基本信息
顾阳  男  硕导  中国科学院分子植物科学卓越创新中心
电子邮件: ygu@cemps.ac.cn
通信地址: 上海市枫林路300号2号楼1111
邮政编码: 200032

招生信息

   
招生专业
071005-微生物学
086000-生物与医药
招生方向
微生物代谢调控和代谢工程
微生物代谢调控和代谢工程

教育背景

2000-09--2006-06   南京林业大学   博士

工作经历

   
工作简历
2020-05~现在, 中国科学院分子植物科学卓越创新中心, 研究员
2014-12~2020-04,中国科学院上海生命科学研究院, 研究员
2011-03~2011-06,英国诺丁汉大学, 访问学者
2009-08~2014-11,中科院上海生命科学研究院植生所, 副研究员(2009年11月至今);硕导(2010年11月至今)
2006-07~2009-07,中科院上海生命科学研究院, 博士后
2000-09~2006-06,南京林业大学, 博士
社会兼职
2022-01-01-今,中国生物工程学会一碳生物技术专委会副主任委员,
2020-03-01-今,上海市生物工程学会青年工作委员会副主任委员,
2018-11-01-2022-11-30,上海市生物工程学会合成生物学委员会委员,
2016-12-31-2022-01-31,中国化工学会青年学者工作委员会常务委员,

