Comparative immunology; Fish immunology

Ph.D., Institute of Hydrobiology, Chinese Academy of Sciences, China, 2005

M.Sc., HUAZHONG Agriculture University, China, 2001

   

2005-2007 Assistant Professor, Institute of Hydrobiology, Chinese Academy of Sciences

2007-2014 Associate Professor, Institute of Hydrobiology, Chinese Academy of Sciences

2008                        Research fellow, School of Biological Sciences, University of Aberdeen, Aberdeen AB24 2TZ

2009-2010               Visiting scholar, School of Biological Sciences, University of Aberdeen, Aberdeen AB24 2TZ

2015-present Professor, Institute of Hydrobiology, Chinese Academy of Sciences

Prevention and Control of Aquatic Animal Diseases and Immunology

   

1. Cao L, Wu XM, Nie P, Chang MX*. The negative regulation of piscine CD44c in viral and bacterial infection. Dev Comp Immunol. 2019 Jul;96:135-143.

2. Rahman K, Khan SU, Fahad S*, Chang MX*, Abbas A, Khan WU, Rahman L, Haq ZU, Nabi G, Khan D. Nano-biotechnology: a new approach to treat and prevent malaria. Int J Nanomedicine. 2019 Feb 21;14:1401-1410.

3. Zhang J, Guo H, Zhang F, Chen Q, Chang M*, Fang Q*. NS38 is required for aquareovirus replication via interaction with viral core proteins and host eIF3A. Virology. 2019 Feb 1;529:216-225.

4. Jiang X, Gao J, Xue Y, Qin Y, Li X, Sun Z, Xie H, Chang M, Nie P, Zou J*, Gao Q*. Identification and expression analysis of IL-4/13 receptors in grass carp Ctenopharyngodon idella. Fish Shellfish Immunol. 2019 Jan 8;87:254-264.

5. Wu XM, Chen WQ, Hu YW, Cao L, Nie P, Chang MX*. RIP2 Is a Critical Regulator for NLRs Signaling and MHC Antigen Presentation but Not for MAPK and PI3K/Akt Pathways. Front Immunol. 2018 Apr 10;9:726. doi: 10.3389/fimmu.2018.00726.

6. Cao L, Wu XM, Hu YW, Xue NN, Nie P, Chang MX*. The discrepancy function of NLRC5 isoforms in antiviral and antibacterial immune responses. Dev Comp Immunol. 2018 Jul;84:153-163.

7. Hu YW, Zhang J, Wu XM, Cao L, Nie P, Chang MX*. TANK-Binding Kinase 1 (TBK1) Isoforms Negatively Regulate Type I Interferon Induction by Inhibiting TBK1-IRF3 Interaction and IRF3 Phosphorylation. Front Immunol. 2018 Jan 30;9:84.

8. Zhen X, Zhou H, Ding W, Zhou B, Xu X, Perčulija V, Chen CJ, Chang MX, Choudhary MI, Ouyang S*. Structural basis of AimP signaling molecule recognition by AimR in Spbeta group of bacteriophages. Protein Cell. 2018 Nov 12. doi: 10.1007/s13238-018-0588-6.

9. Zhang B, Ye W, Ye Y, Zhou H, Saeed AFUH, Chen J, Lin J, Perčulija V, Chen Q, Chen CJ, Chang MX, Choudhary MI, Ouyang S*. Structural insights into Cas13b-guided CRISPR RNA maturation and recognition. Cell Res. 2018 Dec;28(12):1198-1201.

10. 任诗思，齐志涛，昌鸣先*. 大鲵短型肽聚糖识别蛋白PGRP-S的克隆及其功能研究.水生生物学报, 2018,42(3):473-479.

11. Chang MX, Zhang J. Alternative Pre-mRNA Splicing in Mammals and Teleost Fish: A Effective Strategy for the Regulation of Immune Responses Against Pathogen Infection. Int J Mol Sci. 2017 Jul 15;18(7). 

