主要研究领域为1. 猪的基因组编辑； 2. 猪疾病模型建立和经济性状功能基因研究；3. 猪早期胚胎发育中的分子事件。通过建立高效的猪基因组编辑和功能基因组学分析平台，可以为生物医学研究和临床前转化提供理想的疾病模型，同时也可以为猪的经济性状改良和品系培育提供新的手段和解决思路。目前已经建立了包括人的色素性视网膜炎的疾病模型、听力缺陷小型猪疾病模型以及多个出生缺陷和帕金森猪疾病模型；创建了携带有人抗凝血因子9, Furin蛋白和α-抗胰蛋白酶的转基因猪。首次报道利用组蛋白去乙酰化抑制剂-Scriptaid大幅提高了在生物医学研究中具有广泛应用价值的-NIH近交系小型猪的克隆效率。首次报道了利用锌指核酶技术在大动物当中实现了GFP基因的高效敲除并且利用CRISPR/Cas9实现了猪基因组单一基因或者多个基因的同时编辑。

   

071008-发育生物学

转基因动物
体细胞重编程
早期胚胎发育

   

2010-08--今 中国科学院动物研究所 研究员
2007-07--2010-08 University of Missouri-Columbia Research Assistant Professor
2005-11--2007-06 University of New Orleans Postdoc
2003-08--2005-11 上海交通大学医学遗传研究所 助理研究员

生殖生物学

   

[1] 赵建国, 张颖, 戚亚男, 田双杰, 宗若君, 闫星慧, 王煜. 一种引导编辑系统和目的基因序列的定点修饰方法. CN: CN116716298A, 2023-09-08.
[2] 赵建国, 王霄, 宋瑞高, 刘强, 秦国嵩, 姚婧, 张颖. 一种猪TMC1突变基因及其用途. CN: CN115181170A, 2022-10-14.
[3] , , , , . Cold-resistant and lean-type transgenic pig and preparation method therefor. CN: US11419320B2, 2022-08-23.
[4] 赵建国, 孙泽文, 张颖, 王霄, 秦国嵩, 姚婧. 突变基因及其用途. CN: CN114181944A, 2022-03-15.
[5] 赵建国, 王霄, 曹春伟, 海棠, 周琪, 王红梅, 张颖, 贾启涛, 郑千涛. 一种突变基因及其用于构建斑色鱼鳞癣小型猪模型的用途. CN: CN110846321B, 2021-11-19.
[6] 周琪, 赵建国, 海棠, 贾启涛, 曹春伟, 王红梅, 张颖, 郑千涛, 王霄. 一种突变基因及其用于构建蹼足病小型猪模型的用途. CN: CN110846330B, 2021-08-27.
[7] 郑爱华, 赵超越, 李虹悦, 张毓航, 金万洙, 赵建国. 基于水疱性口炎病毒载体的重组新型冠状病毒及其制备方法与应用. CN: CN112941038A, 2021-06-11.
[8] 赵建国. 突变基因及构建短肢性侏儒症小型猪模型的方法和用途. ZL202010959862.2, 2020-09-14.
[9] 赵建国, 郑千涛, 黄娇娇, 秦国嵩, 金万洙. 一种抗寒及瘦肉型转基因猪及其制备方法. CN: CN107182940A, 2017-09-22.
[10] 王红梅, 周琪, 赵建国, 孟安明, 曹春伟, 海棠, 祁蒙. 一种猪MITF基因突变的检测方法. CN: CN107034268B, 2017-08-11.
[11] 赵建国, 姚婧, 黄娇娇, 秦国嵩. 一种敲除猪DJ-1基因的细胞的构建方法及其应用. CN: CN107034192A, 2017-08-11.
[12] 何宏轩, 王玉田, 王承民, 贾亚雄, 马志军, 赵建国. 一种消毒剂及其制备方法和用途. CN: CN105519588A, 2016-04-27.

