1.       微生物细胞工厂的代谢调控

2.       重要医药中间体/生物活性物质的微生物合成生物技术

3.       应用环境生物技术

   

081703-生物化工
085238-生物工程

微生物代谢调控
2. 重要医药中间体生物催化与转化合成
应用环境微生物

2005-09--2008-07   中科院生态环境研究中心   博士
2002-07--2005-09   天津科技大学/中科院   硕士

博士研究生

博士学位

   

2011-09~现在, 中科院上海高等研究院, 副研究员
2008-12~2011-08,中科院上海生命科学研究院, 博士后

2018-06-01-今,Applied Biochemistry and Biotechnology审稿专家,

   

（1） SA-SIBS优秀青年人才奖励基金, 一等奖, 研究所（学校）, 2010

[1] 张保国, 刘相岑, 张静娴, 韩苏皖, 杜桂林, 史吉平. 一种用于生产制备9-OHAD 的基因工程菌株、及其制备方法和应用. 202310039555.6, 2023-01-27.
[2] 张保国, 刘相岑, 袁辰阳, 张静娴, 杜桂林, 韩苏皖, 史吉平. 一种产双降醇的基因工程菌及其用途. 2022107293262, 2022-06-24.
[3] 张保国, 袁辰阳, 刘相岑, 张静娴, 杜桂林, 韩苏皖, 史吉平. 一种产甾药前体的基因工程菌及其用途. 202210730036X, 2022-06-24.
[4] 张保国, 杜桂林, 史吉平, 袁辰阳, 刘向岑, 张静娴, 韩苏皖. 一种反刍动物用的棉籽壳发酵饲料及其制备方法. 2022105855710, 2022-05-26.
[5] 张保国, 杜桂林, 马治国, 刘相岑, 袁辰阳, 史吉平. 一种畜禽用的尾菜发酵饲料及其制备方法. CN: CN112369502A, 2021-02-19.
[6] 张保国, 刘相岑, 袁辰阳, 曹慧锦, 杜桂林, 史吉平. 一种微生物降解植物甾醇生产雄二烯二酮的方法. CN: CN111808830A, 2020-10-23.
[7] 张保国, 张瑞婕, 刘相岑, 韩煜畅, 史吉平, 姜标. 一种3-甾酮-△ 1 -脱氢酶及其编码基因和应用. 中国: CN108456666A, 2018-08-28.
[8] 张保国, 魏长龙, 曾俊华, 王珊, 史吉平, 姜标. 利用微生物转化制备9α-羟基-雄烯二酮的方法. CN: CN103361394A, 2013-10-23.

   

