1. 纤毛形成与解聚的分子机制；2.雪藻驱动极地碳循环及适应低温和高辐射的机制

1. 药用单细胞真核微藻的设计构建；2. 微藻硒蛋白的生物合成及有机硒产品的开发

   

071007-遗传学
071009-细胞生物学
071004-水生生物学

纤毛的形成与解聚
合成生物学
微藻固碳及在环境修复中的应用

2020年本实验室计划在藻类合成生物学方向招收博士生1名、硕士生1名；在藻类细胞生物学方向招收硕士生1名。欢迎优秀本科毕业生报考。

1998-10--2004-04   德国Freibueg 大学   博士

   

2011-01~现在, 中科院水生生物研究所, 研究员
2009-09~2010-12,耶鲁大学, 合作研究科学家
2004-05~2009-08,耶鲁大学, 博士后

分子细胞生物学
细胞生物学

   

[1] Huan Long, Jinhua Fang, Lian Ye, Zhang, Baolong, Colleen Hui, Xuan Deng, Sabeeha S. Merchant, Kaiyao Huang. Structural and functional regulation of Chlamydomonas lysosome-related organelles during environmental changes. Plant Physiology[J]. 2023, [2] Zhang, Baolong, Tang, Yuxin, Yu, Fei, Peng, Zhao, Yao, Sheng, Deng, Xuan, Huan Long, Wang, Xun, Kaiyao Huang. Translatomics and physiological analyses of the detoxification mechanism of green alga Chlamydomonas reinhardtii to cadmium toxicity. JOURNAL OF HAZARDOUS MATERIALS[J]. 2023, 448: http://dx.doi.org/10.1016/j.jhazmat.2023.130990.
[3] Yuxing Tang, Zhang, Baolong, Huan Long, Kaiyao Huang. Overexpression of the sulfate transporter-encoding SULTR2 increases chromium accumulation in Chlamydomonas reinhardtii. Biotechnology and Bioengineering.. Biotechnology and Bioengineering[J]. 2023, [4] 周洪, 魏凤, Kaiyao Huang, 石德太. 基于专利分析的硒医药技术发展研究. 中国现代应用药学[J]. 2021, 38(03期): 377-384, VIP_JournalArticle.
[5] Peng, Zhao, Liu, Gai, Kaiyao Huang. Cold Adaptation Mechanisms of a Snow Alga Chlamydomonas nivalis During Temperature Fluctuations. FRONTIERS IN MICROBIOLOGY[J]. 2021, 11: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7874021/.
[6] Zhang, Baolong, Duan, Guangqian, Fang, Yingying, Deng, Xuan, Yin, Yongguang, Kaiyao Huang. Selenium(IV) alleviates chromium(VI)-induced toxicity in the green alga Chlamydomonas reinhardtii. ENVIRONMENTAL POLLUTION[J]. 2021, 272: https://www.webofscience.com/wos/woscc/full-record/WOS:000615555000106.
[7] Kaiyao Huang. Heme-binding protein CYB5D1 is a novel radial spoke component required for coordinated ciliary beating. Proc. Natl. Acad. Sci. 2021, [8] Yang, Huihui, Zhang, Fan, 龙欢, Lin, Yiwen, Liao, Jiahui, Xia, Haibin, Kaiyao Huang. IFT20 Mediates the Transport of Cell Migration Regulators From the Trans-Golgi Network to the Plasma Membrane in Breast Cancer Cells. FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY[J]. 2021, 9: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7968458/.
[9] Miao Rongli, Huang Kaiyao. Cyclic adenosine monophosphate signal pathway is involved in regulation of triacylglycerol biosynthesis following nitrogen deprivation in Chlamydomonas reinhardtii. JOURNAL OF OCEANOLOGY AND LIMNOLOGY[J]. 2020, 38(2): 517-528, http://lib.cqvip.com/Qikan/Article/Detail?id=7101034204.
[10] Luo, Wei, Ding, Haitao, Li, Huirong, Ji, Zhongqiang, Huang, Kaiyao, Zhao, Wenyu, Yu, Yong, Zeng, Yinxin. Molecular diversity of the microbial community in coloured snow from the Fildes Peninsula (King George Island, Maritime Antarctica). POLAR BIOLOGY[J]. 2020, 43(9): 1391-1405, https://www.webofscience.com/wos/woscc/full-record/WOS:000546876100001.
