1.以人类疾病为导向，综合利用结构生物学、生物化学、化学生物学等手段的交叉学科研究，具体包括：研究与癌症、神经退行性疾病相关的细胞自噬（Autophagy）通路，揭示相关未知的分子机制和致病机理；研究与癌症等疾病相关的重要E3泛素酶和去泛素酶的结构与功能关系，并研发相关的小分子抑制剂，用于后续相关的化学生物学和潜在的疾病治疗研究。

2. 活性小分子的化学生物学研究，具体包括：天然活性小分子与靶点蛋白相互作用的机制研究；基于SAR-by-NMR方法，针对与癌症等疾病相关的重要生物靶点的小分子先导物的筛选和优化，及后期基于此先导物的机理和功能研究。

   

0703Z1-化学生物学
070303-有机化学
070302-分析化学

活性小分子与蛋白质相互作用的机制研究
蛋白质核磁共振分析
与疾病相关的蛋白质的结构与功能研究

2005-09--2010-06   香港科技大学   博士
2001-09--2005-07   复旦大学   本科

   

2013-01~2013-04,美国哈佛大学, 访问学者
2012-08~现在, 中国科学院上海有机化学研究所, 研究员
2011-03~2012-08,香港科技大学, 研究助理教授
2010-08~2011-02,香港科技大学, 助理研究员

2017-11-01-2021-10-31,中国生物物理学会生物磁共振专业委员会副秘书长, 副秘书长
2014-10-01-2016-10-31,《中国化学快报》青年编委会委员, 青年编委会委员

生物有机化学

   

（1） 上海市科技系统青年五四奖章, 市地级, 2018
（2） 中国化学会青年化学奖, 一等奖, 其他, 2017

   

