071010-生物化学与分子生物学
086000-生物与医药

表观遗传学的结构生物学研究
表观遗传学的结构生物学研究

1999-09--2005-01   清华大学   博士学位
1995-09--1999-07   清华大学   学士学位

   

2012-08~现在, 上海生命科学研究院生化细胞所, 研究员
2005-05~2012-08,美国密歇根大学, 博士后

bio2000 分子生物学

   

（1） 中国科学院优秀研究生导师奖, 院级, 2016

   

[1] Rekha Rai, Kevin Biju, Wenqi Sun, Tori Sodeinde, Amer AlHiysat, Jaida Morgan, Xianwen Ye, Xueqing Li, Yong Chen, Sandy Chang. Homology directed telomere clustering, ultrabright telomere formation and nuclear envelope rupture in cells lacking TRF2B and RAP1. NATURE COMMUNICATIONS[J]. 2023, 14(1): 1-20, https://doaj.org/article/797c086c8a774aefa0c7a219423bf7b6.
[2] Li, Yanjing, Zhao, Lijie, Zhang, Yuebin, Wu, Ping, Xu, Ying, Mencius, Jun, Zheng, Yongxin, Wang, Xiaoman, Xu, Wancheng, Huang, Naizhe, Ye, Xianwen, Lei, Ming, Shi, Pan, Tian, Changlin, Peng, Chao, Li, Guohui, Liu, Zhijun, Quan, Shu, Chen, Yong. Structural basis for product specificities of MLL family methyltransferases. MOLECULAR CELL[J]. 2022, 82(20): 3810-+, http://dx.doi.org/10.1016/j.molcel.2022.08.022.
[3] Li, Yanjing, Ge, Kexue, Li, Tingting, Cai, Run, Chen, Yong. The engagement of histone lysine methyltransferases with nucleosomes: structural basis, regulatory mechanisms, and therapeutic implications. PROTEIN & CELL. 2022, http://dx.doi.org/10.1093/procel/pwac032.
[4] Wang, Duo, Yan, Fuxiang, Wu, Ping, Ge, Kexue, Li, Muchun, Li, Tingting, Gao, Ying, Peng, Chao, Chen, Yong. Global profiling of regulatory elements in the histone benzoylation pathway. NATURE COMMUNICATIONS[J]. 2022, 13(1): http://dx.doi.org/10.1038/s41467-022-29057-2.
[5] Lijie Zhao, Naizhe Huang, Jun Mencius, Yanjing Li, Ying Xu, Yongxin Zheng, Wei He, Na Li, Jun Zheng, Min Zhuang, Shu Quan, Yong Chen. DPY30 acts as an ASH2L-specific stabilizer to stimulate the enzyme activity of MLL family methyltransferases on different substrates. ISCIENCE[J]. 2022, 25(9): https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9440282/.
[6] Zheng, Yongxin, Huang, Yinping, Mencius, Jun, Li, Yanjing, Zhao, Lijie, Luo, Wanting, Chen, Yong, Quan, Shu. Distinct kinetic mechanisms of H3K4 methylation catalyzed by MLL3 and MLL4 core complexes. JOURNAL OF BIOLOGICAL CHEMISTRY[J]. 2021, 296: http://dx.doi.org/10.1016/j.jbc.2021.100635.
[7] Peili Gu, Shuting Jia, Taylor Takasugi, Valerie M Tesmer, Jayakrishnan Nandakumar, Yong Chen, Sandy Chang. Distinct functions of POT1 proteins contribute to the regulation of telomerase recruitment to telomeres. NATURE COMMUNICATIONS[J]. 2021, 12(1): 1-16, [8] Ban, Hyoju, Sun, Wenqi, Chen, YuHang, Chen, Yong, Li, Fei. Dri1 mediates heterochromatin assembly via RNAi and histone deacetylation. GENETICS[J]. 2021, 218(1): http://dx.doi.org/10.1093/genetics/iyab032.
[9] Guo, Tianyue, Dong, Yao, Chen, Yong, Liu, Liu, Sun, Haiying. Development and optimization of a cascade of screening assays for inhibitors of TRF2. ANALYTICAL BIOCHEMISTRY[J]. 2020, 602: http://dx.doi.org/10.1016/j.ab.2020.113796.
