单分子成像和超分辨光电融合成像技术开发及在生命科学研究中的应用。

2005-09--2010-07   中国科学院生物物理研究所   理学博士
2001-09--2005-07   华中科技大学   工学学士

   

2017-01~现在, 中国科学院生物物理研究所, 研究员级高级工程师
2012-01~2016-12,中国科学院生物物理研究所, 高级工程师
2010-08~2011-12,中国科学院生物物理研究所, 工程师

生物物理学实验

   

（1） 中国电子显微镜学会优秀青年学者奖, 其他, 2019

   

[1] Li, Weixing, Lu, Jing, Xiao, Ke, Zhou, Maoge, Li, Yuanyuan, Zhang, Xiang, Li, Zhixun, Gu, Lusheng, Xu, Xiaojun, Guo, Qiang, Xu, Tao, Ji, Wei. Integrated multimodality microscope for accurate and efficient target-guided cryo-lamellae preparation. NATURE METHODS[J]. 2023, 20(2): 268-+, http://dx.doi.org/10.1038/s41592-022-01749-z.
[2] Wu, Wanyan, Luo, Shihang, Fan, Chunyan, Yang, Tianjie, Zhang, Shuwen, Meng, Wenxiang, Xu, Tao, Ji, Wei, Gu, Lusheng. Tetra-color superresolution microscopy based on excitation spectral demixing. LIGHT-SCIENCE & APPLICATIONS[J]. 2023, 12(1): 99-108, http://dx.doi.org/10.1038/s41377-022-01054-6.
[3] Wang, Lei, Lu, Jing, Ji, Wei, Wan, Lingyu, Gu, Lusheng. Interferometrical single-molecule localization based on dynamic PSF engineering. OPTICS LETTERS[J]. 2022, 47(7): 1770-1773, http://dx.doi.org/10.1364/OL.453113.
[4] Yin, Jiaxiang, Meng, Hao, Lin, Jingfang, Ji, Wei, Xu, Tao, Liu, Huisheng. Pancreatic islet organoids-on-a-chip: how far have we gone?. JOURNAL OF NANOBIOTECHNOLOGY[J]. 2022, 20(1): http://dx.doi.org/10.1186/s12951-022-01518-2.
[5] Cai, Mingcheng, Guo, Zhongzhi, Shi, Fazhan, Li, Chunxing, Wang, Mengqi, Ji, Wei, Wang, Pengfei, Du, Jiangfeng. Parallel optically detected magnetic resonance spectrometer for dozens of single nitrogen-vacancy centers using laser-spot lattice. REVIEW OF SCIENTIFIC INSTRUMENTS[J]. 2021, 92(4): http://dx.doi.org/10.1063/5.0039110.
[6] Gu, Lusheng, Li, Yuanyuan, Zhang, Shuwen, Zhou, Maoge, Xue, Yanhong, Li, Weixing, Xu, Tao, Ji, Wei. Molecular-scale axial localization by repetitive optical selective exposure. NATURE METHODS[J]. 2021, 18(4): 369-+, http://dx.doi.org/10.1038/s41592-021-01099-2.
[7] Anwar, Shahzad, Zhang, Xiang, Ji, Wei, Xu, Xiaolan. Synthesis, characterization, and fluorescence study of tryptone-self-assembled and Zn(II)-coordinated nanoparticles. APPLIED NANOSCIENCE[J]. 2020, 10(5): 1571-1580, https://www.webofscience.com/wos/woscc/full-record/WOS:000518211000001.
[8] Wang, Lu, Liu, Zhilan, Wang, Lili, Wu, Qielan, Li, Xiang, Xie, Di, Zhang, Huimin, Zhang, Yongdeng, Gu, Lusheng, Xue, Yanhong, Yue, Ting, Liu, Gang, Ji, Wei, Wei, Haiming, Xu, Tao, Bai, Li. Spatial distribution of IL4 controls iNKT cell-DC crosstalk in tumors. CELLULAR & MOLECULAR IMMUNOLOGY[J]. 2020, 17(5): 496-506, https://www.webofscience.com/wos/woscc/full-record/WOS:000529703100006.
