongyue, Qu, Ge, Li, JunKuan, Ma, JunAn, Guo, Jinggong, Miao, Yuchen, Sun, Zhoutong. Data mining of amine dehydrogenases for the synthesis of enantiopure amino alcohols. CATALYSIS SCIENCE & TECHNOLOGY[J]. 2020, 10(17): 5945-5952, https://www.webofscience.com/wos/woscc/full-record/WOS:000565991300018.
[35] 蒋迎迎, 曲戈, 孙周通. 机器学习助力酶定向进化. 生物学杂志[J]. 2020, 37(4): 1-11, https://kns.cnki.net/KCMS/detail/detail.aspx?dbcode=CJFQ&dbname=CJFDLAST2020&filename=SWXZ202004001&v=MjE1Mjl1ZHVGeW5tVTczS05qclRkTEc0SE5ITXE0OUZaWVI4ZVgxTHV4WVM3RGgxVDNxVHJXTTFGckNVUjdxZVo=.
[36] Dai, Zhubo, Liu, Yun, Sun, Zhoutong, Wang, Dong, Qu, Ge, Ma, Xiaolin, Fan, Feiyu, Zhang, Lili, Li, Shoulian, Zhang, Xueli. Identification of a novel cytochrome P450 enzyme that catalyzes the C-2 alpha hydroxylation of pentacyclic triterpenoids and its application in yeast cell factories. METABOLIC ENGINEERING[J]. 2019, 51: 70-78, https://www.webofscience.com/wos/woscc/full-record/WOS:000456420200008.
[37] Li, Aitao, Qu, Ge, Sun, Zhoutong, Reetz, Manfred T. Statistical Analysis of the Benefits of Focused Saturation Mutagenesis in Directed Evolution Based on Reduced Amino Acid Alphabets. ACS CATALYSIS[J]. 2019, 9(9): 7769-7778, https://www.webofscience.com/wos/woscc/full-record/WOS:000485090400015.
[38] Qu, Ge, Liu, Beibei, Jiang, Yingying, Nie, Yao, Yu, Huilei, Sun, Zhoutong. Laboratory evolution of an alcohol dehydrogenase towards enantioselective reduction of difficult-to-reduce ketones. BIORESOURCES AND BIOPROCESSING[J]. 2019, 6(1): http://dx.doi.org/10.1186/s40643-019-0253-9.
[39] 曲戈, 朱彤, 蒋迎迎, 吴边, 孙周通. 蛋白质工程：从定向进化到计算设计. 生物工程学报[J]. 2019, 35(10): 1843-1856, http://lib.cqvip.com/Qikan/Article/Detail?id=7100162087.
[40] 曲戈, 张锟, 蒋迎迎, 孙周通. 2018诺贝尔化学奖：酶定向进化与噬菌体展示技术. 生物学杂志[J]. 2019, 36(1): 1-6, http://lib.cqvip.com/Qikan/Article/Detail?id=7001409142.
[41] 张锟, 曲戈, 刘卫东, 孙周通. 工业酶结构与功能的构效关系. 生物工程学报[J]. 2019, 35(10): 1806-1818, http://lib.cqvip.com/Qikan/Article/Detail?id=7100162084.
[42] Sun, Zhoutong, Liu, Qian, Qu, Ge, Feng, Yan, Reetz, Manfred T. Utility of B-Factors in Protein Science: Interpreting Rigidity, Flexibility, and Internal Motion and Engineering Thermostability. CHEMICAL REVIEWSnull. 2019, 119(3): 1626-1665, http://dx.doi.org/10.1021/acs.chemrev.8b00290.
[43] Liu, Beibei, Qu, Ge, Li, JunKuan, Fan, Wenchao, Ma, JunAn, Xu, Yan, Nie, Yao, Sun, Zhoutong. Conformational Dynamics-Guided Loop Engineering of an Alcohol Dehydrogenase: Capture, Turnover and Enantioselective Transformation of Difficult-to-Reduce Ketones. ADVANCED SYNTHESIS & CATALYSIS[J]. 2019, 361(13): 3182-3190, https://www.webofscience.com/wos/woscc/full-record/WOS:000484142600018.
[44] Zhubo Dai, Yun Liu, Zhoutong Sun, Dong Wang, Ge Qu, Xiaolin Ma, Feiyu Fan, Lili Zhang, Shoulian Li, Xueli Zhang. Identification of a novel cytochrome P450 enzyme that catalyzes the C-2α hydroxylation of pentacyclic triterpenoids and its application in yeast cell factories. METABOLIC ENGINEERING. 2019, 51: 70-78, http://dx.doi.org/10.1016/j.ymben.2018.10.001.
[45] Qu, Ge, Fu, Mingxing, Zhao, Lili, Liu, Beibei, Liu, Pi, Fan, Wenchao, Ma, JunAn, Sun, Zhoutong. Computational Insights into the Catalytic Mechanism of Bacterial Carboxylic Acid Reductase. JOURNAL OF CHEMICAL INFORMATION AND MODELING[J]. 2019, 59(2): 832-841, [46] Qu, Ge, Liu, Beibei, Zhang, Kun, Jiang, Yingying, Guo, Jinggong, Wang, Ran, Miao, Yuchen, Zhai, Chao, Sun, Zhoutong. Computer-assisted engineering of the catalytic activity of a carboxylic acid reductase. JOURNAL OF BIOTECHNOLOGY[J]. 2019, 306: 97-104, http://dx.doi.org/10.1016/j.jbiotec.2019.09.006.
[47] Li, Aitao, AcevedoRocha, Carlos G, Sun, Zhoutong, Cox, Tony, Xu, Jia Lucy, Reetz, Manfred T. Beating Bias in the Directed Evolution of Proteins: Combining High-Fidelity on-Chip Solid-Phase Gene Synthesis with Efficient Gene Assembly for Combinatorial Library Construction. CHEMBIOCHEM[J]. 2018, 19(3): 221-228, https://www.webofscience.com/wos/woscc/full-record/WOS:000424105700006.
[48] Qu, Ge, Guo, Jinggong, Yang, Dameng, Sun, Zhoutong. Biocatalysis of carboxylic acid reductases: phylogenesis, catalytic mechanism and potential applications. GREEN CHEMISTRY[J]. 2018, 20(4): 777-792, https://www.webofscience.com/wos/woscc/full-record/WOS:000425466900002.
[49] 曲戈, 赵晶, 郑平, 孙际宾, 孙周通. 定向进化技术的最新进展. 生物工程学报[J]. 2018, 34(1): 1-11, http://lib.cqvip.com/Qikan/Article/Detail?id=674348257.
[50] Qu, Ge, Lonsdale, Richard, Yao, Peiyuan, Li, Guangyue, Liu, Beibei, Reetz, Manfred T, Sun, Zhoutong. Methodology Development in Directed Evolution: Exploring Options when Applying Triple-Code Saturation Mutagenesis. CHEMBIOCHEM[J]. 2018, 19(3): 239-246, https://www.webofscience.com/wos/woscc/full-record/WOS:000424105700008.