[10] Chen, Guochao, Li, Wei, Yan, Fuxiang, Wang, Duo, Chen, Yong. The Structural Basis for Specific Recognition of H3K14 Acetylation by Sth1 in the RSC Chromatin Remodeling Complex. STRUCTURE[J]. 2020, 28(1): 111-+, http://dx.doi.org/10.1016/j.str.2019.10.015.
[11] Li, Yanjing, Zhao, Lijie, Tian, Xiaoxu, Peng, Chao, Gong, Fan, Chen, Yong. Crystal Structure of MLL2 Complex Guides the Identification of a Methylation Site on P53 Catalyzed by KMT2 Family Methyltransferases. STRUCTURE[J]. 2020, 28(10): 1141-+, http://dx.doi.org/10.1016/j.str.2020.07.002.
[12] Chen, Xia, Dong, Yao, Guo, Tianyue, Yang, ChaoYie, Chen, Yong, Sun, Haiying. N-terminal modified cyclopeptidic mimetics of Apollo(TBM) as inhibitors of TRF2. BIOORGANIC & MEDICINAL CHEMISTRY LETTERS[J]. 2020, 30(21): http://dx.doi.org/10.1016/j.bmcl.2020.127401.
[13] Alessandro Cicconi, Rekha Rai, Xuexue Xiong, Cayla Broton, Amer AlHiyasat, Chunyi Hu, Siying Dong, Wenqi Sun, Jennifer Garbarino, Ranjit S Bindra, Carl Schildkraut, Yong Chen, Sandy Chang. Microcephalin 1/BRIT1-TRF2 interaction promotes telomere replication and repair, linking telomere dysfunction to primary microcephaly. NATURE COMMUNICATIONS[J]. 2020, 11(1): https://doaj.org/article/1ffe736cceae4b6a9ede6a1d1309b5e3.
[14] Guochao Chen, Duo Wang, Bin Wu, Fuxiang Yan, Hongjuan Xue, Quanmeng Wang, Shu Quan, Yong Chen. Taf14 recognizes a common motif in transcriptional machineries and facilitates their clustering by phase separation. NATURE COMMUNICATIONS[J]. 2020, 11(1): http://dx.doi.org/10.1038/s41467-020-18021-7.
[15] Chen, Yong. The structural biology of the shelterin complex. BIOLOGICAL CHEMISTRYnull. 2019, 400(4): 457-466, http://dx.doi.org/10.1515/hsz-2018-0368.
[16] Xue, Han, Yao, Tonghui, Cao, Mi, Zhu, Guanjun, Li, Yan, Yuan, Guiyong, Chen, Yong, Lei, Ming, Huang, Jing. Structural basis of nucleosome recognition and modification by MLL methyltransferases. NATURE[J]. 2019, 573(7774): 445-+, https://www.webofscience.com/wos/woscc/full-record/WOS:000486647800056.
[17] Bazopoulou, Daphne, Knoefler, Daniela, Zheng, Yongxin, Ulrich, Kathrin, Oleson, Bryndon J, Xie, Lihan, Kim, Minwook, Kaufmann, Anke, Lee, YoungTae, Dou, Yali, Chen, Yong, Quan, Shu, Jakob, Ursula. Developmental ROS individualizes organismal stress resistance and lifespan. NATURE[J]. 2019, 576(7786): 301-+, [18] Hu, Chunyi, Inoue, Haruna, Sun, Wenqi, Takeshita, Yumiko, Huang, Yaoguang, Xu, Ying, Kanoh, Junko, Chen, Yong. The Inner Nuclear Membrane Protein Bqt4 in Fission Yeast Contains a DNA-Binding Domain Essential for Telomere Association with the Nuclear Envelope. STRUCTURE[J]. 2019, 27(2): 335-+, http://dx.doi.org/10.1016/j.str.2018.10.010.
