071010-生物化学与分子生物学

RNA干扰相关蛋白与RNA复合物的结构与功能研究
结构生物学, RNA
RNA介导的抗病毒作用机理

2002-02--2005-01   中国科技大学   博士
1996-09--1999-07   中国农业科学院   硕士学位
1992-09--1996-07   武汉大学   学士学位

   

2011-04~现在, 中国科学院生物物理研究所, 研究员
2006-09~2011-02,memorial Sloan-kettering Cancer Center, Senior Research Scientist
2006-02~2006-08,美国佐治亚大学, 访问学者
2005-03~2006-02,中国科学院生物物理研究所, 助理研究员
1999-08~2001-09,安徽安科生物技术公司, 研究实习员

2014-06-05-今,Non-coding RNA杂志, 编委

molecular biology

   

（1） 中科院****终期优秀, 院级, 2015
（2） 中科院优秀导师奖, 一等奖, 研究所（学校）, 2015
（3） 细胞出版社中国年度论文奖, 其他, 2015

   

[1] 王艳丽. 针对II型CRISPR-Cas系统的Acr基因的发现及Acr蛋白多样化的抑制机制. 中国科学:生命科学[J]. 2023, https://engine.scichina.com/doi/pdf/89B0BDB5F8CC46CE9831C0D947D4CE9B.
[2] Li, Hui, Wang, Jinlon, Kuan, Tung Ariel, Tang, Bozeng, Feng, Li, Wang, Jiuyu, Cheng, Zhi, Sklenar, Jan, Hulin, Michelle, Li, Yufei, Zhai, Yi, Hou, Yingnan, Menke, Frank L H, Wang, Yanli, Ma, Wenbo. Pathogen protein modularity enables elaborate mimicry of a host phosphatase. CELL[J]. 2023, 186(15): 3196-+, http://dx.doi.org/10.1016/j.cell.2023.05.049.
[3] Wei Sun, Xiaolong Zhao, Jinlong Wang, Xiaoqi Yang, Zhi Cheng, Shuo Liu, Jiuyu Wang, Gang Sheng, Yanli Wang. Anti-CRISPR AcrIIC5 is a dsDNA mimic that inhibits type II-C Cas9 effectors by blocking PAM recognition. NUCLEIC ACIDS RESEARCH[J]. 2023, 51(4): 1984-1995, https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9976890/.
[4] Yanli Wang. AcrIIC4 inhibits type II-C Cas9 by preventing R-loop formation. Proceedings of the National Academy of Sciences of the United States of America[J]. 2023, 120(31): e2303675120-, https://www.pnas.org/doi/10.1073/pnas.2303675120?url_ver=Z39.88-2003&rfr_id=ori:rid:crossref.org&rfr_dat=cr_pub%20%200pubmed.
[5] Chen Peng, Sun Wei, Cheng Zhi, Yang Jing, Wang Min, Wang JiuYu, Chen HuiQing, Liu Liang, Wang YanLi. Structural Study on Anti-CRISPR Protein AcrVA2. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS[J]. 2021, 48(1): 77-87, http://sciencechina.cn/gw.jsp?action=detail.jsp&internal_id=6890297&detailType=1.
[6] 陈鹏, 孙伟, 程志, 杨晶, 王敏, 王久宇, 陈慧卿, 刘亮, 王艳丽. Anti-CRISPR蛋白AcrVA2的结构生物学研究. 生物化学与生物物理进展[J]. 2021, 48(1): 77-87, http://lib.cqvip.com/Qikan/Article/Detail?id=7103981232.
[7] 王康康, 王艳丽. 以Cas X为例简述新型CRISPR-Cas系统的基本属性和研究方法. 微生物学通报[J]. 2021, 48(4): 1227-1238, http://lib.cqvip.com/Qikan/Article/Detail?id=7104632286.
[8] 尤李兰, 孙伟, 杨晓琪, 王艳丽. 诺贝尔化学奖授予CRISPR-Cas9基因编辑研究. 生物化学与生物物理进展[J]. 2020, 47(11): 1119-1126, http://lib.cqvip.com/Qikan/Article/Detail?id=7103402811.
[9] You LiLan, Sun Wei, Yang XiaoQi, Wang YanLi. Chemistry Nobel Honors CRISPR-Cas9. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS[J]. 2020, 47(11): 1119-1126, http://dx.doi.org/10.16476/j.pibb.2020.0395.
