General

Name:    Jin-Qiu Zhou (周金秋)

Address:  Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, 320 Yueyang Road, Shanghai, China

E-mail:    zhoulab@sibcb.ac.cn

部门/实验室:生物化学与细胞生物学研究所

Research Areas

histone modification; chromatin remodeling; telomere; cellular aging; cellular memory

Education

08/92-05/97   Ph.D. in Biochemistry and Molecular Biology, University of Miami School of Medicine, USA     

09/84-07/88   B.S. in Zoology Nanjing University, Nanjing, China

Experience

   
Work Experience

09/01-present   Principal Investigator, Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences.

02/02-01/07     Leader of Max Planck Junior Scientist Group at the Institute of Biochemistry and Cell Biology.

06/98-09/01     Postdoctoral Fellow, Princeton University Department Molecular Biology (with Dr. Virginia A. Zakian, Telomere structure, function and replication).

06/97-05/98     Postdoctoral Fellow, University of Miami School of Medicine, Department of Medicine (with Drs. Antero G. So and Kathleen M. Downey, Eukaryotic DNA replication and repair).

08/88-07/92     Research Assistant, Shanghai Institute of Cell Biology, Chinese Academy of Sciences, China (with Profs. Hsiujeng Yeh and Yong-Hua Xu, Function of PDGF in tumor formation).

Teaching Experience

2002-2012     Teaching the topic of “Chromosome, chromatin and telomeres” in the course of Bio2000-Molecular Biology at SIBS and Tsinghua/Beijing University (every Fall semester) 

2005 (Spring)   Teaching the topic of “Molecular Mechanisms of Cell Cycle Regulation” in the course of Molecular Genetics at The Graduate School of Chinese Academy of Sciences, Beijing

Publications

1.    Zhou Z, Liu YT, Ma L, Gong T, Hu YN, Li HT, Cai C, Zhang LL, Wei G, Zhou JQ#. (2017) Independent manipulation of histone H3 modifications in individual nucleosomes reveals the contributions of sister histones to transcription. eLife 6:e30178 DOI: 10.7554/eLife.30178

2.    Liu XG, Wei W, Liu YT, Yang XL, Wu J, Zhang Y, Zhang Q, Shi TL, James Du X, Zhao YM, Lei M, Zhou JQ, Li JW#, Wong JM#. (2017) MOF as an evolutionarily conserved histone crotonyltransferase and transcriptional activation by histone acetyltransferase-deficient and crotonyltransferase-competent CBP/p300. Cell Discovery 3, 17016

3.    Wu ZF, Liu J, Zhang QD, Lv DK, Wu NF, Zhou JQ#. (2017) Rad6–Bre1-mediated H2B ubiquitination regulates telomere replication by promoting telomere-end resection. Nucleic Acids Res. 45(6):3308-3322

4.    Liu J, He MH, J Peng J, Duan YM, Lu YS, Wu ZF, Gong T, Li HT, Zhou JQ#. (2016) Tethering Telomerase to Telomeres Increases Genome Instability and Promotes Chronological Aging in Yeast. Aging 8(11): 2827-2847

5.    Duan YM, Zhou B-O, Peng J, Tong XJ, Zhang QD, Zhou JQ#. (2016) Molecular dynamics of de novo telomere heterochromatin formation in budding yeast. Journal of Genetics and Genomics 43(7): 451-465

6.    Li HT, Gong T, Zhou Z, Liu YT, Cao XW, He YN, Charlie Chen DG#, Zhou JQ#. (2015) Yeast Hmt1 catalyses asymmetric dimethylation of histone H3 arginine 2 in vitro. Biochem. J. 467(3):507-515

7.   Peng J, He MH, Duan YM, Liu YT, Zhou JQ#. (2015) Inhibition of Telomere Recombination by Inactivation of KEOPS Subunit Cgi121 Promotes Cell Longevity. PLOS Genet. 11(3):e1005071

8.    Fu XH, Duan YM, Liu YT, Cai C, Meng FL, Zhou JQ#. (2014) Telomere recombination preferentially occurs at short telomeres in telomerase-null type II survivors. PloS One 9(3):e90644

9.    Hu Y, Tang HB, Liu NN, Tong XJ, Dang W, Duan YM, Fu XH, Zhang Y, Peng J, Meng FL, Zhou JQ#. (2013) Telomerase-null survivor screening identifies novel telomere recombination regulators. PLOS Genet. 9(1):e1003208

