研究方向
1. 虫媒病毒—植物—介体昆虫互作的分子机理；2. 油料作物基因组学

研究内容及意义

1. 虫媒病毒是多种人类、动物和农作物病害的致病病原，是影响人类和动物健康、社会稳定和粮食安全的重要不利因素。以往研究植物病毒病害往往注重于病毒和植物寄主二个方面，而实际上参与病害发生及防治的生物因子还有传毒昆虫介体、植物和昆虫介体内的微生物种群等等，研究这些多元生物及环境因子的互作并对病毒发生的流行成灾机制，不但能对多界生物互作中蕴含的科学现象发现其分子水平的规律，也为设计高效、绿色防控的新手段，为我国粮食安全的有利保障。我们选取我国农业生产中重要的虫媒植物病毒作为研究对象，重要经济作物和模式植物为研究材料，剖析介体昆虫—病毒—共生菌群落—寄主植物多元互作机制，探索防虫抗病新理论，注重从生态学和病毒病害田间自然体系，研究上述复杂系统中多因素之间的相互影响、相互制约关系；综合利用多者关系，结合利用农业大数据的理论与技术，应用于作物的分子设计，培育高抗、高附加值新品种。目前我们主要探索植物免疫受体识别以及跨界RNA分子在三者互作的作用分子机制。

2. 植物脂肪酸和油脂代谢相关遗传因子的发掘和功能鉴定，并应用于油料作物的分子品种设计，培育优质、高产新品种。

   

071007-遗传学

病毒分子遗传学，植物分子生物学，虫媒病毒学

2000-07--2007-06   中国科学院微生物研究所   研究生院生物系学生会主席/研究生 遗传学博士
1996-09--2000-06   浙江大学   院系学生会主席，植保系团总支副书记/本科农学学士

2000-07--2007-06   中国科学院微生物研究所   遗传学博士
1996-09--2000-06   浙江大学   农学学士

2000-07--2007-06   中国科学院微生物研究所   遗传学博士
1996-09--2000-06   浙江大学   农学学士

   

2013-04~现在, 新加坡国立大学 淡马锡生命科学研究院, Principal Investigator, Assistant Director
2011-01~2013-03,新加坡国立大学 淡马锡生命科学研究院, Group leader, Biotechnology Manager
2009-04~2010-12,新加坡国立大学 淡马锡生命科学研究院, Research Fellow
2007-07~2009-03,新加坡国立大学 淡马锡生命科学研究院, 博士后研究员

   

[1] 叶健, 郝小江, 李玉林, 孙艳伟, 赵平芝, 黄烈军. C21-孕甾烷及其衍生物在抗植物病毒中的应用. CN: CN112514907A, 2021-03-19.

[2] 叶健, 徐爽. 蛋白质WRKY78在调控植物生物胁迫抗性中的应用. CN: CN111826390A, 2020-10-27.

[3] 叶健, 龚雨晴, 赵平芝. 拟南芥转录因子基因PIF3在植物抗虫胁迫中的应用. CN: CN110396125A, 2019-11-01.

[4] 叶健, 孙艳伟. 一种提高植物对双生病毒抗性的方法. CN: CN108277228A, 2018-07-13.

[5] 叶健, 姚香梅, 龚雨晴, 赵启祥. 一株嗜油不动杆菌NMB17及其在植物病害防治中的应用. CN: CN108220211A, 2018-06-29.

[6] 叶健, 孙艳伟. 通过过表达JcARF19基因来提高小桐子果实产量的方法. CN: CN107760708A, 2018-03-06.

[7] 叶健, 姚香梅, 王端. 荧光假单胞菌pf27在抗烟粉虱和防治马铃薯病害中的应用. CN: CN107251909A, 2017-10-17.

[8] 叶健, 姚香梅, 王端. 一株荧光假单胞菌pf27及其在植物促生中的应用. CN: CN107236689A, 2017-10-10.

   

[1] Li, YuLin, Zhang, Yu, Zhao, PingZhi, Hu, ZhanXing, Gu, YuCheng, Ye, Jian, Hao, XiaoJiang. Two new steroidal alkaloids from the rhizomes of Veratrum nigrum L. and their anti-TYLCV activity. FITOTERAPIA[J]. 2020, 147: http://dx.doi.org/10.1016/j.fitote.2020.104731.

[2] 张璇, 叶健. 基于三者互作解析的虫媒植物病害前沿防控技术研发策略. 中国科学基金[J]. 2020, 34(4): 477-485, http://lib.cqvip.com/Qikan/Article/Detail?id=7102759867.