[1] Gong, Xinyu, Pan, Lifeng. ATG16L1 is equipped with two distinct WIPI2-binding sites to drive autophagy. AUTOPHAGY. 2023, http://dx.doi.org/10.1080/15548627.2023.2213038.
[2] Tao Zhang, Daichao Xu, Jianping Liu, Min Wang, LiJuan Duan, Min Liu, Huyan Meng, Yuan Zhuang, Huibing Wang, Yingnan Wang, Mingming Lv, Zhengyi Zhang, Jia Hu, Linyu Shi, Rui Guo, Xingxing Xie, Hui Liu, Emily Erickson, Yaru Wang, Wenyu Yu, Fabin Dang, Dongxian Guan, Cong Jiang, Xiaoming Dai, Hiroyuki Inuzuka, Peiqiang Yan, Jingchao Wang, Mrigya Babuta, Gewei Lian, Zhenbo Tu, Ji Miao, Gyongyi Szabo, GuoHua Fong, Antoine E Karnoub, YuRu Lee, Lifeng Pan, William G Kaelin, Junying Yuan, Wenyi Wei. Prolonged hypoxia alleviates prolyl hydroxylation-mediated suppression of RIPK1 to promote necroptosis and inflammation. NATURE CELL BIOLOGY[J]. 2023, 25(7): 950-962, https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10617019/.
[3] Ding, Wenping, Zhou, Minqi, Li, Huayu, Li, Miao, Qiu, Yanping, Yin, Yu, Pan, Lifeng, Yang, Wenchao, Du, Yanan, Zhang, Xingang, Tang, Zhijun, Liu, Wen. Biocatalytic Fluoroalkylation Using Fluorinated S-Adenosyl-l-methionine Cofactors. ORGANIC LETTERS[J]. 2023, 25(30): 5650-5655, http://dx.doi.org/10.1021/acs.orglett.3c02028.
[4] Tao Zhang, Daichao Xu, Elijah Trefts, Mingming Lv, Hiroyuki Inuzuka, Guobin Song, Min Liu, Jianlin Lu, Jianping Liu, Chen Chu, Min Wang, Huibing Wang, Huyan Meng, Hui Liu, Yuan Zhuang, Xingxing Xie, Fabin Dang, Dongxian Guan, Yuqin Men, Shuwen Jiang, Cong Jiang, Xiaoming Dai, Jing Liu, Zhen Wang, Peiqiang Yan, Jingchao Wang, Zhenbo Tu, Mrigya Babuta, Emily Erickson, Alissandra L Hillis, Christian C Dibble, John M Asara, Gyongy Szabo, Piotr Sicinski, Ji Miao, YuRu Lee, Lifeng Pan, Reuben J Shaw, Junying Yuan, Wenyi Wei. Metabolic orchestration of cell death by AMPK-mediated phosphorylation of RIPK1. Science[J]. 2023, 380(6652): 1372-1380, https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10617018/.
[5] Xiaohui Qiu, Ying Li, Yingli Wang, Xinyu Gong, Yaru Wang, Lifeng Pan. Mechanistic Insights into the Interactions of Arl8b with the RUN Domains of PLEKHM1 and SKIP. JOURNAL OF MOLECULAR BIOLOGY[J]. 2023, 435(22): http://dx.doi.org/10.1016/j.jmb.2023.168293.
[6] Gong, Xinyu, Wang, Yingli, Tang, Yubin, Wang, Yaru, Zhang, Mingfang, Li, Miao, Zhang, Yuchao, Pan, Lifeng. ATG16L1 adopts a dual-binding site mode to interact with WIPI2b in autophagy. SCIENCE ADVANCES[J]. 2023, 9(9): http://dx.doi.org/10.1126/sciadv.adf0824.
[7] Xu, Xiaolong, Wang, Yaru, Zhang, Yan, Wang, Yingli, Yin, Yue, Peng, Chao, Gong, Xinyu, Li, Miao, Zhang, Yuchao, Zhang, Mingfang, Tang, Yubin, Zhou, Xindi, Liu, Haobo, Pan, Lifeng. Mechanistic insights into the enzymatic activity of E3 ligase HOIL-1L and its regulation by the linear ubiquitin chain binding. Science Advances[J]. 2023, 9(41): https://www.science.org/doi/full/10.1126/sciadv.adi4599?rfr_dat=cr_pub++0pubmed&url_ver=Z39.88-2003&rfr_id=ori%3Arid%3Acrossref.org.
[8] Yuchun Zhao, Xiangyang Liu, Zhihong Xiao, Jie Zhou, Xingyu Song, Xiaozheng Wang, Lijun Hu, Ying Wang, Peng Sun, Wenning Wang, Xinyi He, Shuangjun Lin, Zixin Deng, Lifeng Pan, Ming Jiang. O -methyltransferase-like enzyme catalyzed diazo installation in polyketide biosynthesis. NATURE COMMUNICATIONS[J]. 2023, 14(1): 1-14, http://dx.doi.org/10.1038/s41467-023-41062-7.