[11] Ma, Xiaocui, Zhang, Baolong, Miao, Rongli, Deng, Xuan, Duan, You, Cheng, Yingyin, Zhang, Wanting, Shi, Mijuan, Huang, Kaiyao, Xia, XiaoQin. Transcriptomic and Physiological Responses to Oxidative Stress in a Chlamydomonas reinhardtii Glutathione Peroxidase Mutant. GENES[J]. 2020, 11(4): http://apps.webofknowledge.com/CitedFullRecord.do?product=UA&colName=WOS&SID=5CCFccWmJJRAuMzNPjj&search_mode=CitedFullRecord&isickref=WOS:000537224600073.
[12] Yang, Huihui, Huang, Kaiyao. Dissecting the Vesicular Trafficking Function of IFT Subunits. FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY[J]. 2020, 7: https://doaj.org/article/b22fa23517b747309f9f118878783a30.
[13] Xu, Nannan, Oltmanns, Anne, Zhao, Longsheng, Girot, Antoine, Karimi, Marzieh, Hoepfner, Lara, Kelterborn, Simon, Scholz, Martin, Beissel, Julia, Hegemann, Peter, Baeumchen, Oliver, Liu, LuNing, Huang, Kaiyao, Hippler, Michael. Altered N-glycan composition impacts flagella-mediated adhesion in Chlamydomonas reinhardtii. ELIFE[J]. 2020, 9: https://doaj.org/article/7a39d2e759cc4004bde99b9fb5b319ff.
[14] 龙欢, Huang, Kaiyao. Transport of Ciliary Membrane Proteins. FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY[J]. 2020, 7: https://doaj.org/article/b7e915467b1a45c59108851cf30d90e3.
[15] Kaiyao Huang. Cold adaptation mechanisms of Chlamydomonas nivalis during temperature fluctuation.. Frontiers in Microbiology. 2020, [16] Jia, Ru, Chai, Yongping, Xie, Chao, Liu, Gai, Zhu, Zhiwen, Huang, Kaiyao, Li, Wei, Ou, Guangshuo. The spectrin-based membrane skeleton is asymmetric and remodels during neural development in C. elegans. JOURNAL OF CELL SCIENCE[J]. 2020, 133(15): http://dx.doi.org/10.1242/jcs.248583.
[17] Miao, Rongli, Ma, Xiaocui, Deng, Xuan, Huang, Kaiyao. High level of reactive oxygen species inhibits triacylglycerols accumulation in Chlamydomonas reinhardtii. ALGAL RESEARCH-BIOMASS BIOFUELS AND BIOPRODUCTS[J]. 2019, 38: http://dx.doi.org/10.1016/j.algal.2018.101400.
[18] 段光前, 李硕硕, 李鑫, 黄开耀. 裂殖壶菌高产油突变体的高通量筛选. 生物工程学报[J]. 2019, 35(7): 1335-1347, http://lib.cqvip.com/Qikan/Article/Detail?id=7002704199.
[19] Kaiyao Huang. Polyubiquitylation of a-tubulin at K304 is required for flagellar disassembly in Chlamydomonas. J Cell Sci. 2019, [20] Kaiyao Huang. A vitamin-C-derived DNA modification catalyzed by an algal TET homologue. Nature. 2019, [21] Xue, JianHuang, Chen, GuoDong, Hao, Fuhua, Chen, Hui, Fang, Zhaoyuan, Chen, FangFang, Pang, Bo, Yang, QingLin, Wei, Xinben, Fan, QiangQiang, Xin, Changpeng, Zhao, Jiaohong, Deng, Xuan, Wang, BangAn, Zhang, XiaoJie, Chu, Yueying, Tang, Hui, Yin, Huiyong, Ma, Weimin, Chen, Luonan, Ding, Jianping, Weinhold, Elmar, Kohli, Rahul M, Liu, Wen, Zhu, ZhengJiang, Huang, Kaiyao, Tang, Huiru, Xu, GuoLiang. A vitamin-C-derived DNA modification catalysed by an algal TET homologue. NATURE[J]. 2019, 569(7757): 581-+, [22] Wang, Qiyu, Peng, Zhao, Long, Huan, Deng, Xuan, Huang, Kaiyao. Polyubiquitylation of alpha-tubulin at K304 is required for flagella disassembly in Chlamydomonas. JOURNAL OF CELL SCIENCE[J]. 2019, 132(6): https://www.webofscience.com/wos/woscc/full-record/WOS:000462864300020.