12. Hu YW, Wu XM, Ren SS, Cao L, Nie P, Chang MX. NOD1 deficiency impairs CD44a/Lck as well as PI3K/Akt pathway. Sci Rep. 2017 Jun 7;7(1):2979.

13. Wang GL, Wang MC, Zhang XW, Chang MX, Xie HX, Nie P. Molecular cloning, biological effect, and tissue distribution of interleukin-8 protein in mandarin fish (Siniperca chuasti) upon Flavobacterium columnare infection. Fish Shellfish Immunol. 2017 Jul;66:112-119.

14. Wu XM, Hu YW, Xue NN, Ren SS, Chen SN, Nie P, Chang MX. Role of zebrafish NLRC5 in antiviral response and transcriptional regulation of MHC related genes. Dev Comp Immunol. 2017 Mar;68:58-68.

15. Zou PF, Chang MX, Li Y, Xue NN, Li JH, Chen SN, Nie P. NOD2 in zebrafish functions in antibacterial and also antiviral responses via NF-κB, and also MDA5, RIG-I and MAVS. Fish Shellfish Immunol. 2016 Aug;55:173-85.

16. Zhang L, Chen WQ, Hu YW, Wu XM, Nie P, Chang MX. TBK1-like transcript negatively regulates the production of IFN and IFN-stimulated genes through RLRs-MAVS-TBK1 pathway. Fish Shellfish Immunol. 2016 Jul;54:135-43.

17. Xu Q, Jiang Y, Wangkahart E, Zou J, Chang M, Yang D, Secombes CJ, Nie P, Wang T. Sequence and Expression Analysis of Interferon Regulatory Factor 10 (IRF10) in Three Diverse Teleost Fish Reveals Its Role in Antiviral Defense. PLoS One. 2016 Jan 19;11(1):e0147181.

18. Fan Y, Chang MX, Ma J, LaPatra SE, Hu YW, Huang L, Nie P, Zeng L. Transcriptomic analysis of the host response to an iridovirus infection in Chinese giant salamander, Andrias davidianus. Vet Res. 2015 Nov 20;46:136.

19. Zou PF, Chang MX, Li Y, Huan Zhang S, Fu JP, Chen SN, Nie P. Higher antiviral response of RIG-I through enhancing RIG-I/MAVS-mediated signaling by its long insertion variant in zebrafish. Fish Shellfish Immunol. 2015 Mar;43(1):13-24.

20. Chen WQ, Hu YW, Zou PF, Ren SS, Nie P, Chang MX. MAVS splicing variants contribute to the induction of interferon and interferon-stimulated genes mediated by RIG-I-like receptors. Dev Comp Immunol. 2015 Mar;49(1):19-30.

21. Xu QQ, Yang DQ, Tuo R, Wan J, Chang MX, Nie P. Gene cloning and induced expression pattern of IRF4 and IRF10 in the Asian swamp eel (Monopterus albus). Dongwuxue Yanjiu. 2014 Sep;35(5):380-8.

22. Yu ZL, Li JH, Xue NN, Nie P, Chang MX. Expression and functional characterization of PGRP6 splice variants in grass carp Ctenopharyngodon idella. Dev Comp Immunol. 2014 Dec;47(2):264-74.

23. Hu YW, Yu ZL, Xue NN, Nie P, Chang MX. Expression and protective role of two novel NACHT-containing proteins in pathogen infection. Dev Comp Immunol. 2014 Oct;46(2):323-32.

24. Tian JY, Qi ZT, Wu N, Chang MX, Nie P. Complementary DNA sequences of the constant regions of T-cell antigen receptors α, β and γ in mandarin fish, Siniperca chuatsi Basilewsky, and their transcriptional changes after stimulation with Flavobacterium columnare. J Fish Dis. 2014 Feb;37(2):89-101.

