General
Name:
LI, Wei
Subject:
Developmental Biology
Tel/Fax:
+86-10-64807529  / 
E-mail:
leways@gmail.com, leways@ioz.ac.cn
Address:
The State Key Laboratory of Stem cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, 1 Beichen West Road, Chaoyang District, Beijing 100101, P.R.China


Research Areas

 His research focuses on the mechanisms of protein modification and their roles in reproduction and aging. His group systematically studied on the mechanisms of ubiquitin and ubiquititn-like modifications on spermiogenesis, meiosis and somatic-germ cell interaction with multiple approaches. Recently, they have made a series of accomplishments on “the functional roles of ubiquitin and ubiquititn-like modifications in gametogenesis”, including: 1) They proposed and validated the acrosome’s autolysosome origination hypothesis. 2) They found that autophagy participates in extra cytoplasm removal in the germ cells and ectoplasmic specialization assembly in Sertoli cells. 3) They elucidate the mechanism of N-end rule pathway during meiosis. In addition, they investigate the molecular mechanisms underlying some E2 and E3 which are related to testosterone synthesis. Their research not only provides new insight into the regulation of gametogenesis at the posttranslational modification level, but also might provide new way to the diagnosis and even the therapy of human sterility and reproductive related disease.


Education

09/1998-07/2003, Institute of Cell Biology, Lanzhou University, Ph.D.
09/1994-07/1998, School of life science, Lanzhou University, B.S.


Experience

05/2009-Present PI, State Key Laboratory of Stem cell and Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, 100101, P.R. China
06/2005-05/2009 Postdoctoral visiting fellow, National Institute of Diabetes and Digestive and Kidney Diseases, NIH, MD 20892
08/2003-06/2005 Postdoctoral research fellow, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing, 100086, P.R. China


Teaching Experience

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Publications

1.        Liu C, Li W*, The molecular mechanism underlying acrosome biogenesis. Spermatogenesis: Molecular Mechanisms, Regulation and Biological Perspectives. 2016. https://www.novapublishers.com/catalog/product_info.php?products_id=57504.