[19] Han, Jianming, Li, Tingting, Li, Yanjing, Li, Muchun, Wang, Xiaoman, Peng, Chao, Su, Chen, Li, Na, Li, Yiwen, Xu, Ying, Chen, Yong. The internal interaction in RBBP5 regulates assembly and activity of MLL1 methyltransferase complex. NUCLEIC ACIDS RESEARCH[J]. 2019, 47(19): 10426-10438, [20] Hu, Chunyi, Inoue, Haruna, Sun, Wenqi, Takeshita, Yumiko, Huang, Yaoguang, Xu, Ying, Kanoh, Junko, Chen, Yong. Structural insights into chromosome attachment to the nuclear envelope by an inner nuclear membrane protein Bqt4 in fission yeast. NUCLEIC ACIDS RESEARCH[J]. 2019, 47(3): 1573-1584, [21] Li, Qing, Li, Yanjing, Yang, Suming, Huang, Shuo, Yan, Meng, Ding, Yifu, Tang, Wei, Lou, Xiwen, Yin, Qi, Sun, Zhanfei, Lu, Lei, Shi, Huijuan, Wang, Hongyan, Chen, Yong, Li, Jinsong. CRISPR-Cas9-mediated base-editing screening in mice identifies DND1 amino acids that are critical for primordial germ cell development. NATURE CELL BIOLOGY[J]. 2018, 20(11): 1315-+, http://202.127.25.143/handle/331003/3387.
[22] Chen Yong. Structural and functional analysis of the mammalian TIN2-TPP1-TRF2 telomeric complex. Cell Research. 2017, [23] Wang, Xiaoman, Zhao, Yiyue, Zhang, Jian, Chen, Yong. Structural basis for DAXX interaction with ATRX. PROTEIN & CELL[J]. 2017, 8(10): 767-771, http://lib.cqvip.com/Qikan/Article/Detail?id=673698844.
[24] Rai, Rekha, Hu, Chunyi, Broton, Cayla, Chen, Yong, Lei, Ming, Chang, Sandy. NBS1 Phosphorylation Status Dictates Repair Choice of Dysfunctional Telomeres. MOLECULAR CELL[J]. 2017, 65(5): 801-+, http://dx.doi.org/10.1016/j.molcel.2017.01.016.
[25] Karatas, Hacer, Li, Yangbing, Liu, Liu, Ji, Jiao, Lee, Shirley, Chen, Yong, Yang, Jiuling, Huang, Liyue, Bernard, Denzil, Xu, Jing, Townsend, Elizabeth C, Cao, Fang, Ran, Xu, Li, Xiaoqin, Wen, Bo, Sun, Duxin, Stuckey, Jeanne A, Lei, Ming, Dou, Yali, Wang, Shaomeng. Discovery of a Highly Potent, Cell-Permeable Macrocyclic Peptidomimetic (MM-589) Targeting the WD Repeat Domain 5 Protein (WDR5)-Mixed Lineage Leukemia (MLL) Protein-Protein Interaction. JOURNAL OF MEDICINAL CHEMISTRY[J]. 2017, 60(12): 4818-4839, https://www.webofscience.com/wos/woscc/full-record/WOS:000404202200004.
[26] Chen Yong. Structure of the fission yeast S. pombe telomeric Poz1-Tpz1-Rap1 complex. Cell Research. 2017, [27] Rekha Rai, Yong Chen, Ming Lei, Sandy Chang. TRF2-RAP1 is required to protect telomeres from engaging in homologous recombination-mediated deletions and fusions. NATURE COMMUNICATIONS[J]. 2016, 7(1): https://doaj.org/article/8b76e8537c054f68b658ac80288e929e.
[28] Ran, Xu, Liu, Liu, Yang, ChaoYie, Lu, Jianfeng, Chen, Yong, Lei, Ming, Wang, Shaomeng. Design of High-Affinity Stapled Peptides To Target the Repressor Activator Protein 1 (RAP1)/Telomeric Repeat-Binding Factor 2 (TRF2) Protein-Protein Interaction in the Shelterin Complex. JOURNAL OF MEDICINAL CHEMISTRY[J]. 2016, 59(1): 328-334, [29] Li, Yanjing, Han, Jianming, Zhang, Yuebin, Cao, Fang, Liu, Zhijun, Li, Shuai, Wu, Jian, Hu, Chunyi, Wang, Yan, Shuai, Jin, Chen, Juan, Cao, Liaoran, Li, Dangsheng, Shi, Pan, Tian, Changlin, Zhang, Jian, Dou, Yali, Li, Guohui, Chen, Yong, Lei, Ming. Structural basis for activity regulation of MLL family methyltransferases. NATURE[J]. 2016, 530(7591): 447-+, http://dx.doi.org/10.1038/nature16952.
