General

Guanghui Liu, Ph.D, Prof.

  Principal Investigator

       State Key Laboratory of Membrane Biology, Institute of Zoology

  1) Using stem cell to study and treat human disease   

  2) Genetic and molecular bases of healthy aging

  3) Targeted genome editing  

  E-mail:ghliu@ioz.ac.cn, Tel:010-64889970

  Fax:010-64889970, Zip code:100101

Research Areas

1) Using stem cell to study and treat human disease

2) Genetic and molecular bases of healthy aging

3) Targeted genome editing

Education

1997-2002, B.S., Peking University Health Science Center

2002-2007, Ph.D., Institute of Biophysics, Chinese Academy of Sciences


  

Experience

2007-2009, Research associate, Dept. of Cell and Molecular Biology, The Scripps Research Institute

2009-2012, Research associate, Gene expression laboratory, The Salk Institute for Biological Studies

2012-, Professor, Institute of Biophysics, Chinese Academy of Sciences

2019-, Professor, Institute of Zoology, Chinese Academy of Sciences

Honors & Distinctions

2018, Tan Jiazhen prize in life sciences innovation
2018-, President, Chinese Society of Aging Cell Research (CSACR)
2018-, Associate Editor, Stem Cell Research & Therapy (Impact factor 4.9)
2017-, Vice president, Chinese Society of Aging Biology (CSAB)
2017, Second class Beijing Science and Technology Award (first place)
2017, Shulan Medical Prize
2017, Youth Scientist of Chinese Academy of Sciences
2017, CSCB Young Investigator
2017, Gerontology Outstanding Contribution Award
2017-, Editorial board, Cell Stress
2017-, Editorial board, Cell Proliferation
2017-, Editorial board, Life Science Alliance
2016-, Vice president, Chinese Society of Regenerative Cell Biology
2016, Innovative Award of “the capital of ten scientific and technological research and management plan”
2016, National Science Foundation for Distinguished Young Scholars
2015, 973 program chief scientist
2015, Bill and Melinda Gates Foundation Grand Challenges 2015-Young Scientist
2015, CSSCR Stem Cell Young Investigator
2015-, Deputy Editor-in-Chief, Protein & Cell (Impact factor 6.2)
2013, Bei Shi-Zhang Young Scientist Award in Biophysics
2012, Thousand Young Talents program of China
2012, National Science Foundation for Excellent Young Scholars
2011, Glenn Aging Foundation fellowship (in US)
2010, California Institute of Regenerative Medicine (CIRM) scholar training grant (in US)
2007, Award by the president of Chinese Academy of Sciences

Publications


1.    Wang S, Zheng Y, Li J, Yu Y, Zhang W, Song M, Liu Z, Min Z, Hu H, Jing Y, He X, Sun L, Esteban C, Chan P, Qiao J, Zhou Q, Belmonte JC*, Jing Qu*, Tang F*, Liu GH*. Single-Cell Transcriptomic Atlas of Primate Ovarian Aging. Cell. 2020. 180(3):585-600 (Cover story)

2.    Ma S, Sun S, Geng L, Song M, Wang W, Ye Y, Ji Q, Zou Z, Wang S, He X, Li W, Rodriguez Esteban C, Long X, Guo G, Chan P, Zhou Q, Belmonte JC*, Zhang W*, Qu J*, Liu GH*. Caloric Restriction Reprograms the Single-Cell Transcriptional Landscape of Rattus Norvegicus Aging. Cell.2020. 180(5):984-1001.