10.  Li QJ, Tong XJ, Duan YM, Zhou JQ#. (2013) Characterization of the intramolecular G-quadruplex promoting activity of Est1. FEBS Letter 587(6):659-665

11.  Peng J and Zhou JQ#. (2012) The tail-module of yeast Mediator complex is required for telomere heterochromatin maintenance. Nucleic Acids Res. 40: 581-593

12. Yang H, Zhang TL, Tao Y, Wu LJ, Li HT, Zhou JQ, Zhong C and Ding JP. (2012) Saccharomyces cerevesiae MHF complex structurally resembles the histones (H3-H4)2 heterotetramer and functions as a heterotetramer. Structure. 20: 364-370

13.  Zhou BO and Zhou JQ#. (2011) Recent transcription-induced histone H3K4 methylation inhibits gene reactivation. J. Biol. Chem. 286: 34770-34776

14.   Wang SS, Zhou BO and Zhou JQ#. (2011) Histone H3 Lysine 4 hypermethylation prevents aberrant nucleosome remodeling at the PHO5 promoter. Mol. Cell. Biol. 31: 3171-3181

15.   Tong XJ, Li QJ, Duan YM, Liu NN, Zhang ML and Zhou JQ#. (2011) Est1 protects telomeres and inhibits subtelomeric Y’-element recombination. Mol. Cell. Biol. 31(6): 1263-1274

16.   Zhou BO, Wang SS, Zhang Y, Fu XH, Dang W, Lenzmeier BA and Zhou JQ#. (2011) Histone H4 Lysine 12 Acetylation Regulates Telomeric Heterochromatin Plasticity in Saccharomyces cerevisiae. PLOS Genet. 7: e1001272

17.   Bian CB, Xu C, Ruan JB, Lee KK, Burke TL, Tempel W, Barsyte D, Li J, Wu MH, Zhou BO, Fleharty BE, Paulson A, Allali-Hassani A, Zhou JQ, Mer G, Grant PA, Workman JL, Zang JY and Min JR. (2011) Sgf29 binds histone H3K4me2/3 and is required for SAGA complex recruitment and histone H3 acetylation. The EMBO J. 30: 2829 – 2842

18.   Chang YY, Wu J, Tong XJ, Zhou JQ and Ding JP. (2011) Crystal structure of the catalytic core of Saccharomyces cerevesiae histone demethylase Rph1: insights into the substrate specificity and catalytic mechanism”, Biochem. J. 433: 295-302

19.   Zhou BO, Wang SS, Xu LX, Meng FL, Xuan YJ, Duan YM, Wang JY, Hu H, Dong XC, Ding JP and Zhou JQ#. (2010) SWR1 complex poises heterochromatin boundaries for antisilencing activity propagation. Mol. Cell. Biol. 30: 2391-2400

20. Liu NN, Han TX, Du LL and Zhou JQ#. (2010) A genome-wide screen for Schizosaccharomyces pombe deletion mutants that affect telomere length. Cell Res. 20:963-965

21.   Meng FL, Chen XF, Hu Y, Tang HB, Dang W and Zhou JQ#. (2010) Sua5p is required for telomere recombination in Saccharomyces cerevisiae. Cell Res. 20:495-498

22.   Zhang ML, Tong XJ, Fu XH, Zhou B, Wang JY, Liao XH, Li QJ, Shen N, Ding JP and Zhou JQ#. (2010) Yeast telomerase subunit Est1p has guanine quadruplex–promoting activity that is required for telomere elongation. Nat. Struct. Mol. Biol. 17: 202-209

23.   Liu YW, Huang HD, Zhou BO, Wang SS, Hu YX, Li Y, Liu JP, Zang JY, Niu LW, Wu JH, Zhou JQ, Teng MK, and Shi YY. (2010) Structural Analysis of Rtt106p Reveals a DNA Binding Role Required for Heterochromatin Silencing. J. Biol. Chem. 285: 4251-4262

24.   Hu JL, Zhou BO, Zhang RR, Zhang KL, Zhou JQ# and Xu GL#. (2009) The N-terminus of histone H3 is required for de novo DNA methylation in chromatin. Proc. Natl. Acad. Sci. 106: 187-192