[9] Wang, Hongbo, Zou, Yike, Li, Miao, Tang, Zhijun, Wang, Jiabao, Tian, Zhenhua, Strassner, Nina, Yang, Qian, Zheng, Qingfei, Guo, Yujiao, Liu, Wen, Pan, Lifeng, Houk, K N. A cyclase that catalyses competing 2+2 and 4+2 cycloadditions. NATURE CHEMISTRY[J]. 2023, 15(2): 177-184, [10] Liu, Zaizhou, Chen, Kaige, Dai, Jun, Xu, Peng, Sun, Wei, Liu, Wanlin, Zhao, Zhixin, Bennett, Steven P, Li, Peifeng, Ma, Tiancheng, Lin, Yuqi, Kawakami, Akinori, Yu, Jing, Wang, Fei, Wang, Chunxi, Li, Miao, Chase, Peter, Hodder, Peter, Spicer, Timothy P, Scampavia, Louis, Cao, Chunyang, Pan, Lifeng, Dong, Jiajia, Chen, Yong, Yu, Biao, Guo, Min, Fang, Pengfei, Fisher, David E, Wang, Jing. A unique hyperdynamic dimer interface permits small molecule perturbation of the melanoma oncoprotein MITF for melanoma therapy. CELL RESEARCH[J]. 2023, 33(1): 55-70, [11] Feng, KeNa, Zhang, Yue, Zhang, Mingfang, Yang, YanLong, Liu, JiKai, Pan, Lifeng, Zeng, Ying. A flavin-monooxygenase catalyzing oxepinone formation and the complete biosynthesis of vibralactone. NATURE COMMUNICATIONS[J]. 2023, 14(1): http://dx.doi.org/10.1038/s41467-023-39108-x.
[12] Liu, Guizhen, Du, Yanan, Fu, Tao, Han, Ying, Pan, Lifeng, Kang, Jingwu. Profiling protein interactions by purification with capillary monolithic affinity column in combination with label-free quantitative proteomics. JOURNAL OF CHROMATOGRAPHY A[J]. 2022, 1676: http://dx.doi.org/10.1016/j.chroma.2022.463273.
[13] Jianping Liu, Yaru Wang, Danni Wang, Yingli Wang, Xiaolong Xu, Yuchao Zhang, Ying Li, Mingfang Zhang, Xinyu Gong, Yubin Tang, Liqiang Shen, Miao Li, Lifeng Pan. Mechanistic insights into the subversion of the linear ubiquitin chain assembly complex by the E3 ligase IpaH1.4 of Shigella flexneri. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA[J]. 2022, 119(12): [14] Daniel J Klionsky, Amal Kamal AbdelAziz, TieShan Tang. GUIDELINES FOR THE USE AND INTERPRETATION OF ASSAYS FOR MONITORING AUTOPHAGY (4TH EDITION). AUTOPHAGY[J]. 2021, 17(1): 1-382, http://dx.doi.org/10.1080/15548627.2020.1797280.
[15] Pischedda, Francesca, Cirnaru, Maria Daniela, Ponzoni, Luisa, Sandre, Michele, Biosa, Alice, Perez Carrion, Maria, Marin, Oriano, Morari, Michele, Pan, Lifeng, Greggio, Elisa, Bandopadhyay, Rina, Sala, Mariaelvina, Piccoli, Giovanni. LRRK2 G2019S kinase activity triggers neurotoxic NSF aggregation. BRAIN[J]. 2021, 144: 1509-1525, http://dx.doi.org/10.1093/brain/awab073.
[16] Meng, Huyan, Wu, Guowei, Zhao, Xinsuo, Wang, Anhui, Li, Dekang, Tong, Yilun, Jin, Taijie, Cao, Ye, Shan, Bing, Hu, Shichen, Li, Ying, Pan, Lifeng, Tian, Xiaoxu, Wu, Ping, Peng, Chao, Yuan, Junying, Li, Guohui, Tan, Li, Wang, Zhaoyin, Li, Ying. Discovery of a cooperative mode of inhibiting RIPK1 kinase. CELL DISCOVERY[J]. 2021, 7(1): [17] Zhou, Zixuan, Liu, Jianping, Fu, Tao, Wu, Ping, Peng, Chao, Gong, Xinyu, Wang, Yingli, Zhang, Mingfang, Li, Ying, Wang, Yaru, Xu, Xiaolong, Li, Miao, Pan, Lifeng. Phosphorylation regulates the binding of autophagy receptors to FIP200 Claw domain for selective autophagy initiation. NATURE COMMUNICATIONS[J]. 2021, 12(1): https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7946963/.
[18] Shan, Hengyue, Liu, Jianping, Shen, Jiali, Dai, Jialin, Xu, Gang, Lu, Kuankuan, Han, Chao, Wang, Yaru, Xu, Xiaolong, Tong, Yilun, Xiang, Huaijiang, Ai, Zhiyuan, Zhuang, Guanglei, Hu, Junhao, Zhang, Zheng, Li, Ying, Pan, Lifeng, Tan, Li. Development of potent and selective inhibitors targeting the papain-like protease of SARS-CoV-2. CELL CHEMICAL BIOLOGY[J]. 2021, 28(6): 855-+, http://dx.doi.org/10.1016/j.chembiol.2021.04.020.