3.    Little M, Liu GH, Shenoy K, Vunjak-Novakovic G, Radisic M. Engineering Tissues and Organs: The Road to the Clinic. Cell. 2020. 181(1):22-23. DOI: 10.1016/j.cell.2020.03.026 (Voices)

4.    He X, Memczak S, Qu J*, Belmonte JC*, Liu GH*. Single-cell “OMICS” in aging: a young growing field. Nat Metab. 2020. 2: 293–302

5.    Liu X, Belmonte JCI, Zhang W*, Liu GH*. A betagalactosidase kiss of death for senescent cells. Cell Res. 2020. DOI:10.1038/s41422-020-0325-6

6.    Zhang W, Zhang S, Yan P, Ren J, Song M, Li J, Lei J, Pan H, Wang S, Ma X, Ma S, Li H,  Sun F, Wan H, Li W, Chan P, Zhou Q, Liu GH*, Tang F*, Qu J*. A Single-Cell Transcriptomic landscape of Primate Arterial Aging. Nat Commun. 2020. DOI: 10.1038/s41467-020-15997-0

7.    Li J, Zheng Y, Yan P, Song M, Wang S, Sun L, Liu Z, Ma S, Chan P, Zhou Q, Belmonte JC, Zhang W*, Liu GH*, Tang F*, Qu J,*. A Single-cell Transcriptomic Atlas of Primate Pancreatic Islet Aging. National Science Review. 2020. DOI: 10.1093/nsr/nwaa127

8.    Zhang W, Qu J, Liu GH*, Belmonte JC*. The Aging Epigenome and its Rejuvenation. Nat Rev Mol Cell Biol.2020. 21(3):137-150.

9.    Li H, Wu Z, Liu X, Zhang S, Ji Q, Jiang X, Liu Z, Wang S, Qu J, Zhang W, Song M*, Song E*, Liu GH*. ALKBH1 deficiency leads to loss of homeostasis in human diploid somatic cells. Protein Cell. 2020. DOI: 10.1007/s13238-020-00744-4

10.  Hu H, Ji Q, Song M, Ren J, Liu Z, Wang Z, Liu X, Yan K, Hu J, Wang S, Zhang W*, Liu GH*, Qu J*. ZKSCAN3 counteracts cellular senescence by stabilizing heterochromatin. Nucleic Acids Research. 2020. DOI: 10.1093/nar/gkaa425

11.  Bi S, Liu Z, Wu Z, Wang Z, Liu X, Wang S, Ren J, Zhang W*, Song M*, Liu GH*, Qu J*. SIRT7 antagonizes human stem cell aging as a heterochromatin stabilizer. Protein Cell. 2020. DOI: 10.1007/s13238-020-00728-4

12.  Wang F, Zhang W, Yang Q, Kang Y, Fan Y, Wei J, Liu Z, Dai S, Li H, Li Z, Xu L, Chu C, Qu J, Si C, Ji W*,Liu GH*, Long C*, Niu Y*. Generation of a Hutchinson-Gilford progeria syndrome monkey model by base editing. Protein Cell. 2020. In press

13.  Wang S, Min Z, Ji Q, Geng L, Su Y, Liu Z, Hu H, Wang L, Zhang W, Suzuiki K, Huang YZhang P, Tang T, Qu J*, Yu Y*, Liu GH*, Qiao J*. Rescue of Premature Aging Defects in Cockayne Syndrome Stem Cells by CRISPR/Cas9-mediated Gene Correction. Protein Cell. 2020. 11(1):1-22.

14.  Zhang J, Liu GH*, Qu J*, Song M*. Treating Osteoarthritis via Gene Therapy with Rejuvenation Factors. Gene Therapy. 2020. DOI: 10.1038/s41434-020-0149-5

15.  Martinez-Redondo P, Guillen-Guillen I, Davidsohn N, Wang C, Prieto J, Kurita M, Hatanaka F, Zhong C, Hernandez-Benitez R, Hishida T, Lezaki T, Sakamoto A, Nemeth AN, Hishida Y, Esteban CR, Shojima K, Huang L, Shokhirev M, Nuñez-Delicado E, Campistol JM, Guillen-Vicente I, Rodriguez-Iñigo E, Lopez-Alcorocho JM, Guillen-Vicente M, Church G, Reddy P, Guillen-Garcia P*, Liu GH*, Belmonte JCI*. αKLOTHO and sTGFβR2 treatment counteract the osteoarthritic phenotype developed in a rat model. Protein Cell. 2020. 11(3):219-226

16.  Yan P, Li Q, Wang L, Lu P, Suzuki K, Liu Z, Lei J, Li W, Ren R, He X, Wang S, Ding J, Chan P, Zhang W, Song M, Belmonte JC, Qu J#, Tang F#, Liu GH*. FOXO3-engineered human ESC-derived vascular cells promote vascular protection and regeneration. Cell Stem Cell. 2019. 24(3), 447-461

17.  Deng L, Ren R, Liu Z, Song M, Li J, Wu Z, Ren X, Fu L, Li W, Zhang W, Guillen P, Belmonte J, Chan P, Qu J#, Liu GH*. Stabilizing heterochromatin by DGCR8 alleviates senescence and osteoarthritis. Nat Commun. 2019. 10(1):3329.