[23] Zhang, Weiqing, Zhong, Huan, Lu, Hui, Zhang, Yuxiang, Deng, Xuan, Huang, Kaiyao, Duanmu, Deqiang. Characterization of Ferredoxin-Dependent Biliverdin Reductase PCYA1 Reveals the Dual Function in Retrograde Bilin Biosynthesis and Interaction With Light-Dependent Protochlorophyllide Oxidoreductase LPOR in Chlamydomonas reinhardtii. FRONTIERS IN PLANT SCIENCE[J]. 2018, 9(1): https://doaj.org/article/1e63eeaaf3164f3d9128cf14f23971ec.
[24] Schulze, Stefan, Oltmanns, Anne, Machnik, Nick, Liu, Gai, Xu, Nannan, Jarmatz, Niklas, Scholz, Martin, Sugimoto, Kazuhiko, Fufezan, Christian, Huang, Kaiyao, Hippler, Michael. N-Glycoproteomic Characterization of Mannosidase and Xylosyltransferase Mutant Strains of Chlamydomonas reinhardtii. PLANT PHYSIOLOGY[J]. 2018, 176(3): 1952-1964, http://www.irgrid.ac.cn/handle/1471x/1760377.
[25] Chen, Tianbing, Song, Guili, Yang, Huihui, Mao, Lin, Cui, Zongbin, Huang, Kaiyao. Development of the Swimbladder Surfactant System and Biogenesis of Lysosome-Related Organelles Is Regulated by BLOS1 in Zebrafish. GENETICS[J]. 2018, 208(3): 1131-1146, http://www.irgrid.ac.cn/handle/1471x/1760351.
[26] Dong, Bin, Wu, Song, Wang, Jing, Liu, YanXia, Peng, Zhao, Meng, DeMei, Huang, Kaiyao, Wu, Mingfu, Fan, ZhenChuan. Chlamydomonas IFT25 is dispensable for flagellar assembly but required to export the BBSome from flagella. BIOLOGY OPEN[J]. 2017, 6(11): 1680-1691, http://www.irgrid.ac.cn/handle/1471x/1760308.
[27] Lv, Bo, Wan, Lei, Taschner, Michael, Cheng, Xi, Lorentzen, Esben, Huang, Kaiyao. Intraflagellar transport protein IFT52 recruits IFT46 to the basal body and flagella. JOURNAL OF CELL SCIENCE[J]. 2017, 130(9): 1662-1674, http://dx.doi.org/10.1242/jcs.200758.
[28] Huan Long, 黄开耀, Joel LRosenbaum, Dennis RDiener, Gai Liu, Nannan Xu, Fan Zhang. 纤毛膜与纤毛囊泡的比较分析. 科学新闻[J]. 2017, 128-128, http://lib.cqvip.com/Qikan/Article/Detail?id=672078113.
[29] 徐泽东, 高其双, 蒋思文, 王利燕, 黄开耀, 彭霞, 桑奥闻. 3株真核微藻在不同浓度猪粪水沼液中生长差异及氮磷去除率研究. 中国畜牧杂志[J]. 2017, 53(7): 98-102, http://lib.cqvip.com/Qikan/Article/Detail?id=672666458.
[30] Cheng, Xi, Liu, Gai, Ke, Wenting, Zhao, Lijuan, Lv, Bo, Ma, Xiaocui, Xu, Nannan, Xia, Xiaoling, Deng, Xuan, Zheng, Chunlei, Huang, Kaiyao. Building a multipurpose insertional mutant library for forward and reverse genetics in Chlamydomonas. PLANT METHODS[J]. 2017, 13(1): http://ir.ihb.ac.cn/handle/342005/29186.
[31] Hochmal, Ana Karina, Zinzius, Karen, Charoenwattanasatien, Ratana, Gaebelein, Philipp, Mutoh, Risa, Tanaka, Hideaki, Schulze, Stefan, Liu, Gai, Scholz, Martin, Nordhues, Andre, Offenborn, Jan Niklas, Petroutsos, Dimitris, Finazzi, Giovanni, Fufezan, Christian, Huang, Kaiyao, Kurisu, Genji, Hippler, Michael. Calredoxin represents a novel type of calcium-dependent sensor-responder connected to redox regulation in the chloroplast. NATURE COMMUNICATIONS[J]. 2016, 7(1): https://doaj.org/article/b02d59660bf848dea61046fdbac4f8df.