[19] Zhang, Haijiao, Lin, Lin, Liu, Jianping, Pan, Lifeng, Lin, Zhijie, Zhang, Mingjie, Zhang, Jiong, Cao, Ying, Zhu, Jinwei, Zhang, Rongguang. Phase Separation of MAGI2-Mediated Complex Underlies Formation of Slit Diaphragm Complex in Glomerular Filtration Barrier. JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY[J]. 2021, 32(8): 1946-1960, [20] Fu, Tao, Zhang, Mingfang, Zhou, Zixuan, Wu, Ping, Peng, Chao, Wang, Yingli, Gong, Xinyu, Li, Ying, Wang, Yaru, Xu, Xiaolong, Li, Miao, Shen, Liqiang, Pan, Lifeng. Structural and biochemical advances on the recruitment of the autophagy-initiating ULK and TBK1 complexes by autophagy receptor NDP52. SCIENCE ADVANCES[J]. 2021, 7(33): [21] Xu, Xiaolong, Liu, Jianping, Wang, Yingli, Wang, Yaru, Gong, Xinyu, Pan, Lifeng. Mechanistic Insights into the Interactions of Ras Subfamily GTPases with the SPN Domain of Autism-associated SHANK3. CHINESE JOURNAL OF CHEMISTRY[J]. 2020, 38(12): 1635-1641, [22] Liu, Guizhen, Fu, Tao, Han, Ying, Hu, Shichen, Zhang, Xuepei, Zheng, Mengmeng, Hao, Piliang, Pan, Lifeng, Kang, Jingwu. Probing Protein-Protein Interactions with Label-Free Mass Spectrometry Quantification in Combination with Affinity Purification by Spin-Tip Affinity Columns. ANALYTICAL CHEMISTRY[J]. 2020, 92(5): 3913-3922, https://www.webofscience.com/wos/woscc/full-record/WOS:000518234700057.
[23] Li, Ying, Cheng, Xiaofang, Li, Miao, Wang, Yingli, Fu, Tao, Zhou, Zixuan, Wang, Yaru, Gong, Xinyu, Xu, Xiaolong, Liu, Jianping, Pan, Lifeng. Decoding three distinct states of the Syntaxin17 SNARE motif in mediating autophagosome-lysosome fusion. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA[J]. 2020, 117(35): 21391-21402, https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7474698/.
[24] Kangmin Hua, Xiangyang Liu, Yuchun Zhao, Yaojie Gao, Lifeng Pan, Haoran Zhang, Zixin Deng, Ming Jiang. Offloading Role of a Discrete Thioesterase in Type II Polyketide Biosynthesis. MBIO[J]. 2020, 11(5): http://dx.doi.org/10.1128/mBio.01334-20.
[25] Shichen Hu, Yujiao Guo, Yingli Wang, Ying Li, Tao Fu, Zixuan Zhou, Yaru Wang, Jianping Liu, Lifeng Pan. Structure of Myosin VI. NATURE COMMUNICATIONS. 2019, 10(1): http://dx.doi.org/10.1038/s41467-019-11481-6.
[26] Qingfei, Zheng, Yukang, Gong, Yujiao, Guo, Zhixiong, Zhao, Zhuhua, Wu, Zixuan, Zhou, Dandan, Chen, 潘李锋, 刘文. 吡咯吲哚霉素生源合成过程中催化反式十氢萘生成的黄素腺嘌呤二核苷酸依赖的4+2环化酶的结构透视. 科学新闻[J]. 2019, 134-134, http://lib.cqvip.com/Qikan/Article/Detail?id=75888869504849574850494951.
[27] Shichen Hu, Yujiao Guo, Yingli Wang, Ying Li, Tao Fu, Zixuan Zhou, Yaru Wang, Jianping Liu, Lifeng Pan. Structure of Myosin VI/Tom1 complex reveals a cargo recognition mode of Myosin VI for tethering. NATURE COMMUNICATIONS[J]. 2019, 10(1): 1-12, [28] Pan, Lifeng, Liu, Jianping, Li, Ying, Qin, ZH. Structural Basis of Autophagy Regulatory Proteins. AUTOPHAGY: BIOLOGY AND DISEASES: BASIC SCIENCE[J]. 2019, 1206: 285-324, [29] Carrion, Maria Perez, Pischedda, Francesca, Biosa, Alice, Russo, Isabella, Straniero, Letizia, Civiero, Laura, Guida, Marianna, Gloeckner, Christian J, Ticozzi, Nicola, Tiloca, Cinzia, Mariani, Claudio, Pezzoli, Gianni, Duga, Stefano, Pichler, Irene, Pan, Lifeng, Landers, John E, Greggio, Elisa, Hess, Michael W, Goldwurm, Stefano, Piccoli, Giovanni. The LRRK2 Variant E193K Prevents Mitochondrial Fission Upon MPP plus Treatment by Altering LRRK2 Binding to DRP1. FRONTIERS IN MOLECULAR NEUROSCIENCE[J]. 2018, 11: https://www.webofscience.com/wos/woscc/full-record/WOS:000426267500001.
[30] Jianping Liu, Lifeng Pan. Structural bases of the assembly, recognition and disassembly of linear ubiquitin chain. BBA - MOLECULAR CELL RESEARCH. 2018, 1865(10): 1410-1422, http://dx.doi.org/10.1016/j.bbamcr.2018.07.003.