   

[1] Zhao, Ying, Chen, Chuanhe, Pan, Jianfei, Lam, Sin Man, Shui, Guanghou, Yang, Shulin, Wu, Tianwen, Yang, Ning, Tao, Cong, Zhao, Jianguo, Wang, Yanfang. Adipocyte Rnf20 ablation increases the fast-twitch fibers of skeletal muscle via lysophosphatidylcholine 16:0. CELLULAR AND MOLECULAR LIFE SCIENCES[J]. 2023, 80(9): http://dx.doi.org/10.1007/s00018-023-04896-4.
[2] Qi, Yanan, Zhang, Ying, Tian, Shuangjie, Zong, Ruojun, Yan, Xinghui, Wang, Yu, Wang, Yanfang, Zhao, Jianguo. An optimized prime editing system for efficient modification of the pig genome. SCIENCE CHINA-LIFE SCIENCES. 2023, http://dx.doi.org/10.1007/s11427-022-2334-y.
[3] Chuanmin Qiao, Maozhang He, Shumei Wang, Xinjie Jiang, Feng Wang, Xinjian Li, Shuyi Tan, Zhe Chao, Wenshui Xin, Shuai Gao, Jingli Yuan, Qiang Li, Zichun Xu, Xinli Zheng, Jianguo Zhao, Guangliang Liu. Multi-omics analysis reveals substantial linkages between the oral-gut microbiomes and inflamm-aging molecules in elderly pigs. FRONTIERS IN MICROBIOLOGY[J]. 2023, 14: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10542583/.
[4] 高菲, 王煜, 杜嘉祥, 杜旭光, 赵建国. 遗传修饰猪模型在生物医学及农业领域研究进展及应用. 遗传[J]. 2023, 45(01期): 6-28, VIP_JournalArticle.
[5] 赵建国. One-step base editing in multiple genes by direct embryo injection for pig trait improvement. SCIENCE CHINA Life Sciences[J]. 2022, [6] Liu, Wenwen, Chen, Min, Liu, Chao, Wang, Liying, Wei, Huafang, Zhang, Ruidan, Ren, Zhengxing, Chen, Yinghong, Luo, Mengcheng, Zhao, Jianguo, Jiang, Hongwei, Gao, Fei, Li, Wei. Epg5 deficiency leads to primary ovarian insufficiency due to WT1 accumulation in mouse granulosa cells. AUTOPHAGY. 2022, 19(2): 644-659, [7] Yao, Jing, Wang, Yu, Cao, Chunwei, Song, Ruigao, Bi, Dengfeng, Zhang, Hongyong, Li, Yongshun, Qin, Guosong, Hou, Naipeng, Zhang, Nan, Zhang, Jin, Guo, Weiwei, Yang, Shiming, Wang, Yanfang, Zhao, Jianguo. CRISPR/Cas9-mediated correction of MITF homozygous point mutation in a Waardenburg syndrome 2A pig model. MOLECULAR THERAPY-NUCLEIC ACIDS[J]. 2021, 24: 986-999, http://dx.doi.org/10.1016/j.omtn.2021.04.009.
[8] 宋瑞高, 刘克强, 王煜, 秦国嵩, 肖萌, 刘雅萍, 杨俊, 宗若君, 闫星彗, 郑千涛, 张洪勇, 侯乃鹏, 姚婧, 王霄, 王彦芳, 赵建国, 张学. 家族性反常性痤疮猪模型的创制及其致病机理的研究. 科学通报:英文版[J]. 2021, 66(23): 2343-2346, http://lib.cqvip.com/Qikan/Article/Detail?id=7106029502.