[3] Jian Ye, Lili Zhang, Xuan Zhang, Rongxiang Fang. Plant defense networks against insect-borne pathogens.. Trends Plant Sci[J]. 2020, 
[4] 叶健. 农作物病毒病害绿色防控技术创新. 科技促进发展[J]. 2019, 362-368, http://lib.cqvip.com/Qikan/Article/Detail?id=75746774504849574852484855.

[5] Ye, Jian, Wang, Chunming, Sun, Yanwei, Qu, Jing, Mao, Huizhu, Chua, NamHai. Overexpression of a Transcription Factor Increases Lipid Content in a Woody Perennial Jatropha curcas. FRONTIERS IN PLANT SCIENCE[J]. 2018, 9: https://doaj.org/article/1d0b5ba127ba4ddb826fb6bedc272ef6.

[6] Jin, Jingjing, Sun, Yanwei, Qu, Jing, Syah, Rahmad, Lim, ChinHuat, Alfiko, Yuzer, Rahman, Nur Estya Bte, Suwanto, Antonius, Yue, Genhua, Wong, Limsoon, Chua, NamHai, Ye, Jian. Transcriptome and functional analysis reveals hybrid vigor for oil biosynthesis in oil palm (vol 7, 439, 2017). SCIENTIFIC REPORTSnull. 2018, 8: https://www.webofscience.com/wos/woscc/full-record/WOS:000448272600001.

[7] 王端, 姚香梅, 叶健. 根际微生物-植物-病毒-介体昆虫多元互作研究进展. 生物技术通报[J]. 2018, 34(2): 54-65, http://lib.cqvip.com/Qikan/Article/Detail?id=674831099.

[8] Sun, Yanwei, Wang, Chunming, Wang, Ning, Jiang, Xiyuan, Mao, Huizhu, Zhu, Changxiang, Wen, Fujiang, Wang, Xianghua, Lu, Zhijun, Yue, Genhua, Xu, Zengfu, Ye, Jian. Manipulation of Auxin Response Factor 19 affects seed size in the woody perennial Jatropha curcas. SCIENTIFIC REPORTS[J]. 2017, 7(7): http://ir.xtbg.org.cn/handle/353005/10299.

[9] 叶健, 龚雨晴, 方荣祥. 病毒-昆虫-植物三者互作研究进展及展望. 中国科学院院刊[J]. 2017, 32(8): 845-855, http://www.chinaxiv.org/abs/201708.00234.

[10] 叶健. Comparative transcriptomic and functional analysis reveals an EAR-motif-containing R2R3 MYB that modulates phenylpropene biosynthesis in monocot plant oil palm. BMC plant biology. 2017, 
[11] 叶健. 病毒—媒介昆虫—植物三者互作研究进展及展望. 中国科学院院刊. 2017, 
[12] Li, Ran, Reddy, Vaishnavi Amarr, Jin, Jingjing, Rajan, Chakaravarthy, Wang, Qian, Yue, Genhua, Lim, Chin Huat, Chua, NamHai, Ye, Jian, Sarojam, Rajani. Comparative transcriptome analysis of oil palm flowers reveals an EAR-motif-containing R2R3-MYB that modulates phenylpropene biosynthesis. BMC PLANT BIOLOGY[J]. 2017, 17(1): http://dx.doi.org/10.1186/s12870-017-1174-4.

[13] Jin, Jingjing, Sun, Yanwei, Qu, Jing, Syah, Rahmad, Lim, ChinHuat, Alfiko, Yuzer, Rahman, NurEstyaBte, Suwanto, Antonius, Yue, Genhua, Wong, Limsoon, Chua, NamHai, Ye, Jian. Transcriptome and functional analysis reveals hybrid vigor for oil biosynthesis in oil palm. SCIENTIFIC REPORTS[J]. 2017, 7(1): https://doaj.org/article/d3629f745c464c8ea55d1e6bf349931e.

[14] 叶健. CURLY LEAF Regulates Gene Sets Coordinating Seed Size and Lipid Biosynthesis in Arabidopsis. Plant Physiology. 2016, 
[15] Tang, Weijie, Wu, Tingting, Ye, Jian, Sun, Juan, Jiang, Yue, Yu, Jun, Tang, Jianpeng, Chen, Gaoming, Wang, Chunming, Wan, Jianmin. SNP-based analysis of genetic diversity reveals important alleles associated with seed size in rice. BMC PLANT BIOLOGY[J]. 2016, 16(1): http://dx.doi.org/10.1186/s12870-016-0779-3.