[10] Mathony, Jan, Harteveld, Zander, Schmelas, Carolin, zu Belzen, Julius Upmeier, Aschenbrenner, Sabine, Sun, Wei, Hoffmann, Mareike D, Stengl, Christina, Scheck, Andreas, Georgeon, Sandrine, Rosset, Stephane, Wang, Yanli, Grimm, Dirk, Eils, Roland, Correia, Bruno E, Niopek, Dominik. Computational design of anti-CRISPR proteins with improved inhibition potency. NATURE CHEMICAL BIOLOGY[J]. 2020, 16(7): 725-+, http://dx.doi.org/10.1038/s41589-020-0518-9.
[11] Xue Huang, Wei Sun, Zhi Cheng, Minxuan Chen, Xueyan Li, Jiuyu Wang, Gang Sheng, Weimin Gong, Yanli Wang. Structural basis for two metal-ion catalysis of DNA cleavage by Cas12i2. NATURE COMMUNICATIONS[J]. 2020, 11(1): http://dx.doi.org/10.1038/s41467-020-19072-6.
[12] Yang, Jing, Li, Jiazhi, Wang, Jiuyu, Sheng, Gang, Wang, Min, Zhao, Hongtu, Yang, Yanhua, Wang, Yanli. Crystal structure of Cas1 in complex with branched DNA. SCIENCE CHINA-LIFE SCIENCES[J]. 2020, 63(4): 516-528, http://lib.cqvip.com/Qikan/Article/Detail?id=7101497804.
[13] 陈鹏, 孙伟, 程志, 杨晶, 王敏, 王久宇, 陈慧卿, 刘亮, 王艳丽. Anti-CRISPR蛋白AcrVA2的结构生物学研究. 生物化学与生物物理进展. 2020, 77-87, https://nxgp.cnki.net/kcms/detail?v=3uoqIhG8C46NmWw7YpEsKMypi3qVj28LEUDxQXHYyS3fbyEOrIfBzH9VRyF39XUyNdXdeQeJeAwqu5gEBeFLwXPlfq81zcLg&uniplatform=NZKPT.
[14] You, Lilan, Ma, Jun, Wang, Jiuyu, Artamonova, Daria, Wang, Min, Liu, Liang, Xiang, Hua, Severinov, Konstantin, Zhang, Xinzheng, Wang, Yanli. Structure Studies of the CRISPR-Csm Complex Reveal Mechanism of Co-transcriptional Interference. CELL[J]. 2019, 176(1-2): 239-+, http://dx.doi.org/10.1016/j.cell.2018.10.052.
[15] Sun, Wei, Yang, Jing, Cheng, Zhi, Amrani, Nadia, Liu, Chao, Wang, Kangkang, Ibraheim, Raed, Edraki, Alireza, Huang, Xue, Wang, Min, Wang, Jiuyu, Liu, Liang, Sheng, Gang, Yang, Yanhua, Lou, Jizhong, Sontheimer, Erik J, Wang, Yanli. Structures of Neisseria meningitidis Cas9 Complexes in Catalytically Poised and Anti-CRISPR-Inhibited States. MOLECULAR CELL[J]. 2019, 76(6): 938-+, https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6934045/.
[16] Chang, Lei, Sheng, Gang, Zhang, Yiwen, Shao, Shipeng, Wang, Yanli, Sun, Yujie. AgoFISH: cost-effective in situ labelling of genomic loci based on DNA-guided dTtAgo protein. NANOSCALE HORIZONS[J]. 2019, 4(4): 918-923, [17] Annoj Thavalingam, Zhi Cheng, Bianca Garcia, Xue Huang, Megha Shah, Wei Sun, Min Wang, Lucas Harrington, Sungwon Hwang, Yurima HidalgoReyes, Erik J. Sontheimer, Jennifer Doudna, Alan R. Davidson, Trevor F. Moraes, Yanli Wang, Karen L. Maxwell. Inhibition of CRISPR-Cas9 ribonucleoprotein complex assembly by anti-CRISPR AcrIIC2. NATURE COMMUNICATIONS[J]. 2019, 10(1): 1-11, [18] WANG Yanli. Zoom in on DNA Cleavage by Cas9. 中国科学院院刊:英文版[J]. 2019, 33(4): 247-248, http://lib.cqvip.com/Qikan/Article/Detail?id=7101680921.