25. Chang MX, Zou J, Nie P, Huang B, Yu Z, Collet B, Secombes CJ. Intracellular interferons in fish: a unique means to combat viral infection. PLoS Pathog. 2013;9(11):e1003736.

26. Zou PF, Chang MX, Xue NN, Liu XQ, Li JH, Fu JP, Chen SN, Nie P. Melanoma differentiation-associated gene 5 in zebrafish provoking higher interferon-promoter activity through signalling enhancing of its shorter splicing variant. Immunology. 2014 Feb;141(2):192-202.

27. Li JH, Yu ZL, Xue NN, Zou PF, Hu JY, Nie P, Chang MX. Molecular cloning and functional characterization of peptidoglycan recognition protein 6 in grass carp Ctenopharyngodon idella. Dev Comp Immunol. 2014 Feb;42(2):244-55.

28. Li JH, Chang MX, Xue NN, Nie P. Functional characterization of a short peptidoglycan recognition protein, PGRP5 in grass carp Ctenopharyngodon idella. Fish Shellfish Immunol. 2013 Aug;35(2):221-30.

29. Wang G, Li J, Zou P, Xie H, Huang B, Nie P, Chang M. Expression pattern, promoter activity and bactericidal property of β-defensin from the mandarin fish Siniperca chuatsi. Fish Shellfish Immunol. 2012 Sep;33(3):522-31.

30. Qi ZT, Zhang QH, Wang ZS, Wang AM, Huang B, Chang MX, Nie P. Cloning and expression analysis of a long type peptidoglycan recognition protein (PGRP-L) from Xenopus tropicalis. Dongwuxue Yanjiu. 2011 Aug;32(4):371-8.

31. Zhang SH, Zhu DD, Chang MX, Zhao QP, Jiao R, Huang B, Fu JP, Liu ZX, Nie P. Three goose-type lysozymes in the gastropod Oncomelania hupensis: cDNA sequences and lytic activity of recombinant proteins. Dev Comp Immunol. 2012 Jan;36(1):241-6.

32. Chang M, Collet B, Nie P, Lester K, Campbell S, Secombes CJ, Zou J. Expression and functional characterization of the RIG-I-like receptors MDA5 and LGP2 in Rainbow trout (Oncorhynchus mykiss). J Virol. 2011 Aug;85(16):8403-12.

33. Wei YC, Pan TS, Chang MX, Huang B, Xu Z, Luo TR, Nie P. Cloning and expression of Toll-like receptors 1 and 2 from a teleost fish, the orange-spotted grouper Epinephelus coioides. Vet Immunol Immunopathol. 2011 Jun 15;141(3-4):173-82.

34. Guo HZ, Zou PF, Fu JP, Guo Z, Zhu BK, Nie P, Chang MX. Characterization of two C-type lectin-like domain (CTLD)-containing proteins from the cDNA library of Chinese mitten crab Eriocheir sinensis. Fish Shellfish Immunol. 2011 Feb;30(2):515-24.

35. Chang M, Wang T, Nie P, Zou J, Secombes CJ. Cloning of two rainbow trout nucleotide-binding oligomerization domain containing 2 (NOD2) splice variants and functional characterization of the NOD2 effector domains. Fish Shellfish Immunol. 2011 Jan;30(1):118-27.

36. Xiao FS, Wang YP, Yan W, Chang MX, Yao WJ, Xu QQ, Wang XX, Gao Q, Nie P. Ig heavy chain genes and their locus in grass carp Ctenopharyngodon idella. Fish Shellfish Immunol. 2010 Oct;29(4):594-9.

37. Xu QQ, Chang MX, Sun RH, Xiao FS, Nie P. The first non-mammalian CXCR5 in a teleost fish: molecular cloning and expression analysis in grass carp (Ctenopharyngodon idella). BMC Immunol. 2010 May 26;11:25.