[16] Zhao, Pingzhi, Sokolov, Lubomir N, Ye, Jian, Tang, ChengYi, Shi, Jisen, Zhen, Yan, Lan, Wenzhi, Hong, Zhi, Qi, Jinliang, Lu, GuiHua, Pandey, Girdhar K, Yang, YongHua. The LIKE SEX FOUR2 regulates root development by modulating reactive oxygen species homeostasis in Arabidopsis. SCIENTIFIC REPORTS[J]. 2016, 6: https://www.webofscience.com/wos/woscc/full-record/WOS:000378865800001.

[17] 叶健. Towards sustainable protection against insect-borne plant viral diseases: phytohormones and beyond. Science Foundation in China. 2016, 
[18] Tang, Weijie, Wu, Tingting, Ye, Jian, Sun, Juan, Jiang, Yue, Yu, Jun, Tang, Jianpeng, Chen, Gaoming, Wang, Chunming, Wan, Jianmin. SNP-based analysis of genetic diversity reveals important alleles associated with seed size in rice (vol 16, pg 93, 2016). BMC PLANT BIOLOGYnull. 2016, 16: https://www.webofscience.com/wos/woscc/full-record/WOS:000377267900001.

[19] Jin, Jingjing, Lee, May, Bai, Bin, Sun, Yanwei, Qu, Jing, Rahmadsyah, Alfiko, Yuzer, Lim, Chin Huat, Suwanto, Antonius, Sugiharti, Maria, Wong, Limsoon, Ye, Jian, Chua, NamHai, Yue, Gen Hua. Draft genome sequence of an elite Dura palm and whole-genome patterns of DNA variation in oil palm. DNA RESEARCH[J]. 2016, 23(6): 527-533, https://www.webofscience.com/wos/woscc/full-record/WOS:000392723300003.

[20] Weijie Tang, Tingting Wu, Jian Ye, Juan Sun, Yue Jiang, Jun Yu, Jianpeng Tang, Gaoming Chen, Chunming Wang, Jianmin Wan. Erratum to: SNP-based analysis of genetic diversity reveals important alleles associated with seed size in rice. BMC Plant Biology. 2016, 16(1): http://dx.doi.org/10.1186/s12870-016-0801-9.

[21] Sun, YanWei, Tee, ChuanSia, Ma, YongHuan, Wang, Gang, Yao, XiangMei, Ye, Jian. Attenuation of Histone Methyltransferase KRYPTONITE-mediated transcriptional gene silencing by Geminivirus. SCIENTIFIC REPORTS[J]. 2015, 5: https://www.webofscience.com/wos/woscc/full-record/WOS:000365272100001.

[22] Li, Ran, Tee, ChuanSia, Jiang, YuLin, Jiang, XiYuan, Venkatesh, Prasanna Nori, Sarojam, Rajani, Ye, Jian. A terpenoid phytoalexin plays a role in basal defense of Nicotiana benthamiana against Potato virus X. SCIENTIFIC REPORTS[J]. 2015, 5: 
[23] Wang, Huan, Niu, QiWen, Wu, HuiWen, Liu, Jun, Ye, Jian, Yu, Niu, Chua, NamHai. Analysis of non-coding transcriptome in rice and maize uncovers roles of conserved lncRNAs associated with agriculture traits. PLANT JOURNAL[J]. 2015, 84(2): 404-416, http://dx.doi.org/10.1111/tpj.13018.

[24] Ye, Jian, Yang, Junyi, Sun, Yanwei, Zhao, Pingzhi, Gao, Shiqiang, Jung, Choonkyun, Qu, Jing, Fang, Rongxiang, Chua, NamHai. Geminivirus Activates ASYMMETRIC LEAVES 2 to Accelerate Cytoplasmic DCP2-Mediated mRNA Turnover and Weakens RNA Silencing in Arabidopsis. PLOS PATHOGENS[J]. 2015, 11(10): http://dx.doi.org/10.1371/journal.ppat.1005196.

[25] Lee, May, Xia, Jun Hong, Zou, Zhongwei, Ye, Jian, Rahmadsyah, Alfiko, Yuzer, Jin, Jingjing, Lieando, Jessica Virginia, Purnamasari, Maria Indah, Lim, Chin Huat, Suwanto, Antonius, Wong, Limsoon, Chua, NamHai, Yue, Gen Hua. A consensus linkage map of oil palm and a major QTL for stem height. SCIENTIFIC REPORTS[J]. 2015, 5: https://www.webofscience.com/wos/woscc/full-record/WOS:000348768100004.