[32] Chen, Ji, Cui, Xiaojuan, Jia, Shaoting, Luo, Daji, Cao, Mengxi, Zhang, Yunsheng, Hu, Hongling, Huang, Kaiyao, Zhu, Zuoyan, Hu, Wei. Disruption of dmc1 Produces Abnormal Sperm in Medaka (Oryzias latipes). SCIENTIFIC REPORTS[J]. 2016, 6: https://www.webofscience.com/wos/woscc/full-record/WOS:000380639200002.
[33] 黄开耀, 王亚丽, 徐泽东, 蒋思婧. 小球藻细胞壁缺陷型突变体的筛选及转化系统的建立. 水生生物学报[J]. 2016, 40(2): 370-377, http://lib.cqvip.com/Qikan/Article/Detail?id=668616677.
[34] Long, Huan, Zhang, Fan, Xu, Nannan, Liu, Gai, Diener, Dennis R, Rosenbaum, Joel L, Huang, Kaiyao. Comparative Analysis of Ciliary Membranes and Ectosomes. CURRENT BIOLOGY[J]. 2016, 26(24): 3327-3335, http://dx.doi.org/10.1016/j.cub.2016.09.055.
[35] Gao, Xiang, Zhang, Fengge, Hu, Jinlu, Cai, Wenkai, Shan, Ge, Dai, Dongsheng, Huang, Kaiyao, Wang, Gaohong. MicroRNAs modulate adaption to multiple abiotic stresses in Chlamydomonas reinhardtii. SCIENTIFIC REPORTS[J]. 2016, 6: https://www.webofscience.com/wos/woscc/full-record/WOS:000389122400001.
[36] Wang, Runfa, Peng, Bo, Huang, Kaiyao. The research progress of CO2 sequestration by algal bio-fertilizer in China. JOURNAL OF CO2 UTILIZATION[J]. 2015, 11: 67-70, http://dx.doi.org/10.1016/j.jcou.2015.01.007.
[37] 江娴, 王润发, 黄开耀, 魏凤. 基于文献计量学的中国生物固碳领域研发态势分析. 中国科学基金[J]. 2015, 29(6): 449-456, http://ir.ihb.ac.cn/handle/342005/28276.
[38] 刘盖, 黄开耀. 磷酸化调控纤毛的解聚. 中国科学. 生命科学[J]. 2015, 45(7): 706-708, https://www.sciengine.com/doi/10.1007/s11427-015-4874-8-94.
[39] Liu Gai, Huang KaiYao. Phosphorylation regulates the disassembly of cilia. SCIENCE CHINA-LIFE SCIENCES[J]. 2015, 58(6): 621-623, http://www.irgrid.ac.cn/handle/1471x/979242.
[40] Long, Huan, Wang, Qiyu, Huang, Kaiyao. Ciliary/Flagellar Protein Ubiquitination. CELLS[J]. 2015, 4(3): 474-482, https://doaj.org/article/af34223ddb3e484aa27a7a041605d6cb.
[41] Hu, Jinlu, Deng, Xuan, Shao, Ning, Wang, Gaohong, Huang, Kaiyao. Rapid construction and screening of artificial microRNA systems in Chlamydomonas reinhardtii. PLANT JOURNAL[J]. 2014, 79(6): 1052-1064, http://ir.scbg.ac.cn/handle/344003/17975.
[42] Long, Huan, Huang, Kaiyao, Marshall, WF. Analysis of Flagellar Protein Ubiquitination. CILIA, PT A[J]. 2013, 524: 59-73, https://www.webofscience.com/wos/woscc/full-record/WOS:000318253200005.
[43] Wood, Christopher R, Huang, Kaiyao, Diener, Dennis R, Rosenbaum, Joel L. The Cilium Secretes Bioactive Ectosomes. CURRENT BIOLOGY[J]. 2013, 23(10): 906-911, http://dx.doi.org/10.1016/j.cub.2013.04.019.
[44] Kaiyao Huang, CheChia Tsao. Importin-β2: a key to two gates?. 蛋白质与细胞:英文版[J]. 2010, 1(9): 791-792, http://lib.cqvip.com/Qikan/Article/Detail?id=7105125444.