[1] Yuan, Chenyang, Ma, Zhiguo, Li, Yixin, Zhang, Jingxian, Liu, Xiangcen, Han, Suwan, Du, Guilin, Shi, Jiping, Sun, Junsong, Zhang, Baoguo. Production of 21-hydroxy-20-methyl-pregna-1,4-dien-3-one by modifying multiple genes in Mycolicibacterium. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY[J]. 2023, 107(5-6): 1563-1574, http://dx.doi.org/10.1007/s00253-023-12399-2.
[2] Xiangcen Liu, Jingxian Zhang, Chenyang Yuan, Guilin Du, Suwan Han, Jiping Shi, Junsong Sun, Baoguo Zhang. Improving the production of 9α-hydroxy-4-androstene-3,17-dione from phytosterols by 3-ketosteroid-Δ 1 -dehydrogenase deletions and multiple genetic modifications in Mycobacterium fortuitum. MICROBIAL CELL FACTORIES[J]. 2023, 22: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10018825/.
[3] Jingxian Zhang, Ruijie Zhang, Zhengding Su, Jiping Shi, Huijin Cao, Baoguo Zhang. Whole-Genome Analysis of Mycobacterium neoaurum DSM 1381 and the Validation of Two Key Enzymes Affecting C22 Steroid Intermediates in Sterol Metabolism. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES[J]. 2023, 24: https://doaj.org/article/aee74c75dffa4dcaa266fa8f9f84b722.
[4] Duan, Haiyan, Fu, Cong, Du, Guilin, Xie, Shiqiu, Liu, Min, Zhang, Baoguo, Shi, Jiping, Sun, Junsong. Dynamic Microstructure Assembly Driven by Lysinibacillus sp. LF-N1 and Penicillium oxalicum DH-1 Inoculants Corresponds to Composting Performance. MICROORGANISMS[J]. 2022, 10(4): [5] Zhang, Jingxian, Shi, Jiping, Yuan, Chenyang, Liu, Xiangcen, Du, Guilin, Fan, Ruimei, Zhang, Baoguo. MicroRNA Expression Profile Analysis of Chlamydomonas reinhardtii during Lipid Accumulation Process under Nitrogen Deprivation Stresses. BIOENGINEERING-BASEL[J]. 2022, 9(1): http://dx.doi.org/10.3390/bioengineering9010006.
[6] 张静娴, 杜桂林, 史吉平, 马治国, 袁辰阳, 刘相岑, 张保国. 甘蓝尾菜与干水稻秸秆混贮的发酵品质、菌群演替及关键菌群研究. 西北农林科技大学学报:自然科学版[J]. 2022, 50(2): 90-102, http://lib.cqvip.com/Qikan/Article/Detail?id=7106596629.
[7] Yuan, Chenyang, Song, Shikui, He, Jianxin, Zhang, Jingxian, Liu, Xiangcen, Pena, Eliana L, Sun, Junsong, Shi, Jiping, Su, Zhengding, Zhang, Baoguo. Bioconversion of Phytosterols to 9-Hydroxy-3-Oxo-4,17-Pregadiene-20-Carboxylic Acid Methyl Ester by Enoyl-CoA Deficiency and Modifying Multiple Genes in Mycolicibacterium neoaurum. APPLIED AND ENVIRONMENTAL MICROBIOLOGY[J]. 2022, 88(22): [8] Xu Zhang, YuMei Huang, HuaLi Qin, Zhang Baoguo, K P Rakesh, Haolin Tang. Copper-Promoted Conjugate Addition of Carboxylic Acids to Ethenesulfonyl Fluoride (ESF) for Constructing Aliphatic Sulfonyl Fluorides. ACS OMEGA[J]. 2021, 6(40): 25972-25981, http://dx.doi.org/10.1021/acsomega.1c02804.
[9] Jingxian Zhang, Jiping Shi, Chenyang Yuan, Xiangcen Liu, Guilin Du, Ruimei Fan, Baoguo Zhang, Giorgos Markou, ZhiYong Li. MicroRNA Expression Profile Analysis of Chlamydomonas reinhardtii during Lipid Accumulation Process under Nitrogen Deprivation Stresses. BIOENGINEERING[J]. 2021, 9(1): [10] Du, Guilin, Shi, Jiping, Zhang, Jingxian, Ma, Zhiguo, Liu, Xiangcen, Yuan, Chenyang, Zhang, Baoguo, Zhang, Zhanying, Harrison, Mark D. Exogenous Probiotics Improve Fermentation Quality, Microflora Phenotypes, and Trophic Modes of Fermented Vegetable Waste for Animal Feed. MICROORGANISMS[J]. 2021, 9(3): https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8003719/.
[11] 张保国. Exogenous Enzymes Amendment Accelerates Maturity and Changes Microflora Succession in Horse and Wildlife Animal Manures Co-composting. Environmental Science and Pollution Research[J]. 2021, [12] Du, Guilin, Zhang, Guilong, Shi, Jiping, Zhang, Jingxian, Ma, Zhiguo, Liu, Xiangcen, Yuan, Chenyang, Li, Xiang, Zhang, Baoguo. Keystone Taxa Lactiplantibacillus and Lacticaseibacillus Directly Improve the Ensiling Performance and Microflora Profile in Co-Ensiling Cabbage Byproduct and Rice Straw. MICROORGANISMS[J]. 2021, 9(5): [13] 马治国, 刘相岑, 袁辰阳, 杜桂林, 钮冰, 张保国, 史吉平. 转化植物甾醇产22-羟基-23，24-双降甾-1，4-二烯-3-酮（HPD）工程菌株的构建及发酵培养基优化. 食品工业科技[J]. 2021, 42(15): 131-138, http://lib.cqvip.com/Qikan/Article/Detail?id=7105250128.
