Heavy-ion radiation biology, Apoptosis, DNA damage, Alternative splicing, Melecular biology  of tumor

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​Research Interests and Activities

★Application of antibody array of splicing isoforms

It has been shown that most human genes are alternatively spliced and defects in alternative splicing (AS) contribute to cancer. However, currently, antibody microarrays are not used to study alternative splicing, which enhances proteome diversity and also modulate cancer-related proteins. Here we present an approach that allows the generation of an antibody microarray for the analysis of the expression of splice variants associated with cancer.

★p73 isoforms and radiation-induced apoptosis

p73, has two distinct promoters, which allow the formation of two protein isoforms: full-length transactivating (TA) p73 and an N-terminally truncated p73 species (referred to as DNp73) that lacks the N-terminal transactivating domain.Our findings have shown that there is a differentialΔNp73 expression in response to different LET radiations,and downregulatedΔNp73 expression might play a critical role in sensitivity of tumor cells

  ★PCBP1 and p73 isoforms in cancer cells exposed to ionizing radiation

Based on the research ideal combined radiation and gene therapy, this project focused on the tumor hotspot of PCBP1，as a bridge protein, has been found related to malignancy. PCBP1 expression regulates p73 isoforms expression and sensitizes cancer cells to ionizing radiation，which is explored here. The aim of this project is to explore the radiation-induced tumor cell apoptosis molecular mechanism regulated by PCBP1.

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Awards

² 2014, Japan-Asia Youth Exchange Program in Science

² 2008-2010, Helmholtz – CSC – Post doc Fellowship

² 2012, Travel FellowshipAward– PTCOG51

Funds and projects

² 2013-2015, National Natural Science Foundation of China (No.11205219)

² 2013-2015, West Light Foundation of the Chinese Academy of Sciences (Y260230XB0)

² 2016-2020,National Natural Science Foundation of China (No.11175222)

² 2016-2020,Ministry of science and technology national key R & D project(2016YFC0904602)