25.   Meng FL, Hu Y, Shen N, Tong XJ, Wang JY, Ding JP and Zhou JQ#. (2009) Sua5p a single-stranded telomeric DNA-binding protein facilitates telomere replication. EMBO J. 28: 1466-1478

26.   Qian W, Wang JY, Jin NN, Fu XH, Lin YC, Lin JJ and Zhou JQ#. (2009) Ten1p promotes the telomeric DNA binding acivity of Cdc13p: an implication for its function in telomere length regulation. Cell Res. 19: 850-863

27.   Zhou J, Zhou BO, Lenzmeier BA and Zhou JQ#. (2009) Histone deacetylase Rpd3 antagonizes Sir2-dependent silent chromatin propagation. Nucleic Acids Res. 37: 3699-3713

28.   Chen XF, Meng FL and Zhou JQ#. (2009) Telomere recombination accelerates cellular aging in Saccharomyces cerevisiae. PLOS Genet. 5: e1000535

29.   Fu XH, Meng FL, Hu Y and Zhou JQ#. (2008) Candida albicans, a distinctive fungal model for cellular aging study. Aging Cell 7:746-757

30.   Yang CP, Chen YB, Meng FL and Zhou JQ#. (2006) Saccharomyces cerevisiae Est3p dimerizes in vitro and dimerization contributes to efficient telomere replication in vivo. Nucleic Acids Res. 34:407-416

31.   Zhang DH, Zhou B, Huang Y, Xu LX and Zhou JQ#. (2006) The human Pif1 helicase, a potential Escherichia coli RecD homologue, inhibits telomerase activity. Nucleic Acids Res. 34:1393-1404

32.   Zhang P, Du JM, Sun BF, Dong XC, Xu GL, Zhou JQ, Huang QH, Liu Q, Hao Q, and Ding JP. (2006) Crystal structure of human MRG15 chromo domain reveals its binding with Lys36-methylated histone H3, Nucleic Acids Res. 34: 6621-6628

33.   Wang YL, Qiu W, Feng HC, Li YX, Zhuang LZ, Wang Z, Liu Y, Zhou JQ, Zhang DH, Tsao GS. (2006) Immortalization of normal human cytotrophoblast cells by reconstitution of telomeric reverse transcriptase activity. Mol Hum Reprod. 12(7):451-460

34.   Chen YB, Yang CP, Li RX, Zeng R and Zhou JQ#. (2005) Def1p is involved in telomere maintenance in budding yeast. J. Biol. Chem. 280:24784-24791

35.   Liao XH, Zhang ML, Yang CP, Xu LX and Zhou JQ#. (2005) Characterization of recombinant Saccharomyces cerevisiae telomerase core enzyme purified from yeast. Biochem. J. 390:169-176

36.   Lu X, Tan CK, Zhou JQ, You M, Carastro LM, Downey KM and So AG. (2002) Direct interaction of proliferating cell nuclear antigen with the small subunit of DNA polymerase δ. J. Biol. Chem. 277, 24340-24345

37.   Zhou JQ*, Qi H*, Schulz VP, Mateyak MK, Monson EK and Zakian VA. (2002) Schizosaccharomyces pombe pfh1(+) Encodes an Essential 5’ to 3’ DNA Helicase That Is a Member of the PIF1 Subfamily of DNA Helicases. Mol. Biol. Cell. 13:2180-2191 (*Co-first author)

38.   Ivessa AS*, Zhou JQ*, Schulz VP, Monson EK and Zakian VA. (2002) Saccharomyces Rrm3p, a 5’ to 3’ DNA helicase that promotes replication fork progression through telomeric and subtelomeric DNA. Genes & Dev. 16:1383-1396 (*Co-first author)

39.   Zhou JQ, Monson EK, Teng SC, Schulz VP and Zakian VA. (2000) Pif1p Helicase, a Catalytic Inhibitor of Telomerase in Yeast. Science 289: 771-774

40.   Ivessa AS*, Zhou JQ* and Zakian VA. (2000) The Saccharomyces Pif1p DNA helicase and the highly related Rrm3p have opposite effects on replication fork progression in ribosomal DNA. Cell 100: 479-489 (*Co-first author)

41.   Zhou JQ, He H, Tan CK, Downey KM and So AG. (1997) The small subunit is required for functional interaction of DNA polymerase delta with the proliferating cell nuclear antigen. Nucleic Acids Res. 27:1094-1099