18.  Fu L, Hu Y, Song M, Liu Z, Zhang W, Yu FX, Wu J, Wang S, Belmonte JC, Chan P, Qu J#, Tang F#,  Liu GH*. Upregulation of FOXD1 by YAP alleviates senescence and osteoarthritis. PLoS Biology. 2019. 17(4):e3000201

19.  Ren X, Hu B, Song M, Ding Z, Dang Y, Liu Z, Zhang W, Ji Q, Ren R, Ding J, Chan P, Jiang C, Ye K, Qu J#, Tang F#, Liu GH*. Maintenance of nucleolar homeostasis by CBX4 alleviates senescence and osteoarthritis. Cell Reports. 2019. 26(13):3643-3656.

20.  Ling C, Liu Z, Song M, Zhang W, Wang S, Liu X, Ma S, Sun S, Fu L, Chu Q, Belmonte JC, Wang Z, Qu J#, Yuan Y#, Liu GH*. Modeling CADASIL vascular pathologies with patient-derived induced pluripotent stem cells. Protein Cell. 2019. 10(4):249-271

21.  Cheng F, Wang S, Song M, Liu Z, Liu P, Chan P, Wang Y, Wang L, Zhang W*, Qu J*, Liu GH*. DJ-1 is dispensable for human stem cell homeostasis. Protein Cell. 2019. 10(11):846-853

22.  Geng L, Liu Z, Wang S, Sun S, Ma S, Liu X, Chan P, Sun L, Song M*, Zhang W*, Liu GH*, Qu J*. Low-dose quercetin positively regulates mouse healthspan. Protein Cell. 2019. 10(10):770-775.

23.  Song M, Belmonte JCI#, Liu GH*. Age-related cardiopathies gene editing. Aging (Albany NY). 2019. 11(5):1327-1328.

24.  Zhang X, Liu Z, Liu X, Wang S, Zhang Y, He X, Sun S, Ma S, Shyh-Chang N, Liu F, Wang Q, Wang X, Liu L, Zhang W*, Song M*, Liu GH*, Qu J*. Telomere-Dependent and Telomere-Independent Roles of RAP1 in Regulating Human Stem Cell Homeostasis. Protein Cell. 2019. 10(9):649-667.

25.  Geng L, Liu Z, Zhang W*, Li W, Wu Z, Wang W, Ren R, Su Y, Wang P, Ju Z, Chan P, Song M*, Qu J*, Liu GH*. Chemical Screen Identifies a Geroprotective Role of Quercetin in Premature Aging. Protein Cell. 2019. 10(6):417-435

26.  Hishida T, Vazquez-Ferrer E, Hishida-Nozaki Y, Sancho-Martinez I, Takahashi Y, Hatanaka F, Wu J, Ocampo A, Reddy P, Wu MZ, Gerken L, Shaw R, Esteban CR, Benner C, Nakagawa H, Garcia PG, Delicado EN, Castells A, Campistol JM, Liu GH*, Belmonte JC*. Mutations in foregut SOX2+ cells induce efficient proliferation via CXCR2 pathway. Protein Cell. 2019. 10(7):485-495.

27.  He X, Song M, Qu J, Guo Y, Cao H, Sun R*, Liu GH*, Shen Y*, Major Program Expert Group. Basic and Translational Aging Research in China: Present and Future. Protein Cell. 2019. 10(7):476-484.