[9] Qi, Qingkai, Chi, Weijie, Li, Yuanyuan, Qiao, Qinglong, Chen, Jie, Miao, Lu, Zhang, Yi, Li, Jin, Ji, Wei, Xu, Tao, Liu, Xiaogang, Yoon, Juyoung, Xu, Zhaochao. A H-bond strategy to develop acid-resistant photoswitchable rhodamine spirolactams for super-resolution single-molecule localization microscopy. CHEMICAL SCIENCE[J]. 2019, 10(18): 4914-4922, http://cas-ir.dicp.ac.cn/handle/321008/171927.
[10] Gu, Lusheng, Li, Yuanyuan, Zhang, Shuwen, Xue, Yanhong, Li, Weixing, Li, Dong, Xu, Tao, Ji, Wei. Molecular resolution imaging by repetitive optical selective exposure. NATURE METHODS[J]. 2019, 16(11): 1114-+, [11] 李艳君, Xue YH, Xu XJ, Wang GP, Liu YQ, 吴浩, 李文辉, 王悦颖, 陈子恒, 张卫林, Zhu YS, Ji W, Xu T, 刘垒, 陈佺. A mitochondrial FUNDC1/HSC70 interaction organizes the proteostatic stress response at the risk of cell morbidity. EMBO JOURNAL[J]. 2019, 38(3): 1-20, http://dx.doi.org/10.15252/embj.201798786.
[12] Xu, Xiaojun, Xue, Yanhong, Tian, Buyun, Feng, Fengping, Gu, Lusheng, Li, Weixing, Ji, Wei, Xu, Tao. Ultra-stable super-resolution fluorescence cryo-microscopy for correlative light and electron cryo-microscopy. SCIENCE CHINA-LIFE SCIENCES[J]. 2018, 61(11): 1312-1319, http://lib.cqvip.com/Qikan/Article/Detail?id=6100053400.
[13] 王宏达, 李栋, 纪伟, 张名姝, 周正洪, 白丽, 储开芹, 张隆华, 孙育杰. 超高时空分辨蛋白质机器动态成像. 中国基础科学[J]. 2018, 20(1): 25-29, http://lib.cqvip.com/Qikan/Article/Detail?id=675432590.
[14] Xiaojun Xu, Yanhong Xue, Buyun Tian, Fengping Feng, Lusheng Gu, Weixing Li, Wei Ji, Tao Xu. Ultra-stable super-resolution fluorescence cryo-microscopy for correlative light and electron cryo-microscopy. 中国科学:生命科学英文版[J]. 2018, 61(11): 1312-1319, http://lib.cqvip.com/Qikan/Article/Detail?id=6100053400.
[15] Zou, Wenjuan, Fu, Jiajun, Zhang, Haining, Du, Kang, Huang, Wenming, Yu, Junwei, Li, Shitian, Fan, Yuedan, Baylis, Howard A, Gao, Shangbang, Xiao, Rui, Ji, Wei, Kang, Lijun, Xu, Tao. Decoding the intensity of sensory input by two glutamate receptors in one C-elegans interneuron. NATURE COMMUNICATIONS[J]. 2018, 9(1): https://doaj.org/article/124da2b989584319b6bc456cb179f4ab.
[16] Li WeiXing, Gu LuSheng, Xu XiaoJun, Xue YanHong, Ji Wei, Xu Tao. Cryogenic Super-resolution Correlative Light and Electron Microscopy (csCLEM): New Challenges and New Opportunities. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS[J]. 2018, 45(9): 957-960, https://www.webofscience.com/wos/woscc/full-record/WOS:000445847600007.
[17] Li WeiXing, Gu LuSheng, Xu XiaoJun, Xue YanHong, Ji Wei, Xu Tao. Cryogenic Super-resolution Correlative Light and Electron Microscopy (csCLEM): New Challenges and New Opportunities. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS[J]. 2018, 45(9): 957-960, https://www.webofscience.com/wos/woscc/full-record/WOS:000445847600007.
[18] Lan, Xi, Fan, Chunyan, Ji, Wei, Tian, Fuyun, Xu, Tao, Gao, Zhaobing. Grafting Voltage Sensing Sensitivity and Pharmacology in Potassium Channels. BIOPHYSICAL JOURNALnull. 2017, 112(3): 246A-246A, http://dx.doi.org/10.1016/j.bpj.2016.11.1347.