1.  Cuixia Di，Lu Han, Hong Zhang, Shuai Xu, Aihong MAO, et al. Dially disulfide attenuated carbon ion irradiation induced apoptosis in mouse testis through changing the ratio of Tap73/ΔNp73 via mitochondrial pathway. Scientific reports. 2015. DOI:10.1038/srep16020. （IF: 5.58）
2.  Cuixia Di，Chao Sun, Hongyan Li, Jing Si, Hong Zhang et al. Diallyl disulfide enhances carbon ion beams–induced apoptotic cell death in cervical cancer cells through regulating Tap73 /ΔNp73. Cell Cycle.2015,14(23):3725-33（IF:4.57）
3.  Cuixia Di, Lina Yang, Hong Zhang, Xiaofei Ma, Xin Zhang,et al. Mechanisms, function and clinical applications of DNp73. Cell Cycle. 2013,12(12):1861-67 .（IF: 4.57）
4.  Cuixia Di, Lina Yang, Hong Zhang, Lizhe An, Xin Zhang, Xiaofei Ma et al. Anti-Apoptotic ΔNp73 Degraded by Ionizing Irradiation of Different LET in Hela Cells. GENE.  2013,515(1):208-13 .（IF: 2.341）
5.  Cuixia Di, Hong Zhang, Zhenglong Sun, Honglei Jia, Lina Yang,et al. Spatial Distribution of Polygalacturonase Inhibiting proteins in Arabidopsis and Their Expression Induced by Stemphylium solani infection. GENE.  2013,515(1):208-13 .（IF: 2.341）
6.  Cuixia Di, Ming Li, Feng Long, Muqun Bai, Yajie liu, et al. Molecular cloning, functional analysis and localization of a novel gene encoding PGIPin Chorispora bungeana. Planta.2009,23(1):169-178.（IF: :3.26）
7.  Cuixia Di, Manxiao Zhang, Shijian Xu, Tuo Cheng, Lizhe An. Role of Poly-galacturonase Inhibiting Protein in
Plant Defense. Critical Reviews in Microbiology. 2006, 32(2):91-100. （IF: 6.02
8.  Hongyan Li, Hong Zhang, Cuixia Di, et al. Comparative proteomic profiling and possible toxicological mechanism of acute injury induced by carbon ion radiation in pubertal mice testes. Reproductive
Toxicology.2015. DOI:10.1016/j.reprotox.2015.07.079. （IF: 3.23）
9.  Aihong Mao, Yang Liu, Hong Zhang, Cuixia Di, et al. MicroRNA Expression and Biogenesis in Cellular Response to Ionizing Radiation. DNA and Cell Biology. 2014, 33(10).· （IF: 2.06）
10.  Mao A, Liu Y, Wang Y, Zhao Q, Zhou X, Sun C, Di Cuixia, Si J, Gan L, Zhang H. miR-449a enhances radiosensitivity through modulating pRb/E2F1 in prostate cancer cells. Tumour Biol. 2015.
11.  Zhou xin, Yupei Wang, Jing Si, Rong Zhou, Lu Gan. Cuixia Di. Laser controlled singlet oxygen generation in mitochondria to promote mitochondrial DNA replication in vitro. Scitific reports. 2015. DOI:10.1038/srep16925.
（IF: 5.58）
12. Sun C, Wang ZH, Liu Y, Liu YY, Li HY, Cuixia Di, et al. Carbon Ion Beams Induce Hepatoma Cell Death by NADPH Oxidase-mediated Mitochondrial Damage. Journal of Cellular Physiology. 2014,
229(1): 100-107
13. Yuanyuan Liu, Yang liu, Hong Zhang, Chao Sun , Queyue Zhao, Cuixia Di, et al. Effects of carbon-ion beam
irradiation on the angiogenic response in lung adenocarcinoma A549 cells. Cell Biology International. 2014, 38(11): 1304-1310
14. Li HY, Xie Y, Zhang H, Liu Y, Sun C, Liu YY, Di CuiXia, Miao GY, Zhang X,Zhou X, Ma XF, Xu S, Gan L. Simulated microgravity conditions and carbonion irradiation-induced spermatogenic cells apoptosis and sperm DNA damage. Biomedical and Environmental Sciences. 2013, 26(9): 726-734
15. Li HY, Zhang H, Xie Y, He YX, Miao GY, Yang LN, Di Cuixia, He Y. Proteomicanalysis for testis of mice exposed to carbon ion radiation. Mutat Res. 2013, 755:148-155
16.  Si Jing, Zhang Hong, Wang Zhenhua, Wu Zhenhua, Lu Jiang, Di Cuixia, Zhou Xin. Effects of 12C6+ ion radiation and ferulic acid on the zebrafish (Danio rerio) embryonic oxidative stress response and gene expression. Mutant research. 2013.745-746.（IF: 2.85）
17.  Wu Z, Wang X, Yang R, Liu Y, Zhao W, Si J, Ma X, Sun C, Liu Y, Tan Y, Liu W, Zhang X, Di Cuixia, Wang Z, Zhang H, Zhang Z. Effects of carbon ion beam irradiation on lung injury and pulmonary fibrosis in mice. Exp Ther Med. 2013. 5(3):771-776. （IF: 0.85）
18.  Yang Liu, Luwei Zhang, Yuanyuan Liu, Chao sun,· Hong Zhang, Guoying Miao, Cui Xiao Di. DNA-PKcs Deficiency Inhibits Glioblastoma Cell-Derived Angiogenesis After Ionizing Radiation. Journal of cellular physiology.2014
19.  Yajie Liu · Zhiguang Zhao · Jing Si · Cuixia Di .Brassinosteroids alleviate chilling-induced oxidative damage by enhancing antioxidant defense system in suspension cultured cells of Chorispora bungeana.Plant Growth Regulation. 2009. 59(3):207-214
20.  Yong Zhang. Tong-Wen Yang. Li-Jing Zhang. Teng-Guo Zhang. Cuixia Di. Isolation and Expression Analysis of Two Cold ‐Inducible Genes Encoding Putative CBFTranscription Factors from Chinese Cabbage. Journal of Integrative Plant Biology. 2006. 48(7):848 - 856.

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1. Study the molecular mechanism of carbon ion radiotherapy

2.Creation an new way to detect splicing isoform related to cancer development or cancar therapy.

3. Study the melecular role of p73 in  cancer.

4. Find the mechanism how splicing factor regulating isoform expression

1. 2009. 10th EMBL/EMBO Joint Conference on Science and Society. Heidelberg, Germany (Attendance).
2.  2010. 12th Status Seminar Chip Technologies, Sequencing and Functional Genomics. Frankfurt, Germany (Poster and Abstract submission).
3.  2010. 3rd Annual Meeting of NGFN‐Plus and NGFN‐Transfer in the Program of Medical Genome Research. FU Berlin，Germany. (Oral Presentation)
4.  2010. Work shop: GMP production as a prerequisite of translational research. Heidelberg, Germany. (Oral Presentation)
5.  2012. 51st Annual Meeting of Particle Therapy Co-Operative Group (PTCOG51). Seoul, Korea (Poster and Abstract submission)

1. German Cancer Research Center (DKFZ), Germany.

2. Lanzhou University, China