[30] Yin, Jinhu, Wan, Bingbing, Sarkar, Jaya, Horvath, Kent, Wu, Jian, Chen, Yong, Cheng, Guangjuan, Wan, Ke, Chin, Peiju, Lei, Ming, Liu, Yie. Dimerization of SLX4 contributes to functioning of the SLX4-nuclease complex. NUCLEIC ACIDS RESEARCH[J]. 2016, 44(10): 4871-4880, http://dx.doi.org/10.1093/nar/gkw354.
[31] Yang, Yul W, Flynn, Ryan A, Chen, Yong, Qu, Kun, Wan, Bingbing, Wang, Kevin C, Lei, Ming, Chang, Howard Y. Essential role of lncRNA binding for WDR5 maintenance of active chromatin and embryonic stem cell pluripotency. ELIFE[J]. 2014, 3: https://doaj.org/article/637b963f6a21489bb28c31e39daf46c0.
[32] Chen, Yong, Cao, Fang, Wan, Bingbing, Dou, Yali, Lei, Ming. Structure of the SPRY domain of human Ash2L and its interactions with RbBP5 and DPY30. CELL RESEARCH[J]. 2012, 22(3): 598-602, http://lib.cqvip.com/Qikan/Article/Detail?id=41272769.
[33] Chen, Yong, Wan, Bingbing, Wang, Kevin C, Cao, Fang, Yang, Yuting, Protacio, Angeline, Dou, Yali, Chang, Howard Y, Lei, Ming. Crystal structure of the N-terminal region of human Ash2L shows a winged-helix motif involved in DNA binding. EMBO REPORTS[J]. 2011, 12(8): 797-803, https://www.webofscience.com/wos/woscc/full-record/WOS:000293326500014.
[34] Yong Chen, Rekha Rai, ZiRen Zhou, Junko Kanoh, Cyril Ribeyre, Yuting Yang, Hong Zheng, Pascal Damay, Feng Wang, Hisayo Tsujii, Yasushi Hiraoka, David Shore, HongYu Hu, Sandy Chang, Ming Lei. A Conserved Motif within RAP1 Plays Diversified Roles in Telomere Protection and Regulation in Different Organisms. NATURE STRUCTURAL & MOLECULAR BIOLOGY. 2011, 18(2): 213-221, https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3688267/.
[35] Cao, Fang, Chen, Yong, Cierpicki, Tomasz, Liu, Yifan, Basrur, Venkatesha, Lei, Ming, Dou, Yali. An Ash2L/RbBP5 Heterodimer Stimulates the MLL1 Methyltransferase Activity through Coordinated Substrate Interactions with the MLL1 SET Domain. PLOS ONE[J]. 2010, 5(11): https://doaj.org/article/a2086b79277d4977bd14aefd94468dbf.
[36] Chen Yong. Structural basis of selective ubiquitination of TRF1 bys SCFFBX4. Dev Cell. 2010, [37] Jiang, Jiansen, Zhang, Xuefeng, Chen, Yong, Wu, Yi, Zhou, Z Hong, Chang, Zengyi, Sui, SenFang. Activation of DegP chaperone-protease via formation of large cage-like oligomers upon binding to substrate proteins. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA[J]. 2008, 105(33): 11939-11944, https://www.webofscience.com/wos/woscc/full-record/WOS:000258723800064.
[38] Chen, Yong, Pan, Xijiang, Tang, Ying, Quan, Shu, Tai, Phang C, Sui, SenFang. Full-length Escherichia coli SecA Dimerizes in a Closed Conformation in Solution as Determined by Cryo-electron Microscopy. JOURNAL OF BIOLOGICAL CHEMISTRY[J]. 2008, 283(43): 28783-28787, http://dx.doi.org/10.1074/jbc.C800160200.