[51] Yang, Jiangang, Zhu, Yueming, Qu, Ge, Zeng, Yan, Tian, Chaoyu, Dong, Caixia, Men, Yan, Dai, Longhai, Sun, Zhoutong, Sun, Yuanxia, Ma, Yanhe. Biosynthesis of dendroketose from different carbon sources using in vitro and in vivo metabolic engineering strategies. BIOTECHNOLOGY FOR BIOFUELS[J]. 2018, 11(1): https://doaj.org/article/96f147a8ff334e1fb1770283fe511793.
[52] AcevedoRocha, Carlos G, Sun, Zhoutong, Reetz, Manfred T. Clarifying the Difference between Iterative Saturation Mutagenesis as a Rational Guide in Directed Evolution and OmniChange as a Gene Mutagenesis Technique. CHEMBIOCHEMnull. 2018, 19(24): 2542-2544, https://www.webofscience.com/wos/woscc/full-record/WOS:000453835200003.
[53] Sun, Zhoutong, Wu, Lian, Bocola, Marco, Chan, H C Stephen, Lonsdale, Richard, Kong, XuDong, Yuan, Shuguang, Zhou, Jiahai, Reetz, Manfred T. Structural and Computational Insight into the Catalytic Mechanism of Limonene Epoxide Hydrolase Mutants in Stereoselective Transformations. JOURNAL OF THE AMERICAN CHEMICAL SOCIETY[J]. 2018, 140(1): 310-318, http://dx.doi.org/10.1021/jacs.7b10278.
[54] Li, Aitao, Sun, Zhoutong, Reetz, Manfred T. Solid-Phase Gene Synthesis for Mutant Library Construction: The Future of Directed Evolution?. CHEMBIOCHEM[J]. 2018, 19(19): 2023-2032, https://www.webofscience.com/wos/woscc/full-record/WOS:000446429400002.
[55] Sun, Zhoutong, Li, Guangyue, Ilie, Adriana, Reetz, Manfred T. Exploring the substrate scope of mutants derived from the robust alcohol dehydrogenase TbSADH. TETRAHEDRON LETTERS[J]. 2016, 57(32): 3648-3651, http://dx.doi.org/10.1016/j.tetlet.2016.06.134.
[56] Li, Aitao, Ilie, Adriana, Sun, Zhoutong, Lonsdale, Richard, Xu, JianHe, Reetz, Manfred T. Whole-Cell-Catalyzed Multiple Regio- and Stereoselective Functionalizations in Cascade Reactions Enabled by Directed Evolution. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION[J]. 2016, 55(39): 12026-12029, https://www.webofscience.com/wos/woscc/full-record/WOS:000384713100060.
[57] Sun, Zhoutong, Wikmark, Ylva, Backvall, JanE, Reetz, Manfred T. New Concepts for Increasing the Efficiency in Directed Evolution of Stereoselective Enzymes. CHEMISTRY-A EUROPEAN JOURNAL[J]. 2016, 22(15): 5046-5054, http://dx.doi.org/10.1002/chem.201504406.
[58] Sun, Zhoutong, Lonsdale, Richard, Ilie, Adriana, Li, Guangyue, Zhou, Jiahai, Reetz, Manfred T. Catalytic Asymmetric Reduction of Difficult-to-Reduce Ketones: Triple-Code Saturation Mutagenesis of an Alcohol Dehydrogenase. ACS CATALYSIS[J]. 2016, 6(3): 1598-1605, https://www.webofscience.com/wos/woscc/full-record/WOS:000371755500024.
[59] Sun, Zhoutong, Lonsdale, Richard, Li, Guangyue, Reetz, Manfred T. Comparing Different Strategies in Directed Evolution of Enzyme Stereoselectivity: Single- versus Double-Code Saturation Mutagenesis. CHEMBIOCHEM[J]. 2016, 17(19): 1865-1872, https://www.webofscience.com/wos/woscc/full-record/WOS:000385700900013.
[60] Li, Guangyue, Zhang, Hui, Sun, Zhoutong, Liu, Xinqi, Reetz, Manfred T. Multiparameter Optimization in Directed Evolution: Engineering Thermostability, Enantioselectivity, and Activity of an Epoxide Hydrolase. ACS CATALYSIS[J]. 2016, 6(6): 3679-3687, https://www.webofscience.com/wos/woscc/full-record/WOS:000377326700035.
[61] Sun, Zhoutong, Ilie, Adriana, Reetz, Manfred T. Towards the Production of Universal Blood by Structure-Guided Directed Evolution of Glycoside Hydrolases. ANGEWANDTE CHEMIE-INTERNATIONAL EDITIONnull. 2015, 54(32): 9158-9160, https://www.webofscience.com/wos/woscc/full-record/WOS:000358621500003.
[62] Sun, Zhoutong, Lonsdale, Richard, Kong, XuDong, Xu, JianHe, Zhou, Jiahai, Reetz, Manfred T. Reshaping an Enzyme Binding Pocket for Enhanced and Inverted Stereoselectivity: Use of Smallest Amino Acid Alphabets in Directed Evolution. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION[J]. 2015, 54(42): 12410-12415, https://www.webofscience.com/wos/woscc/full-record/WOS:000363397300028.
[63] Yang Chen, Jiang Weihong, Liu Yingmiao, Sun Bingbing, Liu Lixia, Ning Yuanyuan, Sun Zhoutong, Yang Sheng. Metabolic engineering of the L-phenylalanine pathway in Escherichia coli for the production of S- or R-mandelic acid. MICROBIAL CELL FACTORIES[J]. 2011, 10(1): https://doaj.org/article/8b4044127b314ae3b5a8a50290d2c218.
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（1） Controlling the Regio- and Stereoselectivity of Cytochrome P450 Monooxygenases by Protein Engineering. In Dioxygen-dependent Heme Enzymes, The Royal Society of Chemistry, 2018-10, 第 1 作者
（2） In silico prediction methods for site-saturation mutagenesis In “Enzyme Engineering: Methods and Protocols” of Methods in Molecular Biology, Humana Press, 2021-11, 第 2 作者
（3） 高级酶工程, Advanced Enzyme Engineering, 科学出版社, 2022-11, 第 2 作者
（4） Enzyme Engineering: Selective Catalysts for Applications in Biotechnology, Organic Chemistry, and Life Science, Wiley-VCH, 2023-02, 第 2 作者