[45] Huang, Kaiyao, Diener, Dennis R, Mitchell, Aaron, Pazour, Gregory J, Witman, George B, Rosenbaum, Joel L. Function and dynamics of PKD2 in Chlamydomonas reinhardtii flagella. JOURNAL OF CELL BIOLOGY[J]. 2007, 179(3): 501-514, https://www.webofscience.com/wos/woscc/full-record/WOS:000250705200015.
[46] Im, ChungSoon, Eberhard, Stephan, Huang, Kaiyao, Beck, Christoph F, Grossman, Arthur R. Phototropin involvement in the expression of genes encoding chlorophyll and carotenoid biosynthesis enzymes and LHC apoproteins in Chlamydomonas reinhardtii. PLANT JOURNAL[J]. 2006, 48(1): 1-16, https://www.webofscience.com/wos/woscc/full-record/WOS:000240440400001.
[47] Kaiyao Huang. Synthesis of the photorespiratory key enzyme glyoxylate aminotransferase in C. reinhardtii modulated by light regime and cytokinin. Physiol. Plant.. 2006, [48] Kaiyao Huang. Phototropin is the bluelight receptor that controls changes in chemotaxis during the initial phase of the sexual life cycle in Chlamydomonas. Planta. 2004, [49] Huang, KY, Kunkel, T, Beck, CF. Localization of the blue-light receptor phototropin to the flagella of the green alga Chlamydomonas reinhardtii. MOLECULAR BIOLOGY OF THE CELL[J]. 2004, 15(8): 3605-3614, https://www.webofscience.com/wos/woscc/full-record/WOS:000222951500010.
[50] Kaiyao Huang, Christoph F Beck. Phototropin is the blue-light receptor that controls multiple steps in the sexual life cycle of the green alga Chlamydomonas reinhardtii. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA. 2003, 100(10): 6269-6274, [51] Huang, KY, Merkle, T, Beck, CF. Isolation and characterization of a Chlamydomonas gene that encodes a putative blue-light photoreceptor of the phototropin family. PHYSIOLOGIA PLANTARUM[J]. 2002, 115(4): 613-622, https://www.webofscience.com/wos/woscc/full-record/WOS:000176802200016.
[52] Kaiyao Huang. Effects of salt stress on the cell structure and N2 fixation in a Blue-Green alga Anabaena Cylindrical. Chinese Bulletin of Botany. 1998, [53] 郭厚良, 黄开耀, 易平, 金传荫. KCl诱导柱胞鱼腥藻形成液泡. 水生生物学报[J]. 1998, 22(2): 198-199, http://lib.cqvip.com/Qikan/Article/Detail?id=3174418.
[54] Kaiyao Huang. Vacuole formation by KCI induction in a Blue-Green alga Anabaena Cylindrical. ACTA HYDROBILOGICA SINICA. 1998, [55] 易平, 郭厚良, 黄开耀. 盐胁迫对柱胞鱼腥藻细胞结构和固氮作用的影响. 植物学通报[J]. 1998, 15(3): 54-, http://lib.cqvip.com/Qikan/Article/Detail?id=3174283.
[56] 黄开耀, 郭厚良. 蓝藻突变体研究综述. 武汉植物学研究[J]. 1998, 16(3): 263-, http://lib.cqvip.com/Qikan/Article/Detail?id=3204748.
[57] Huang Kaiyao, Diener Dennis R., Rosenbaum Joel L.. The ubiquitin conjugation system is involved in the disassembly of cilia and flagella. THE JOURNAL OF CELL BIOLOGY. 186(4): 601-613, https://www.webofscience.com/wos/woscc/full-record/WOS:000269439200015.
[58] Baolong Zhang, Yuxin Tang, Fei Yu, Zhao Peng, Sheng Yao, Xuan Deng, Huan Long, Xun Wang, Kaiyao Huang. Translatomics and physiological analyses of the detoxification mechanism of green alga Chlamydomonas reinhardtii to cadmium toxicity. JOURNAL OF HAZARDOUS MATERIALS. http://dx.doi.org/10.1016/j.jhazmat.2023.130990.

