071010-生物化学与分子生物学
071005-微生物学

蛋白质晶体学、蛋白质工程
微生物学，生物催化

2005-09--2010-06   南京农业大学   博士
2001-09--2005-06   南京农业大学   学士

   

2020-07~现在, 中国科学院天津工业生物技术研究所, 研究员
2020-02~2021-01,Greifswald University (Germany), 访问学者
2014-04~2020-06,中国科学院天津工业生物技术研究所, 副研究员
2010-07~2014-04,中国科学院天津工业生物技术研究所, 助理研究员
2005-09~2010-06,南京农业大学, 博士
2001-09~2005-06,南京农业大学, 学士

   

（1） 结构生物学在酶的功能研究中的应用, 二等奖, 省级, 2019

（ 1 ） 高活性PET水解酶突变体及其应用, 2021, 第 2 作者, 专利号: CN111057693B

（ 2 ） 内酯酶及利用内酯酶降解α-玉米赤霉烯醇的方法, 2017, 第 6 作者, 专利号: CN106929493A

（ 3 ） 一种利用共固定化酶合成D-丙氨酸的方法, 2016, 第 4 作者, 专利号: CN106191150A

（ 4 ） 嗜热共生杆菌meso-二氨基庚二酸脱氢酶突变体应用, 2016, 第 2 作者, 专利号: CN105821090A

（ 5 ） 嗜热共生杆菌meso-二氨基庚二酸脱氢酶突变体, 2016, 第 1 作者, 专利号: CN105821014A

（ 6 ） D-叔亮氨酸的一种生物催化制备方法, 2015, 第 1 作者, 专利号: CN104513839A

（ 7 ） 合成D-氨基酸的一种新方法, 2014, 第 5 作者, 专利号: CN103667380A

（ 8 ） 纤维素水解酶β-1,4葡聚糖内切酶基因, 2010, 第 4 作者, 专利号: CN101736023A

   