[19] Fan ChunYan, Li YuanYuan, Gu LuSheng, Li WeiXing, Xu XiaoJun, Zhang ShuWen, Wang Lei, Chen Yan, Xue YanHong, Ji Wei. Study on The Relationship of Laser Power Density and Localization Precision in PALM Imaging. PROGRESS IN BIOCHEMISTRY AND BIOPHYSICS[J]. 2017, 44(6): 534-536, https://www.webofscience.com/wos/woscc/full-record/WOS:000404091900009.
[20] 徐涛. Grafting voltage and pharmacological sensitivity in potassium channels. CELL RESEARCH[J]. 2016, 26(8): 935-945, http://www.corc.org.cn/handle/1471x/2374482.
[21] 徐涛. Three-dimensional super-resolution protein localization correlated with vitrified cellular context. SCIENTIFIC REPORTS[J]. 2015, 5: 13017-13027, [22] Ji, Wei, Liu, Bei, Xue, Yanhong, Zhao, Wei, Xu, Tao. Three-Dimensional Super-Resolution Protein Localization Correlated with Vitrified Cellular Context. BIOPHYSICAL JOURNALnull. 2015, 108(2): 475A-475A, https://www.webofscience.com/wos/woscc/full-record/WOS:000362849600002.
[23] 纪伟, 徐涛, 刘贝. 光学超分辨荧光显微成像——2014年诺贝尔化学奖解析. 自然杂志[J]. 2014, 36(6): 404-408, http://lib.cqvip.com/Qikan/Article/Detail?id=663667249.
[24] Liu, Bei, Feng, Wanjuan, Xu, Tao, Ji, Wei. A machine learning-based method to detect fluorescent spots and an accelerated, parallel implementation of this method. CHINESE SCIENCE BULLETIN[J]. 2014, 59(28): 3573-3578, https://www.webofscience.com/wos/woscc/full-record/WOS:000341837300009.
[25] 徐涛. High-density 3D single molecular analysis based on compressed sensing. BIOPHYSICAL JOURNAL[J]. 2014, 106(11): 2443-2449, https://www.webofscience.com/wos/woscc/full-record/WOS:000337012300018.
[26] 徐涛. Ultrafast, accurate, and robust localization of anisotropic dipoles. PROTEIN & CELL[J]. 2013, 4(8): 598-606, http://www.irgrid.ac.cn/handle/1471x/756662.
[27] Zhang YongDeng, Chang Hao, Gu LuSheng, Zhao YanHua, Xu Tao, Ji Wei. Super-resolution microscopy of live cells using single molecule localization. CHINESE SCIENCE BULLETIN[J]. 2013, 58(36): 4519-4527, https://www.webofscience.com/wos/woscc/full-record/WOS:000327482400001.
[28] Yang, Lu, Ji, Wei, Xue, Yanhong, Chen, Liangyi. Imaging beta-cell mass and function in situ and in vivo. JOURNAL OF MOLECULAR MEDICINE-JMMnull. 2013, 91(8): 929-938, https://www.webofscience.com/wos/woscc/full-record/WOS:000322581800004.
[29] 柳林, 李照玉, 纪伟. Dynamin在胞吞中的研究进展. 现代生物医学进展[J]. 2012, 12(1): 183-185, http://lib.cqvip.com/Qikan/Article/Detail?id=41100125.
[30] Xu, Pingyong, Zhang, Mingshu, Chang, Hao, Zhang, Yongdeng, Ji, Wei, Xu, Tao. Novel Photoconvertible Fluorescent Proteins for Super-Resolution Imaging. BIOPHYSICAL JOURNALnull. 2012, 102(3): 723A-723A, https://www.webofscience.com/wos/woscc/full-record/WOS:000321561205128.
[31] 柳林, 纪伟. 纤毛疾病和与之相关的基因. 现代生物医学进展[J]. 2012, 12(2): 373-376, http://lib.cqvip.com/Qikan/Article/Detail?id=41277008.