[31] Zheng, Qingfei, Gong, Yukang, Guo, Yujiao, Zhao, Zhixiong, Wu, Zhuhua, Zhou, Zixuan, Chen, Dandan, Pan, Lifeng, Liu, Wen. Structural Insights into a Flavin-Dependent 4+2 Cyclase that Catalyzes trans-Decalin Formation in Pyrroindomycin Biosynthesis. CELL CHEMICAL BIOLOGY[J]. 2018, 25(6): 718-+, http://dx.doi.org/10.1016/j.chembiol.2018.03.007.
[32] Fu, Tao, Liu, Jianping, Wang, Yingli, Xie, Xingqiao, Hu, Shichen, Pan, Lifeng. Mechanistic insights into the interactions of NAP1 with the SKICH domains of NDP52 and TAX1BP1. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA[J]. 2018, 115(50): E11651-E11660, http://dx.doi.org/10.1073/pnas.1811421115.
[33] Xu, Daichao, Jin, Taijie, Zhu, Hong, Chen, Hongbo, Ofengeim, Dimitry, Zou, Chengyu, Mifflin, Lauren, Pan, Lifeng, Amin, Palak, Li, Wanjin, Shan, Bing, Naito, Masanori Gomi, Meng, Huyan, Li, Ying, Pan, Heling, Aron, Liviu, Adiconis, Xian, Levin, Joshua Z, Yankner, Bruce A, Yuan, Junying. TBK1 Suppresses RIPK1-Driven Apoptosis and Inflammation during Development and in Aging. CELL[J]. 2018, 174(6): 1477-+, http://dx.doi.org/10.1016/j.cell.2018.07.041.
[34] Li, Faxiang, Xu, Daichao, Wang, Yingli, Zhou, Zixuan, Liu, Jianping, Hu, Shichen, Gong, Yukang, Yuan, Junying, Pan, Lifeng. Structural insights into the ubiquitin recognition by OPTN (optineurin) and its regulation by TBK1-mediated phosphorylation. AUTOPHAGY[J]. 2018, 14(1): 66-79, https://www.webofscience.com/wos/woscc/full-record/WOS:000427049900006.
[35] Maria Perez Carrion, Francesca Pischedda, Alice Biosa, Isabella Russo, Letizia Straniero, Laura Civiero, Marianna Guida, Christian J Gloeckner, Nicola Ticozzi, Cinzia Tiloca, Claudio Mariani, Gianni Pezzoli, Stefano Duga, Irene Pichler, Lifeng Pan, John E Landers, Elisa Greggio, Michael W Hess, Stefano Goldwurm, Giovanni Piccoli. The LRRK2 Variant E193K Prevents Mitochondrial Fission Upon MPP+ Treatment by Altering LRRK2 Binding to DRP1. FRONTIERS IN MOLECULAR NEUROSCIENCE[J]. 2018, 11: https://doaj.org/article/966cd6a9f2404d1fad07e6bf77e844ea.
[36] Liu, Jianping, Pan, Lifeng. Structural bases of the assembly, recognition and disassembly of linear ubiquitin chain. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCHnull. 2018, 1865(10): 1410-1422, http://dx.doi.org/10.1016/j.bbamcr.2018.07.003.
[37] Hu, Shichen, Wang, Yingli, Gong, Yukang, Liu, Jianping, Li, Ying, Pan, Lifeng. Mechanistic Insights into Recognitions of Ubiquitin and Myosin VI by Autophagy Receptor TAX1BP1. JOURNAL OF MOLECULAR BIOLOGY[J]. 2018, 430(18): 3283-3296, http://dx.doi.org/10.1016/j.jmb.2018.06.030.
[38] Wei, Ran, Xu, Lily Wen, Liu, Jianping, Li, Yanxia, Zhang, Pei, Shan, Bing, Lu, Xiaojuan, Qian, Lihui, Wu, Zheming, Dong, Kangyun, Zhu, Hong, Pan, Lifeng, Yuan, Junying, Pan, Heling. SPATA2 regulates the activation of RIPK1 by modulating linear ubiquitination. GENES & DEVELOPMENT[J]. 2017, 31(11): 1162-1176, https://www.webofscience.com/wos/woscc/full-record/WOS:000405578600010.
[39] Zhang, Zhuan, Gong, YuKang, Zhou, Qiang, Hu, Yu, Ma, HongMin, Chen, YongSheng, Igarashi, Yasuhiro, Pan, Lifeng, Tang, GongLi. Hydroxyl regioisomerization of anthracycline catalyzed by a four-enzyme cascade. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA[J]. 2017, 114(7): 1554-1559, http://dx.doi.org/10.1073/pnas.1610097114.
[40] Liu, Jianping, Wang, Yingli, Gong, Yukang, Fu, Tao, Hu, Shichen, Zhou, Zixuan, Pan, Lifeng. Structural Insights into SHARPIN-Mediated Activation of HOIP for the Linear Ubiquitin Chain Assembly. CELL REPORTS[J]. 2017, 21(1): 27-36, https://doaj.org/article/8068bbaa82e6433b8c7d3e2e779f354b.