[14] Du, Guilin, Feng, Wenwen, Cai, Hanbin, Ma, Zhiguo, Liu, Xiangcen, Yuan, Chenyang, Shi, Jiping, Zhang, Baoguo. Exogenous enzyme amendment accelerates maturity and changes microflora succession in horse and wildlife animal manure co-composting. ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH[J]. 2021, 28(17): 21610-21620, http://dx.doi.org/10.1007/s11356-020-11568-4.
[15] 张静娴, 杜桂林, 史吉平, 马治国, 袁辰阳, 刘相岑, 张保国. 甘蓝尾菜与干水稻秸秆混贮的发酵品质、菌群演替及关键菌群研究. 西北农林科技大学学报(自然科学版)[J]. 2021, 90-102, https://kns.cnki.net/kcms/detail/detail.aspx?dbcode=CJFD&dbname=CJFDAUTO&filename=XBNY202202011&v=MjgxNDZHNEhOUE1yWTlFWllSOGVYMUx1eFlTN0RoMVQzcVRyV00xRnJDVVI3aWZaT1puRmlEZ1ZyckJQUy9GZDc=.
[16] ChenYang Yuan, ZhiGuo Ma, JingXian Zhang, XiangCen Liu, GuiLin Du, JunSong Sun, JiPing Shi, BaoGuo Zhang. Production of 9,21-dihydroxy-20-methyl-pregna-4-en-3-one from phytosterols in Mycobacterium neoaurum by modifying multiple genes and improving the intracellular environment. MICROBIAL CELL FACTORIES[J]. 2021, 20: [17] Duan, Haiyan, Ji, Minghua, Chen, Ai, Zhang, Baoguo, Shi, Jiping, Liu, Li, Li, Xiang, Sun, Junsong. Evaluating the impact of rice husk on successions of bacterial and fungal communities during cow manure composting. ENVIRONMENTAL TECHNOLOGY & INNOVATION[J]. 2021, 24: http://dx.doi.org/10.1016/j.eti.2021.102084.
[18] 冯雯雯, 董永华, 蔡涵冰, 孙俊松, 张保国, 马中良. 微生物菌剂对畜禽粪便与秸秆混合发酵过程参数影响及腐熟度综合评价. 江苏农业科学[J]. 2020, 48(6): 265-271, https://kns.cnki.net/KCMS/detail/detail.aspx?dbcode=CJFQ&dbname=CJFDLAST2020&filename=JSNY202006050&v=MDQwMzBITXFZOUFaSVI4ZVgxTHV4WVM3RGgxVDNxVHJXTTFGckNVUjdxZVp1ZHZGeUhuV3I3QUx6N0ZkN0c0SE4=.
[19] 蔡涵冰, 冯雯雯, 董永华, 马中良, 曹慧锦, 孙俊松, 张保国. 畜禽粪便和桃树枝工业化堆肥过程中微生物群演替及其与环境因子的关系. 环境科学[J]. 2020, 997-1004, https://kns.cnki.net/KCMS/detail/detail.aspx?dbcode=CJFQ&dbname=CJFDLAST2020&filename=HJKZ202002059&v=MDY2NzJyWTlBYllSOGVYMUx1eFlTN0RoMVQzcVRyV00xRnJDVVI3cWVadWR2RnlIaFY3L0JMU2ZBZExHNEhOSE0=.
[20] Xiangcen Liu, Ruijie Zhang, Zhiwei Bao, Chenyang Yuan, Huijin Cao, Jiping Shi, Junsong Sun, Baoguo ZHANG. Biotransformation of phytosterols to ADD by Mycobacterium sp. ZFZ expressing 3-ketosteroid-Δ1-dehydrogenase. Catalysts[J]. 2020, [21] Liu, Xiangcen, Zhang, Ruijie, Bao, Zhiwei, Yuan, Chenyang, Cao, Huijin, Shi, Jiping, Sun, Junsong, Zhang, Baoguo. Biotransformation of Phytosterols to Androst-1,4-Diene-3,17-Dione byMycobacteriumsp. ZFZ Expressing 3-Ketosteroid-Delta(1)-Dehydrogenase. CATALYSTS[J]. 2020, 10(6): https://www.webofscience.com/wos/woscc/full-record/WOS:000554688300001.
[22] 曹慧锦, 马治国, 刘相岑, 蔡涵冰, 朱晨光, 张保国, 史吉平. 降解植物甾醇产9α羟基雄烯二酮工程菌株构建及发酵工艺优化. 食品工业科技[J]. 2020, 41(14): 101-107, https://kns.cnki.net/KCMS/detail/detail.aspx?dbcode=CJFQ&dbname=CJFDLAST2020&filename=SPKJ202014017&v=MDEzOTM0OUVZNFI4ZVgxTHV4WVM3RGgxVDNxVHJXTTFGckNVUjdxZVp1ZHZGeUhnVkwvUE5qM0FaTEc0SE5ITnE=.
[23] 孙稳舟, 张保国, 袁丽霞, 孙立洁, 史吉平, 姚建铭. 基于PB设计和BBD响应面法优化高山被孢霉产花生四烯酸发酵培养基. 安徽农业大学学报[J]. 2020, 47(1): 12-17, http://lib.cqvip.com/Qikan/Article/Detail?id=7101621106.
[24] Zhang, Ruijie, Xu, Xuexia, Cao, Huijin, Yuan, Chenyang, Yuminaga, Yuki, Zhao, Suwen, Shi, Jiping, Zhang, Baoguo. Purification, characterization, and application of a high activity 3-ketosteroid-Delta(1)-dehydrogenase from Mycobacterium neoaurum DSM 1381. APPLIED MICROBIOLOGY AND BIOTECHNOLOGY[J]. 2019, 103(16): 6605-6616, https://www.webofscience.com/wos/woscc/full-record/WOS:000477927800018.
[25] Yang, Chengyu, Fan, Weiming, Zhang, Ruijie, Shi, Jiping, KnezevicJugovic, Zorica, Zhang, Baoguo. Study on Transglucosylation Properties of Amylosucrase from Xanthomonas campestris pv. Campestris and Its Application in the Production of alpha-Arbutin. CATALYSTS[J]. 2019, 9(1): https://www.webofscience.com/wos/woscc/full-record/WOS:000459732000005.