[26] Ye, Jian, Liu, Peng, Zhu, Chengsong, Qu, Jing, Wang, Xianghua, Sun, Yanwei, Sun, Fei, Jiang, Yulin, Yue, Genhua, Wang, Chunming. Identification of candidate genes JcARF19 and JcIAA9 associated with seed size traits in Jatropha. FUNCTIONAL & INTEGRATIVE GENOMICS[J]. 2014, 14(4): 757-766, https://www.webofscience.com/wos/woscc/full-record/WOS:000345085800012.

[27] Ye, Jian, Geng, Yunfeng, Zhang, Bipei, Mao, Huizhu, Qu, Jing, Chua, NamHai. The Jatropha FT ortholog is a systemic signal regulating growth and flowering time. BIOTECHNOLOGY FOR BIOFUELS[J]. 2014, 7(1): http://ir.xtbg.org.cn/handle/353005/7257.

[28] Li, Ran, Weldegergis, Berhane T, Li, Jie, Jung, Choonkyun, Qu, Jing, Sun, Yanwei, Qian, Hongmei, Tee, ChuanSia, van Loon, Joop J A, Dicke, Marcel, Chua, NamHai, Liu, ShuSheng, Ye, Jian. Virulence Factors of Geminivirus Interact with MYC2 to Subvert Plant Resistance and Promote Vector Performance. PLANT CELL[J]. 2014, 26(12): 4991-5008, http://dx.doi.org/10.1105/tpc.114.133181.

[29] Wang, Gang, Sun, YanWei, Xu, RuiRui, Qu, Jing, Tee, ChuanSia, Jiang, XiYuan, Ye, Jian. DNA-A of a highly pathogenic Indian cassava mosaic virus isolated from Jatropha curcas causes symptoms in Nicotiana benthamiana. VIRUS GENES[J]. 2014, 48(2): 402-405, https://www.webofscience.com/wos/woscc/full-record/WOS:000333213500027.

[30] Ran Li, Berhane T Weldegergis, Jie Li, Choonkyun Jung, Jing Qu, Yanwei Sun, Hongmei Qian, ChuanSia Tee, Joop JA van Loon, Marcel Dicke, NamHai Chua, ShuSheng Liu, Jian Ye. Virulence Factors of Geminivirus Interact with MYC2 to Subvert Plant Resistance and Promote Vector Performance C W. The Plant Cell. 2014, 26(12): 4991-5008, https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4311212/.

[31] Ye, Jian, Qu, Jing, Mao, HuiZhu, Ma, ZhiGang, Rahman, Nur Estya Binte, Bai, Chao, Chen, Wen, Jiang, ShuYe, Ramachandran, Srinivasan, Chua, NamHai. Engineering geminivirus resistance in Jatropha curcus. BIOTECHNOLOGY FOR BIOFUELS[J]. 2014, 7(1): http://ir.xtbg.org.cn/handle/353005/7310.

[32] Wang, Chunming, Ye, Jian, Tang, Weijie, Liu, Zhiyi, Zhu, Chengsong, Wang, Maoqing, Wan, Jianmin. Loop Nucleotide Polymorphism in a Putative miRNA Precursor Associated with Seed Length in Rice (Oryza sativa L.). INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES[J]. 2013, 9(6): 578-586, https://www.webofscience.com/wos/woscc/full-record/WOS:000321284800006.

[33] Wang, Chun Ming, Liu, Peng, Sun, Fei, Li, Lei, Liu, Peng, Ye, Jian, Yue, Gen Hua. Isolation and Identification of miRNAs in Jatropha curcas. INTERNATIONAL JOURNAL OF BIOLOGICAL SCIENCES[J]. 2012, 8(3): 418-429, https://doaj.org/article/6690756f96bf45c7aec04243440c4006.

[34] Qu Jing, Mao HuiZhu, Chen Wen, Gao ShiQiang, Bai YaNan, Sun YanWei, Geng YunFeng, Ye Jian. Development of marker-free transgenic Jatropha plants with increased levels of seed oleic acid. Biotechnology for Biofuels[J]. 2012, 5(1): https://doaj.org/article/095f98ff35bd4a85b5756b0b76e8eb1a.