   

（ 1 ） 中科院****C类, 主持, 部委级, 2016-09--2018-08
（ 2 ） 中科院****C类所匹配, 主持, 研究所（学校）, 2016-09--2018-08
（ 3 ） 功能酶库构建及筛选平台建设, 主持, 部委级, 2016-11--2018-06
（ 4 ） 醇脱氢酶TbSADH半理性设计影响合成重要医药中间体(S)-(4-氯苯基)吡啶-2-甲醇的研究, 主持, 国家级, 2018-01--2020-12
（ 5 ） S-2-氨基丁醇的生物合成, 主持, 部委级, 2017-05--2019-04
（ 6 ） 酶催化机制的计算解析, 主持, 部委级, 2018-01--2019-12
（ 7 ） S-2-氨基丁醇的酶法合成, 主持, 院级, 2018-01--2022-12
（ 8 ） 7-氨基头孢烷酸(7-ACA)的绿色生物法合成, 参与, 部委级, 2018-05--2019-04
（ 9 ） 亮氨酸脱氢酶催化合成（S）-2-氨基-1-丁醇的新反应设计与催化机制研究, 主持, 国家级, 2019-01--2022-12
（ 10 ） ****择优入选, 主持, 部委级, 2019-01--2021-12
（ 11 ） 人工生物酶的智能设计与塑造, 主持, 国家级, 2020-01--2024-12