[26] 刘相岑, 郝晓蔚, 张瑞婕, 冯雯雯, 朱晨光, 张保国, 史吉平. 降解植物甾醇产雄甾1，4-二烯-3，17-二酮工程菌株的构建及转化培养基优化. 食品工业科技[J]. 2018, 39(18): 110-116, http://lib.cqvip.com/Qikan/Article/Detail?id=676272778.
[27] Zhang, Ruijie, Liu, Xiangcen, Wang, Yushi, Han, Yuchang, Sun, Junsong, Shi, Jiping, Zhang, Baoguo. Identification, function, and application of 3-ketosteroid Delta l -dehydrogenase isozymes in Mycobacterium neoaurum DSM 1381 for the production of steroidic synthons. MICROBIAL CELL FACTORIES[J]. 2018, 17: https://www.webofscience.com/wos/woscc/full-record/WOS:000432820500001.
[28] 高山, 刘相岑, 朱晨光, 史吉平, 张保国. 尼罗红荧光光谱法快速测定解脂耶氏酵母油脂含量的研究. 食品工业科技[J]. 2017, 38(15): 270-274,323, http://www.corc.org.cn/handle/1471x/2185936.
[29] 柳相鹤, 张瑞婕, 赵树欣, 张保国, 史吉平. Mycobacterium sp. BFZ304转化植物甾醇产9α-羟基雄烯二酮培养基的响应面优化. 食品工业科技[J]. 2016, 37(16): 172-177, http://sciencechina.cn/gw.jsp?action=detail.jsp&internal_id=5843952&detailType=1.
[30] 霍欢欢, 柳相鹤, 史吉平, 刘志文, 张保国. 纤细裸藻Δ9-脂肪酸延长酶基因在解脂耶氏酵母中的表达. 食品工业科技[J]. 2015, 36(20): 183-187, http://sciencechina.cn/gw.jsp?action=detail.jsp&internal_id=5547672&detailType=1.
[31] 魏长龙, 赵树欣, 王珊, 史吉平, 张保国. Nocardia canicruria BF313催化甾体9 α-羟基化发酵工艺优化. 食品工业科技[J]. 2014, 35(18): 320-323,337, http://sciencechina.cn/gw.jsp?action=detail.jsp&internal_id=5228428&detailType=1.
[32] 赵权宇, 于水燕, 张保国, 史吉平. 小球藻联产油脂和虾青素的基元模式分析. 生物加工过程[J]. 2014, 12(4): 76-81, http://lib.cqvip.com/Qikan/Article/Detail?id=661653479.
[33] Zhang, Baoguo, Fan, Ruimei, Bai, Zhihui, Wang, Shan, Wang, Liang, Shi, Jiping. Biosorption characteristics of Bacillus gibsonii S-2 waste biomass for removal of lead (II) from aqueous solution. ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH[J]. 2013, 20(3): 1367-1373, http://ir.sari.ac.cn/handle/71783468/165.
[34] Li, Zuming, Bai, Zhihui, Zhang, Baoguo, Li, Baojv, Jin, Bo, Zhang, Michael, Lin, Francis, Zhang, Hongxun. Purification and Characterization of Alkaline Pectin Lyase from a Newly Isolated Bacillus clausii and Its Application in Elicitation of Plant Disease Resistance. APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY[J]. 2012, 167(8): 2241-2256, http://ir.rcees.ac.cn/handle/311016/7920.
[35] Hong, Y, Chen, Z, Zhang, B, Zhai, Q. Isolation of Photobacterium sp LuB-1 and its application in rapid assays for chemical toxicants in water. LETTERS IN APPLIED MICROBIOLOGY[J]. 2010, 51(3): 308-312, https://www.webofscience.com/wos/woscc/full-record/WOS:000280996100010.
[36] Zhang, Baoguo, Bai, Zhihui, Hoefel, Daniel, Tang, Ling, Wang, Xiaoyi, Li, Baoju, Li, Zuming, Zhuang, Guoqiang. The impacts of cypermethrin pesticide application on the non-target microbial community of the pepper plant phyllosphere. SCIENCEOFTHETOTALENVIRONMENT[J]. 2009, 407(6): 1915-1922, http://ir.rcees.ac.cn/handle/311016/21811.
[37] Zhang, Baoguo, Bai, Zhihui, Hoefel, Daniel, Tang, Ling, Yang, Zhiguang, Zhuang, Guoqiang, Yang, Jianzhou, Zhang, Hongxun. Assessing the impact of the biological control agent Bacillus thuringiensis on the indigenous microbial community within the pepper plant phyllosphere. FEMS MICROBIOLOGY LETTERS[J]. 2008, 284(1): 102-108, http://ir.rcees.ac.cn/handle/311016/22346.
[38] Effect of cypermethrin insecticide on the microbial community in cucumber phyllosphere. 环境科学学报英文版[J]. 2008, http://lib.cqvip.com/Qikan/Article/Detail?id=1000588745.
[39] Zhang Baoguo, Zhang Hongxun, Jin Bo, Tang Ling, Yang Jianzhou, Li Baoju, Zhuang Guoqiang, Bai Zhihui. Effect of cypermethrin insecticide on the microbial community in cucumber phyllosphere. JOURNAL OF ENVIRONMENTAL SCIENCES[J]. 2008, 20(11): 1356-1362, http://lib.cqvip.com/Qikan/Article/Detail?id=1000588745.
[40] 张保国. Assessing the impacts of the biological control agent Bacillus thuringiensis on the indigenous microbial community within the pepper plant phyllosphere. FEMS Microbiol Lett. 2008, 

