071010-生物化学与分子生物学
071009-细胞生物学
071011-生物物理学

内质网应激与稳态
氧化还原调控
折叠酶与分子伴侣

2004-09--2009-06   中国科学院生物物理研究所   博士
2000-09--2004-06   武汉大学生命科学学院生物学基地班   学士

   

2020-01~现在, 中国科学院生物物理研究所, 研究员
2012-01~2019-12,中国科学院生物物理研究所, 副研究员
2009-07~2011-12,中国科学院生物物理研究所, 助理研究员

2021-08-01-2025-08-01,中国生物物理学会亚细胞结构与功能分会, 副秘书长
2021-01-01-2023-12-31,中国科学院青年创新促进会, 优秀会员
2015-12-31-2019-12-30,中国科学院青年创新促进会, 会员
2014-12-31-2018-12-30,中国生理学会应激生理学专业委员会, 委员

细胞生物学实验
生物物理学实验
生物活性小分子

   

[1] 王磊, 王璐, 王曦, 王志珍. 新型靶向Ero1α/PDI电子传递系统的小分子抑制剂. CN: CN114272379A, 2022-04-05.

   

[1] Wang, Lei, Wang, Chihchen. Oxidative protein folding fidelity and redoxtasis in the endoplasmic reticulum. TRENDS IN BIOCHEMICAL SCIENCESnull. 2023, 48(1): 40-52, http://dx.doi.org/10.1016/j.tibs.2022.06.011.
[2] Cheng, Fang, Ji, Qianzhao, Wang, Lu, Wang, Chihchen, Liu, GuangHui, Wang, Lei. Reducing oxidative protein folding alleviates senescence by minimizing ER-to-nucleus H2O2 release. EMBO REPORTS[J]. 2023, 24(8): http://dx.doi.org/10.15252/embr.202256439.
[3] 胡昕炜, 王磊. “后AlphaFold时代”的蛋白质折叠问题. 科学通报[J]. 2023, 68(22): 2943-2950, [4] Yinhong Niu, Jiale Liao, Haitao Zhou, Chihchen Wang, Lei Wang, Yanli Fan. Flavonoids from Lycium barbarum Leaves Exhibit Anti-Aging Effects through the Redox-Modulation. MOLECULES[J]. 2022, 27: 4952-, https://doaj.org/article/2ec91872f191445e900c6908b4278d3f.
[5] Ping Liu, Xi Wang, Yiwei Sun, Hongyu Zhao, Fang Cheng, Jifeng Wang, Fuquan Yang, Junjie Hu, Hong Zhang, Chihchen Wang, Lei Wang. SARS-CoV-2 ORF8 reshapes the ER through forming mixed disulfides with ER oxidoreductases. REDOX BIOLOGY[J]. 2022, 54: 102388-, https://doaj.org/article/a7b3f20f43aa4354b2c610dd021420e5.
[6] Zhou, Haitao, Ding, Shanshan, Sun, Chuanxin, Fu, Jiahui, Yang, Dong, Wang, Xie, Wang, Chihchen, Wang, Lei. Lycium barbarum Extracts Extend Lifespan and Alleviate Proteotoxicity in Caenorhabditis elegans. FRONTIERS IN NUTRITION[J]. 2022, 8: 815947-, http://dx.doi.org/10.3389/fnut.2021.815947.
[7] Qiao, Xinhua, Zhang, Yingmin, Ye, Aojun, Zhang, Yini, Xie, Ting, Lv, Zhenyu, Shi, Chang, Wu, Dongli, Chu, Boyu, Wu, Xun, Zhang, Weiqi, Wang, Ping, Liu, GuangHui, Wang, Chihchen, Wang, Lei, Chen, Chang. ER reductive stress caused by Ero1 alpha S-nitrosation accelerates senescence. FREE RADICAL BIOLOGY & MEDICINE[J]. 2022, 180: 165-178, http://dx.doi.org/10.1016/j.freeradbiomed.2022.01.006.