28.  Zhang W, Wan H, Feng G, Qu J, Wang J, Jing Y, Ren R, Liu Z, Zhang L, Chen Z, Wang S, Zhao Y, Wang Z, Yuan Y, Zhou Q, Li W#, Liu GH*, Hu B#. SIRT6 deficiency results in developmental retardation in cynomolgus monkeys. Nature.2018. 560(7720):661-665.

29.  Zhang W, Song M, Qu J#,  Liu GH*. Epigenetic Modifications in Cardiovascular Aging and Diseases. Circ Res. 2018. 123(7):773-786.

30.  Geng L, Liu Z, Zhang W#, Li W, Wu Z, Wang W, Ren R, Su Y, Wang P, Ju Z, Chan P, Song M#, Qu J#, Liu GH*. Chemical Screen Identifies a Geroprotective Role of Quercetin in Premature Aging. Protein Cell. 2018. doi: 10.1007/s13238-018-0567-y.

31.  Wang S, Hu B, Ding Z, Dang Y, Wu J, Li D, Liu X, Xiao B, Zhang W, Ren R, Lei J, Hu H, Chen C, Chan P, Li D, Qu J#, Tang F#, Liu GH*. ATF6 safeguards organelle homeostasis and cellular aging in human mesenchymal stem cells. Cell Discovery. 2018. Jan 5;4:2.

32.  Wu Z, Zhang W, Song M, Wang W, Wei G, Li W, Lei J, Huang Y, Sang Y, Chan P, Chen C, Qu J#, Suzuki K#, Belmonte JC#,  Liu GH*. Differential stem cell aging kinetics in Hutchinson–Gilford progeria syndrome and Werner syndrome. Protein Cell. 2018. 9(4):333-350.

33.  Fang J, Yang J, Wu X, Zhang G, Li T, Zhang H, Wang CC,  Liu GH*, Wang L#. Metformin alleviates human cellular aging by upregulating the ER peroxidase GPx7. Aging Cell.2018.17(4):e12765.

34.  Ren R, Deng L, Xue Y, Suzuki K, Zhang W, Yu Y, Wu J, Sun L, Gong X, Luan H, Yang F, Ju Z, Ren X, Wang S, Tang H, Geng L, Zhang W, Li J, Qiao J, Xu T#, Qu J#, Liu GH*. Visualization of aging-associated chromatin alterations with an engineered TALE system. Cell Res. 2017. 27(4):483-504

35.  Yang J, Li J, Suzuki K, Liu X, Wu J, Zhang W, Ren R, Zhang W, Chan P, Belmonte JC, Qu J#, Tang F#,  Liu GH*. Genetic enhancement in cultured human adult stem cells conferred by a single nucleotide recoding. Cell Res. 2017. 27(9):1178-1181

36.  Ren R, Ocampo A,  Liu GH*, Belmonte JC#. Regulation of Stem Cell Aging by Metabolism and Epigenetics. Cell Metab. 2017. 26(3):460-474.

37.  Wang L, Yi F, Fu L, Yang J, Wang S, Wang Z, Suzuki K, Sun L, Xu X, Yu Y, Qiao J, Belmonte JC, Yang Z, Yuan Y#, Qu J#,  Liu GH*. CRISPR/Cas9-mediated targeted gene correction in amyotrophic lateral sclerosis patient iPSCs. Protein Cell. 2017. 8(5):365-378

38.  Kubben N, Zhang W, Wang L, Voss T, Yang J, Qu J,  Liu GH*, Misteli T#, Repression of the antioxidant NRF2 pathway in premature aging. Cell. 2016. 165(6):1361-74

39.  Pan H, Guan D, Liu X, Li J, Wang L, Wu J, Zhou J, Zhang W, Ren R, Zhang W, Li Y, Yang J, Hao Y, Yuan T, Yuan G, Wang H, Ju Z, Mao Z, Li J, Qu J#, Tang F#,  Liu GH*. SIRT6 safeguards human mesenchymal stem cells from oxidative stress by coactivating NRF2. Cell Res. 2016. 26(2):190-205.