（1） 应用微生物与微生物技术目前的研究、技术与教育, Current Research, Technology and Education Topics in Applied Microbiology and Microbial Biotechnology, Formatex Research Center, 2010-09, 第 5 作者

   

（ 1 ） 国家自然科学基金, 主持, 国家级, 2011-11--2014-12
（ 2 ） 光合细菌的规模化生产及在养殖上的推广应用, 主持, 省级, 2016-04--2018-03
（ 3 ） 微生物发酵法生产EPA菌种及工艺开发, 主持, 院级, 2013-10--2016-10
（ 4 ） 微生物转化植物甾醇制备雄烯二酮（AD）基因工程菌构建, 主持, 院级, 2014-06--2017-05
（ 5 ） 生物转化植物甾醇制备DHEA生产工艺的开发, 主持, 院级, 2014-06--2017-05
（ 6 ） 生物除臭菌剂开发, 主持, 院级, 2015-08--2018-07
（ 7 ） 禽及水产养殖专用光合菌剂开发, 主持, 院级, 2015-06--2018-05
（ 8 ） 农业废弃物资源化利用机制, 参与, 国家级, 2017-07--2020-12
（ 9 ） 农业生产副产物和废弃物高值化利用生物技术开发, 主持, 国家级, 2018-07--2021-06
（ 10 ） 微生物催化法制备α-熊果苷和红景天苷工艺开发, 主持, 院级, 2017-09--2020-09
（ 11 ） 生转催化法转化雄烯二酮（4-AD）制备雄二烯二酮（ADD）工艺开发, 主持, 院级, 2019-03--2024-03
（ 12 ） 农业有机废物资源化成套利用技术与模式示范, 参与, 部委级, 2020-01--2021-12

（1）微生物转化植物甾醇制备甾体药物关键中间体研究进展   2016甾体激素类原料及中间体产业与市场论坛   张保国   2016-04-16
（2）微藻脂代谢关键调控因子的发掘及其作用机制初步研究    微藻生物质能源技术创新与产业化推进研讨会   张保国   2012-03-17

已指导学生

杨成遇  硕士研究生  085238-生物工程  

现指导学生

刘相岑  博士研究生  081703-生物化工