   

（ 1 ） 油滴及纤毛的组装和去组装, 主持, 市地级, 2011-01--2014-01
（ 2 ） 鞭毛/鞭毛膜的形成、代谢及在细胞间信号传递过程中的作用, 主持, 国家级, 2012-01--2015-12
（ 3 ） ****择优支持, 主持, 部委级, 2012-01--2014-12
（ 4 ） 纤毛病的斑马鱼模型的建立, 参与, 国家级, 2002-01--2015-12
（ 5 ） IFT运输纤毛蛋白的分子机理, 主持, 国家级, 2014-01--2017-12
（ 6 ） 能源微藻油脂积累的分子机理及其应用研究, 主持, 院级, 2014-01--2016-12
（ 7 ） 斑马鱼囊泡运输蛋白发育调控研究, 主持, 国家级, 2013-01--2015-12
（ 8 ） 衣藻突变体库的构建和筛选技术, 主持, 院级, 2015-10--2020-10
（ 9 ） 微藻生物固碳关键技术, 主持, 国家级, 2014-02--2018-12
（ 10 ） 溶酶体对发育信号稳态调节的机制, 主持, 部委级, 2013-05--2016-04
（ 11 ） N-糖基化修饰在纤毛膜蛋白的运输及功能调节中的作用, 主持, 国家级, 2017-01--2021-12
（ 12 ） 功能性饲料产品研发—生产富含omega-3多不饱和脂肪酸的畜禽产品的藻类饲料研制, 主持, 省级, 2016-01--2020-12
（ 13 ） 药用单细胞真核微藻工程株的设计构建, 参与, 国家级, 2019-01--2023-12
（ 14 ） 雪藻驱动极地碳循环及适应低温和高辐射的机制, 主持, 国家级, 2019-01--2022-12

已指导学生

曾辉  硕士研究生  085238-生物工程  

缪荣丽  博士研究生  071004-水生生物学  

王亚丽  硕士研究生  071004-水生生物学  

王启宇  博士研究生  071007-遗传学  

程希  硕士研究生  071004-水生生物学  

陈天兵  博士研究生  071007-遗传学  

张璠  博士研究生  071007-遗传学  

吕波  博士研究生  071007-遗传学  

彭钊  博士研究生  071007-遗传学  

赵丽娟  博士研究生  071007-遗传学  

现指导学生

杨会慧  博士研究生  071007-遗传学  

段光前  硕士研究生  071007-遗传学  

徐南南  博士研究生  071007-遗传学  

林轶文  硕士研究生  071007-遗传学  

张宝龙  博士研究生  071007-遗传学  

许瑞  硕士研究生  071007-遗传学  

叶莲  博士研究生  071007-遗传学