42.   Zhou JQ, Tan CK, So AG and Downey KM. (1996) Purification and characterization of the human catalytic subunit of human DNA polymerase delta expressed in baculovirus-infected insect cells. J. Biol. Chem. 271:29740-29745

Papers


Patents
   

Research Interests

The long-term goal of the lab is to understand the processes that eukaryotic cells faithfully maintain their chromosomes to avoid chromosomal instability. Currently, most of our work has been focused on the structure, function and replication of telomeres mainly using the baker’s yeast as a model. We use a combination of genetic, biochemical and cell biological approaches to address (1) how telomere length and structure are maintained, and the biological relevance of telomere regulation to genome stability and cellular ageing; (2) how a cell establishes the boundary between the telomere silent chromatin and adjacent active chromatin to prevent the telomeric hetrochromatin spreading; (3) what epigenetic codes and information are employed when telomere replication or DNA double-strand break occurs; (4) what is the molecular basis for telomere-length-dependent or -independent cellular aging.

Conferences

11/2003   Invited speaker in the 6th Conference of Asia-Pacific International Molecular Biology Network (A-IMBN), Tokyo, Japan (Title of the talk: Pif1 sub-family helicases and telomere replication)

04/2004   Invited speaker in EMBO Workshop/58th Harden Conference - Telomeres and Genome Stability, Oxford, UK (Title of the talk: Rrm3p interaction protein-Rip1p has function in both telomere and rDNA)

11/2004   Invited speaker in the 17th FAOBMB Symposium/2nd IUBMB Special Meeting/7th A-IMBM Conference on “Genomics and Health in the 21st Century”, Bangkok, Thailand (Title of the talk: Def1p Cooperates with yKu To Protect Telomeres)

05/2005   Invited speaker in “Telomeres and Telomerase”, Cold Spring Harbor, USA (Title of the talk: human Pif1, a DNA helicase, inhibits telomerase to regulate telomere length)

07/2006   Invited speaker in Symposium on the Frontier Biomedical Science, Taiwan (Title of the talk: The functional interaction of Est3p with Est1p is essential for telomerase activity in Saccharomyces cerevisiae)

10/2006   Invited speaker in the 5th Asian-Pacific Organization of Cell Biology Congress, Beijing, China (Title of the talk: The Est3p and Est1p interaction is important for telomerase activity in Saccharomyces cerevisiae)

02/2007   Invited speaker in the 1st Australia-China Biomedical Research Conference, Melbourne, Australia (Title of the talk: The Arg110 of Est3p is a critical residue for its function in telomerase activity in Saccharomyces cerevisiae)

10/2007   Invited Speaker, the 66th Annual Meeting of the Japanese Cancer Association, Oct 3-5, Yakohama, Japan (Title of the talk: The function of Est1p and Est3p in regulation of telomerase activity in Saccharomyces cerevisiae).

07/2008   Invited speaker in the 15th East Asia Joint Conference on Biomedical Research, Seoul, Korea (Title of the talk: The function of Est1p in regulation of telomerase activity in Saccharomyces cerevisiae)

11/2008   Invited Seminar in National Institute for Biological Sciences, Beijing, China (Title of the talk: Telomerase activity regulation in Saccharomyces cerevisiae)

07/2009   Invited speaker in “Science 100” Forum, Lanzhou, China (Title of the talk: Sua5p a Conserved Single-stranded Telomeric DNA Binding Protein Facilitates Telomere Replication)

05/2010   Invited speaker in Mini-Symposium on Chromatin and Epigenetics, Shanghai, China (Title of the talk: Histone H4 lysine 12 acetylation regulates telomere plasticity in Saccharomyces cerevisiae)

08/2010   Invited speaker in Symposium on Gene and Genome, Shanghai, China (Title of the talk: Histone H4 lysine 12 acetylation regulates telomere dynamics in Saccharomyces cerevisiae)

12/2010   Invited speaker in the 4th Cao Tian-Qin Memorial Symposium on Protein Research, Xiamen, China (Title of the talk: Yeast telomerase subunit Est1 plays dual roles in telomerase activation and telomere protection)

07/2011   Invited speaker in Shanghai Symposium: Signaling, Inflammation and Cancer, Shanghai, China (Title of the talk: The effect of G-quadruplex structure on telomere replication and recombination)