38. Xu QQ, Chang MX, Xiao FS, Huang B, Nie P. The gene and virus-induced expression of IRF-5 in grass carp Ctenopharyngodon idella. Vet Immunol Immunopathol. 2010 Apr 15;134(3-4):269-78.

39. Chen WQ, Xu QQ, Chang MX, Zou J, Secombes CJ, Peng KM, Nie P. Molecular characterization and expression analysis of the IFN-gamma related gene (IFN-gammarel) in grass carp Ctenopharyngodon idella. Vet Immunol Immunopathol. 2010 Apr 15;134(3-4):199-207.

40. Chen WQ, Xu QQ, Chang MX, Nie P, Peng KM. Molecular characterization and expression analysis of nuclear oligomerization domain proteins NOD1 and NOD2 in grass carp Ctenopharyngodon idella. Fish Shellfish Immunol. 2010 Jan;28(1):18-29.

41. Chang MX, Chen WQ, Nie P. Structure and expression pattern of teleost caspase recruitment domain (CARD) containing proteins that are potentially involved in NF-kappaB signalling. Dev Comp Immunol. 2010 Jan;34(1):1-13.

42. Xu Z, Chang MX, Qi ZT, Huang B, Nie P. Immunoglobulin joining (J) chain and its expression in the Chinese soft-shelled turtle (Pelodiscus sinensis). Vet Immunol Immunopathol. 2009 Oct 15;131(3-4):268-72.

43. Zou J, Chang M, Nie P, Secombes CJ. Origin and evolution of the RIG-I like RNA helicase gene family. BMC Evol Biol. 2009 Apr 28;9:85.

44. Chang M, Nie P, Collet B, Secombes CJ, Zou J. Identification of an additional two-cysteine containing type I interferon in rainbow trout Oncorhynchus mykiss provides evidence of a major gene duplication event within this gene family in teleosts. Immunogenetics. 2009 Apr;61(4):315-25.

45. Chang MX, Wang YP, Nie P. Zebrafish peptidoglycan recognition protein SC (zfPGRP-SC) mediates multiple intracellular signaling pathways. Fish Shellfish Immunol. 2009 Feb;26(2):264-74.

46. Liu Y, Chang MX, Wu SG, Nie P. Characterization of C-C chemokine receptor subfamily in teleost fish. Mol Immunol. 2009 Jan;46(3):498-504.

47. Chang MX, Nie P. RNAi suppression of zebrafish peptidoglycan recognition protein 6 (zfPGRP6) mediated differentially expressed genes involved in Toll-like receptor signaling pathway and caused increased susceptibility to Flavobacterium columnare. Vet Immunol Immunopathol. 2008 Aug 15;124(3-4):295-301.

48. Xu ZY, Nie P, Chang MX, Sun BJ. Cloning, characterization and expression analysis of SIMP (source of immunodominant MHC-associated peptides) in grass carp Ctenopharyngodon idella. Fish Shellfish Immunol. 2008 Jun;24(6):701-14.

49. Gao Y, Chang MX, Sun BJ, Nie P. TRAIL in the mandarin fish Siniperca chuatsi: gene and its apoptotic effect in HeLa cells. Fish Shellfish Immunol. 2008 Jan;24(1):55-66.

50. Xu Z, Nie P, Sun B, Chang M. Molecular identification and expression analysis of tumor necrosis factor receptor-associated factor 2 in grass carp Ctenopharyngodon idella. Acta Biochim Biophys Sin (Shanghai). 2007 Nov;39(11):857-68.

51. Xu ZY, Sun BJ, Chang MX, Nie P. Characterization and expression analysis of TNFR-associated factor 1 (TRAF1) in grass carp Ctenopharyngodon idella. Vet Immunol Immunopathol. 2008 Jan 15;121(1-2):44-57.

52. Li L, Chang MX, Nie P. Molecular cloning, promoter analysis and induced expression of the complement component C9 gene in the grass carp Ctenopharyngodon idella. Vet Immunol Immunopathol. 2007 Aug 15;118(3-4):270-82.