[19] Liu, Liang, Yin, Maolu, Wang, Min, Wang, Yanli. Phage AcrIIA2 DNA Mimicry: Structural Basis of the CRISPR and Anti-CRISPR Arms Race. MOLECULAR CELL[J]. 2019, 73(3): 611-+, http://dx.doi.org/10.1016/j.molcel.2018.11.011.
[20] Lei, Jinping, Sheng, Gang, Cheung, Peter PakHang, Wang, Shenglong, Li, Yu, Gao, Xin, Zhang, Yingkai, Wang, Yanli, Huang, Xuhui. Two symmetric arginine residues play distinct roles in Thermus thermophilus Argonaute DNA guide strand-mediated DNA target cleavage. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA[J]. 2019, 116(3): 845-853, [21] Cao, Yuanwei, Sun, Wen, Wang, Jinfeng, Sheng, Gang, Xiang, Guanghai, Zhang, Tongtong, Shi, Wenyu, Li, Chun, Wang, Yanli, Zhao, Fangqing, Wang, Haoyi. Argonaute proteins from human gastrointestinal bacteria catalyze DNA-guided cleavage of single- and double-stranded DNA at 37 degrees C. CELL DISCOVERYnull. 2019, 5: http://dx.doi.org/10.1038/s41421-019-0105-y.
[22] Zhao, Xiao, Liu, Liang, Liang, Jiayan, Cheng, Keman, Wang, Yongwei, Li, Xueyan, Shi, Jian, Wang, Yanli, Nie, Guangjun. A CRISPR-Cas13a system for efficient and specific therapeutic targeting of mutant KRAS for pancreatic cancer treatment. CANCER LETTERS[J]. 2018, 431: 171-181, http://dx.doi.org/10.1016/j.canlet.2018.05.042.
[23] Liang, Liu, Xueyan, Li, Jun, Ma, Zongqiang, Li, Lilan, You, Jiuyu, Wang, Min, Wang, 章新政, 王艳丽. RNA介导切割RNA的Cas13a复合物分子结构. 科学新闻[J]. 2018, 45-45, http://lib.cqvip.com/Qikan/Article/Detail?id=675303202.
[24] Liang, Liu, Xueyan, Li, Jiuyu, Wang, Min, Wang, Peng, Chen, Maolu, Yin, Jiazhi, Li, Gang, Sheng, 王艳丽. C2c2依赖两个远离的活性中心发挥RNA酶活性. 科学新闻[J]. 2018, 33-33, http://lib.cqvip.com/Qikan/Article/Detail?id=675303185.
[25] Liang, Liu, Peng, Chen, Min, Wang, Xueyan, Li, Jiuyu, Wang, Maolu, Yin, 王艳丽. C2c1-sgRNA复合物结构揭示RNA介导的C2c1切割DNA的机制. 科学新闻[J]. 2018, 83-83, http://lib.cqvip.com/Qikan/Article/Detail?id=675303270.
[26] Song, Guangtao, Chen, Hui, Sheng, Gang, Wang, Yanli, Lou, Jizhong. Argonaute Facilitates the Lateral Diffusion of the Guide along Its Target and Prevents the Guide from Being Pushed Away by the Ribosome. BIOCHEMISTRY[J]. 2018, 57(15): 2179-2183, https://www.webofscience.com/wos/woscc/full-record/WOS:000430514800002.
[27] Liu, Liang, Li, Xueyan, Wang, Jiuyu, Wang, Min, Chen, Peng, Yin, Maolu, Li, Jiazhi, Sheng, Gang, Wang, Yanli. Two Distant Catalytic Sites Are Responsible for C2c2 RNase Activities. CELL[J]. 2017, 168(1-2): 121-+, http://dx.doi.org/10.1016/j.cell.2016.12.031.