（1） Characterization of a PBAT Degradation Carboxylesterase from Thermobacillus composti KWC4, CATALYSTS, 2023, 第 9 作者
（2） 聚对苯二甲酸乙二醇酯（PET）塑料水解酶结构、功能及改造, Sructure,function and application of hydrolases for polyethylene terephthalate(PET)degradation, 生物加工过程, 2022, 第 5 作者
（3） Multiple Substrate Binding Mode-Guided Engineering of a Thermophilic PET Hydrolase, ACS CATALYSIS, 2022, 通讯作者
（4） Structural Insights into (Tere)phthalate-Ester Hydrolysis by a Carboxylesterase and Its Role in Promoting PET Depolymerization, ACS CATALYSIS, 2022, 通讯作者
（5） A Combined Computational-Experimental Study on the Substrate Binding and Reaction Mechanism of Salicylic Acid Decarboxylase, CATALYSTS, 2022, 第 9 作者
（6） Structural insight and engineering of a plastic degrading hydrolase Ple629, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2022, 通讯作者
（7） Structural insights to a bi-functional isoprenyl diphosphate synthase that can catalyze head-to-tail and head-to-middle condensation, INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2022, 第 7 作者
（8） Substrate-Binding Mode of a Thermophilic PET Hydrolase and Engineering the Enzyme to Enhance the Hydrolytic Efficacy, ACSCATALYSIS, 2022, 第 6 作者
（9） Molecular Insights into the Regioselectivity of the Fe(II)/2-Ketoglutarate-Dependent Dioxygenase-Catalyzed C-H Hydroxylation of Amino Acids, ACS CATALYSIS, 2022, 通讯作者
（10） Mechanism-Based Design of Efficient PET Hydrolases, ACS CATALYSIS, 2022, 第 6 作者
（11） Protein Engineering of Glucosylglycerol Phosphorylase Facilitating Efficient and Highly Regio- and Stereoselective Glycosylation of Polyols in a Synthetic System, ACS CATALYSIS, 2022, 通讯作者
（12） Unlocking the stereoselectivity and substrate acceptance of enzymes: Proline induced loop engineering test, Angewandte Chemie International Edition, 2022, 第 6 作者
（13） A Structural and Bioinformatics Investigation of a Fungal Squalene Synthase and Comparisons with Other Membrane Proteins, ACS OMEGA, 2022, 第 3 作者
（14） Molecular and Biochemical Differences of the Tandem and Cold-Adapted PET Hydrolases Ple628 and Ple629, Isolated From a Marine Microbial Consortium, FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY, 2022, 第 4 作者
（15） Engineered Imine Reductase for Larotrectinib Intermediate Manufacture, ACS CATALYSIS, 2022, 第11作者
（16） Computational Redesign of a PETase for Plastic Biodegradation under Ambient Condition by the GRAPE Strategy, ACS CATALYSIS, 2021, 第11作者
（17） Terpene Cyclases and Prenyltransferases: Structures and Mechanisms of Action, ACS CATALYSIS, 2021, 第 4 作者
（18） General features to enhance enzymatic activity of poly(ethylene terephthalate) hydrolysis, NATURE CATALYSIS, 2021, 第 7 作者
（19） Crystal Structures and Catalytic Mechanism of l-erythro-3,5-Diaminohexanoate Dehydrogenase and Rational Engineering for Asymmetric Synthesis of beta-Amino Acids, ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2021, 第 8 作者
（20） Structural Basis of Salicylic Acid Decarboxylase Reveals a Unique Substrate Recognition Mode and Access Channel, JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY, 2021, 第 7 作者
（21） Near-perfect control of the regioselective glucosylation enabled by rational design of glycosyltransferases, GREEN SYNTHESIS AND CATALYSIS, 2021, 第 6 作者
（22） 2,3-Dihydroxybenzoic Acid Decarboxylase fromFusarium oxysporum: Crystal Structures and Substrate Recognition Mechanism, CHEMBIOCHEM, 2020, 通讯作者
（23） Totally atom-economical synthesis of lactic acid from formaldehyde: combined bio-carboligation and chemo-rearrangement without the isolation of intermediates, GREEN CHEMISTRY, 2020, 第 9 作者
（24） Efficient O-Glycosylation of Triterpenes Enabled by Protein Engineering of Plant Glycosyltransferase UGT74AC1, ACS CATALYSIS, 2020, 通讯作者
（25） Structure of an antibiotic-synthesizing UDP-glucuronate 4-epimerase MoeE5 in complex with substrate, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2020, 第 4 作者
（26） 来源于麦氏喙枝孢霉的玉米赤霉烯酮水解酶在毕赤酵母中的高效表达及活性分析, High expression and characterization of a novel zearalenone hydrolase from Rhinocladiella mackenziei in Pichia pastoris, 微生物学通报, 2020, 第 3 作者
（27） Structure of a gut microbial diltiazem-metabolizing enzyme suggests possible substrate binding mode, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2020, 第 4 作者
（28） Biochemical characterization and structural analysis of ulvan lyase from marine Alteromonas sp . reveals the basis for its salt tolerance, INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, 2020, 通讯作者