[35] 叶健. Dissecting functions of KTN and WRI1 in cotton fiber development by Virus-induced gene silencing. Plant Physiology. 2012, 
[36] Qu, Jing, Ye, Jian, Geng, YunFeng, Sun, YanWei, Gao, ShiQiang, Zhang, BiPei, Chen, Wen, Chua, NamHai. Dissecting Functions of KATANIN and WRINKLED1 in Cotton Fiber Development by Virus-Induced Gene Silencing. PLANT PHYSIOLOGY[J]. 2012, 160(2): 738-748, http://dx.doi.org/10.1104/pp.112.198564.

[37] Sun, Fei, Liu, Peng, Ye, Jian, Lo, Loong Chuan, Cao, Suying, Li, Lei, Yue, Gen Hua, Wang, Chun Ming. An approach for jatropha improvement using pleiotropic QTLs regulating plant growth and seed yield. BIOTECHNOLOGY FOR BIOFUELS[J]. 2012, 5(1): 42-42, http://ir.xtbg.org.cn/handle/353005/7714.

[38] Qu, Jing, Mao, HuiZhu, Chen, Wen, Gao, ShiQiang, Bai, YaNan, Sun, YanWei, Geng, YunFeng, Ye, Jian. Development of marker-free transgenic Jatropha plants with increased levels of seed oleic acid. BIOTECHNOLOGY FOR BIOFUELS[J]. 2012, 5(1): 10-10, http://ir.xtbg.org.cn/handle/353005/7498.

[39] Gao, ShiQiang, Qu, Jing, Chua, NamHai, Ye, Jian. A new strain of Indian cassava mosaic virus causes a mosaic disease in the biodiesel crop Jatropha curcas. ARCHIVES OF VIROLOGY[J]. 2010, 155(4): 607-612, 
[40] Ye, Jian, Qu, Jing, Bui, Ha Thi Ngoc, Chua, NamHai. Rapid analysis of Jatropha curcas gene functions by virus-induced gene silencing. PLANT BIOTECHNOLOGY JOURNAL[J]. 2009, 7(9): 964-976, http://ir.xtbg.org.cn/handle/353005/7126.

[41] 方荣祥. DNA-templated CMV viral capsid proteins assemble into nanotubes. Chemical Communications[J]. 2008, 1(1): 49-51, https://www.webofscience.com/wos/woscc/full-record/WOS:000251633000004.

[42] 叶健. 黄瓜花叶病毒基因沉默抑制子2b蛋白的结构和功能. 2007, 116-, http://124.16.144.217/handle/311023/3557.

[43] 方荣祥. Artificial MicroRNA-Mediated Virus Resistance in Plants. Journal of Virology[J]. 2007, 81(12): 6690-6699, http://124.16.144.217/handle/311023/1391.

[44] Zhang Peng, Zhang Jian ye, Sun Yong, Li Xue ren, Zhang Zonglin, Cheng DZ, Wu M. Limited memory fusion of multi-sensor based on support degree. PROCEEDINGS OF THE 26TH CHINESE CONTROL CONFERENCE, VOL 3null. 2007, 174-+, http://apps.webofknowledge.com/CitedFullRecord.do?product=UA&colName=WOS&SID=5CCFccWmJJRAuMzNPjj&search_mode=CitedFullRecord&isickref=WOS:000251010500038.

[45] 方荣祥. Efficient depletion of multiple SARS-CoV mRNAs by a single small interfering RNA targeting the leader sequence. Progress in Biochemistry and Biophysics[J]. 2007, 34(10): 1092-1100, http://124.16.144.217/handle/311023/3264.

（ 1 ） 利用人工小RNA提高植物抗病毒活性, Artificial MicroRNAs for Plant Virus Resistance, Humana, 2012-06, 第 2 作者
（ 2 ） 小桐子油的遗传工程改进, Improvement of Jatropha oil by genetic transformation, Springer, 2012-09, 第 1 作者
Lipid biosynthesis and regulation in Jatropha curcas, an emerging model for woody energy plants, Springer Science Publishers, 2017-04, 第 2 作者
Jatropha breeding, Springer Science Publishers, 2017-04, 第 1 作者

   

（ 1 ） 中科院先导B方向, 主持, 部委级, 2014-05--2019-06
（ 2 ） 植物病毒病害, 主持, 国家级, 2016-01--2018-12
（ 3 ） 番茄双生病毒利用寄主细胞质mRNA降解系统增强致病性的分子机制, 主持, 国家级, 2017-01--2020-12

现指导学生

马永焕  02  63228  

龚雨晴  02  63228  

徐爽   02  63228