[8] Lu Wang, Xi Wang, Xiying Lv, Qiushuo Jin, Hongcai Shang, Chih-chen Wang, Lei Wang. The extracellular Ero1α/PDI electron transport system regulates platelet function by increasing glutathione reduction potential. Redox Biology[J]. 2022, 50: 102244-, [9] Fan Fenggui, Zhang Qiao, Zhang Yini, Huang Guozhong, Liang Xuelian, Wang Chih-chen, Wang Lei, Lu Dongping. Two Arabidopsis Protein Disulfide Isomerase Subgroups Work Synergistically in Catalyzing Oxidative Protein Folding. Plant Physiology[J]. 2022, 188(1): 241-254, [10] Wan, Wang, Zeng, Lianggang, Jin, Wenhan, Chen, Xinxin, Shen, Di, Huang, Yanan, Wang, Mengdie, Bai, Yulong, Lyu, Haochen, Dong, Xuepeng, Gao, Zhenming, Wang, Lei, Liu, Xiaojing, Liu, Yu. A Solvatochromic Fluorescent Probe Reveals Polarity Heterogeneity upon Protein Aggregation in Cells. ANGEWANDTE CHEMIE-INTERNATIONAL EDITION[J]. 2021, 60(49): 25865-25871, [11] Wang, Lei, Yu, Jiaojiao, Wang, Chihchen. Protein disulfide isomerase is regulated in multiple ways: Consequences for conformation, activities, and pathophysiological functions. BIOESSAYS[J]. 2021, 43(3): https://www.webofscience.com/wos/woscc/full-record/WOS:000585977400001.
[12] Xinxin Chen, Jianchao Zhang, Pulan Liu, Yangyang Wei, Xie Wang, Junyu Xiao, Chihchen Wang, Lei Wang. Proteolytic processing of secretory pathway kinase Fam20C by site-1 protease promotes biomineralization. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA[J]. 2021, 118(32): [13] Yu Yan, Xun Wu, Pengcheng Wang, Songyang Zhang, Lulu Sun, Yang Zhao, GuangYi Zeng, Bo Liu, Guoheng Xu, Huiying Liu, Lei Wang, Xian Wang, Changtao Jiang. Homocysteine promotes hepatic steatosis by activating the adipocyte lipolysis in a HIF1α-ERO1α-dependent oxidative stress manner. REDOX BIOLOGY[J]. 2020, 37: 101742-, http://dx.doi.org/10.1016/j.redox.2020.101742.
[14] Yang, Jie, Zhang, Hong, Gong, Weibin, Liu, Zhenyan, Wu, Huiwen, Hu, Wanhui, Chen, Xinxin, Wang, Lei, Wu, Si, Chen, Chang, Perrett, Sarah. S-Glutathionylation of human inducible Hsp70 reveals a regulatory mechanism involving the C-terminal ?-helical lid. JOURNAL OF BIOLOGICAL CHEMISTRY[J]. 2020, 295(24): 8302-8324, http://dx.doi.org/10.1074/jbc.RA119.012372.
[15] Yu, Jiaojiao, Li, Tao, Liu, Yu, Wang, Xi, Zhang, Jianchao, Wang, Xie, Shi, Guizhi, Lou, Jizhong, Wang, Likun, Wang, Chihchen, VVang, Lei. Phosphorylation switches protein disulfide isomerase activity to maintain proteostasis and attenuate ER stress. EMBO JOURNAL[J]. 2020, 39(10): e103841-, https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7232009/.
[16] Fan, Fenggui, Zhang, Yini, Huang, Guozhong, Zhang, Qiao, Wang, Chihchen, Wang, Lei, Lu, Dongping. AtERO1 and AtERO2 Exhibit Differences in Catalyzing Oxidative Protein Folding in the Endoplasmic Reticulum. PLANT PHYSIOLOGY[J]. 2019, 180(4): 2022-2033, http://dx.doi.org/10.1104/pp.19.00020.