10/2011   Invited speaker in 2nd Sino-German Symosium on “DNA Repair and Human Disease”, Mainz, Germany (Title of the talk: Telomerase-null survivor screening identifies novel telomere recombination pathways)

04/2012   Invited speaker in 4Th International Conference on Biomedical and Environmental Sciences & Technology, Guangzhou, China (Title of the talk: Telomerase-null survivor screening identifies novel telomere recombination pathways)

04/2012   Invited speaker in Yeast Meeting-2012, Beijing, China (Title of the talk: Yeast telomere heterochromatin structure and function)

08/2012          Invited speaker in International Symosium of Asian Association of Aging Research 2012 in Fukuoka-Biology of Aging and Disorders, Fukuoka, Japan (Title of the talk: Telomere recombination in regulation of replicative life span in Saccharomyces cerevisiae)

09/2013          Invited speaker in International Meeting on Message from yeast to Epigenetics~Yeast clarifies the frontiers of life science~, Fukui, Japan (Title of the talk: Molecular Dynamics of Telomeric Heterochromatin Formation)

09/2013   Invited speaker in Cold Spring Harbor Asia conference on Molecular Basis of Aging and Disease, Suzhou, China (Title of the talk: The Inhibition of Telomere Recombination Delays Cellular Aging in Yeast)

05/2014   Invited speaker in Cold Spring Harbor Asia conference on Epigenetics, Chromation and Transcription, Suzhou, China (Title of the talk: HMT1 Dimer  Fulfils Methylation of Histone H3 Arginine 2 in Trans)

06/2015   Invited speaker in Society of Chinese Bioscientists in America, The 15th International Symposium, Taipei, China (Title of the talk: Inhibition of Telomere Recombination Promotes Cell Longevity)

05/2016   Invited speaker in Cold Spring Harbor Asia conference on Epigenetics, Chromation and Transcription, Suzhou, China (Title of the talk: Asymmertrically Modified Nucleosomes Poise Gene Activation/ Repression in Yeast)

08/2016   Invited speaker in Symosium on 7th China Microbial Genetics, Nei Mongol, China (Title of the talk: Asymmetrically modified nucleosomes poise genes for activation/repression in yeast)

09/2016   Invited speaker in Cold Spring Harbor Asia conference on Telomere and Telomerase, Suzhou, China (Title of the talk: Rad–Bre1 Mediated H2B Ubiquitination Regulates Telomere Replication by Promoting Telomere- end resection)

06/2017   Invited speaker in 12th China Gene function and epigenetic regulation, Beijing, China (Title of the talk: Modifications on both sister histone H3s coordinate to regulate chromatin structure and transcription)

08/2017   Invited speaker in 10th China model fungi Conference, Chengdu, China (Title of the talk: KEOPS Complex Affects DNA Double-Strand Break Repair by Promotion DNA End Resection)

10/2017   Invited speaker in 3rd Frontier of Genetics and Genomics, ShanghaiTech Univerisity, Shanghai, China (Title of the talk: Independent manipulation of histone H3 modifications in individual nucleosomes reveals the contributions of sister histones to transcription)


Collaboration

   

Students

已指导学生

张登宏  01  19183  

陈勇彬  01  19182  

付晓虹  01  19183  

廖新化  01  19183  

杨翠萍  01  19183  

张明亮  01  19183  

钱慰  01  19183  

黄妤  02  19182  

孟飞龙  01  19183  

陈小芬  01  19183  

周波  01  19183  

童夏静  01  19183  

胡琰  01  19183  

王珊珊  01  19183  

刘宁宁  01  19183  

彭晶  01  19183  

段一民  01  19183  

周振  01  19183  

现指导学生

刘雨婷  01  19183  

何明洪  01  19183  

张伶俐  01  19183  

刘颖颖  01  19183  

蔡琛  01  19183  

吴年凤  02  19183  

李亭宜  02  19183  

张琼娣  01  19183  

吴至竞   02  19182  

刘家程  01  19183  

温洋  01  19183  

Honors & Distinctions

1992-1997  University of Miami Graduate Fellowship

2001-2004  One Hundred Talent Project Award from Chinese  Academy of Sciences

2001-2005  Distinguished Young Scientist Award from National Natural Science Foundation ofChina

2011            Zhu Li yuehua Excellent Teacher Award

2011        Arthur Kornberg Memorial Lecture Award from A-IMBN (Asia-Pacific International Molecular Biology Network)