[28] Maolu Yin, Jiuyu Wang, Min Wang, Xinmei Li, Mo Zhang, Qiang Wu, Yanli Wang. Molecular mechanism of directional CTCF recognition of a diverse range of genomic sites. 细胞研究英文版[J]. 2017, 27(11): 1365-1377, http://lib.cqvip.com/Qikan/Article/Detail?id=673788627.
[29] Sheng, Gang, Gogakos, Tasos, Wang, Jiuyu, Zhao, Hongtu, Serganov, Artem, Juranek, Stefan, Tuschl, Thomas, Patel, Dinshaw J, Wang, Yanli. Structure/cleavage-based insights into helical perturbations at bulge sites within T. thermophilus Argonaute silencing complexes. NUCLEIC ACIDS RESEARCH[J]. 2017, 45(15): 9149-9163, http://dx.doi.org/10.1093/nar/gkx547.
[30] Zhu, Lizhe, Jiang, Hanlun, Sheong, Fu Kit, Cui, Xuefeng, Wang, Yanli, Gao, Xin, Huang, Xuhui. Understanding the core of RNA interference: The dynamic aspects of Argonaute-mediated processes. PROGRESS IN BIOPHYSICS & MOLECULAR BIOLOGY[J]. 2017, 128: 39-46, http://dx.doi.org/10.1016/j.pbiomolbio.2016.09.008.
[31] Yin, Maolu, Wang, Jiuyu, Wang, Min, Li, Xinmei, Zhang, Mo, Wu, Qiang, Wang, Yanli. Molecular mechanism of directional CTCF recognition of a diverse range of genomic sites. CELL RESEARCH[J]. 2017, 27(11): 1365-1377, http://lib.cqvip.com/Qikan/Article/Detail?id=673788627.
[32] Liu, Liang, Li, Xueyan, Ma, Jun, Li, Zongqiang, You, Lilan, Wang, Jiuyu, Wang, Min, Zhang, Xinzheng, Wang, Yanli. The Molecular Architecture for RNA-Guided RNA Cleavage by Cas13a. CELL[J]. 2017, 170(4): 714-+, http://dx.doi.org/10.1016/j.cell.2017.06.050.
[33] Liu, Liang, Chen, Peng, Wang, Min, Li, Xueyan, Wang, Jiuyu, Yin, Maolu, Wang, Yanli. C2c1-sgRNA Complex Structure Reveals RNA-Guided DNA Cleavage Mechanism. MOLECULAR CELL[J]. 2017, 65(2): 310-322, http://dx.doi.org/10.1016/j.molcel.2016.11.040.
[34] Swarts, Daan C, Szczepaniak, Malwina, Sheng, Gang, Chandradoss, Stanley D, Zhu, Yifan, Timmers, Elizabeth M, Zhang, Yong, Zhao, Hongtu, Lou, Jizhong, Wang, Yanli, Joo, Chirlmin, van der Oost, John. Autonomous Generation and Loading of DNA Guides by Bacterial Argonaute. MOLECULAR CELL[J]. 2017, 65(6): 985-+, http://dx.doi.org/10.1016/j.molcel.2017.01.033.
[35] 王久宇, 王艳丽. CRISPR-Cas系统PAM依赖的新间隔序列获取的结构及分子机制. 中国科学基金[J]. 2016, 30(5): 443-446, http://sciencechina.cn/gw.jsp?action=detail.jsp&internal_id=5803188&detailType=1.
[36] 赵宏图, 王艳丽. CRISPR-Cas系统中crRNA产生及干扰机制的研究. 生命科学[J]. 2016, 28(5): 584-591, http://sciencechina.cn/gw.jsp?action=detail.jsp&internal_id=5740212&detailType=1.
[37] Zhu, Lizhe, Jiang, Hanlun, Sheong, Fu Kit, Cui, Xuefeng, Gao, Xin, Wang, Yanli, Huang, Xuhui. A Flexible Domain-Domain Hinge Promotes an Induced-fit Dominant Mechanism for the Loading of Guide-DNA into Argonaute Protein in Thermus thermophilus. JOURNAL OF PHYSICAL CHEMISTRY B[J]. 2016, 120(10): 2709-2720, https://www.webofscience.com/wos/woscc/full-record/WOS:000372562000007.