[17] Cheng, Fang, Wang, Si, Song, Moshi, Liu, Zunpeng, Liu, Ping, Wang, Lei, Wang, Yanjiang, Zhao, Qian, Yan, Kaowen, Chan, Piu, Zhang, Weiqi, Qu, Jing, Liu, GuangHui. DJ-1 is dispensable for human stem cell homeostasis. PROTEIN & CELL[J]. 2019, 10(11): 846-853, http://lib.cqvip.com/Qikan/Article/Detail?id=7100511677.
[18] Wang Lei. AtERO1 and AtERO2 exhibit differences in catalyzing oxidative protein folding in the ER. Plant Physiology. 2019, [19] Wang, Lu, Zhou, Junsong, Wang, Lei, Wang, Chihchen, Essex, David W. The b ' domain of protein disulfide isomerase cooperates with the a and a ' domains to functionally interact with platelets. JOURNAL OF THROMBOSIS AND HAEMOSTASIS[J]. 2019, 17(2): 371-382, https://www.webofscience.com/wos/woscc/full-record/WOS:000458294900015.
[20] Wu, Xun, Zhang, Lihui, Miao, Yutong, Yang, Juan, Wang, Xian, Wang, Chihchen, Feng, Juan, Wang, Lei. Homocysteine causes vascular endothelial dysfunction by disrupting endoplasmic reticulum redox homeostasis. REDOX BIOLOGY[J]. 2019, 20: 46-59, http://dx.doi.org/10.1016/j.redox.2018.09.021.
[21] Yini Zhang, Tao Li, Lihui Zhang, Fugen Shangguan, Guizhi Shi, Xun Wu, Ya Cui, Xie Wang, Xi Wang, Yongzhang Liu, Bin Lu, Taotao Wei, Chihchen Wang, Lei Wang. Targeting the functional interplay between endoplasmic reticulum oxidoreductin-1α and protein disulfide isomerase suppresses the progression of cervical cancer. EBIOMEDICINE[J]. 2019, 41: 408-419, [22] Xu, WenChang, Liang, JinZhao, Li, Cheng, He, ZhiXin, Yuan, HanYe, Huang, BenYan, Liu, XiaoLing, Tang, Bo, Pang, DaiWen, Du, HaiNing, Yang, Yi, Chen, Jie, Wang, Lei, Zhang, Min, Liang, Yi. Pathological hydrogen peroxide triggers the fibrillization of wild-type SOD1 via sulfenic acid modification of Cys-111. CELL DEATH & DISEASE[J]. 2018, 9(2): https://doaj.org/article/748fad2992e74a63af60208f55377e90.
[23] Fang, Jingqi, Yang, Jiping, Wu, Xun, Zhang, Gangming, Li, Tao, Wang, Xie, Zhang, Hong, Wang, Chihchen, Liu, GuangHui, Wang, Lei. Metformin alleviates human cellular aging by upregulating the endoplasmic reticulum glutathione peroxidase 7. AGING CELL[J]. 2018, 17(4): http://dx.doi.org/10.1111/acel.12765.
[24] Li, Huanhuan, Yang, Kai, Wang, Wenjia, Niu, Yingbo, Li, Jun, Dong, Yuhui, Liu, Yingfang, Wang, Chihchen, Wang, Lei, Liang, Huanhuan. Crystal and solution structures of human protein-disulfide isomerase-like protein of the testis (PDILT) provide insight into its chaperone activity. JOURNAL OF BIOLOGICAL CHEMISTRY[J]. 2018, 293(4): 1192-1202, http://dx.doi.org/10.1074/jbc.M117.797290.