[41] Xu, Daichao, Liu, Jianping, Fu, Tao, Shan, Bing, Qian, Lihui, Pan, Lifeng, Yuan, Junying. USP25 regulates Wnt signaling by controlling the stability of tankyrases. GENES & DEVELOPMENT[J]. 2017, 31(10): 1024-1035, https://www.webofscience.com/wos/woscc/full-record/WOS:000403831200007.
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[43] Faxiang Li, Xingqiao Xie, Yingli Wang, Jianping Liu, Xiaofang Cheng, Yujiao Guo, Yukang Gong, Shichen Hu, Lifeng Pan. Structural insights into the interaction and disease mechanism of neurodegenerative disease-associated optineurin and TBK1 proteins. NATURE COMMUNICATIONS[J]. 2016, 7(1): https://doaj.org/article/6f149eadfad341e89e90455b3ba5ac86.
[44] Cheng, Xiaofang, Wang, Yingli, Gong, Yukang, Li, Faxiang, Guo, Yujiao, Hu, Shichen, Liu, Jianping, Pan, Lifeng. Structural basis of FYCO1 and MAP1LC3A interaction reveals a novel binding mode for Atg8-family proteins. AUTOPHAGY[J]. 2016, 12(8): 1330-1339, https://www.webofscience.com/wos/woscc/full-record/WOS:000382309200008.
[45] Xie, Xingqiao, Li, Faxiang, Wang, Yuanyuan, Wang, Yingli, Lin, Zhijie, Cheng, Xiaofang, Liu, Jianping, Chen, Changbin, Pan, Lifeng. Molecular basis of ubiquitin recognition by the autophagy receptor CALCOCO2. AUTOPHAGY[J]. 2015, 11(10): 1775-1789, https://www.webofscience.com/wos/woscc/full-record/WOS:000363867800006.
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[49] 张明杰. IL-1β and reactive oxygen species differentially regulate neutrophil directional migration and Basal random motility in a zebrafish injury-induced inflammation model. J IMMUNOL. 2014, [50] Piccoli, Giovanni, Onofri, Franco, Cirnaru, Maria Daniela, Kaiser, Christoph J O, Jagtap, Pravinkumar, Kastenmueller, Andreas, Pischedda, Francesca, Marte, Antonella, von Zweydorf, Felix, Vogt, Andreas, Giesert, Florian, Pan, Lifeng, Antonucci, Flavia, Kiel, Christina, Zhang, Mingjie, Weinkauf, Sevil, Sattler, Michael, Sala, Carlo, Matteoli, Michela, Ueffing, Marius, Gloeckner, Christian Johannes. Leucine-Rich Repeat Kinase 2 Binds to Neuronal Vesicles through Protein Interactions Mediated by Its C-Terminal WD40 Domain. MOLECULAR AND CELLULAR BIOLOGY[J]. 2014, 34(12): 2147-2161, https://www.webofscience.com/wos/woscc/full-record/WOS:000336743200004.
[51] VAKIFAHMETOGLUNORBERG HELIN, KIM MINSU, XIA HONGGUANG, IWANICKI MARCIN P, OFENGEIM DIMITRY, COLOFF JONATHAN L, Pan Lifeng, INCE TAN A, KROEMER GUIDO, BRUGGE JOAN S, Yuan Junying. Chaperone-mediated autophagy degrades mutant p53. GENES DEV.[J]. 2013, 27(15): 1718-1730, http://www.irgrid.ac.cn/handle/1471x/870641.
[52] VakifahmetogluNorberg, Helin, Kim, Minsu, Xia, Hongguang, Iwanicki, Marcin P, Ofengeim, Dimitry, Coloff, Jonathan L, Pan, Lifeng, Ince, Tan A, Kroemer, Guido, Brugge, Joan S, Yuan, Junying. Chaperone-mediated autophagy degrades mutant p53. GENES & DEVELOPMENT[J]. 2013, 27(15): 1718-1730, http://dx.doi.org/10.1101/gad.220897.113.
[53] 张明杰. Membrane-induced lever arm expansion allows myosin VI to walk with large and variable step sizes. J BIOL CHEMAUGUST30. 2012, [54] Pan, Lifeng, Zhang, Mingjie. Structures of Usher Syndrome 1 Proteins and Their Complexes. PHYSIOLOGYnull. 2012, 27(1): 25-42, https://www.webofscience.com/wos/woscc/full-record/WOS:000300408000004.
[55] Li, Xiaohua, He, Liqiang, Che, Ka Hing, Funderburk, Sarah F, Pan, Lifeng, Pan, Nina, Zhang, Mingjie, Yue, Zhenyu, Zhao, Yanxiang. Imperfect interface of Beclin1 coiled-coil domain regulates homodimer and heterodimer formation with Atg14L and UVRAG. NATURE COMMUNICATIONS[J]. 2012, 3: 662-, https://www.webofscience.com/wos/woscc/full-record/WOS:000302060100003.