（1）Enzyme directed evolution and rational design   第四届中日生物催化与转化学术论坛   2022-12-26
（2）The crucial role of rational enzyme evolution in innovating biocatalysis   第15届亚洲生物技术大会   2022-10-02
（3）酶分子设计与生物催化   第十三届中国酶工程学术研讨会   2021-12-04
（4）高效酶进化方法的开发与应用   第十一届全国化学生物学学术会议   2019-11-18
（5）先进定向进化方法开发与手性催化应用   第13届全国酶学学术讨论会   2017-10-18
（6）单密码子扫描重塑酶结合口袋：有效提高或反转立体选择性   第八届中国工业生物技术发展高峰论坛   孙周通   2015-12-11
（7）Reshaping an Enzyme Binding Pocket for Enhanced and Inverted Stereoselectivity: Use of Smallest Amino Acid Alphabets in Directed Evolution   19. Zhoutong Sun, Richard Lonsdale, Xu-Dong Kong, Jian-He Xu, Jiahai Zhou* and Manfred T. Reetz*   2015-07-26
（8）改造大肠杆菌芳香族氨基酸代谢途径并引入异源酶编码基因合成手性扁桃酸及D-苯甘氨酸   第五届全国发酵工程学术研讨会   孙周通、杨晟   2011-11-14
（9）Metabolic engineering of Escherichia coli L-phenylalanine pathway for the production of S- or R-mandelic acid   Zhoutong Sun, Weihong Jiang, Sheng Yang   2010-06-13

现指导学生

王红月  硕士研究生  071010-生物化学与分子生物学  

杨大猛  博士研究生  071010-生物化学与分子生物学  

蒋迎迎  博士研究生  071010-生物化学与分子生物学  

刘德川  硕士研究生  085238-生物工程