[25] Fan, Fenggui, Zhang, Yini, Wang, Shen, Han, Yongfeng, Wang, Lei, Lu, Dongping. Characterization of the oxidative protein folding activity of a unique plant oxidoreductase, Arabidopsis protein disulfide isomerase-11. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS[J]. 2018, 495(1): 1041-1047, http://dx.doi.org/10.1016/j.bbrc.2017.11.111.
[26] Zhang, Jianchao, Zhu, Qinyu, Wang, Xie, Yu, Jiaojiao, Chen, Xinxin, Wang, Jifeng, Wang, Xi, Xiao, Junyu, Wang, Chihchen, Wang, Lei. Secretory kinase Fam20C tunes endoplasmic reticulum redox state via phosphorylation of Ero1 alpha. EMBO JOURNAL[J]. 2018, 37(14): e98699-, https://www.webofscience.com/wos/woscc/full-record/WOS:000438517100008.
[27] Li, Yazi, Zhang, Yuying, Wang, Lei, Wang, Ping, Xue, Yanhong, Li, Xiaopeng, Qiao, Xinhua, Zhang, Xu, Xu, Tao, Liu, Guanghui, Li, Peng, Chen, Chang. Autophagy impairment mediated by S-nitrosation of ATG4B leads to neurotoxicity in response to hyperglycemia. AUTOPHAGY[J]. 2017, 13(7): 1145-1160, https://www.webofscience.com/wos/woscc/full-record/WOS:000406244200005.
[28] Niu, Yingbo, Zhang, Lihui, Yu, Jiaojiao, Wang, Chihchen, Wang, Lei. Novel Roles of the Non-catalytic Elements of Yeast Protein-disulfide Isomerase in Its Interplay with Endoplasmic Reticulum Oxidoreductin 1. JOURNAL OF BIOLOGICAL CHEMISTRY[J]. 2016, 291(15): 8283-8294, http://dx.doi.org/10.1074/jbc.M115.694257.
[29] Wang, Lei, Wang, Xi, Wang, Chihchen. Protein disulfide-isomerase, a folding catalyst and a redox-regulated chaperone. FREE RADICAL BIOLOGY AND MEDICINE[J]. 2015, 83: 305-313, http://dx.doi.org/10.1016/j.freeradbiomed.2015.02.007.
[30] 王曦, 王磊, 陈畅, 王志珍. 未折叠蛋白响应：2014年拉斯克基础医学研究奖解读. 科学通报[J]. 2014, 59(36): 3533-3534, https://d.wanfangdata.com.cn/periodical/kxtb201436001.
[31] Zhang, Lihui, Niu, Yingbo, Zhu, Li, Fang, Jingqi, Wang, Xie, Wang, Lei, Wang, Chihchen. Different Interaction Modes for Protein-disulfide Isomerase (PDI) as an Efficient Regulator and a Specific Substrate of Endoplasmic Reticulum Oxidoreductin-1 alpha (Ero1 alpha). JOURNAL OF BIOLOGICAL CHEMISTRY[J]. 2014, 289(45): 31188-31199, http://dx.doi.org/10.1074/jbc.M114.602961.
[32] Wang, Lei, Zhang, Lihui, Niu, Yingbo, Sitia, Roberto, Wang, Chihchen. Glutathione Peroxidase 7 Utilizes Hydrogen Peroxide Generated by Ero1 alpha to Promote Oxidative Protein Folding. ANTIOXIDANTS & REDOX SIGNALING[J]. 2014, 20(4): 545-556, http://dx.doi.org/10.1089/ars.2013.5236.