（29） Complex structures of MoeN5 with substrate analogues suggest sequential catalytic mechanism, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2019, 第 4 作者
（30） Crystal structure and proposed mechanism of an enantioselective hydroalkoxylation enzyme from Penicillium herquei, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2019, 第 4 作者
（31） Structural insights to heterodimeric cis-prenyltransferases through yeast dehydrodolichyl diphosphate synthase subunit Nus1, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2019, 第 8 作者
（32） Efficient reductive desymmetrization of bulky 1,3-cyclodiketones enabled by structure-guided directed evolution of a carbonyl reductase, NATURE CATALYSIS, 2019, 第 4 作者
（33） Crystal structure of LepI, a multifunctional SAM-dependent enzyme which catalyzes pericyclic reactions in leporin biosynthesis, ORGANIC & BIOMOLECULAR CHEMISTRY, 2019, 第 7 作者
（34） Functional and structural investigations of fibronectin-binding protein Apa from Mycobacterium tuberculosis, BIOCHIMICA ET BIOPHYSICA ACTA-GENERAL SUBJECTS, 2019, 第 7 作者
（35） Functional and structural investigations of fibronectin-binding protein Apa from Mycobacterium tuberculosis, BBA - GENERAL SUBJECTS, 2019, 第 7 作者
（36） 黑曲霉内切葡聚糖酶AnCel5A的表达纯化与晶体优化, Expression,Purification and Crystallization of a Glycoside Hydrolase Family 5 Endoglucanase(AnCel5A) from Aspergillus niger, 食品与生物技术学报, 2019, 第 7 作者
（37） 工业酶结构与功能的构效关系, Structure-function relationships of industrial enzymes, 生物工程学报, 2019, 第 3 作者
（38） 玉米赤霉烯酮水解酶耐热性的分子改造, Molecular Engineering of Mycoestrogen-Detoxifying Lactonase ZHD101 to Improve Enzyme Thermostability, 食品与生物技术学报, 2019, 第 3 作者
（39） Discovery of Lipophilic Bisphosphonates that Target Bacterial Cell Wall and Quinone Biosynthesis, JOURNAL OF MEDICINAL CHEMISTRY, 2019, 第 5 作者
（40） Substrate-analogue complex structure of Mycobacterium tuberculosis decaprenyl diphosphate synthase, ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 2019, 第 5 作者
（41） New views of head-to-middle prenyltransferases, ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY, 2018, 第 6 作者
（42） Crystal Structure of a Mycoestrogen-Detoxifying Lactonase from Rhinocladiella mackenziei: Molecular Insight into ZHD Substrate Selectivity, ACS CATALYSIS, 2018, 第 5 作者
（43） Structural studies reveal the molecular mechanism of PETase, FEBS JOURNAL, 2018, 通讯作者
（44） Structural insight into a novel indole prenyltransferase in hapalindole-type alkaloid biosynthesis, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2018, 第 4 作者
（45） 来源于 Rhinocladiella mackenziei 的玉米赤霉烯酮水解酶的表达纯化与酶学性质, Expression, purification and characterization of a novel zearalenone hydrolase from Rhinocladiella mackenziei, 微生物学通报, 2018, 第 3 作者
（46） 金黄色葡萄球菌脱水鲨烯脱氢酶（CrtN）和DNA结合蛋白Sso7d的融合表达, Fusion Expression of Dehydrosqualene Desaturase(CrtN)from Staphylococcus aureus and DNA Binding Protein Sso7d, 天津科技大学学报, 2018, 第 5 作者
（47） Enzymatic synthesis of d-alanine from a renewable starting material by co-immobilized dehydrogenases, PROCESS BIOCHEMISTRY, 2018, 第 4 作者
（48） Catalytic Role of Conserved Asparagine, Glutamine, Serine, and Tyrosine Residues in Isoprenoid Biosynthesis Enzymes, ACS CATALYSIS, 2018, 第 5 作者
（49） Crystal structure of p-nitrophenol 4-monooxygenase PnpA from Pseudomonas putida DLL-E4: The key enzyme involved in p-nitrophenol degradation, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2018, 通讯作者
（50） Crystal structure of hydroxyquinol 1,2-dioxygenase PnpC from Pseudomonas putida DLL-E4 and its role of N-terminal domain for catalysis, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2018, 第 6 作者
（51） "Head-to-Middle" and "Head-to-Tail" cis-Prenyl Transferases: Structure of Isosesquilavandulyl Diphosphate Synthase, ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2018, 第 8 作者
（52） The Crystal Structure of a Class of Cyclases that Catalyze the Cope Rearrangement, ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2018, 通讯作者
（53） Characterization and crystal structure of a thermostable glycoside hydrolase family 45 1,4-beta-endoglucanase from Thielavia terrestris, ENZYME AND MICROBIAL TECHNOLOGY, 2017, 第 4 作者
（54） Characterization and crystal structure of a novel zearalenone hydrolase from Cladophialophora bantiana, ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 2017, 第 4 作者
（55） Structural insight into catalytic mechanism of PET hydrolase, NATURE COMMUNICATIONS, 2017, 第 2 作者