40.  Fu L, XX, Ren R, Wu J, Zhang W, Yang J, Ren X, Wang S, Zhao Y, Sun L, Yu Y, Wang Z, Yang Z, Yuan Y, Qiao J, Belmonte JC#, Qu J#,  Liu GH*. Modeling Xeroderma Pigmentosum associated neurological pathologies with patients-derived iPSCs. Protein Cell. 2016.7(3):210-21.

41.  Li Y, Zhang W, Chang L, Han Y, Sun L, Guo X, Tang H, Liu Z, Deng H, Ye Y, Ren R, Wang Y, Li J, Qiao J, Qu J#, Zhang W#,  Liu GH*. Vitamin C alleviates aging defects in a stem cell model for Werner syndrome. Protein Cell.2016. 7(7):478-88.

42.  Zhang W, Li J, Suzuki K, Qu J, Wang P, Zhou J, Liu X, Ren R, Xu X, Ocampo A, Yuan T, Yang J, Li Y, Shi L, Guan D, Pan H, Duan S, Ding Z, Li M, Yi F, Bai R, Wang Y, Chen C, Yang F, Li X, Wang Z, Aizawa E, Goebl A, Soligalla RD, Reddy P, Esteban CR, Tang F#,  Liu GH*, Belmonte JC#. A Werner syndrome stem cell model unveils heterochromatin alterations as a driver of human aging. Science. 2015; 348(6239):1160-3.

43.  Duan S, Yuan G, Liu X, Ren R, Li J, Zhang W, Wu J, Xu X, Fu L, Li Y, Yang J, Zhang W, Bai R, Yi F, Suzuki K, Gao H, Esteban CR, Zhang C, Belmonte JC, Chen Z, Wang X, Jiang T, Qu J#, Tang F#,  Liu GH*. PTEN deficiency reprograms human neural stem cells towards a glioblastoma stem cell-like phenotype. Nat Commun.2015. 6:10068.

44.  Suzuki K, Yu C, Qu J, Li M, Yao X, Yuan T, Goebl A, Tang S, Ren R, Aizawa E, Zhang F, Xu X, Soligalla R, Chen F, Kim J, Kim NY, Liao HK, Benner C, Esteban CR, Jin Y, Liu GH*, Li Y#, Belmonte JC#. Targeted gene correction in human disease-specific induced pluripotent stem cells minimally impacts whole-genome mutational load. Cell Stem Cell. 2014; 15, 31–36. 

45.   Liu GH*, Suzuki K, Li M, Qu J, Montserrat N, Tarantino C, Gu Y, Yi F, Xu X, Zhang W, Ruiz S, Plongthongkum N, Zhang K, Masuda S, Nivet E, Tsunekawa Y, Soligalla R, Goebl A, Aizawa E, Kim N, Kim J, Dubova I, Li Y, Ren R, Benner C, del Sol A, Bueren J, Trujillo J, Surralles J, Esteban C, Izpisua Belmonte JC#. Modeling Fanconi Anemia pathogenesis and therapeutics using integration-free patient iPSCs. Nat Commun. 2014. 7;5:4330. 

46.  Yang J, Cai N, Yi F,  Liu GH*, Qu J#, Belmonte JC#. Gating pluripotency via nuclear pores. Trends Mol Med. 2014. 20(1):1-7.

47.  Wu K, Ren R, Su W, Wen Bo, Zhang Yuying, Yi Fei, Qiao X, Yuan T, Wang J, Liu L, Belmonte JC#,  Liu GH*, Chen C#. A novel suppressive effect of alcohol dehydrogenase 5 in neuronal differentiation. J Biol Chem. 2014. 289(29):20193-9.

48.  Xu XL#, Yang JP, Fu LN, Ren RT, Yi F, Suzuki K, Liu K, Ding ZC, Qu J, Zhang WQ, Li Y, Yuan TT, Yuan GH, Sui LN, Guan D, Duan SL, Pan HZ, Wang P, Zhu XP, Montserrat N, Li M, Bai RJ, Liu L, Izpisua Belmonte JC,  Liu GH*. Direct reprogramming of porcine fibroblasts to neural progenitor cells. Protein Cell. 2014. 5(1):4-7.