[56] 张明杰. Cargo Recognition Mechanism of Myosin X Revealed by the Structure of its Tail MyTH4-FERM Tandem in Complex with the DCC P3 Domain. PNAS. 2011, [57] 张明杰. The Structure of the PDZ3-SH3-GuK Tandem of ZO-1 Suggests a Supramodular Organization of the MAGUK Family Scaffold Protein Core. J BIOL CHEM. 2011, [58] FENG Wei, PAN LiFeng, ZHANG MingJie. Combination of NMR spectroscopy and X-ray crystallography offers unique advantages for elucidation of the structural basis of protein complex assembly. 中国科学：生命科学英文版[J]. 2011, 54(2): 101-111, http://lib.cqvip.com/Qikan/Article/Detail?id=36779055.
[59] 张明杰. Structure of MyTH4-FERM Domains in Myosin VIIa Tail Bound to Cargo. SCIENCE[J]. 2011, 331(6018): 757-760, https://www.webofscience.com/wos/woscc/full-record/WOS:000287205700067.
[60] Feng Wei, Pan LiFeng, Zhang MingJie. Combination of NMR spectroscopy and X-ray crystallography offers unique advantages for elucidation of the structural basis of protein complex assembly. SCIENCE CHINA-LIFE SCIENCES[J]. 2011, 54(2): 101-111, http://www.irgrid.ac.cn/handle/1471x/757425.
[61] 张明杰. quot Redox-regulated lipid membrane binding of the PICK1 PDZ domain of the two Usher syndrome proteins. BIOCHEMISTRY. 2010, [62] 张明杰. The Structure of the harmonin/sans complex reveals an unexpected interaction mode of the two Usher syndrome proteins. PNAS. 2010, [63] Wang, Conan K, Pan, Lifeng, Chen, Jia, Zhang, Mingjie. Extensions of PDZ domains as important structural and functional elements. PROTEIN & CELLnull. 2010, 1(8): 737-751, http://lib.cqvip.com/Qikan/Article/Detail?id=7105124529.
[64] Wen, Wenyu, Yu, Jiang, Pan, Lifeng, Wei, Zhiyi, Weng, Jingwei, Wang, Wenning, Ong, Yan Shan, Tran, Ton Hoai Thi, Hong, Wanjin, Zhang, Mingjie. Lipid-Induced Conformational Switch Controls Fusion Activity of Longin Domain SNARE Ykt6. MOLECULAR CELL[J]. 2010, 37(3): 383-395, http://dx.doi.org/10.1016/j.molcel.2010.01.024.
[65] 韩家淮. Oxidation-induced intramolecular disulfide bond inactivates mitogen-activated protein kinase kinase 6 by inhibiting ATP binding. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA[J]. 2010, 107(49): 20974-20979, https://www.webofscience.com/wos/woscc/full-record/WOS:000285050800026.
[66] 张明杰. Assembling Stable Hair-Cell Tip Link Complex via Multi-dentate Interactions between Harmonin and Cadherin23. PNAS. 2009, [67] 张明杰. Domain-swapped dimerization of ZO-1 PDZ2 generates specific and regulatory connexin43-binding sites. EMBO J. 2008, [68] 张明杰. Clustering and synaptic targeting of PICK1 requires direct interaction between the PDZ domain and lipid membranes. EMBO J. 2007, [69] Xia Hong-guang, Najafov Ayaz, Geng Jiefei, Galan-Acosta Lorena, Han Xuemei, Guo Yuan, Shan Bing, Zhang Yaoyang, Norberg Erik, Zhang Tao, Pan Lifeng, Liu Junli, Coloff Jonathan L., Ofengeim Dimitry, Zhu Hong, Wu Kejia, Cai Yu, Yates John R., Zhu Zhengjiang, Yuan Junying, Vakifahmetoglu-Norberg Helin. Degradation of HK2 by chaperone-mediated autophagy promotes metabolic catastrophe and cell death. THE JOURNAL OF CELL BIOLOGY. 210(5): 705-716, https://www.webofscience.com/wos/woscc/full-record/WOS:000360685600006.
[70] Wu Lin, Pan Lifeng, Zhang Chuchu, Zhang Mingjie. Large Protein Assemblies Formed by Multivalent Interactions between Cadherin23 and Harmonin Suggest a Stable Anchorage Structure at the Tip Link of Stereocilia *. THE JOURNAL OF BIOLOGICAL CHEMISTRY. 287(40): 33460-33471, http://dx.doi.org/10.1074/jbc.M112.378505.