教授课程

微生物基因组编辑技术进展
微生物系统与合成生物学

专利与奖励

   
专利成果
( 1 ) 一种提高合成气发酵产乙醇含量的突变株和利用突变株的应用, 2021, 第 2 作者, 专利号: CN113234653A

( 2 ) 丙酮丁醇梭菌中的一个非编码RNA及其应用, 2021, 第 1 作者, 专利号: CN110734909B

出版信息

   
发表论文
(1) Protein acetylation-mediated cross regulation of acetic acid and ethanol synthesis in the gas-fermenting Clostridium ljungdahlii, JOURNAL OF BIOLOGICAL CHEMISTRY, 2022, 通讯作者
(2) Functional dissection and modulation of the BirA protein for improved autotrophic growth of gas-fermenting Clostridium ljungdahlii, MICROBIAL BIOTECHNOLOGY, 2021, 通讯作者
(3) Metabolic Engineering of Gas-Fermenting Clostridium ljungdahlii for Efficient Co-production of Isopropanol, 3-Hydroxybutyrate, and Ethanol, ACS SYNTHETIC BIOLOGY, 2021, 通讯作者
(4) Discovery of an ene-reductase for initiating flavone and flavonol catabolism in gut bacteria, NATURE COMMUNICATIONS, 2021, 通讯作者
(5) Ethanol Metabolism Dynamics in Clostridium ljungdahlii Grown on Carbon Monoxide, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2020, 通讯作者
(6) Effect of temperature and surfactant on biomass growth and higher-alcohol production during syngas fermentation by Clostridium carboxidivorans P7, BIORESOURCES AND BIOPROCESSING, 2020, 第 5 作者
(7) The Small RNA sr8384 Is a Crucial Regulator of Cell Growth in Solventogenic Clostridia, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2020, 通讯作者
(8) A novel regulatory pathway consisting of a two-component system and an ABC-type transporter contributes to butanol tolerance in Clostridium acetobutylicum, APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2020, 通讯作者
(9) Developing an endogenous quorum-sensing based CRISPRi circuit for autonomous and tunable dynamic regulation of multiple targets in Streptomyces, NUCLEIC ACIDS RESEARCH, 2020, 第 3 作者
(10) Interactive Regulation of Formate Dehydrogenase during CO 2 Fixation in Gas-Fermenting Bacteria, mBio, 2020, 通讯作者
(11) Ethanol Metabolism Dynamics in Clostridium ljungdahlii Grown on Carbon Monoxide (vol 14, e00730, 2020), APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2020, 通讯作者
(12) Engineering Clostridium ljungdahlii as the gas-fermenting cell factory for the production of biofuels and biochemicals, CURRENT OPINION IN CHEMICAL BIOLOGY, 2020, 通讯作者
(13) The Metabolism of Clostridium ljungdahlii in Phosphotransacetylase Negative Strains and Development of an Ethanologenic Strain, FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY, 2020, 第 7 作者
(14) Control of solvent production by sigma‐54 factor and the transcriptional activator AdhR in Clostridium beijerinckii, Microbial Biotechnology, 2020, 第 3 作者
(15) CRISPR-Cas12a-Mediated Gene Deletion and Regulation in Clostridium ljungdahlii and Its Application in Carbon Flux Redirection in Synthesis Gas Fermentation, ACS SYNTHETIC BIOLOGY, 2019, 通讯作者
(16) Metabolic engineering of Escherichia coli carrying the hybrid acetone-biosynthesis pathway for efficient acetone biosynthesis from acetate, MICROBIAL CELL FACTORIES, 2019, 第 4 作者
(17) Phage serine integrase-mediated genome engineering for efficient expression of chemical biosynthetic pathway in gas-fermenting Clostridium ljungdahlii, METABOLIC ENGINEERING, 2019, 通讯作者
(18) Generation of a fully erythromycin-sensitive strain of Clostridioides difficile using a novel CRISPR-Cas9 genome editing system, SCIENTIFIC REPORTS, 2019, 第 8 作者
(19) A Novel Dual- cre Motif Enables Two-Way Autoregulation of CcpA in Clostridium acetobutylicum, APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2018, 通讯作者
(20) Metabolic regulation in solventogenic clostridia: regulators, mechanisms and engineering, BIOTECHNOLOGY ADVANCES, 2018, 通讯作者
(21) A Flexible Binding Site Architecture Provides New Insights into CcpA Global Regulation in Gram-Positive Bacteria, mBio, 2017, 通讯作者
(22) Rapid Generation of Universal Synthetic Promoters for Controlled Gene Expression in Both Gas-Fermenting and Saccharolytic Clostridium Species, ACS Synthetic Biology, 2017, 第 6 作者
(23) CRISPR/Cas9-Based Efficient Genome Editing in Clostridium ljungdahlii , an Autotrophic Gas-Fermenting Bacterium, ACS Synthetic Biology, 2016, 
(24) Improving the performance of solventogenic clostridia by reinforcing the biotin synthetic pathway, METABOLIC ENGINEERING, 2016, 通讯作者
(25) Comparative genomic and transcriptomic analysis revealed genetic characteristics related to solvent formation and xylose utilization in Clostridium acetobutylicum EA 2018, BMC Genomics, 2011, 第 4 作者
(26) Identification and inactivation of pleiotropic regulator CcpA to eliminate glucose repression of xylose utilization in Clostridium acetobutylicum, METABOLIC ENGINEERING, 2010, 第 2 作者
(27) Reconstruction of xylose utilization pathway and regulons in Firmicutes, BMC GENOMICS, 2010, 第 1 作者
(28) Ammonium acetate enhances solvent production by Clostridium acetobutylicum EA 2018 using cassava as a fermentation medium, JOURNAL OF INDUSTRIAL MICROBIOLOGY & BIOTECHNOLOGY, 2009, 第 1 作者
(29) Improvement of xylose utilization in Clostridium acetobutylicum via expression of the talA gene encoding transaldolase from Escherichia coli, JOURNAL OF BIOTECHNOLOGY, 2009, 

科研活动

   
科研项目
( 1 ) 丁醇工业生产菌株的基因工程改良和产业化示范, 负责人, 地方任务, 2011-01--2012-12
( 2 ) 产溶剂梭菌木糖代谢途径解析及分子改造, 负责人, 中国科学院计划, 2010-08--2012-07
( 3 ) 人工合成细胞工厂, 参与, 国家任务, 2011-01--2015-12
( 4 ) 小蛋白介导的梭菌溶剂合成调控机制研究, 负责人, 国家任务, 2017-01--2020-12
( 5 ) 中国科学院青年创新促进会优秀会员人才专项基金, 负责人, 中国科学院计划, 2017-01--2019-12
( 6 ) 双功能蛋白BirA在食气永达尔梭菌化能固碳中的作用机制研究, 负责人, 国家任务, 2020-01--2023-12
( 7 ) 有机碳一原料高效生物利用机制研究, 负责人, 国家任务, 2019-07--2024-06
( 8 ) 工业含碳尾气生物转化制长碳链化合物的关键技术研发、集成 与示范, 负责人, 地方任务, 2021-11--2024-10
参与会议
(1)肠道菌新型黄酮还原酶的发现及其生理功能解析   2021中国肠道大会   2021-05-28
(2)生物转化一碳气体合成长碳链化合物的分子操作技术体系创新与应用   2018年中国微生物学会学术年会   2018-10-11
(3)Design and Application of Clostridial Cell Factories: from Carbohydrates to C1 Gases   2017-07-01
(4)Engineering Gas-Fermenting Clostridia for Production of Chemicals and Biofuels   2016-08-28