[9] Bi, Dengfeng, Yao, Jing, Wang, Yu, Qin, Guosong, Zhang, Yunting, Wang, Yanfang, Zhao, Jianguo. CRISPR/Cas13d-mediated efficient KDM5B mRNA knockdown in porcine somatic cells and parthenogenetic embryos. REPRODUCTION[J]. 2021, 162(2): 149-160, [10] Song, Ruigao, Liu, Keqiang, Wang, Yu, Qin, Guosong, Xiao, Meng, Liu, Yaping, Yang, Jun, Zong, Ruojun, Yan, Xinghui, Zheng, Qiantao, Zhang, Hongyong, Hou, Naipeng, Yao, Jing, Wang, Xiao, Wang, Yanfang, Zhao, Jianguo, Zhang, Xue. A pig model carrying heterozygous point mutation of NCSTN simulates familial acne inversa and reveals dysregulated cholesterol biosynthesis via the Notch-pAMPK-HMGCR pathway. SCIENCE BULLETIN[J]. 2021, 66(23): 2343-2346, http://dx.doi.org/10.1016/j.scib.2021.05.022.
[11] 赵建国. Mitochondrial Uncoupling Protein-1 Antagonizes Atherosclerosis by Blocking NLRP3-Inflammasome-dependent Interleukin-1β production. Science Advances. 2021, [12] 毕登峰, 王煜, 姚婧, 赵建国. CRISPR/Cas13d介导猪胎儿成纤维细胞SUV39H1/SUV39H2基因敲降. 中国畜牧兽医[J]. 2021, 48(6): 1957-1966, http://lib.cqvip.com/Qikan/Article/Detail?id=7105024540.
[13] Wang, Yu, Bi, Dengfeng, Qin, Guosong, Song, Ruigao, Yao, Jing, Cao, Chunwei, Zheng, Qiantao, Hou, Naipeng, Wang, Yanfang, Zhao, Jianguo. Cytosine Base Editor (hA3A-BE3-NG)-Mediated Multiple Gene Editing for Pyramid Breeding in Pigs. FRONTIERS IN GENETICS[J]. 2020, 11: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7701296/.
[14] Li, Feifei, Wang, Danyang, Song, Ruigao, Cao, Chunwei, Zhang, Zhihua, Wang, Yu, Li, Xiaoli, Huang, Jiaojiao, Liu, Qiang, Hou, Naipeng, Xu, Bingxiang, Li, Xiao, Gao, Xiaomeng, Jia, Yan, Zhao, Jianguo, Wang, Yanfang. The asynchronous establishment of chromatin 3D architecture between in vitro fertilized and uniparental preimplantation pig embryos. GENOME BIOLOGY[J]. 2020, 21(1): http://dx.doi.org/10.1186/s13059-020-02095-z.
[15] 赵建国. Rnf20 deficiency in adipocyte impairs adipose tissue development and thermogenesis. protein cell. 2020, [16] 王煜, 宋瑞高, 赵建国, 王彦芳. 碱基编辑器介导的猪IGF2基因高效定点突变. 中国畜牧兽医[J]. 2020, 47(11): 3427-3435, http://lib.cqvip.com/Qikan/Article/Detail?id=7103246372.
[17] Ruigao SONG, Yu WANG, Yanfang WANG, Jianguo ZHAO. Base editing in pigs for precision breeding. 农业科学与工程前沿:英文版[J]. 2020, 7(2): 161-170, http://lib.cqvip.com/Qikan/Article/Detail?id=7102483705.
[18] Liu, Lulu, Wang, Yu, Liang, Xiaojuan, Wu, Xiao, Liu, Jiali, Yang, Shulin, Tao, Cong, Zhang, Jin, Tian, Jianhui, Zhao, Jianguo, Wang, Yanfang. Stearoyl-CoA Desaturase Is Essential for Porcine Adipocyte Differentiation. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES[J]. 2020, 21(7): https://doaj.org/article/19a125e138e1471898c1e52bbcc524a0.
[19] 潘建飞, 郑千涛, 陶聪, 赵建国, 王彦芳. 急性冷刺激后巴马猪背膘和腹股沟皮下脂肪组织的脂质组比较. 中国畜牧兽医[J]. 2020, 47(9): 2759-2766, http://lib.cqvip.com/Qikan/Article/Detail?id=7102893426.