   

（ 1 ） 调控生命过程的若干天然产物的合成、靶点和机制研究, 参与, 国家级, 2013-01--2017-12
（ 2 ） 中组部国家****项目, 主持, 国家级, 2013-01--2015-12
（ 3 ） 细胞坏死小分子抑制剂Nec29的作用机制及相关的化学生物学研究, 主持, 省级, 2013-07--2015-06
（ 4 ） 国家自然科学基金面上项目, 主持, 国家级, 2015-01--2018-12
（ 5 ） 合成科学与生物创制专题, 参与, 省级, 2015-07--2018-06
（ 6 ） 天然产物的化学生物学, 参与, 国家级, 2017-01--2022-12
（ 7 ） 神经退行性疾病中细胞死亡机理和干预的研究, 参与, 国家级, 2016-06--2021-05
（ 8 ） 几个新颖[4+2]环加成酶的结构及其催化机制研究, 主持, 国家级, 2017-01--2020-12
（ 9 ） 天然产物生源合成过程中催化环化反应的几个新颖蛋白酶的结构、催化机制和改良应用研究, 主持, 省级, 2017-09--2020-08
（ 10 ） 蛋白质线性泛素化修饰的分子机制和小分子调控研究, 主持, 国家级, 2018-01--2020-12
（ 11 ） 蛋白-蛋白相互作用和蛋白-小分子相互作用的机制研究, 主持, 国家级, 2019-01--2021-12

已指导学生

张帅琰  硕士研究生  070303-有机化学  

李发祥  博士研究生  071009-细胞生物学  

程小芳  博士研究生  071009-细胞生物学  

郭玉娇  硕士研究生  085238-生物工程  

龚余康  博士研究生  0703Z1-化学生物学  

现指导学生

胡世尘  博士研究生  0703Z1-化学生物学  

龚新宇  硕士研究生  070303-有机化学  

李淼  硕士研究生  070303-有机化学  

付涛  博士研究生  0703Z1-化学生物学  

周子璇  博士研究生  0703Z1-化学生物学  

许晓龙  博士研究生  070303-有机化学  

王亚茹  博士研究生  0703Z1-化学生物学  

李颖  博士研究生  0703Z1-化学生物学  

张明芳  硕士研究生  070303-有机化学