[38] Wang, Jiuyu, Ma, Jun, Cheng, Zhi, Meng, Xu, You, Lilan, Wang, Min, Zhang, Xinzheng, Wang, Yanli. A CRISPR evolutionary arms race: structural insights into viral anti-CRISPR/Cas responses. CELL RESEARCH[J]. 2016, 26(10): 1165-1168, http://lib.cqvip.com/Qikan/Article/Detail?id=670592062.
[39] Yanli Wang. Structural and Mechanistic Basis of PAM-Dependent Spacer Acquisition in CRISPR-Cas System. Cell. 2015, [40] Huang, Ye, Tian, Miao, Zhang, Yichao, Sheng, Gang, Chen, Zhuo, Ma, Yuan, Chen, Yue, Peng, Yihong, Zhao, YiLei, Wang, Yanli, Zhang, Lihe, Yang, Zhenjun. D-Isonucleotide (isoNA) incorporation around cleavage site of passenger strand promotes the vibration of Ago2-PAZ domain and enhances in vitro potency of siRNA. ORGANIC & BIOMOLECULAR CHEMISTRY[J]. 2015, 13(44): 10825-10833, http://dx.doi.org/10.1039/c5ob01119a.
[41] Wang, Jiuyu, Li, Jiazhi, Zhao, Hongtu, Sheng, Gang, Wang, Min, Yin, Maolu, Wang, Yanli. Structural and Mechanistic Basis of PAM-Dependent Spacer Acquisition in CRISPR-Cas Systems. CELL[J]. 2015, 163(4): 840-853, http://dx.doi.org/10.1016/j.cell.2015.10.008.
[42] Sheng, Gang, Zhao, Hongtu, Wang, Jiuyu, Rao, Yu, Tian, Wenwen, Swarts, Daan C, van der Oost, John, Patel, Dinshaw J, Wang, Yanli. Structure-based cleavage mechanism of Thermus thermophilus Argonaute DNA guide strand-mediated DNA target cleavage. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA[J]. 2014, 111(2): 652-657, http://dx.doi.org/10.1073/pnas.1321032111.
[43] Zhao, Hongtu, Sheng, Gang, Wang, Jiuyu, Wang, Min, Bunkoczi, Gabor, Gong, Weimin, Wei, Zhiyi, Wang, Yanli. Crystal structure of the RNA-guided immune surveillance Cascade complex in Escherichia coli. NATURE[J]. 2014, 515(7525): 147-+, http://dx.doi.org/10.1038/nature13733.
[44] Swarts, Daan C, Makarova, Kira, Wang, Yanli, Nakanishi, Kotaro, Ketting, Rene F, Koonin, Eugene V, Patel, Dinshaw J, van der Oost, John. The evolutionary journey of Argonaute proteins. NATURE STRUCTURAL & MOLECULAR BIOLOGYnull. 2014, 21(9): 743-753, https://www.webofscience.com/wos/woscc/full-record/WOS:000341404200003.
[45] Swarts, Daan C, Jore, Matthijs M, Westra, Edze R, Zhu, Yifan, Janssen, Jorijn H, Snijders, Ambrosius P, Wang, Yanli, Patel, Dinshaw J, Berenguer, Jose, Brouns, Stan J J, van der Oost, John. DNA-guided DNA interference by a prokaryotic Argonaute. NATURE[J]. 2014, 507(7491): 258-+, http://dx.doi.org/10.1038/nature12971.
[46] Ruedel, Sabine, Wang, Yanli, Lenobel, Rene, Koerner, Roman, Hsiao, HeHsuan, Urlaub, Henning, Patel, Dinshaw, Meister, Gunter. Phosphorylation of human Argonaute proteins affects small RNA binding. NUCLEIC ACIDS RESEARCH[J]. 2011, 39(6): 2330-2343, http://dx.doi.org/10.1093/nar/gkq1032.