Papers
  1. Xu Z, Song Z, Li G, Tu H, Liu W, Liu Y, Wang P, Wang Y, Cui X, Liu C, Shang Y, de Rooij DG, Gao F, Li W*. H2B ubiquitination regulates meiotic recombination by promoting chromatin relaxation. Nucleic Acids Res. 2016 Jul 18. pii: gkw652. [Epub ahead of print]
  2. Liu Y, Liu C, Dong W*, Li W*. Physiological functions and clinical implications of the N-end rule pathway.Front Med. 2016 Aug 5. [Epub ahead of print] Review.
  3. Li S*, Liu C, Gu L, Wang L, Shang Y, Liu Q, Wan J, Shi J, Wang F, Xu Z, Ji G*, Li W*. Autophagy protects cardiomyocytes from the myocardial ischaemia-reperfusion injury through the clearance of CLP36. Open Biol. 2016 Aug;6(8). pii: 160177. doi: 10.1098/rsob.160177.
  4. Shang YL, Wang HN, Jia PF, Zhao HC, Liu C, Liu WX, Song ZH, Xu ZL,Yang L, Wang YF, Li W*. Autophagy regulates spermatid differentiation via degradation of PDLIM1. Autophagy. 2016 Jun 16:1-18. [Epub ahead of print].
  5. Gao F, Shang Y, Liu W, Li W*. The linkage specificity determination of Ube2g2-gp78 mediated polyubiquitination. Biochem Biophys Res Commun. 2016 May 13;473(4):1139-43. 
  6. Liu YJ, Liu C, Chang ZN, Wadas B, Brower CS, Song ZH, Xu ZL, Shang YL, Liu WX, Wang LN, Dong W*, Varshavsky A*, Hu RG*, and Li W*, Degradation of the Separase-cleaved Rec8, a Meiotic Cohesin Subunit, by the N-end Rule Pathway. J Biol Chem. 2016 Apr 1;291(14):7426-38.
  7. Wen FP, Guo YS, Hu Y, Wang YT, Wang Q, Yu HY, Tang CM, Yang J, Zhou T, Sha JH, Guo XJ*, Li W*.Distinct temporal requirements for autophagy and proteasome in yeast meiosis. Autophagy. 2016 Apr 2;12(4):671-88.
  8. Liu C，Wang HN, Shang YL, Liu WX, Song ZH, Zhao HC, Wang LN, Jia PF, Gao FY,  Xu ZL, Yang L, Gao F,Li W*. Autophagy is required for ectoplasmic specialization assembly in Sertoli cells. Autophagy. 2016May 3;12(5):814-32.
  9. Klionsky DJ，...Li W... Zughaier SM. Guidelines for the use and interpretation of assays for monitoring autophagy (3rd edition). Autophagy. 2016 Jan 2;12(1):1-222.
  10. Liu C, Li W*, The molecular mechanism underlying acrosome biogenesis. Spermatogenesis: Molecular Mechanisms, Regulation and Biological Perspectives. 2016 , pp. 1-30 (Nova Science Publishers).
  11. Liu M, Cao GM, Zhang YM, Qu JP, Li W, Wan XR, Li YX, Zhang ZB*, Wang YL*,  Gao F*. Changes in the morphology and protein expression of germ cells and Sertoli cells in plateau pikas testes during non-breeding season. Sci Rep. 2016 Mar 4;6:22697. doi: 10.1038/srep22697.
  12. Zhang P, He Q, Chen D, Liu W, Wang L, Zhang C, Ma D, Li W, Liu B, Liu F. G protein-coupled receptor 183 facilitates endothelial-to-hematopoietic transition via Notch1 inhibition. Cell Res. 2015 Oct;25(10):1093-107.
  13. Wang Q, Liu C, Tang C, Guo H, Liu Y, Wang L, Zhao H, Shang Y, Wen Y, Lin Y, Zhou T, Zhou Z, Dong W, Hu Z, Guo X, Sha J, Li W*. Yeast model identifies ENTPD6 as a potential non-obstructive azoospermia pathogenic gene. Sci Rep. 2015 Jul 8;5:11762. doi: 10.1038/srep11762.
  14. Song ZH, Yu HY, Wang P, Mao GK, Liu WX, Li MN, Wang HN, Shang YL, Liu C, Xu ZL, Sun QY, Li W*, Germ cell specific Atg7 knockout results in primary ovarian insufficiency in female mice. Cell Death Dis.  2015 Jan 15;6:e1589. doi: 10.1038/cddis.2014.559.
  15. Liu WX, Shang YL, Li W*. gp78 G2BR and CUE domains cooperatively elongate polyubiquitin chains from the distal end. Sci. Rep. 2014 Nov 20;4:7138. doi: 10.1038/srep07138.  