[33] Lihui Zhang, Yingbo Niu, Li Zhu, Jingqi Fang, Xie Wang, Lei Wang, Chihchen Wang. Different Interaction Modes for Protein-disulfide Isomerase (PDI) as an Efficient Regulator and a Specific Substrate of Endoplasmic Reticulum Oxidoreductin-1α (Ero1α) *. THE JOURNAL OF BIOLOGICAL CHEMISTRY. 2014, 289(45): 31188-31199, http://dx.doi.org/10.1074/jbc.M114.602961.
[34] Wang, Chao, Yu, Jiang, Huo, Lin, Wang, Lei, Feng, Wei, Wang, Chihchen. Human Protein-disulfide Isomerase Is a Redox-regulated Chaperone Activated by Oxidation of Domain a '. JOURNAL OF BIOLOGICAL CHEMISTRY[J]. 2012, 287(2): 1139-1149, http://dx.doi.org/10.1074/jbc.M111.303149.
[35] Wang, Lei, Zhu, Li, Wang, Chihchen. The endoplasmic reticulum sulfhydryl oxidase Ero1 beta drives efficient oxidative protein folding with loose regulation. BIOCHEMICAL JOURNAL[J]. 2011, 434: 113-121, http://dx.doi.org/10.1042/BJ20101357.
[36] Nguyen, Van Dat, Saaranen, Mirva J, Karala, AnnaRiikka, Lappi, AnnaKaisa, Wang, Lei, Raykhel, Irina B, Alanen, Heli I, Salo, Kirsi E H, Wang, Chihchen, Ruddock, Lloyd W. Two Endoplasmic Reticulum PDI Peroxidases Increase the Efficiency of the Use of Peroxide during Disulfide Bond Formation. JOURNAL OF MOLECULAR BIOLOGY[J]. 2011, 406(3): 503-515, http://dx.doi.org/10.1016/j.jmb.2010.12.039.
[37] Cheng, Han, Wang, Lei, Wang, Chihchen. Domain a' of protein disulfide isomerase plays key role in inhibiting alpha-synuclein fibril formation. CELL STRESS & CHAPERONES[J]. 2010, 15(4): 415-421, http://dx.doi.org/10.1007/s12192-009-0157-2.
[38] Wang, Lei, Li, Shengjian, Sidhu, Ateesh, Zhu, Li, Liang, Yi, Freedman, Robert B, Wang, Chihchen. Reconstitution of Human Ero1-L alpha/Protein-Disulfide Isomerase Oxidative Folding Pathway in Vitro POSITION-DEPENDENT DIFFERENCES IN ROLE BETWEEN THE a AND a ' DOMAINS OF PROTEIN-DISULFIDE ISOMERASE. JOURNAL OF BIOLOGICAL CHEMISTRY[J]. 2009, 284(1): 199-206, http://dx.doi.org/10.1074/jbc.M806645200.
[39] Wang, Likun, Wang, Lei, Vavassori, Stefano, Li, Shengjian, Ke, Huimin, Anelli, Tiziana, Degano, Massimo, Ronzoni, Riccardo, Sitia, Roberto, Sun, Fei, Wang, Chihchen. Crystal structure of human ERp44 shows a dynamic functional modulation by its carboxy-terminal tail. EMBO REPORTS[J]. 2008, 9(7): 642-647, http://www.irgrid.ac.cn/handle/1471x/757645.

   

（ 1 ） 内质网氧化还原稳态在高同型半胱氨酸血症引起血管内皮细胞功能损伤中的作用, 主持, 国家级, 2018-01--2021-12
（ 2 ） Ero1α翻译后修饰介导的内质网氧化应激及抗氧化应答, 主持, 国家级, 2016-01--2019-12
（ 3 ） 细胞器动态互作的蛋白质机器, 参与, 国家级, 2016-07--2021-06
（ 4 ） 基于糖尿病发生进程关键信号通路的分子靶标的发现与确认, 参与, 部委级, 2016-01--2017-12
（ 5 ） 细胞分泌途径关键大分子复合体的跨尺度研究, 主持, 部委级, 2020-01--2024-12
（ 6 ） 内质网中蛋白质氧化折叠及其调控的新机制, 主持, 国家级, 2014-01--2017-12
（ 7 ） 人细胞内质网中巯基氧化酶Ero1β驱动的电子传递系统的工作机制, 主持, 国家级, 2011-01--2013-12
（ 8 ） 内质网稳态调节, 主持, 国家级, 2021-01--2023-12