[20] Zhao, JianGuo, Wang, DongHui. beta-Selective Cu(II)-Catalyzed Dehydrogenative Enamination of Alkylbenzenes. ORGANIC LETTERS[J]. 2020, 22(24): 9473-9477, https://www.webofscience.com/wos/woscc/full-record/WOS:000607016900014.
[21] Jianfei Pan, Cong Tao, Chunwei Cao, Qiantao Zheng, Sin Man Lam, Guanghou Shui, Xuexue Liu, Kui Li, Jianguo Zhao, Yanfang Wang. Adipose lipidomics and RNA-Seq analysis revealed the enhanced mitochondrial function in UCP1 knock-in pigs. BBA - MOLECULAR AND CELL BIOLOGY OF LIPIDS. 2019, 1864(10): 1375-1383, http://dx.doi.org/10.1016/j.bbalip.2019.06.017.
[22] Pan, Jianfei, Tao, Cong, Cao, Chunwei, Zheng, Qiantao, Lam, Sin Man, Shui, Guanghou, Liu, Xuexue, Li, Kui, Zhao, Jianguo, Wang, Yanfang. Adipose lipidomics and RNA-Seq analysis revealed the enhanced mitochondrial function in UCP1 knock-in pigs. BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR AND CELL BIOLOGY OF LIPIDS[J]. 2019, 1864(10): 1375-1383, http://dx.doi.org/10.1016/j.bbalip.2019.06.017.
[23] Jianguo Zhao, Liangxue Lai, Weizhi Ji, Qi Zhou. Genome editing in large animals:current status and future prospects. 国家科学评论:英文版[J]. 2019, 6(3): 402-420, https://www.webofscience.com/wos/woscc/full-record/WOS:000473767700015.
[24] Zhang, Ying, Hong, Qianlong, Cao, Chunwei, Yang, Lizhu, Li, Yongshun, Hai, Tang, Zhang, Hongyong, Zhou, Qi, Sui, Ruifang, Zhao, Jianguo. A novel porcine model reproduces human oculocutaneous albinism type. CELL DISCOVERYnull. 2019, 5: http://apps.webofknowledge.com/CitedFullRecord.do?product=UA&colName=WOS&SID=5CCFccWmJJRAuMzNPjj&search_mode=CitedFullRecord&isickref=WOS:000490007400001.
[25] Tao, Cong, Wang, Yajun, Zhao, Ying, Pan, Jianfei, Fan, Yiping, Liang, Xiaojuan, Cao, Chunwei, Zhao, Jianguo, Petris, Michael J, Li, Kui, Wang, Yanfang. Adipocyte-specific disruption of ATPase copper transporting alpha in mice accelerates lipoatrophy. DIABETOLOGIA[J]. 2019, 62(12): 2340-2353, https://www.webofscience.com/wos/woscc/full-record/WOS:000500998600016.
[26] Qiu, Jingtao, Zhang, Jinglin, Zhou, Yewen, Li, Xin, Li, Hongjiao, Liu, Jiali, Gou, Kemian, Zhao, Jianguo, Cui, Sheng. MicroRNA-7 inhibits melatonin synthesis by acting as a linking molecule between leptin and norepinephrine signaling pathways in pig pineal gland. JOURNAL OF PINEAL RESEARCH[J]. 2019, 66(3): https://www.webofscience.com/wos/woscc/full-record/WOS:000460583700005.