[39] Chen, Yong, Yang, Yuting, van Overbeek, Megan, Donigian, Jill R, Baciu, Paul, de Lange, Titia, Lei, Ming. A shared docking motif in TRF1 and TRF2 used for differential recruitment of telomeric proteins. SCIENCE[J]. 2008, 319(5866): 1092-1096, https://www.webofscience.com/wos/woscc/full-record/WOS:000253311700044.
[40] Chen, Yong, Tai, Phang C, Sui, SenFang. The active ring-like structure of SecA revealed by electron crystallography: Conformational change upon interaction with SecB. JOURNAL OF STRUCTURAL BIOLOGY[J]. 2007, 159(1): 149-153, http://dx.doi.org/10.1016/j.jsb.2007.01.015.
[41] Chen, Yong, Yang, Yuting, Wang, Feng, Wan, Ke, Yamane, Kenichi, Zhang, Yi, Lei, Ming. Crystal structure of human histone lysine-specific demethylase 1 (LSD1). PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA[J]. 2006, 103(38): 13956-13961, https://www.webofscience.com/wos/woscc/full-record/WOS:000240746600012.
[42] 王世真. Escherichia Coli Cora Periplasmic Domain Functions As A Homotetramer To Bind Substrate. JOURNAL OF BIOLOGICAL CHEMISTRY[J]. 2006, 281(37): 26813-26820, http://dx.doi.org/10.1074/jbc.M602342200.
[43] Liu, CZ, Chen, Y, Sui, SF. The identification of a new actin-binding region in p57. CELL RESEARCH[J]. 2006, 16(1): 106-112, https://www.webofscience.com/wos/woscc/full-record/WOS:000236961100014.
[44] Chen, Y, Song, JM, Sui, SF, Wang, DN. DnaK and DnaJ facilitated the folding process and reduced inclusion body formation of magnesium transporter CorA overexpressed in Escherichia coli. PROTEIN EXPRESSION AND PURIFICATION[J]. 2003, 32(2): 221-231, http://dx.doi.org/10.1016/S1046-5928(03)00233-X.
[45] 隋森芳. Ring-like pore structures of SecA: implication for bacterial protein-conducting channels. PROC NATL ACAD SCI USA. 2003, 100: 4221–4226-, [46] Chen, Y, Lu, YJ, Wang, HW, Quan, S, Chang, ZY, Sui, SF. Two-dimensional crystallization of a small heat shock protein HSP16.3 on lipid layer. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS[J]. 2003, 310(2): 360-366, https://www.webofscience.com/wos/woscc/full-record/WOS:000185904300017.
[47] Lijie Zhao, Naizhe Huang, Jun Mencius, Yanjing Li, Ying Xu, Yongxin Zheng, Wei He, Na Li, Jun Zheng, Min Zhuang, Shu Quan, Yong Chen. DPY30 acts as an ASH2L-specific stabilizer to stimulate the enzyme activity of MLL family methyltransferases on different substrates. ISCIENCE. http://dx.doi.org/10.1016/j.isci.2022.104948.

   

（ 1 ） 组蛋白甲基转移酶SET1家族蛋白活性调控的结构基础, 主持, 国家级, 2015-01--2018-12
（ 2 ） 蛋白质复合物调控染色体结构的分子机理, 主持, 国家级, 2013-01--2017-12
（ 3 ） 端粒酶复合体与染色质重塑复合物的结构与功能研究, 主持, 部委级, 2014-01--2018-12
（ 4 ） 非编码RNA和MLL复合物协同作用调控基因 转录的分子机制, 主持, 省级, 2014-09--2017-08
（ 5 ） SWI/SNF家族染色质重塑蛋白复合物的结构和功能研究, 主持, 国家级, 2017-01--2020-12
（ 6 ） SET1家族复合物在胚胎干细胞命运决定中发挥功能的分子机制, 主持, 国家级, 2020-01--2023-12

已指导学生

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