（56） Characterization and crystal structure of a novel zearalenone hydrolase from Cladophialophora bantiana, ACTA CRYSTALLOGRAPHICA SECTION F-STRUCTURAL BIOLOGY COMMUNICATIONS, 2017, 第 4 作者
（57） Head-to-Head Prenyl Synthases in Pathogenic Bacteria, CHEMBIOCHEM, 2017, 第 3 作者
（58） Structural insight into potential cold adaptation mechanism through a psychrophilic glycoside hydrolase family 10 endo-beta-1,4-xylanase, JOURNAL OF STRUCTURAL BIOLOGY, 2016, 第 3 作者
（59） Crystal structure and genetic modifications of FI-CMCase from Aspergillus aculeatus F-50, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2016, 第 2 作者
（60） Functional and structural analyses of a 1,4-beta-endoglucanase from Ganoderma lucidum, ENZYME AND MICROBIAL TECHNOLOGY, 2016, 第 6 作者
（61） Moenomycin Biosynthesis: Structure and Mechanism of Action of the Prenyl Transferase MoeN5, ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2016, 第 6 作者
（62） Structure, Function, and Inhibition of Staphylococcus aureus Heptaprenyl Diphosphate Synthase, CHEMMEDCHEM, 2016, 第 4 作者
（63） Enhanced alpha-Zearalenol Hydrolyzing Activity of a Mycoestrogen-Detoxifying Lactonase by Structure-Based Engineering, ACS CATALYSIS, 2016, 第 2 作者
（64） Structures of Trypanosome Vacuolar Soluble Pyrophosphatases: Antiparasitic Drug Targets, ACS CHEMICAL BIOLOGY, 2016, 第 6 作者
（65） Functional and structural analysis of Pichia pastoris-expressed Aspergillus niger 1,4-beta-endoglucanase, BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS, 2016, 第 2 作者
（66） Structure and Function of a "Head-to-Middle" Prenyltransferase: Lavandulyl Diphosphate Synthase, ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2016, 第 7 作者
（67） Structures of Iridoid Synthase from Cantharanthus roseus with Bound NAD(+), NADPH, or NAD(+)/10-Oxogeranial: Reaction Mechanisms, ANGEWANDTE CHEMIE-INTERNATIONAL EDITION, 2015, 第 2 作者
（68） 嗜热共生杆菌内消旋-2，6-二氨基庚二酸脱氢酶中Y76对辅酶偏好性影响, Effect of residue Y76 on co-enzyme specificity of meso-diaminopimelate dehydrogenase from Symbiobacterium thermophilum, 生物工程学报, 2015, 第 2 作者
（69） Structural Analysis Reveals the Substrate-Binding Mechanism for the Expanded Substrate Specificity of Mutant meso-Diaminopimelate Dehydrogenase, CHEMBIOCHEM, 2015, 第 1 作者
（70） Structural and Mutational Studies on the Unusual Substrate Specificity of meso-Diaminopimelate Dehydrogenase from Symbiobacterium thermophilum, CHEMBIOCHEM, 2014, 第 1 作者
（71） Highly enantioselective double reduction of phenylglyoxal to (R)-1-phenyl-1,2-ethanediol by one NADPH-dependent yeast carbonyl reductase with a broad substrate profile, TETRAHEDRON, 2013, 第 2 作者
（72） 一种来源于毕赤酵母的高对映选择性羰基还原酶 的性质及底物谱分析, 生物工程学报, 2013, 第 2 作者
（73） 不对称还原大位阻二芳香基甲酮的羰基还原酶的基 因克隆及性质分析, 生物工程学报, 2013, 第 2 作者
（74） 茄子种质资源分类研究进展, 中国蔬菜, 2012, 第 2 作者
（75） A Novel meso-Diaminopimelate Dehydrogenase from Symbiobacterium thermophilum: Overexpression, Characterization, and Potential for D-Amino Acid Synthesis, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2012, 第 3 作者
（76） Cloning and characterization of a gene cluster involved in the catabolism of p-nitrophenol from Pseudomonas putida DLL-E4, BIORESOURCE TECHNOLOGY, 2010, 第 2 作者
（77） 高效除磷工程菌Pseudomonas putida GM6-PPK1的构建及其除磷能力研究, Enhancement of Phosphorus Removal by Overexpression of Polyphosphate Kinase 1 in Pseudomonas Putida GM6, 土壤, 2009, 第 2 作者
（78） 重组有机磷水解酶MPH的发酵条件的优化研究, Optimization of fermentation condition for remobilization organophosphate hydrolase MPH, 工业微生物, 2007, 第 3 作者
（79） 邻单胞菌M6中甲基对硫磷水解酶（MPH）的纯化及性质的研究, Purification and characterization of methyl parathion hydrolase (MPH) from Plesiomonas sp. M6, 高技术通讯, 2006, 第 3 作者
（80） 甲基对硫磷水解酶参与催化相关结构的研究, Determination of the Catalytic Structures of Methyl Parathion Hydrolase, 生物工程学报, 2005, 第 2 作者
（81） 生物表面活性剂产生菌的分离培养及其产物特性研究, ISOLATION OF BIOSURFACTANT PRODUCING BACTERIA AND CHARACTERISTICS OF THE BIOSURFACTANT, 土壤, 2004, 第 2 作者
（82） 一氧化氮供体硝普钠降低肉鸡肺动脉压试验研究, Study on Reduction of Pulmonary Arterial Pressure by Sodium Nitroprusside in Broilers, 畜牧兽医学报, 2002, 第 5 作者

Structural analysis of PET-degrading enzymes PETase and MHETase from Ideonella sakaiensis, Elsevier B.V., 2021-01, 第 4 作者

   

（ 1 ） L-氨基酸脱氢酶手性选择性改造及机理研究, 负责人, 国家任务, 2012-01--2014-12
（ 2 ） 生物催化不对称羰基还原胺化反应的探索, 参与, 国家任务, 2011-01--2013-12
（ 3 ） 生物合成的手性识别机理解释, 参与, 国家任务, 2011-01--2015-08
（ 4 ） 丙酮酸的生物合成与衍生转化, 参与, 中国科学院计划, 2013-01--2015-12
（ 5 ） 结核分枝杆菌十异戊二烯焦磷酸合成酶功能研究及抗结核药物开发, 负责人, 国家任务, 2016-01--2019-12
（ 6 ） 难降解有毒污染物降解酶的晶体结构解析, 负责人, 国家任务, 2019-07--2024-06
（ 7 ） 受体蛋白和核酸酶的结构重塑与功能拓展, 负责人, 国家任务, 2021-12--2026-11
（ 8 ） 工业蛋白质研究与服务平台, 负责人, 地方任务, 2021-11--2024-10