[32] 徐涛. Rational design of true monomeric and bright photoactivatable fluorescent proteins. NATURE METHODS[J]. 2012, 9(7): 727-U297, http://www.irgrid.ac.cn/handle/1471x/756906.
[33] Chang, Hao, Zhang, Mingshu, Ji, Wei, Chen, Juanjuan, Zhang, Yongdeng, Liu, Bei, Lu, Jingze, Zhang, Junlong, Xu, Pingyong, Xu, Tao. A unique series of reversibly switchable fluorescent proteins with beneficial properties for various applications. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA[J]. 2012, 109(12): 4455-4460, http://dx.doi.org/10.1073/pnas.1113770109.
[34] Li Zhengzheng, Liu Lin, Deng Yongqiang, Ji Wei, Du Wen, Xu Pingyong, Chen Liangyi, Xu Tao. Graded activation of CRAC channel by binding of different numbers of STIM1 to Orail subunits. 细胞研究：英文版[J]. 2011, 21(2): 305-315, http://lib.cqvip.com/Qikan/Article/Detail?id=37538305.
[35] Zhengzheng Li, Lin Liu, Yongqiang Deng, Wei Ji, Wen Du, Pingyong Xu, Liangyi Chen, Tao Xu. Graded activation of CRAC channel by binding of different numbers of STIM1 to Orail subunits. 细胞研究：英文版[J]. 2011, 21(2): 305-315, http://lib.cqvip.com/Qikan/Article/Detail?id=37538305.
[36] 徐涛. Graded activation of CRAC channel by binding of different numbers of STIM1 to Orai1 subunits. CELL RESEARCH[J]. 2011, 21(2): 305-315, http://www.irgrid.ac.cn/handle/1471x/757287.
[37] Chen, Yu, Wang, Yan, Ji, Wei, Xu, Pingyong, Xu, Tao. A Pre-Docking Role for Microtubules in Insulin-Stimulated Glucose Transporter 4 Translocation. BIOPHYSICAL JOURNALnull. 2010, 98(3): 670A-670A, https://www.webofscience.com/wos/woscc/full-record/WOS:000208762006369.
[38] Ji, Wei, Xu, Pingyong, Li, Zhengzheng, Lu, Jingze, Liu, Lin, Zhan, Yi, Chen, Yu, Hille, Bertil, Xu, Tao, Chen, Liangyi. Functional Stoichiometry Of The Unitary Calcium-release-activated Calcium Channel Revealed By Single-molecule Imaging. BIOPHYSICAL JOURNALnull. 2009, 96(3): 560A-560A, https://www.webofscience.com/wos/woscc/full-record/WOS:000426354000954.
[39] Wang Yan, Zhang JinZhong, Chen Yu, Jiang Li, Ji Wei, Xu Tao. Characterization of GLUT4-containing vesicles in 3T3-L1 adipocytes by total internal reflection fluorescence microscopy. SCIENCE IN CHINA SERIES C-LIFE SCIENCES[J]. 2009, 52(7): 665-671, http://www.irgrid.ac.cn/handle/1471x/757927.
[40] 徐涛. A pre-docking role for microtubules in insulin-stimulated glucose transporter 4 translocation. FEBS JOURNAL[J]. 2008, 275(4): 705-712, http://www.irgrid.ac.cn/handle/1471x/757750.
[41] Ji, Wei, Xu, Pingyong, Li, Zhengzheng, Lu, Jingze, Liu, Lin, Zhan, Yi, Chen, Yu, Hille, Bertil, Xu, Tao, Chen, Liangyi. Functional stoichiometry of the unitary calcium-release-activated calcium channel. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA[J]. 2008, 105(36): 13668-13673, http://www.irgrid.ac.cn/handle/1471x/757622.