[47] Wang, Yanli, Ludwig, Janos, Schuberth, Christine, Goldeck, Marion, Schlee, Martin, Li, Haitao, Juranek, Stefan, Sheng, Gang, Micura, Ronald, Tuschl, Thomas, Hartmann, Gunther, Patel, Dinshaw J. Structural and functional insights into 5 '-ppp RNA pattern recognition by the innate immune receptor RIG-I. NATURE STRUCTURAL & MOLECULAR BIOLOGY[J]. 2010, 17(7): 781-U19, https://www.webofscience.com/wos/woscc/full-record/WOS:000279631500002.
[48] Wang, Yanli, Juranek, Stefan, Li, Haitao, Sheng, Gang, Wardle, Greg S, Tuschl, Thomas, Patel, Dinshaw J. Nucleation, propagation and cleavage of target RNAs in Ago silencing complexes. NATURE[J]. 2009, 461(7265): 754-U3, https://www.webofscience.com/wos/woscc/full-record/WOS:000270547500028.
[49] Liu, Rujuan, Xu, Hang, Wei, Zhiyi, Wang, Yanli, Lin, Yajing, Gong, Weimin. Crystal structure of human adenylate kinase 4 (L171P) suggests the role of hinge region in protein domain motion. BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS[J]. 2009, 379(1): 92-97, http://dx.doi.org/10.1016/j.bbrc.2008.12.012.
[50] Wang, Yanli, Juranek, Stefan, Li, Haitao, Sheng, Gang, Tuschl, Thomas, Patel, Dinshaw J. Structure of an argonaute silencing complex with a seed-containing guide DNA and target RNA duplex. NATURE[J]. 2008, 456(7224): 921-U72, https://www.webofscience.com/wos/woscc/full-record/WOS:000261768300038.
[51] Wang, Yanli, Sheng, Gang, Juranek, Stefan, Tuschl, Thomas, Patel, Dinshaw J. Structure of the guide-strand-containing argonaute silencing complex. NATURE[J]. 2008, 456(7219): 209-U34, https://www.webofscience.com/wos/woscc/full-record/WOS:000261039300033.
[52] Hou, Xiaowei, Wang, Yanli, Zhou, Zhongwei, Bao, Shilai, Lin, Yajing, Gong, Weimin. Crystal structure of SAM-dependent O-methyltransferase from pathogenic bacterium Leptospira interrogans. JOURNAL OF STRUCTURAL BIOLOGY[J]. 2007, 159(3): 523-528, http://dx.doi.org/10.1016/j.jsb.2007.04.007.
[53] Zhang, Cheng, Liu, Lin, Xu, Hang, Wei, Zhiyi, Wang, Yanli, Lin, Yajing, Gong, Weimin. Crystal structures of human IPP isomerase: New insights into the catalytic mechanism. JOURNAL OF MOLECULAR BIOLOGY[J]. 2007, 366(5): 1437-1446, http://dx.doi.org/10.1016/j.jmb.2006.10.092.

   

（ 1 ） 973干细胞导向项目, 参与, 国家级, 2011-01--2014-12
（ 2 ） ****, 主持, 部委级, 2011-06--2013-06
（ 3 ） crRNA介导的免疫系统的结构与功能研究, 主持, 国家级, 2015-01--2018-12
（ 4 ） RLR识别配体及免疫应答基因调控的结构基础与分子机制, 主持, 国家级, 2014-01--2018-12

已指导学生

田文文  02  19182  

陈琳  02  19183  

赵宏图  01  19183  

现指导学生

尹茂鲁  01  19183  

尤李兰  02  19183  

李佳智  01  19183  

陈鹏  02  19183  

李雪岩  02  19183  

陈敏轩   02  19182  

程志   02  19183  

李新美  01  19183