49.  Xu X, Duan S, Yi F, Ocampo A,  Liu GH*, Belmonte JC#. Mitochondrial regulation in pluripotent stemcells. Cell Metab. 2013. 18:325-32.

50.  Pan H, Cai N, Li M,  Liu GH*, Belmonte JC#. Autophagic control of cell stemness. EMBO Mol. Med.2013 5(3):327-31.

51.  Zhang WQ, Qu J, Suzuki K,  Liu GH*, Belmonte JC#. Concealing cellular defects in pluripotent stem cells. Trends Cell Biol. 2013. 23(12):587-92.

52.  Guan D, Zhang W, Zhang W#,  Liu GH*, Belmonte JC#. Switching cell fate, ncRNAs coming to play. Cell Death Dis. 2013.17;4:e464.

53.  Sui L,  Liu GH*, Belmonte JC#. hESC-derived pancreatic progenitors. Cell Res. 2013. 23(5):592-4.

54.  Zhang K, Yi F,  Liu GH*, Belmonte JC#. New march towards the regeneration of sensation and cognition: hear more, see more and learn more. J Mol Cell Biol. 2013; 5(2):151-3.

55.  Liu W, Li M, Qu J, Yi F#,  Liu GH*.Reevaluation of the safety of induced pluripotent stem cells: a call from somatic mosaicism. Protein Cell. 2013; 4(2):83-5.

56.  Liu GH*, Qu J, Suzuki K, Nivet E, Li M, Montserrat N, Yi F, Xu X, Ruiz S, Zhang W, Ren B, Wagner U, Kim A, Li Y, Goebl A, Kim J, Soligalla R, Dubova I, Thompson J, Yates J III, Esteban C, Sancho-Martinez I, Belmonte JC#. Progressive degeneration of human neural stem cells caused by pathogenic LRRK2. Nature. 2012; 491(7425):603-7. 

57.  Li M*, Liu GH*, Belmonte JC. Navigating the epigenetic landscape of pluripotent stem cells. Nat Rev Mol Cell Biol. 2012; 13(8):524-35. (*equal contribution)

58.  Liu GH*, Ding Z, Izpisua Belmonte JC#. iPSC technology to study human aging and aging-related disorders. Curr Opin Cell Biol. 2012; 24(6):765-74.

59.  Liu GH*, Li M, Qu J, Belmonte JC#. Gating neural development and aging via nuclear pores. Cell Res. 2012; 22(8):1212-4.

60.  Wang P, Zhang W, Yang J, Qu J#,  Liu GH*. Higher-order genomic organization in pluripotent stem cells. Protein Cell. 2012; 3(7):483-6.

61.  Liu GH*, Suzuki K*, Qu J*, Sancho-Martinez I, Yi F, Li M, Kumar S, Nivet E, Kim J, Soligalla RD, Dubova I, Goebl A, Plongthongkum N, Fung HL, Zhang K, LoringJ,Laurent L, and Belmonte JC. Targeted gene correction of laminopathy-associated LMNA mutations in patient-specific iPSCs. Cell Stem Cell. 2011; 8(6):688-94. (*equal contribution)

62.  Pan H, Zhang W, Zhang W#,  Liu GH*.Find and Replace: editing human genome in pluripotent stem cells. Protein Cell. 2011; 2(12):950-6. 

63.  Liu GH, Barkho BZ, Ruiz S, Diep D, Qu J, Yang SL, Panopoulos AD, Suzuki K, Kurian L, Walsh C, Thompson J, Boue S, Fung HL, Sancho-Martinez I, Zhang K, III JY, Belmonte JC#. Recapitulation of premature ageing with iPSCs from Hutchinson-Gilford progeria syndrome. Nature. 2011; 14;472: 221-5


 

Book chapter

 Liu GH*, Yang J, Ding Z, Ocampo A, Qu J, Suzuki K, Izpisua Belmonte JC#. Chapter: iPSC disease modeling of laminopathies. Human iPS Cells in Disease Modeling (Springer). 2016. pp 53-67    

 

Patents


Research Interests

Dr. Guang-Hui Liu’s work focuseson using pluripotent stem cell to study and treat aging-associated disorders including neurodegenerative diseases.