  16. Wang HN, Wan HF, Li XX, Liu WX, Chen Q, Wang YQ, Yang L, Duan EK, Tang HM, Zhang XJ, Zhao XY, Gao F*, Li W*. Atg7 is required for acrosome biogenesis during spermatogenesis in mice. Cell Res. 2014 Jul;24(7):852-69.
  17. Li Y, Wu H, Wu W, Zhuo W, Liu W, Zhang Y, Cheng M, Chen YG, Gao N, Yu H, Wang L*, Li W*, Yang M*. Structural insights into the TRIM family of ubiquitin E3 ligases. Cell Res. 2014 Jun; 24(6):762-5.
  18. Chen M, Wang X, Wang Y, Zhang L, Xu B, Lv L, Cui X, Li W*, Gao F*. Wt1 is involved in leydig cell steroid hormone biosynthesis by regulating paracrine factor expression in mice. Biol Reprod. 2014 Apr 3;90(4):71, 1-9
  19. Yan L, Liu WX, Zhang HH, Liu C, Shang YL, Ye YH, Zhang XD*, Li W*, Ube2g2-gp78-mediated Herp polyubiquitination is involved in ER stress recovery. J Cell Sci. 2014 Apr 1;127: 1417-27.
  20. Liu WX, Shang YL, Zeng Y, Liu C, Li YC, Zhai LH, Wang P, Lou JZ, Xu P, Ye Y*, Li W*. Dimeric Ube2g2 simultaneously engages donor and acceptor ubiquitins to form Lys48-linked ubiquitin chains. EMBO J.2014 Jan 1;33(1):46-61.
  21. Liu Z, Ma L, Wen ZS, Hu Z, Wu FQ, Li W, Liu J, Zhou GB. Cancerous inhibitor of PP2A is targeted by natural compound celastrol for degradation in non-small-cell lung cancer. Carcinogenesis. 2014 Apr;35(4):905-14
  22. Qian MX, Pang Y, Liu CH, Haratake K, Du BY, Ji DY, Wang GF, Zhu QQ, Song W, Yu Y, Zhang XX, Huang HT, Miao S, Chen LB, Zhang ZH, Liang YN, Liu S, Cha H, Yang D, Zhai Y, Komatsu T, Tsuruta F, Li H, Cao C,Li W, Li GH, Cheng Y, Chiba T, Wang L, Goldberg AL, Shen Y, Qiu XB. Acetylation-mediated proteasomal degradation of core histones during DNA repair and spermatogenesis. Cell. 2013 May 23;153 (5):1012-24.
  23. Cui L, Li W*. Role of ubiquitination in meiotic recombination repair. Sci China Life Sci. 2010，53 (4): 447-454.
  24. Li W*, Tu D*, Li LY, Wollert T, Ghirlando R, Brunger AT, Ye Y. Mechanism of ubiquitin chain assembly on the active site cysteine of the ubiquitin conjugating enzyme Ube2g2. Proc Natl Acad Sci U S A. 2009 Mar 10; 106(10):3722-7.
  25. Li W and Ye Y (2008) Polyubiquitin chains: functions, structures, and mechanisms. Cell Mol Life Sci2008 Apr 26. 65(15): 2397-406
  26. Li W, Tu D, Brunger A, Ye Y. (2007) An ubiquitin ligase transfers preformed polyqubiquitin chains from a conjugating enzyme to substrate. Nature 446(7133):333-7.
  27. Tu D, Li W, Ye Y, Brunger A. Structure and function of the yeast U-box-containing ubiquitin ligase Ufd2p.Proc Natl Acad Sci U S A. 2007 104(40):15599-606.
  28. Li W*, Sun L*, Liang Q*, Wang J, Mo W, Zhou B. Yeast AMID homologue Ndi1p displays respiration-restricted apoptotic activity and is involved in chronological aging. Mol Biol Cell. 2006 Apr;17(4):1802-11.
  29. Li W, Mo W, Shen D, Sun L, Wang J, Lu S, Gitschier JM, Zhou B. Yeast model uncovers dual roles of mitochondria in action of artemisinin. PLoS Genet. 2005 1(3):e36 


Research Interests

1) Posttranslational modification regulation of gametogenesis, especially for meiosis.
2) The functional role of histone modifications in meiosis, stem cell reprogramming, self-renew and differentiation.
3) Posttranslational regulation basis of reproductive and aging related diseases.


Students

已指导学生

赵小红  02  19181  

闫龙  01  19181  

于海燕  02  19181  

温福平  01  19181  

王红娜  01  19181  

王瑗婷  02  19181  

唐超明  02  63228  

宋振华  01  19181  

王倩  02  19181  

刘超  01  19181  

现指导学生

徐志良  01  19181  

赵海超  01  19181  

尚永亮  01  19181  

韦华芳  02  19181  

温佳敏  02  19181  

王丽颖  02  19181  

时间  01  19181  

王芳  02  19181  

王丽娜  01  19181