53. Chang MX, Nie P, Wei LL. Short and long peptidoglycan recognition proteins (PGRPs) in zebrafish, with findings of multiple PGRP homologs in teleost fish. Mol Immunol. 2007 Apr;44(11):3005-23.

54. Sun BJ, Chang MX, Song Y, Yao WJ, Nie P. Gene structure and transcription of IRF-1 and IRF-7 in the mandarin fish Siniperca chuatsi. Vet Immunol Immunopathol. 2007 Mar 15;116(1-2):26-36.

55. Chang MX, Nie P. Intelectin gene from the grass carp Ctenopharyngodon idella: cDNA cloning, tissue expression, and immunohistochemical localization. Fish Shellfish Immunol. 2007 Jul;23(1):128-40.

56. Chang MX, Sun BJ, Nie P. The first non-mammalian CXCR3 in a teleost fish: gene and expression in blood cells and central nervous system in the grass carp (Ctenopharyngodon idella). Mol Immunol. 2007 Feb;44(6):1123-34.

57. Sun B, Chang M, Chen D, Nie P. Gene structure and transcription of IRF-2 in the mandarin fish Siniperca chuatsi with the finding of alternative transcripts and microsatellite in the coding region. Immunogenetics. 2006 Sep;58(9):774-84.

58. Chang MX, Nie P, Xie HX, Wang GL, Gao Y. Characterization and expression analysis of TNF-related apoptosis inducing ligand (TRAIL) in grass carp Ctenopharyngodon idella. Vet Immunol Immunopathol. 2006 Mar 15;110(1-2):51-63.

59. Xie HX, Nie P, Chang MX, Liu Y, Yao WJ. Gene cloning and functional analysis of glycosaminoglycan-degrading enzyme chondroitin AC lyase from Flavobacterium columnare G4. Arch Microbiol. 2005 Oct;184(1):49-55.

60. Chang MX, Nie P, Liu GY, Song Y, Gao Q. Identification of immune genes in grass carp Ctenopharyngodon idella in response to infection of the parasitic copepod Sinergasilus major. Parasitol Res. 2005 Jun;96(4):224-9.

61. Chang MX, Nie P, Sun BJ, Yao WJ. Molecular cloning of TRAF2 binding protein gene and its promoter region from the grass carp Ctenopharyngodon idellus. Vet Immunol Immunopathol. 2005 May 1;105(1-2):105-13.

62. Chang MX, Nie P, Xie HX, Sun BJ, Gao Q. Characterization of two genes encoding leucine-rich repeat-containing proteins in grass carp Ctenopharyngodon idellus. Immunogenetics. 2005 Jan;56(10):710-21.

63. Luo HL, Nie P, Chang MX, Song Y, Yao WJ. Characterization of development-related genes for the cestode Bothriocephalus acheilognathi. Parasitol Res. 2004 Oct;94(4):265-274.

1. pathogen recognition receptors for innate immune recognition and signal transduction

2. The role of NLR inflammasome in host defense

3. Functional comparison of innate immune signaling pathways in vertebrate

The Forum of Molecular Immunology of Aquatic Animals; Shanghai, 15th October 2018

2017 Sino-Micro Annual Symposium;Xi'an, China, June 29-July 1, 2017

1ST INTERNATIONAL CONFERENCE OF FISH AND SHELLFISH IMMUNOLOGY (ISFSI) ；Vigo  (Galicia,Spain), 25-28th June 2013

已指导学生

薛娜娜  硕士研究生  430139-生物工程  

余章龙  硕士研究生  071004-水生生物学  

任诗思  硕士研究生  071004-水生生物学  

胡祎玮  博士研究生  071004-水生生物学  

现指导学生

武小曼  博士研究生  090801-水产养殖  

方红  硕士研究生  071004-水生生物学  

李鹏伟  博士研究生  090801-水产养殖  

曹璐  博士研究生  071004-水生生物学