[27] Wang, Xiao, Cao, Chunwei, Li, Yongshun, Hai, Tang, Jia, Qitao, Zhang, Ying, Zheng, Qiantao, Yao, Jing, Qin, Guosong, Zhang, Hongyong, Song, Ruigao, Wang, Yanfang, Shui, Guanghou, Lam, Sin Man, Liu, Zhonghua, Wei, Hong, Meng, Anming, Zhou, Qi, Zhao, Jianguo. A harlequin ichthyosis pig model with a novel ABCA12 mutation can be rescued by acitretin treatment. JOURNAL OF MOLECULAR CELL BIOLOGY[J]. 2019, 11(12): 1029-1041, http://lib.cqvip.com/Qikan/Article/Detail?id=7100883412.
[28] Ji, XiaoJun, Chen, Wei, Wang, Xiao, Zhang, Yue, Liu, Qian, Guo, WeiWei, Zhao, JianGuo, Yang, ShiMing. Canalostomy is an ideal surgery route for inner ear gene delivery in big animal model. ACTA OTO-LARYNGOLOGICA[J]. 2019, 139(11): 939-947, https://www.webofscience.com/wos/woscc/full-record/WOS:000485163200001.
[29] 范一萍, 王晓梅, 贾启涛, 李成勃, 梁小娟, 曹春伟, 周磊, 陶聪, 赵建国, 刘剑锋, 王彦芳. 猪PRLR和FSHβ基因多态性检测及其与繁殖性状的关联分析. 中国畜牧兽医[J]. 2019, 46(3): 774-781, http://lib.cqvip.com/Qikan/Article/Detail?id=7001505133.
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[32] Sun, Xiuyun, Wang, Jun, Yao, Xia, Zheng, Wen, Mao, Yang, Lan, Tianlong, Wang, Liguo, Sun, Yonghui, Zhang, Xinyi, Zao, Qiuye, Zhao, Jianguo, Xiao, RuiPing, Zhang, Xiuqin, Ji, Guangju, Rao, Yu. A chemical approach for global protein knockdown from mice to non-human primates. CELL DISCOVERY[J]. 2019, 5(1): https://doaj.org/article/c439f61958e9443ea9425c06101d3ee2.
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[39] 黄娇娇, 曹春伟, 郑国民, 赵建国. 基因组编辑技术在猪遗传改良中的应用. 遗传[J]. 2017, 1078-1089, http://lib.cqvip.com/Qikan/Article/Detail?id=89679090504849554949484949.
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（1） 5hmc在猪早期胚胎发育和体细胞重编程中的研究，主持，部委级，2012-01--2016-12
（2） 规模化创造循环系统小型猪疾病模型，主持，部委级，2012-01--2015-12
（3） 功能性蛋白和mRNA介导的体细胞重编程，主持，院级级，2011-06--2015-05
（4） 用人工诱变的方法规模化挖掘猪的基因资源，主持，院级级，2011-01--2012-12
（5） 小分子化合物对体细胞核移植重编程作用机制的研究，主持，部委级，2011-01--2015-12
（6） 利用化学诱变创制出生缺陷等模型猪，主持，国家级，2011-01--2014-12
（7） 转基因技术改良荣昌猪脂肪性状及体细胞重编程机理，主持，院级级，2010-04--2013-04

（1） Genetic modifications in pigs for biomedicine，2015-07，Tang Hai
（2） 猪的遗传修饰和疾病模型的构建，全球华人遗传学大会暨全国第十三次医学遗传学学术会议，2014-10，Xianlong Wang
（3） Genetic modifications in pigs for creation of human disease models，2014-10，Chunwei Cao

已指导学生

黄娇娇  博士研究生  071008-发育生物学  

王亚朋  博士研究生  071008-发育生物学  

祁蒙  硕士研究生  071008-发育生物学  

罗霭玲  硕士研究生  071008-发育生物学  

现指导学生

杨锦修  博士研究生  071008-发育生物学  

张颖  博士研究生  071008-发育生物学  

张洪勇  硕士研究生  071008-发育生物学  

贾启涛  博士研究生  071008-发育生物学  

张蕊  硕士研究生  071008-发育生物学  

郑千涛  博士研究生  071008-发育生物学  

王霄  博士研究生  071008-发育生物学